(Circular) (Six-base) MAPSORT of: pFN21AB7301.seq Check: 8307 from: 1 to: 7258 pFN21AB7301 7258 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3260 5068 278 Size: 53 83 186 936 1724 1808 2468 Fragments arranged by size: 2468 1808 1724 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3318 4601 1937 Size: 1381 1283 4594 Fragments arranged by size: 4594 1381 1283 Acc65I G'GTAC_C Cuts at: 4579 4579 Size: 7258 AclI AA'CG_TT Cuts at: 1563 5385 5758 1563 Size: 3822 373 3063 Fragments arranged by size: 3822 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2784 2964 3147 3252 3317 3984 4044 5321 Size: 1152 180 183 105 65 667 60 1277 Cuts at: 5321 6705 6973 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1277 1152 667 268 183 180 105 65 60 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7061 AflIII A'CryG_T Cuts at: 1203 3044 6157 7134 1203 Size: 1841 3113 977 1327 Fragments arranged by size: 3113 1841 1327 977 AgeI A'CCGG_T Cuts at: 6089 6089 Size: 7258 AhdI GACnn_n'nnGTC Cuts at: 3256 5987 3256 Size: 2731 4527 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7258 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7258 AlwNI CAG_nnn'CTG Cuts at: 2234 4204 5001 6573 2234 Size: 1970 797 1572 2919 Fragments arranged by size: 2919 1970 1572 797 ApaLI G'TGCA_C Cuts at: 5315 6471 5315 Size: 1156 6102 ApoI r'AATT_y Cuts at: 1493 2438 2483 3167 3348 3374 3466 4567 4793 Size: 945 45 684 181 26 92 1101 226 Cuts at: 4793 4945 7054 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1101 945 684 226 181 152 92 45 26 AseI AT'TA_AT Cuts at: 160 1032 4247 5812 160 Size: 872 3215 1565 1606 Fragments arranged by size: 3215 1606 1565 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7258 AvaI C'yCGr_G Cuts at: 1870 1943 2062 4583 1870 Size: 73 119 2521 4545 Fragments arranged by size: 4545 2521 119 73 BamHI G'GATC_C Cuts at: 4588 4955 4588 Size: 367 6891 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2175 2576 4579 6034 618 Size: 325 217 627 388 401 2003 1455 1842 Fragments arranged by size: 2003 1842 1455 627 401 388 325 217 BanII G_rGCy'C Cuts at: 1391 4577 1391 Size: 3186 4072 BbeI G_GCGC'C Cuts at: 2179 2179 Size: 7258 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6287 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5449 5483 1903 Size: 34 3512 34 3678 Fragments arranged by size: 3678 3512 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5150 6360 5150 Size: 1210 6048 BclI T'GATC_A Cuts at: 1548 3547 1548 Size: 1999 5259 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5869 136 Size: 107 122 71 631 605 4197 1525 Fragments arranged by size: 4197 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3400 7253 3400 Size: 3853 3405 BlpI GC'TnA_GC Cuts at: 4675 4675 Size: 7258 Bme1580I G_kGCm'C Cuts at: 1790 5319 6475 1790 Size: 3529 1156 2573 Fragments arranged by size: 3529 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3047 1318 Size: 1729 5529 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3082 4989 5947 Size: 416 53 83 399 24 1665 1907 958 Cuts at: 5947 442 Size: 1753 Fragments arranged by size: 1907 1753 1665 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7258 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2741 4071 5918 1239 Size: 1502 1330 1847 2579 Fragments arranged by size: 2579 1847 1502 1330 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4394 5383 6248 6546 4394 Size: 989 865 298 5106 Fragments arranged by size: 5106 989 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5921 882 Size: 617 4422 2219 Fragments arranged by size: 4422 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5049 493 Size: 711 3845 2702 Fragments arranged by size: 3845 2702 711 BsaBI GATnn'nnATC Cuts at: 2241 7252 2241 Size: 5011 2247 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2176 3257 5065 5447 Size: 53 83 186 936 643 1081 1808 382 Cuts at: 5447 6082 275 Size: 635 1451 Fragments arranged by size: 1808 1451 1081 936 643 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3331 5691 6089 6363 6510 6921 3331 Size: 2360 398 274 147 411 3668 Fragments arranged by size: 3668 2360 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3672 3702 1178 Size: 30 165 30 2269 30 4734 Fragments arranged by size: 4734 2269 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2849 2867 3015 3662 3674 4391 1186 Size: 1663 18 148 647 12 717 4053 Fragments arranged by size: 4053 1663 717 647 148 18 12 BseYI C'CCAG_C Cuts at: 6461 6957 6461 Size: 496 6762 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3867 1163 Size: 573 2131 4554 Fragments arranged by size: 4554 2131 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4708 5469 6497 1540 Size: 275 186 2707 761 1028 2301 Fragments arranged by size: 2707 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4197 5319 5404 6475 4197 Size: 1122 85 1071 4980 Fragments arranged by size: 4980 1122 1071 85 BsmI GAATG_Cn' Cuts at: 4769 4862 4769 Size: 93 7165 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4197 4577 5319 5404 6475 1391 Size: 399 2407 380 742 85 1071 2174 Fragments arranged by size: 2407 2174 1071 742 399 380 85 BspEI T'CCGG_A Cuts at: 3331 6921 3331 Size: 3590 3668 BspHI T'CATG_A Cuts at: 2131 3550 4230 5147 6877 2131 Size: 1419 680 917 1730 2512 Fragments arranged by size: 2512 1730 1419 917 680 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3754 4613 844 Size: 2910 859 3489 Fragments arranged by size: 3489 2910 859 BsrBI CCG'CTC Cuts at: 1394 1823 5145 1394 Size: 429 3322 3507 Fragments arranged by size: 3507 3322 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3513 4302 5753 5927 65 Size: 1183 2265 789 1451 174 1396 Fragments arranged by size: 2265 1451 1396 1183 789 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5902 6089 1700 Size: 110 4092 187 2869 Fragments arranged by size: 4092 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4299 96 Size: 4203 3055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7258 BssSI C'ACGA_G Cuts at: 3027 5318 6330 3027 Size: 2291 1012 3955 Fragments arranged by size: 3955 2291 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3760 794 Size: 2966 4292 BstBI TT'CG_AA Cuts at: 6062 6062 Size: 7258 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7258 BstYI r'GATC_y Cuts at: 1972 3400 4333 4588 4955 5340 5357 6809 6895 Size: 1428 933 255 367 385 17 1452 86 Cuts at: 6895 6907 7253 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1428 933 385 367 346 255 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3444 3444 Size: 7258 BtgI C'CryG_G Cuts at: 513 1065 2160 513 Size: 552 1095 5611 Fragments arranged by size: 5611 1095 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6519 Fragments arranged by size: 6519 683 56 BtsI GCAGTG_nn' Cuts at: 4774 5568 5588 4774 Size: 794 20 6444 Fragments arranged by size: 6444 794 20 ClaI AT'CG_AT Cuts at: 1554 2319 1554 Size: 765 6493 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7223 DraI TTT'AAA Cuts at: 3121 3583 4562 3121 Size: 462 979 5817 Fragments arranged by size: 5817 979 462 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6265 809 Size: 507 4949 1802 Fragments arranged by size: 4949 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4705 5594 7223 Size: 54 1425 50 275 18 2875 889 1629 Cuts at: 7223 8 Size: 43 Fragments arranged by size: 2875 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4705 1537 Size: 275 2893 4090 Fragments arranged by size: 4090 2893 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2545 2620 2826 2844 3186 3585 3639 3651 Size: 1192 75 206 18 342 399 54 12 Cuts at: 3651 3846 3960 3972 5188 1353 Size: 195 114 12 1216 3423 Fragments arranged by size: 3423 1216 1192 399 342 206 195 114 75 54 18 12 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5845 6219 6365 387 Size: 1064 138 4256 374 146 1280 Fragments arranged by size: 4256 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2741 2784 2964 3147 3252 3317 3984 Size: 393 1109 43 180 183 105 65 667 Cuts at: 3984 4044 4071 5321 5918 6705 6973 1239 Size: 60 27 1250 597 787 268 1524 Fragments arranged by size: 1524 1250 1109 787 667 597 393 268 183 180 105 65 60 43 27 EcoO109I rG'GnC_Cy Cuts at: 2059 2059 Size: 7258 EcoRI G'AATT_C Cuts at: 4567 4945 4567 Size: 378 6880 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4001 4186 4001 Size: 185 7073 FspI TGC'GCA Cuts at: 5764 5764 Size: 7258 HaeII r_GCGC'y Cuts at: 1678 2179 6405 1678 Size: 501 4226 2531 Fragments arranged by size: 4226 2531 501 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2750 2782 3031 3063 3242 Size: 32 135 32 1178 32 249 32 179 Cuts at: 3242 3265 3274 3297 3440 3472 4067 4099 5899 Size: 23 9 23 143 32 595 32 1800 Cuts at: 5899 5931 5973 6005 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1800 1178 595 249 179 143 135 42 32 32 32 32 32 32 32 32 23 23 9 HincII GTy'rAC Cuts at: 669 1657 1938 4276 4602 4848 669 Size: 988 281 2338 326 246 3079 Fragments arranged by size: 3079 2338 988 326 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7258 HpaI GTT'AAC Cuts at: 4276 4848 4276 Size: 572 6686 KasI G'GCGC_C Cuts at: 2175 2175 Size: 7258 KpnI G_GTAC'C Cuts at: 4583 4583 Size: 7258 MfeI C'AATT_G Cuts at: 4857 4857 Size: 7258 MluI A'CGCG_T Cuts at: 7134 7134 Size: 7258 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6372 6556 1305 Size: 5067 184 2007 Fragments arranged by size: 5067 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5436 Fragments arranged by size: 5436 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5216 5575 5734 518 Size: 648 4050 359 159 2042 Fragments arranged by size: 4050 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 4674 5351 6499 6744 1606 Size: 432 2636 677 1148 245 2120 Fragments arranged by size: 2636 2120 1148 677 432 245 NarI GG'CG_CC Cuts at: 2176 2176 Size: 7258 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6706 NdeI CA'TA_TG Cuts at: 387 387 Size: 7258 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7258 NotI GC'GGCC_GC Cuts at: 4705 4705 Size: 7258 NspI r_CATG'y Cuts at: 1132 1229 2999 4616 6161 1132 Size: 97 1770 1617 1545 2229 Fragments arranged by size: 2229 1770 1617 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7258 PciI A'CATG_T Cuts at: 6157 6157 Size: 7258 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7258 PmeI GTTT'AAAC Cuts at: 4562 4562 Size: 7258 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5327 5359 6840 6872 5327 Size: 32 1481 32 5713 Fragments arranged by size: 5713 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2059 2059 Size: 7258 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7258 PsiI TTA'TAA Cuts at: 4828 4828 Size: 7258 PstI C_TGCA'G Cuts at: 830 4610 830 Size: 3780 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7258 SalI G'TCGA_C Cuts at: 1936 4600 1936 Size: 2664 4594 SapI GCTCTTCn'nnn_ Cuts at: 3186 3585 3186 Size: 399 6859 SbfI CC_TGCA'GG Cuts at: 4610 4610 Size: 7258 ScaI AGT'ACT Cuts at: 3302 5506 7218 3302 Size: 2204 1712 3342 Fragments arranged by size: 3342 2204 1712 SfcI C'TryA_G Cuts at: 826 1045 2196 2406 2532 4606 5741 6422 6613 Size: 219 1151 210 126 2074 1135 681 191 Cuts at: 6613 826 Size: 1471 Fragments arranged by size: 2074 1471 1151 1135 681 219 210 191 126 SfoI GGC'GCC Cuts at: 2177 2177 Size: 7258 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7258 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7258 SmaI CCC'GGG Cuts at: 1872 4585 1872 Size: 2713 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4373 5362 6263 6525 820 Size: 197 926 2430 989 901 262 1553 Fragments arranged by size: 2430 1553 989 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5049 493 Size: 4556 2702 SpeI A'CTAG_T Cuts at: 152 2936 152 Size: 2784 4474 SphI G_CATG'C Cuts at: 1132 2999 4616 1132 Size: 1867 1617 3774 Fragments arranged by size: 3774 1867 1617 SspI AAT'ATT Cuts at: 5 52 3141 3491 5182 5 Size: 47 3089 350 1691 2081 Fragments arranged by size: 3089 2081 1691 350 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7258 StyI C'CwwG_G Cuts at: 513 1065 3896 4697 513 Size: 552 2831 801 3074 Fragments arranged by size: 3074 2831 801 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5285 5302 5455 5640 6085 325 Size: 1255 3705 17 153 185 445 1498 Fragments arranged by size: 3705 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3300 4299 5504 Size: 274 80 33 51 1443 1323 999 1205 Cuts at: 5504 7216 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1323 1205 999 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7218 7250 7218 Size: 32 7226 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7258 XbaI T'CTAG_A Cuts at: 3260 4594 3260 Size: 1334 5924 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3249 3249 Size: 7258 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7258 XmaI C'CCGG_G Cuts at: 1870 4583 1870 Size: 2713 4545 XmnI GAAnn'nnTTC Cuts at: 4169 5387 4169 Size: 1218 6040 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3258 5066 276 Size: 53 83 186 936 1724 1808 2468 Fragments arranged by size: 2468 1808 1724 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI