Restriction map for
pFN21AB7301
(Linear) (Six-base) MAPSORT of: KIBB7301.seq Check: 2702 from: 1 to: 2553
KIBB7301.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AatII G_ACGT'C
Cuts at: 0 1255 2553
Size: 1255 1298
AccI GT'mk_AC
Cuts at: 0 1313 2553
Size: 1313 1240
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 959 1142 1247 1312 1979 2039 2553
Size: 779 180 183 105 65 667 60 514
Fragments arranged by size:
779 667 514 183 180 105 65 60
AflIII A'CryG_T
Cuts at: 0 1039 2553
Size: 1039 1514
AhdI GACnn_n'nnGTC
Cuts at: 0 1251 2553
Size: 1251 1302
AlwNI CAG_nnn'CTG
Cuts at: 0 229 2199 2553
Size: 229 1970 354
Fragments arranged by size:
1970 354 229
ApoI r'AATT_y
Cuts at: 0 433 478 1162 1343 1369 1461 2553
Size: 433 45 684 181 26 92 1092
Fragments arranged by size:
1092 684 433 181 92 45 26
AseI AT'TA_AT
Cuts at: 0 2242 2553
Size: 2242 311
AvaI C'yCGr_G
Cuts at: 0 57 2553
Size: 57 2496
BanI G'GyrC_C
Cuts at: 0 170 571 2553
Size: 170 401 1982
Fragments arranged by size:
1982 401 170
BbeI G_GCGC'C
Cuts at: 0 174 2553
Size: 174 2379
BclI T'GATC_A
Cuts at: 0 1542 2553
Size: 1542 1011
BglII A'GATC_T
Cuts at: 0 1395 2553
Size: 1395 1158
BmgBI CAC'GTC
Cuts at: 0 1042 2553
Size: 1042 1511
BmrI ACTGGGnnnn_n'
Cuts at: 0 1077 2553
Size: 1077 1476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 2066 2553
Size: 736 1330 487
Fragments arranged by size:
1330 736 487
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2389 2553
Size: 2389 164
BsaBI GATnn'nnATC
Cuts at: 0 236 2553
Size: 236 2317
BsaHI Gr'CG_yC
Cuts at: 0 171 1252 2553
Size: 171 1081 1301
Fragments arranged by size:
1301 1081 171
BsaWI w'CCGG_w
Cuts at: 0 1326 2553
Size: 1326 1227
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1667 1697 2553
Size: 1667 30 856
Fragments arranged by size:
1667 856 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 844 862 1010 1657 1669 2386 2553
Size: 844 18 148 647 12 717 167
Fragments arranged by size:
844 717 647 167 148 18 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1862 2553
Size: 1862 691
BsiHKAI G_wGCw'C
Cuts at: 0 2192 2553
Size: 2192 361
Bsp1286I G_dGCh'C
Cuts at: 0 2192 2553
Size: 2192 361
BspEI T'CCGG_A
Cuts at: 0 1326 2553
Size: 1326 1227
BspHI T'CATG_A
Cuts at: 0 126 1545 2225 2553
Size: 126 1419 680 328
Fragments arranged by size:
1419 680 328 126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1749 2553
Size: 1749 804
BsrDI GCAATG_nn'
Cuts at: 0 1508 2297 2553
Size: 1508 789 256
Fragments arranged by size:
1508 789 256
BsrGI T'GTAC_A
Cuts at: 0 2294 2553
Size: 2294 259
BssSI C'ACGA_G
Cuts at: 0 1022 2553
Size: 1022 1531
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1755 2553
Size: 1755 798
BstYI r'GATC_y
Cuts at: 0 1395 2328 2553
Size: 1395 933 225
Fragments arranged by size:
1395 933 225
Bsu36I CC'TnA_GG
Cuts at: 0 1439 2553
Size: 1439 1114
BtgI C'CryG_G
Cuts at: 0 155 2553
Size: 155 2398
ClaI AT'CG_AT
Cuts at: 0 314 2553
Size: 314 2239
DraI TTT'AAA
Cuts at: 0 1116 1578 2553
Size: 1116 462 975
Fragments arranged by size:
1116 975 462
EarI CTCTTCn'nnn_
Cuts at: 0 540 615 821 839 1181 1580 1634 1646
Size: 540 75 206 18 342 399 54 12
Cuts at: 1646 1841 1955 1967 2553
Size: 195 114 12 586
Fragments arranged by size:
586 540 399 342 206 195 114 75
54 18 12 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 779 959 1142 1247 1312 1979 2039
Size: 736 43 180 183 105 65 667 60
Cuts at: 2039 2066 2553
Size: 27 487
Fragments arranged by size:
736 667 487 183 180 105 65 60
43 27
EcoO109I rG'GnC_Cy
Cuts at: 0 54 2553
Size: 54 2499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1996 2181 2553
Size: 1996 185 372
Fragments arranged by size:
1996 372 185
HaeII r_GCGC'y
Cuts at: 0 174 2553
Size: 174 2379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 745 777 1026 1058 1237 1260 1269 1292
Size: 745 32 249 32 179 23 9 23
Cuts at: 1292 1435 1467 2062 2094 2553
Size: 143 32 595 32 459
Fragments arranged by size:
745 595 459 249 179 143 32 32
32 32 23 23 9
HincII GTy'rAC
Cuts at: 0 2271 2553
Size: 2271 282
HpaI GTT'AAC
Cuts at: 0 2271 2553
Size: 2271 282
KasI G'GCGC_C
Cuts at: 0 170 2553
Size: 170 2383
MspA1I CmG'CkG
Cuts at: 0 33 2553
Size: 33 2520
NarI GG'CG_CC
Cuts at: 0 171 2553
Size: 171 2382
NspI r_CATG'y
Cuts at: 0 994 2553
Size: 994 1559
PpuMI rG'GwC_Cy
Cuts at: 0 54 2553
Size: 54 2499
SapI GCTCTTCn'nnn_
Cuts at: 0 1181 1580 2553
Size: 1181 399 973
Fragments arranged by size:
1181 973 399
ScaI AGT'ACT
Cuts at: 0 1297 2553
Size: 1297 1256
SfcI C'TryA_G
Cuts at: 0 191 401 527 2553
Size: 191 210 126 2026
Fragments arranged by size:
2026 210 191 126
SfoI GGC'GCC
Cuts at: 0 172 2553
Size: 172 2381
SmlI C'TyrA_G
Cuts at: 0 2368 2553
Size: 2368 185
SpeI A'CTAG_T
Cuts at: 0 931 2553
Size: 931 1622
SphI G_CATG'C
Cuts at: 0 994 2553
Size: 994 1559
SspI AAT'ATT
Cuts at: 0 1136 1486 2553
Size: 1136 350 1067
Fragments arranged by size:
1136 1067 350
StyI C'CwwG_G
Cuts at: 0 1891 2553
Size: 1891 662
TatI w'GTAC_w
Cuts at: 0 1295 2294 2553
Size: 1295 999 259
Fragments arranged by size:
1295 999 259
XbaI T'CTAG_A
Cuts at: 0 1255 2553
Size: 1255 1298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1244 2553
Size: 1244 1309
XmnI GAAnn'nnTTC
Cuts at: 0 2164 2553
Size: 2164 389
ZraI GAC'GTC
Cuts at: 0 1253 2553
Size: 1253 1300
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AlwNI ApoI AseI
AvaI BanI BbeI BclI BglII BmgBI BmrI BpmI
BpuEI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrDI BsrGI BssSI BstAPI
BstYI Bsu36I BtgI ClaI DraI EarI Eco57MI EcoO109I
FalI HaeII Hin4I HincII HpaI KasI MspA1I NarI
NspI PpuMI SapI ScaI SfcI SfoI SmlI SpeI
SphI SspI StyI TatI XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BanII BbsI BbvCI BcgI BciVI BfrBI BglI BlpI
Bme1580I BmtI BplI Bpu10I BsaI BsaAI BseYI BsiEI
BsiWI BsmI BsmBI BsrBI BsrFI BssHII BstBI BstEII
BstXI BstZ17I BtgZI BtsI CspCI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HindIII KpnI MfeI MluI MmeI MscI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SrfI StuI SwaI TaqII
TstI Tth111I XhoI XmaI