Restriction map for
pFN21AB7287
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I ApaLI AscI Asp718I
AspEI AvrII BbeI BbrPI BciVI
BfrI BlnI BlpI Bpu1102I BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraII DraIII Eam1105I
EcoO109I EcoT22I HpaI KasI KpnI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SalI SbfI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 2626 GTCTAC GT^[AC][GT]AC
AcyI 60 GACGCC G[GA]^CG[CT]C
AflIII 716 ACACGT A^C[GA][CT]GT
Asp700I 1719 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1284 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 470 TGCGCA TGC^GCA
BcgI 1320 CGAGCTCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3455 AGATCT A^GATCT
BsaHI 60 GACGCC G[GA]^CG[CT]C
BspHI 2591 TCATGA T^CATGA
BssSI 1117 CTCGTG CACGAG(-5/-1)
BstAPI 99 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2867 CCAGCCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 2083 TTTAAA TTT^AAA
DrdI 3684 GACTTTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2889 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1723 GAATTC G^AATTC
FseI 882 GGCCGGCC GGCCGG^CC
FspI 470 TGCGCA TGC^GCA
HgaI 60 GACGC GACGC(5/10)
Hin1I 60 GACGCC G[GA]^CG[CT]C
HincII 1510 GTTGAC GT[CT]^[GA]AC
HindII 1510 GTTGAC GT[CT]^[GA]AC
KspI 3578 CCGCGG CCGC^GG
PacI 1756 TTAATTAA TTAAT^TAA
PflFI 1284 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2591 TCATGA T^CATGA
SacII 3578 CCGCGG CCGC^GG
SexAI 956 ACCAGGT A^CC[AT]GGT
SfiI 873 GGCCATCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 285 CCCGGG CCC^GGG
Tth111I 1284 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 285 CCCGGG C^CCGGG
XmaI 285 CCCGGG C^CCGGG
XmnI 1719 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 237 CGCG CG^CG
3579 CGCG
AcsI 1723 GAATTC [GA]^AATT[CT]
1932 AAATTC
Aor51HI 143 AGCGCT AGC^GCT
512 AGCGCT
ApaI 282 GGGCCC GGGCC^C
1031 GGGCCC
ApoI 1723 GAATTC [GA]^AATT[CT]
1932 AAATTC
AseI 2332 ATTAAT AT^TAAT
2383 ATTAAT
AsnI 2332 ATTAAT AT^TAAT
2383 ATTAAT
BamHI 1861 GGATCC G^GATCC
3386 GGATCC
BbvCI 2465 CCTCAGC CCTCAGC(-5/-2)
2826 GCTGAGG
BfaI 407 CTAG C^TAG
2890 CTAG
BsaAI 717 CACGTA [CT]AC^GT[GA]
817 TACGTG
BsiEI 68 CGGCCG CG[GA][CT]^CG
94 CGGCCG
BsmI 3016 GAATGC GAATGC(1/-1)
3237 GCATTC
BsrDI 3042 CATTGC GCAATG(2/0)
3153 GCAATG
BstEII 741 GGTCACC G^GT[ACGT]ACC
2532 GGTGACC
BstPI 741 GGTCACC G^GT[ACGT]ACC
2532 GGTGACC
BstUI 237 CGCG CG^CG
3579 CGCG
EagI 68 CGGCCG C^GGCCG
94 CGGCCG
EclXI 68 CGGCCG C^GGCCG
94 CGGCCG
Eco47III 143 AGCGCT AGC^GCT
512 AGCGCT
Eco52I 68 CGGCCG C^GGCCG
94 CGGCCG
EcoO65I 741 GGTCACC G^GT[ACGT]ACC
2532 GGTGACC
EcoRV 1798 GATATC GAT^ATC
2482 GATATC
HindIII 1043 AAGCTT A^AGCTT
1967 AAGCTT
MaeI 407 CTAG C^TAG
2890 CTAG
MvnI 237 CGCG CG^CG
3579 CGCG
NaeI 70 GCCGGC GCC^GGC
883 GCCGGC
NgoMIV 70 GCCGGC G^CCGGC
883 GCCGGC
NspI 1974 GCATGT [GA]CATG^[CT]
2846 ACATGC
PshBI 2332 ATTAAT AT^TAAT
2383 ATTAAT
SapI 822 GCTCTTC GCTCTTC(1/4)
831 GCTCTTC
ScaI 2501 AGTACT AGT^ACT
3566 AGTACT
SmlI 600 CTTGAG C^T[CT][GA]AG
631 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccIII AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BclI BcnI BglI BmyI BpmI
BpuAI BsaBI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EarI Eco81I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII