(Circular) (Six-base) MAPSORT of: pFN21AB7303.seq Check: 3529 from: 1 to: 8569 pFN21AB7303 8569 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2602 2785 3750 4497 2602 Size: 183 965 747 6674 Fragments arranged by size: 6674 965 747 183 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2067 5705 6379 278 Size: 53 83 186 936 531 3638 674 2468 Fragments arranged by size: 3638 2468 936 674 531 186 83 53 AccI GT'mk_AC Cuts at: 1937 5912 1937 Size: 3975 4594 Acc65I G'GTAC_C Cuts at: 2051 5466 5890 2051 Size: 3415 424 4730 Fragments arranged by size: 4730 3415 424 AclI AA'CG_TT Cuts at: 1563 6696 7069 1563 Size: 5133 373 3063 Fragments arranged by size: 5133 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2827 3073 5026 6632 8016 8284 1632 Size: 1195 246 1953 1606 1384 268 1917 Fragments arranged by size: 1953 1917 1606 1384 1195 268 246 AfeI AGC'GCT Cuts at: 2744 2744 Size: 8569 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8372 AflIII A'CryG_T Cuts at: 1203 2306 2419 2589 2649 4902 7468 8445 1203 Size: 1103 113 170 60 2253 2566 977 1327 Fragments arranged by size: 2566 2253 1327 1103 977 170 113 60 AgeI A'CCGG_T Cuts at: 4513 7400 4513 Size: 2887 5682 AhdI GACnn_n'nnGTC Cuts at: 4762 7298 4762 Size: 2536 6033 AleI CACnn'nnGTG Cuts at: 1166 2249 2648 2966 4383 4514 1166 Size: 1083 399 318 1417 131 5221 Fragments arranged by size: 5221 1417 1083 399 318 131 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2207 3510 4401 4505 5426 1184 Size: 1023 1303 891 104 921 4327 Fragments arranged by size: 4327 1303 1023 921 891 104 AlwNI CAG_nnn'CTG Cuts at: 2209 3655 4423 6312 7884 2209 Size: 1446 768 1889 1572 2894 Fragments arranged by size: 2894 1889 1572 1446 768 ApaI G_GGCC'C Cuts at: 2518 2599 2873 3117 3167 3330 3511 4077 4196 Size: 81 274 244 50 163 181 566 119 Cuts at: 4196 4338 5857 2518 Size: 142 1519 5230 Fragments arranged by size: 5230 1519 566 274 244 181 163 142 119 81 50 ApaLI G'TGCA_C Cuts at: 3414 3527 4376 5411 6626 7782 3414 Size: 113 849 1035 1215 1156 4201 Fragments arranged by size: 4201 1215 1156 1035 849 113 ApoI r'AATT_y Cuts at: 1493 2987 5878 6104 6256 8365 1493 Size: 1494 2891 226 152 2109 1697 Fragments arranged by size: 2891 2109 1697 1494 226 152 AseI AT'TA_AT Cuts at: 160 1032 7123 160 Size: 872 6091 1606 Fragments arranged by size: 6091 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8569 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2196 2547 2628 2694 2866 3116 Size: 73 72 181 351 81 66 172 250 Cuts at: 3116 3504 3941 3975 4935 5190 5216 5506 5720 Size: 388 437 34 960 255 26 290 214 Cuts at: 5720 5822 5894 1870 Size: 102 72 4545 Fragments arranged by size: 4545 960 437 388 351 290 255 250 214 181 172 102 81 73 72 72 66 34 26 BamHI G'GATC_C Cuts at: 5899 6266 5899 Size: 367 8202 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2243 2336 2447 2827 Size: 325 217 627 264 192 93 111 380 Cuts at: 2827 3917 4385 4566 4637 4711 5167 5466 5639 Size: 1090 468 181 71 74 456 299 173 Cuts at: 5639 5890 7345 618 Size: 251 1455 1842 Fragments arranged by size: 1842 1455 1090 627 468 456 380 325 299 264 251 217 192 181 173 111 93 74 71 BanII G_rGCy'C Cuts at: 1391 2518 2599 2873 3117 3133 3167 3330 3511 Size: 1127 81 274 244 16 34 163 181 Cuts at: 3511 4077 4196 4338 4824 5507 5622 5857 5888 Size: 566 119 142 486 683 115 235 31 Cuts at: 5888 1391 Size: 4072 Fragments arranged by size: 4072 1127 683 566 486 274 244 235 181 163 142 119 115 81 34 31 16 BbeI G_GCGC'C Cuts at: 2831 4715 5171 5643 2831 Size: 1884 456 472 5757 Fragments arranged by size: 5757 1884 472 456 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2992 3926 4669 4971 928 Size: 971 1093 934 743 302 4526 Fragments arranged by size: 4526 1093 971 934 743 302 BbvCI CC'TCA_GC Cuts at: 3445 3445 Size: 8569 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6760 6794 1903 Size: 34 4823 34 3678 Fragments arranged by size: 4823 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6461 7671 6461 Size: 1210 7359 BclI T'GATC_A Cuts at: 1548 1548 Size: 8569 BfrBI ATG'CAT Cuts at: 3491 3491 Size: 8569 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2019 2877 3287 Size: 107 122 71 631 605 347 858 410 Cuts at: 3287 3392 4709 4784 5288 5617 5861 7180 136 Size: 105 1317 75 504 329 244 1319 1525 Fragments arranged by size: 1525 1319 1317 858 631 605 504 410 347 329 244 122 107 105 75 71 BglII A'GATC_T Cuts at: 8564 8564 Size: 8569 BlpI GC'TnA_GC Cuts at: 3347 3656 4251 4884 5986 3347 Size: 309 595 633 1102 5930 Fragments arranged by size: 5930 1102 633 595 309 Bme1580I G_kGCm'C Cuts at: 1790 2081 2091 2246 2376 2450 2518 2599 2703 Size: 291 10 155 130 74 68 81 104 Cuts at: 2703 2873 3117 3167 3330 3418 3511 3531 3758 Size: 170 244 50 163 88 93 20 227 Cuts at: 3758 3920 4062 4077 4196 4327 4338 4380 4569 Size: 162 142 15 119 131 11 42 189 Cuts at: 4569 4780 5415 5857 6630 7786 1790 Size: 211 635 442 773 1156 2573 Fragments arranged by size: 2573 1156 773 635 442 291 244 227 211 189 170 163 162 155 142 131 130 119 104 93 88 81 74 68 50 42 20 15 11 10 BmgBI CAC'GTC Cuts at: 1318 2958 2998 3583 4450 4846 5250 1318 Size: 1640 40 585 867 396 404 4637 Fragments arranged by size: 4637 1640 867 585 404 396 40 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2068 3988 4464 Size: 416 53 83 399 24 651 1920 476 Cuts at: 4464 5061 6300 7258 442 Size: 597 1239 958 1753 Fragments arranged by size: 1920 1753 1239 958 651 597 476 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8569 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4975 4975 Size: 8569 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2200 2291 2426 2552 2836 3014 3305 Size: 950 11 91 135 126 284 178 291 Cuts at: 3305 3374 3686 4970 5477 7229 1239 Size: 69 312 1284 507 1752 2579 Fragments arranged by size: 2579 1752 1284 950 507 312 291 284 178 135 126 91 69 11 Bpu10I CC'TnA_GC Cuts at: 3445 3879 3445 Size: 434 8135 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2768 6694 7559 7857 2768 Size: 3926 865 298 3480 Fragments arranged by size: 3926 3480 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2313 3623 4016 7232 882 Size: 617 814 1310 393 3216 2219 Fragments arranged by size: 3216 2219 1310 814 617 393 BsaAI yAC'GTr Cuts at: 493 1204 2092 2309 2592 2650 2749 6360 493 Size: 711 888 217 283 58 99 3611 2702 Fragments arranged by size: 3611 2702 888 711 283 217 99 58 BsaBI GATnn'nnATC Cuts at: 3186 8563 3186 Size: 5377 3192 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 2456 2718 2828 Size: 53 83 186 936 531 392 262 110 Cuts at: 2828 3021 4674 4712 5168 5541 5640 5702 6376 Size: 193 1653 38 456 373 99 62 674 Cuts at: 6376 6758 7393 275 Size: 382 635 1451 Fragments arranged by size: 1653 1451 936 674 635 531 456 392 382 373 262 193 186 110 99 83 62 53 38 BsaWI w'CCGG_w Cuts at: 4513 7002 7400 7674 7821 8232 4513 Size: 2489 398 274 147 411 4850 Fragments arranged by size: 4850 2489 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3659 3689 3955 3985 5450 Size: 30 165 30 2256 30 266 30 1465 Cuts at: 5450 5480 1178 Size: 30 4267 Fragments arranged by size: 4267 2256 1465 266 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3371 3683 4700 5582 1186 Size: 2185 312 1017 882 4173 Fragments arranged by size: 4173 2185 1017 882 312 BseYI C'CCAG_C Cuts at: 2598 2635 2981 3793 4048 4197 5387 7772 8268 Size: 37 346 812 255 149 1190 2385 496 Cuts at: 8268 2598 Size: 2899 Fragments arranged by size: 2899 2385 1190 812 496 346 255 149 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2312 2414 2513 2771 2813 3659 3722 Size: 573 576 102 99 258 42 846 63 Cuts at: 3722 4424 4736 4871 5024 1163 Size: 702 312 135 153 4708 Fragments arranged by size: 4708 846 702 576 573 312 258 153 135 102 99 63 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2044 2877 3887 3925 5677 6019 Size: 275 186 43 833 1010 38 1752 342 Cuts at: 6019 6780 7808 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1752 1028 1010 833 761 342 275 186 43 38 BsiHKAI G_wGCw'C Cuts at: 2577 2865 2997 3418 3531 3769 4380 4824 5247 Size: 288 132 421 113 238 611 444 423 Cuts at: 5247 5415 5829 6630 6715 7786 2577 Size: 168 414 801 85 1071 3360 Fragments arranged by size: 3360 1071 801 611 444 423 421 414 288 238 168 132 113 85 BsmI GAATG_Cn' Cuts at: 6080 6173 6080 Size: 93 8476 BsmBI CGTCTCn'nnnn_ Cuts at: 4391 5351 4391 Size: 960 7609 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2091 2246 2376 2450 2518 2577 Size: 399 291 10 155 130 74 68 59 Cuts at: 2577 2599 2703 2865 2873 2997 3117 3133 3167 Size: 22 104 162 8 124 120 16 34 Cuts at: 3167 3330 3418 3511 3531 3758 3769 3920 4062 Size: 163 88 93 20 227 11 151 142 Cuts at: 4062 4077 4196 4327 4338 4380 4569 4780 4824 Size: 15 119 131 11 42 189 211 44 Cuts at: 4824 5247 5415 5507 5622 5829 5857 5888 6630 Size: 423 168 92 115 207 28 31 742 Cuts at: 6630 6715 7786 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 423 399 291 227 211 207 189 168 163 162 155 151 142 131 130 124 120 119 115 104 93 92 88 85 74 68 59 44 42 34 31 28 22 20 16 15 11 11 10 8 BspEI T'CCGG_A Cuts at: 8232 8232 Size: 8569 BspHI T'CATG_A Cuts at: 4239 6458 8188 4239 Size: 2219 1730 4620 Fragments arranged by size: 4620 2219 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2331 2490 2602 2785 3750 4044 4497 4758 Size: 1487 159 112 183 965 294 453 261 Cuts at: 4758 5664 5924 844 Size: 906 260 3489 Fragments arranged by size: 3489 1487 965 906 453 294 261 260 183 159 112 BsrBI CCG'CTC Cuts at: 1394 1823 2275 2287 3807 3847 3883 6456 1394 Size: 429 452 12 1520 40 36 2573 3507 Fragments arranged by size: 3507 2573 1520 452 429 40 36 12 BsrDI GCAATG_nn' Cuts at: 65 1248 4245 7064 7238 65 Size: 1183 2997 2819 174 1396 Fragments arranged by size: 2997 2819 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2180 2876 3402 3886 3920 4513 5463 Size: 110 370 696 526 484 34 593 950 Cuts at: 5463 7213 7400 1700 Size: 1750 187 2869 Fragments arranged by size: 2869 1750 950 696 593 526 484 370 187 110 34 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8569 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8569 BssSI C'ACGA_G Cuts at: 6629 7641 6629 Size: 1012 7557 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8569 BstBI TT'CG_AA Cuts at: 7373 7373 Size: 8569 BstEII G'GTnAC_C Cuts at: 1184 4135 5707 1184 Size: 2951 1572 4046 Fragments arranged by size: 4046 2951 1572 BstXI CCAn_nnnn'nTGG Cuts at: 2348 2677 4111 2348 Size: 329 1434 6806 Fragments arranged by size: 6806 1434 329 BstYI r'GATC_y Cuts at: 1972 5451 5899 6266 6651 6668 8120 8206 8218 Size: 3479 448 367 385 17 1452 86 12 Cuts at: 8218 8564 1972 Size: 346 1977 Fragments arranged by size: 3479 1977 1452 448 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2382 3264 2382 Size: 882 7687 BtgI C'CryG_G Cuts at: 513 1065 2004 2044 2347 2434 2676 2764 2832 Size: 552 939 40 303 87 242 88 68 Cuts at: 2832 2961 3057 3366 4069 4164 5084 5623 5644 Size: 129 96 309 703 95 920 539 21 Cuts at: 5644 5865 513 Size: 221 3217 Fragments arranged by size: 3217 939 920 703 552 539 309 303 242 221 129 96 95 88 87 68 40 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3484 487 Size: 683 56 2258 5572 Fragments arranged by size: 5572 2258 683 56 BtsI GCAGTG_nn' Cuts at: 2264 3680 3934 6085 6879 6899 2264 Size: 1416 254 2151 794 20 3934 Fragments arranged by size: 3934 2151 1416 794 254 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8569 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8534 DraI TTT'AAA Cuts at: 5873 5873 Size: 8569 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7576 809 Size: 507 6260 1802 Fragments arranged by size: 6260 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 2367 2874 Size: 54 1425 50 275 18 423 114 507 Cuts at: 2874 2923 3294 3363 3384 3884 3922 4523 4554 Size: 49 371 69 21 500 38 601 31 Cuts at: 4554 4890 5609 5674 6016 6905 8534 8 Size: 336 719 65 342 889 1629 43 Fragments arranged by size: 1629 1425 889 719 601 507 500 423 371 342 336 275 114 69 65 54 50 49 43 38 31 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2874 3884 3922 5674 6016 1537 Size: 275 1062 1010 38 1752 342 4090 Fragments arranged by size: 4090 1752 1062 1010 342 275 38 EarI CTCTTCn'nnn_ Cuts at: 1353 2391 3089 6499 1353 Size: 1038 698 3410 3423 Fragments arranged by size: 3423 3410 1038 698 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2147 4982 5092 7156 7530 7676 Size: 1064 138 558 2835 110 2064 374 146 Cuts at: 7676 387 Size: 1280 Fragments arranged by size: 2835 2064 1280 1064 558 374 146 138 110 EcoICRI GAG'CTC Cuts at: 4822 4822 Size: 8569 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2200 2291 2426 2552 2827 2836 Size: 393 557 11 91 135 126 275 9 Cuts at: 2836 3014 3073 3305 3374 3686 4970 5026 5477 Size: 178 59 232 69 312 1284 56 451 Cuts at: 5477 6632 7229 8016 8284 1239 Size: 1155 597 787 268 1524 Fragments arranged by size: 1524 1284 1155 787 597 557 451 393 312 275 268 232 178 135 126 91 69 59 56 11 9 EcoNI CCTnn'n_nnAGG Cuts at: 2171 2463 3356 5018 2171 Size: 292 893 1662 5722 Fragments arranged by size: 5722 1662 893 292 EcoO109I rG'GnC_Cy Cuts at: 2119 2353 2479 2514 2515 3034 3140 3163 3164 Size: 234 126 35 1 519 106 23 1 Cuts at: 3164 3327 3508 3711 4074 4193 4702 4723 4939 Size: 163 181 203 363 119 509 21 216 Cuts at: 4939 5280 5289 5384 5767 5776 2119 Size: 341 9 95 383 9 4912 Fragments arranged by size: 4912 519 509 383 363 341 234 216 203 181 163 126 119 106 95 35 23 21 9 9 1 1 EcoRI G'AATT_C Cuts at: 2987 5878 6256 2987 Size: 2891 378 5300 Fragments arranged by size: 5300 2891 378 FspI TGC'GCA Cuts at: 2684 7075 2684 Size: 4391 4178 FspAI rTGC'GCAy Cuts at: 2684 2684 Size: 8569 HaeII r_GCGC'y Cuts at: 1678 2297 2746 2831 4715 5031 5171 5576 5599 Size: 619 449 85 1884 316 140 405 23 Cuts at: 5599 5643 7716 1678 Size: 44 2073 2531 Fragments arranged by size: 2531 2073 1884 619 449 405 316 140 85 44 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3955 3987 4450 4482 4748 Size: 32 135 32 2383 32 463 32 266 Cuts at: 4748 4780 5184 5216 5671 5703 7210 7242 7284 Size: 32 404 32 455 32 1507 32 42 Cuts at: 7284 7316 1373 Size: 32 2626 Fragments arranged by size: 2626 2383 1507 463 455 404 266 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3307 5913 6159 669 Size: 988 281 1369 2606 246 3079 Fragments arranged by size: 3079 2606 1369 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8569 HpaI GTT'AAC Cuts at: 6159 6159 Size: 8569 KasI G'GCGC_C Cuts at: 2827 4711 5167 5639 2827 Size: 1884 456 472 5757 Fragments arranged by size: 5757 1884 472 456 KpnI G_GTAC'C Cuts at: 2055 5470 5894 2055 Size: 3415 424 4730 Fragments arranged by size: 4730 3415 424 MfeI C'AATT_G Cuts at: 6168 6168 Size: 8569 MluI A'CGCG_T Cuts at: 2419 8445 2419 Size: 6026 2543 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4746 7683 7867 1305 Size: 3441 2937 184 2007 Fragments arranged by size: 3441 2937 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2255 2925 3296 3365 3386 Size: 54 1425 343 423 670 371 69 21 Cuts at: 3386 4556 10 Size: 1170 4023 Fragments arranged by size: 4023 1425 1170 670 423 371 343 69 54 21 MslI CAynn'nnrTG Cuts at: 518 1166 2237 2249 2648 2675 2966 3492 4109 Size: 648 1071 12 399 27 291 526 617 Cuts at: 4109 4163 4373 4383 4470 4514 6527 6886 7045 Size: 54 210 10 87 44 2013 359 159 Cuts at: 7045 518 Size: 2042 Fragments arranged by size: 2042 2013 1071 648 617 526 399 359 291 210 159 87 54 44 27 12 10 MspA1I CmG'CkG Cuts at: 1606 2209 2847 3017 3043 3065 3346 3643 4201 Size: 603 638 170 26 22 281 297 558 Cuts at: 4201 4216 4737 4810 5046 5086 5391 5406 5646 Size: 15 521 73 236 40 305 15 240 Cuts at: 5646 5985 6662 7810 8055 1606 Size: 339 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 638 603 558 521 339 305 297 281 245 240 236 170 73 40 26 22 15 15 NaeI GCC'GGC Cuts at: 2182 2878 3404 2182 Size: 696 526 7347 Fragments arranged by size: 7347 696 526 NarI GG'CG_CC Cuts at: 2828 4712 5168 5640 2828 Size: 1884 456 472 5757 Fragments arranged by size: 5757 1884 472 456 NcoI C'CATG_G Cuts at: 513 1065 2004 2676 2832 3366 4069 513 Size: 552 939 672 156 534 703 5013 Fragments arranged by size: 5013 939 703 672 552 534 156 NdeI CA'TA_TG Cuts at: 387 387 Size: 8569 NgoMIV G'CCGG_C Cuts at: 2180 2876 3402 2180 Size: 696 526 7347 Fragments arranged by size: 7347 696 526 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8569 NotI GC'GGCC_GC Cuts at: 6016 6016 Size: 8569 NsiI A_TGCA'T Cuts at: 3493 3493 Size: 8569 NspI r_CATG'y Cuts at: 1132 1229 2188 3491 3495 4906 5927 7472 1132 Size: 97 959 1303 4 1411 1021 1545 2229 Fragments arranged by size: 2229 1545 1411 1303 1021 959 97 4 PasI CC'CwG_GG Cuts at: 1348 2081 3511 3562 3697 5305 5329 1348 Size: 733 1430 51 135 1608 24 4588 Fragments arranged by size: 4588 1608 1430 733 135 51 24 PciI A'CATG_T Cuts at: 4902 7468 4902 Size: 2566 6003 PflMI CCAn_nnn'nTGG Cuts at: 1108 2209 3457 4813 1108 Size: 1101 1248 1356 4864 Fragments arranged by size: 4864 1356 1248 1101 PfoI T'CCnGG_A Cuts at: 2166 4680 5202 5454 2166 Size: 2514 522 252 5281 Fragments arranged by size: 5281 2514 522 252 PmeI GTTT'AAAC Cuts at: 5873 5873 Size: 8569 PmlI CAC'GTG Cuts at: 2092 2309 2592 2650 2092 Size: 217 283 58 8011 Fragments arranged by size: 8011 283 217 58 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6638 6670 8151 8183 6638 Size: 32 1481 32 7024 Fragments arranged by size: 7024 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2119 2353 2479 3140 3711 4723 5384 5767 5776 Size: 234 126 661 571 1012 661 383 9 Cuts at: 5776 2119 Size: 4912 Fragments arranged by size: 4912 1012 661 661 571 383 234 126 9 PshAI GACnn'nnGTC Cuts at: 1613 2217 1613 Size: 604 7965 PsiI TTA'TAA Cuts at: 6139 6139 Size: 8569 PspOMI G'GGCC_C Cuts at: 2514 2595 2869 3113 3163 3326 3507 4073 4192 Size: 81 274 244 50 163 181 566 119 Cuts at: 4192 4334 5853 2514 Size: 142 1519 5230 Fragments arranged by size: 5230 1519 566 274 244 181 163 142 119 81 50 PspXI vC'TCGA_Gb Cuts at: 3941 3941 Size: 8569 PstI C_TGCA'G Cuts at: 830 3693 4275 4736 5921 830 Size: 2863 582 461 1185 3478 Fragments arranged by size: 3478 2863 1185 582 461 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8569 PvuII CAG'CTG Cuts at: 3017 3043 3643 4201 4216 4810 5391 3017 Size: 26 600 558 15 594 581 6195 Fragments arranged by size: 6195 600 594 581 558 26 15 SacI G_AGCT'C Cuts at: 4824 4824 Size: 8569 SacII CC_GC'GG Cuts at: 5087 5647 5087 Size: 560 8009 SalI G'TCGA_C Cuts at: 1936 5911 1936 Size: 3975 4594 SanDI GG'GwC_CC Cuts at: 5384 5767 5384 Size: 383 8186 SbfI CC_TGCA'GG Cuts at: 5921 5921 Size: 8569 ScaI AGT'ACT Cuts at: 6817 8529 6817 Size: 1712 6857 SfcI C'TryA_G Cuts at: 826 1045 3689 4271 4732 5917 7052 7733 7924 Size: 219 2644 582 461 1185 1135 681 191 Cuts at: 7924 826 Size: 1471 Fragments arranged by size: 2644 1471 1185 1135 681 582 461 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3392 5288 5861 3392 Size: 1896 573 6100 Fragments arranged by size: 6100 1896 573 SfoI GGC'GCC Cuts at: 2829 4713 5169 5641 2829 Size: 1884 456 472 5757 Fragments arranged by size: 5757 1884 472 456 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8569 SgrAI Cr'CCGG_yG Cuts at: 1700 4513 1700 Size: 2813 5756 SmaI CCC'GGG Cuts at: 1872 2198 2630 3506 3977 4937 5722 5896 1872 Size: 326 432 876 471 960 785 174 4545 Fragments arranged by size: 4545 960 876 785 471 432 326 174 SmlI C'TyrA_G Cuts at: 820 1017 1943 2547 2783 3941 6673 7574 7836 Size: 197 926 604 236 1158 2732 901 262 Cuts at: 7836 820 Size: 1553 Fragments arranged by size: 2732 1553 1158 926 901 604 262 236 197 SnaBI TAC'GTA Cuts at: 493 6360 493 Size: 5867 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8569 SphI G_CATG'C Cuts at: 1132 2188 3491 5927 1132 Size: 1056 1303 2436 3774 Fragments arranged by size: 3774 2436 1303 1056 SrfI GCCC'GGGC Cuts at: 3506 3506 Size: 8569 SspI AAT'ATT Cuts at: 5 52 6493 5 Size: 47 6441 2081 Fragments arranged by size: 6441 2081 47 StuI AGG'CCT Cuts at: 1517 3269 4856 1517 Size: 1752 1587 5230 Fragments arranged by size: 5230 1752 1587 StyI C'CwwG_G Cuts at: 513 1065 2004 2676 2832 3366 4069 6008 513 Size: 552 939 672 156 534 703 1939 3074 Fragments arranged by size: 3074 1939 939 703 672 552 534 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2354 2606 3150 3807 4373 5606 6596 Size: 1255 774 252 544 657 566 1233 990 Cuts at: 6596 6613 6766 6951 7396 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1233 990 774 657 566 544 445 252 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6815 8527 96 Size: 274 80 33 51 1443 4838 1712 138 Fragments arranged by size: 4838 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2298 2330 3659 3691 8529 8561 2298 Size: 32 1329 32 4838 32 2306 Fragments arranged by size: 4838 2306 1329 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5540 1316 Size: 4224 4345 XbaI T'CTAG_A Cuts at: 5905 5905 Size: 8569 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2236 2266 2918 3542 2236 Size: 30 652 624 7263 Fragments arranged by size: 7263 652 624 30 XhoI C'TCGA_G Cuts at: 1943 2547 3941 1943 Size: 604 1394 6571 Fragments arranged by size: 6571 1394 604 XmaI C'CCGG_G Cuts at: 1870 2196 2628 3504 3975 4935 5720 5894 1870 Size: 326 432 876 471 960 785 174 4545 Fragments arranged by size: 4545 960 876 785 471 432 326 174 XmnI GAAnn'nnTTC Cuts at: 6698 6698 Size: 8569 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2065 5703 6377 276 Size: 53 83 186 936 531 3638 674 2468 Fragments arranged by size: 3638 2468 936 674 531 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BsiWI BstZ17I DraIII EcoRV FalI FseI NruI PacI PsrI RsrII SapI SexAI SwaI