(Circular) (Six-base) MAPSORT of: pFN21AB7306.seq Check: 1925 from: 1 to: 7897 pFN21AB7306 7897 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3209 3419 3809 3209 Size: 210 390 7297 Fragments arranged by size: 7297 390 210 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5707 278 Size: 53 83 186 936 4171 2468 Fragments arranged by size: 4171 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5240 1937 Size: 3303 4594 Acc65I G'GTAC_C Cuts at: 5218 5218 Size: 7897 AclI AA'CG_TT Cuts at: 1563 6024 6397 1563 Size: 4461 373 3063 Fragments arranged by size: 4461 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2874 3066 5960 7344 7612 1632 Size: 1242 192 2894 1384 268 1917 Fragments arranged by size: 2894 1917 1384 1242 268 192 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7700 AflIII A'CryG_T Cuts at: 1203 4322 4854 6796 7773 1203 Size: 3119 532 1942 977 1327 Fragments arranged by size: 3119 1942 1327 977 532 AgeI A'CCGG_T Cuts at: 6728 6728 Size: 7897 AhdI GACnn_n'nnGTC Cuts at: 6626 6626 Size: 7897 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7897 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3118 1184 Size: 1934 5963 AlwNI CAG_nnn'CTG Cuts at: 3700 5640 7212 3700 Size: 1940 1572 4385 Fragments arranged by size: 4385 1940 1572 ApaI G_GGCC'C Cuts at: 3176 3386 3176 Size: 210 7687 ApaLI G'TGCA_C Cuts at: 2394 4814 5954 7110 2394 Size: 2420 1140 1156 3181 Fragments arranged by size: 3181 2420 1156 1140 ApoI r'AATT_y Cuts at: 1493 2099 2150 2649 2793 2839 3497 3905 4556 Size: 606 51 499 144 46 658 408 651 Cuts at: 4556 4757 5206 5432 5584 7693 1493 Size: 201 449 226 152 2109 1697 Fragments arranged by size: 2109 1697 658 651 606 499 449 408 226 201 152 144 51 46 AseI AT'TA_AT Cuts at: 160 1032 6451 160 Size: 872 5419 1606 Fragments arranged by size: 5419 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7897 AvaI C'yCGr_G Cuts at: 1870 1943 4170 4371 5222 1870 Size: 73 2227 201 851 4545 Fragments arranged by size: 4545 2227 851 201 73 BamHI G'GATC_C Cuts at: 5227 5594 5227 Size: 367 7530 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5218 6673 618 Size: 325 217 627 3431 1455 1842 Fragments arranged by size: 3431 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2263 3176 3386 5216 1391 Size: 872 913 210 1830 4072 Fragments arranged by size: 4072 1830 913 872 210 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2524 2573 3211 4009 4162 4491 4947 Size: 971 625 49 638 798 153 329 456 Cuts at: 4947 928 Size: 3878 Fragments arranged by size: 3878 971 798 638 625 456 329 153 49 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6088 6122 1903 Size: 34 4151 34 3678 Fragments arranged by size: 4151 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4312 5789 6999 4312 Size: 1477 1210 5210 Fragments arranged by size: 5210 1477 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7897 BfrBI ATG'CAT Cuts at: 2764 2980 3400 3577 2764 Size: 216 420 177 7084 Fragments arranged by size: 7084 420 216 177 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2754 3567 3975 Size: 107 122 71 631 605 1082 813 408 Cuts at: 3975 4626 6508 136 Size: 651 1882 1525 Fragments arranged by size: 1882 1525 1082 813 651 631 605 408 122 107 71 BglII A'GATC_T Cuts at: 7892 7892 Size: 7897 BlpI GC'TnA_GC Cuts at: 2598 5314 2598 Size: 2716 5181 Bme1580I G_kGCm'C Cuts at: 1790 2398 2782 3176 3386 4818 5958 7114 1790 Size: 608 384 394 210 1432 1140 1156 2573 Fragments arranged by size: 2573 1432 1156 1140 608 394 384 210 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7897 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4805 5628 6586 Size: 416 53 83 399 24 3388 823 958 Cuts at: 6586 442 Size: 1753 Fragments arranged by size: 3388 1753 958 823 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7897 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4267 6557 1239 Size: 3028 2290 2579 Fragments arranged by size: 3028 2579 2290 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4917 6022 6887 7185 4917 Size: 1105 865 298 5629 Fragments arranged by size: 5629 1105 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6560 882 Size: 617 5061 2219 Fragments arranged by size: 5061 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2199 4190 5688 493 Size: 711 995 1991 1498 2702 Fragments arranged by size: 2702 1991 1498 995 711 BsaBI GATnn'nnATC Cuts at: 4306 4637 7891 4306 Size: 331 3254 4312 Fragments arranged by size: 4312 3254 331 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5704 6086 6721 275 Size: 53 83 186 936 4171 382 635 1451 Fragments arranged by size: 4171 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6330 6728 7002 7149 7560 6330 Size: 398 274 147 411 6667 Fragments arranged by size: 6667 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3188 3218 3398 3428 3788 Size: 30 165 30 1785 30 180 30 360 Cuts at: 3788 3818 1178 Size: 30 5257 Fragments arranged by size: 5257 1785 360 180 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3320 4116 5159 1186 Size: 2134 796 1043 3924 Fragments arranged by size: 3924 2134 1043 796 BseYI C'CCAG_C Cuts at: 7100 7596 7100 Size: 496 7401 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3724 3925 4351 4576 4771 4879 1163 Size: 573 1988 201 426 225 195 108 4181 Fragments arranged by size: 4181 1988 573 426 225 201 195 108 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5347 6108 7136 1540 Size: 275 186 3346 761 1028 2301 Fragments arranged by size: 3346 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2263 2398 4818 5958 6043 7114 2263 Size: 135 2420 1140 85 1071 3046 Fragments arranged by size: 3046 2420 1140 1071 135 85 BsmI GAATG_Cn' Cuts at: 2416 5408 5501 2416 Size: 2992 93 4812 Fragments arranged by size: 4812 2992 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2263 2398 2782 3176 3386 4818 5216 Size: 399 473 135 384 394 210 1432 398 Cuts at: 5216 5958 6043 7114 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1432 1071 742 473 399 398 394 384 210 135 85 BspEI T'CCGG_A Cuts at: 7560 7560 Size: 7897 BspHI T'CATG_A Cuts at: 5089 5786 7516 5089 Size: 697 1730 5470 Fragments arranged by size: 5470 1730 697 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3209 3419 3809 5252 844 Size: 2365 210 390 1443 3489 Fragments arranged by size: 3489 2365 1443 390 210 BsrBI CCG'CTC Cuts at: 1394 1823 5784 1394 Size: 429 3961 3507 Fragments arranged by size: 3961 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2595 4537 6392 6566 65 Size: 1183 1347 1942 1855 174 1396 Fragments arranged by size: 1942 1855 1396 1347 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6541 6728 1700 Size: 110 4731 187 2869 Fragments arranged by size: 4731 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2720 96 Size: 2624 5273 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7897 BssSI C'ACGA_G Cuts at: 3051 5957 6969 3051 Size: 2906 1012 3979 Fragments arranged by size: 3979 2906 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2402 2594 3201 3411 3801 794 Size: 1608 192 607 210 390 4890 Fragments arranged by size: 4890 1608 607 390 210 192 BstBI TT'CG_AA Cuts at: 4561 4762 6701 4561 Size: 201 1939 5757 Fragments arranged by size: 5757 1939 201 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7897 BstYI r'GATC_y Cuts at: 1972 4543 5227 5594 5979 5996 7448 7534 7546 Size: 2571 684 367 385 17 1452 86 12 Cuts at: 7546 7892 1972 Size: 346 1977 Fragments arranged by size: 2571 1977 1452 684 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2743 3556 3790 3985 513 Size: 552 1678 813 234 195 4425 Fragments arranged by size: 4425 1678 813 552 234 195 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3915 487 Size: 683 56 2689 4469 Fragments arranged by size: 4469 2689 683 56 BtsI GCAGTG_nn' Cuts at: 2494 3112 3499 3712 4759 5413 6207 6227 2494 Size: 618 387 213 1047 654 794 20 4164 Fragments arranged by size: 4164 1047 794 654 618 387 213 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7897 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7862 DraI TTT'AAA Cuts at: 2305 2610 5034 5201 2305 Size: 305 2424 167 5001 Fragments arranged by size: 5001 2424 305 167 DraIII CAC_nnn'GTG Cuts at: 2402 2402 Size: 7897 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6904 809 Size: 507 5588 1802 Fragments arranged by size: 5588 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5056 5344 6233 Size: 54 1425 50 275 18 3226 288 889 Cuts at: 6233 7862 8 Size: 1629 43 Fragments arranged by size: 3226 1629 1425 889 288 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5344 1537 Size: 275 3532 4090 Fragments arranged by size: 4090 3532 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2231 3621 4047 4701 5827 1353 Size: 878 1390 426 654 1126 3423 Fragments arranged by size: 3423 1390 1126 878 654 426 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6484 6858 7004 387 Size: 1064 138 4895 374 146 1280 Fragments arranged by size: 4895 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2261 2261 Size: 7897 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2874 3066 4267 5960 6557 7344 7612 Size: 393 1242 192 1201 1693 597 787 268 Cuts at: 7612 1239 Size: 1524 Fragments arranged by size: 1693 1524 1242 1201 787 597 393 268 192 EcoNI CCTnn'n_nnAGG Cuts at: 4614 4614 Size: 7897 EcoO109I rG'GnC_Cy Cuts at: 2424 3172 3382 2424 Size: 748 210 6939 Fragments arranged by size: 6939 748 210 EcoRI G'AATT_C Cuts at: 2649 5206 5584 2649 Size: 2557 378 4962 Fragments arranged by size: 4962 2557 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4033 4033 Size: 7897 FspI TGC'GCA Cuts at: 6403 6403 Size: 7897 HaeII r_GCGC'y Cuts at: 1678 7044 1678 Size: 5366 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2452 2484 3855 3887 4190 Size: 32 135 32 880 32 1371 32 303 Cuts at: 4190 4222 4999 5031 6538 6570 6612 6644 1373 Size: 32 777 32 1507 32 42 32 2626 Fragments arranged by size: 2626 1507 1371 880 777 303 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5241 5487 669 Size: 988 281 3303 246 3079 Fragments arranged by size: 3303 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3119 4583 4881 4898 748 Size: 2371 1464 298 17 3747 Fragments arranged by size: 3747 2371 1464 298 17 HpaI GTT'AAC Cuts at: 5487 5487 Size: 7897 KpnI G_GTAC'C Cuts at: 5222 5222 Size: 7897 MfeI C'AATT_G Cuts at: 5496 5496 Size: 7897 MluI A'CGCG_T Cuts at: 7773 7773 Size: 7897 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7011 7195 1305 Size: 5706 184 2007 Fragments arranged by size: 5706 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5058 10 Size: 54 1425 343 3226 2849 Fragments arranged by size: 3226 2849 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3529 5855 6214 6373 518 Size: 648 2363 2326 359 159 2042 Fragments arranged by size: 2363 2326 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4164 4460 5313 5990 7138 7383 1606 Size: 2558 296 853 677 1148 245 2120 Fragments arranged by size: 2558 2120 1148 853 677 296 245 NcoI C'CATG_G Cuts at: 513 1065 3985 513 Size: 552 2920 4425 Fragments arranged by size: 4425 2920 552 NdeI CA'TA_TG Cuts at: 387 2380 3091 4624 387 Size: 1993 711 1533 3660 Fragments arranged by size: 3660 1993 1533 711 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7897 NotI GC'GGCC_GC Cuts at: 5344 5344 Size: 7897 NsiI A_TGCA'T Cuts at: 2766 2982 3402 3579 2766 Size: 216 420 177 7084 Fragments arranged by size: 7084 420 216 177 NspI r_CATG'y Cuts at: 1132 1229 3900 4326 4570 4858 5083 5182 5255 Size: 97 2671 426 244 288 225 99 73 Cuts at: 5255 6800 1132 Size: 1545 2229 Fragments arranged by size: 2671 2229 1545 426 288 244 225 99 97 73 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7897 PciI A'CATG_T Cuts at: 4322 4854 6796 4322 Size: 532 1942 5423 Fragments arranged by size: 5423 1942 532 PflMI CCAn_nnn'nTGG Cuts at: 1108 2744 1108 Size: 1636 6261 PfoI T'CCnGG_A Cuts at: 3332 3332 Size: 7897 PmeI GTTT'AAAC Cuts at: 5201 5201 Size: 7897 PmlI CAC'GTG Cuts at: 2199 2199 Size: 7897 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3675 3707 5966 5998 7479 7511 3675 Size: 32 2259 32 1481 32 4061 Fragments arranged by size: 4061 2259 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2424 2424 Size: 7897 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7897 PsiI TTA'TAA Cuts at: 5467 5467 Size: 7897 PspOMI G'GGCC_C Cuts at: 3172 3382 3172 Size: 210 7687 PstI C_TGCA'G Cuts at: 830 2897 3494 4078 5249 830 Size: 2067 597 584 1171 3478 Fragments arranged by size: 3478 2067 1171 597 584 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7897 PvuII CAG'CTG Cuts at: 4164 4460 4164 Size: 296 7601 SacI G_AGCT'C Cuts at: 2263 2263 Size: 7897 SalI G'TCGA_C Cuts at: 1936 5239 1936 Size: 3303 4594 SanDI GG'GwC_CC Cuts at: 2424 2424 Size: 7897 SbfI CC_TGCA'GG Cuts at: 5249 5249 Size: 7897 ScaI AGT'ACT Cuts at: 6145 7857 6145 Size: 1712 6185 SfcI C'TryA_G Cuts at: 826 1045 2893 3490 4074 5245 6380 7061 7252 Size: 219 1848 597 584 1171 1135 681 191 Cuts at: 7252 826 Size: 1471 Fragments arranged by size: 1848 1471 1171 1135 681 597 584 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7897 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7897 SmaI CCC'GGG Cuts at: 1872 5224 1872 Size: 3352 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4371 4932 6001 6902 7164 820 Size: 197 926 2428 561 1069 901 262 1553 Fragments arranged by size: 2428 1553 1069 926 901 561 262 197 SnaBI TAC'GTA Cuts at: 493 5688 493 Size: 5195 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7897 SphI G_CATG'C Cuts at: 1132 4570 5083 5182 5255 1132 Size: 3438 513 99 73 3774 Fragments arranged by size: 3774 3438 513 99 73 SspI AAT'ATT Cuts at: 5 52 5821 5 Size: 47 5769 2081 Fragments arranged by size: 5769 2081 47 StuI AGG'CCT Cuts at: 1517 3428 1517 Size: 1911 5986 StyI C'CwwG_G Cuts at: 513 1065 2037 2265 3985 5336 513 Size: 552 972 228 1720 1351 3074 Fragments arranged by size: 3074 1720 1351 972 552 228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5924 5941 6094 6279 6724 325 Size: 1255 4344 17 153 185 445 1498 Fragments arranged by size: 4344 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2720 3290 4166 Size: 274 80 33 51 1443 743 570 876 Cuts at: 4166 4424 5129 6143 7855 96 Size: 258 705 1014 1712 138 Fragments arranged by size: 1712 1443 1014 876 743 705 570 274 258 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7857 7889 7857 Size: 32 7865 Tth111I GACn'n_nGTC Cuts at: 1316 4385 1316 Size: 3069 4828 XbaI T'CTAG_A Cuts at: 3775 5233 3775 Size: 1458 6439 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3176 3386 3515 4349 3176 Size: 210 129 834 6724 Fragments arranged by size: 6724 834 210 129 XhoI C'TCGA_G Cuts at: 1943 4371 1943 Size: 2428 5469 XmaI C'CCGG_G Cuts at: 1870 5222 1870 Size: 3352 4545 XmnI GAAnn'nnTTC Cuts at: 3690 6026 3690 Size: 2336 5561 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5705 276 Size: 53 83 186 936 4171 2468 Fragments arranged by size: 4171 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI