Restriction map for
pFN21AB7287
(Linear) (Six-base) MAPSORT of: KIBB7287.seq Check: 4124 from: 1 to: 3753
KIBB7287.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 316 1199 3753
Size: 316 883 2554
Fragments arranged by size:
2554 883 316
AccI GT'mk_AC
Cuts at: 0 2627 3753
Size: 2627 1126
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1814 1877 2590 3439 3746 3753
Size: 1814 63 713 849 307 7
Fragments arranged by size:
1814 849 713 307 63 7
AfeI AGC'GCT
Cuts at: 0 145 514 3753
Size: 145 369 3239
Fragments arranged by size:
3239 369 145
AflIII A'CryG_T
Cuts at: 0 716 3753
Size: 716 3037
AleI CACnn'nnGTG
Cuts at: 0 748 1442 2518 3753
Size: 748 694 1076 1235
Fragments arranged by size:
1235 1076 748 694
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 916 3753
Size: 916 2837
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2116 2148 3753
Size: 2116 32 1605
Fragments arranged by size:
2116 1605 32
AlwNI CAG_nnn'CTG
Cuts at: 0 105 308 771 1302 2754 3712 3753
Size: 105 203 463 531 1452 958 41
Fragments arranged by size:
1452 958 531 463 203 105 41
ApaI G_GGCC'C
Cuts at: 0 286 1035 3753
Size: 286 749 2718
Fragments arranged by size:
2718 749 286
ApoI r'AATT_y
Cuts at: 0 1723 1932 3753
Size: 1723 209 1821
Fragments arranged by size:
1821 1723 209
AseI AT'TA_AT
Cuts at: 0 2333 2384 3753
Size: 2333 51 1369
Fragments arranged by size:
2333 1369 51
AvaI C'yCGr_G
Cuts at: 0 192 285 488 3753
Size: 192 93 203 3265
Fragments arranged by size:
3265 203 192 93
BamHI G'GATC_C
Cuts at: 0 1861 3386 3753
Size: 1861 1525 367
Fragments arranged by size:
1861 1525 367
BanI G'GyrC_C
Cuts at: 0 158 313 892 2395 3730 3753
Size: 158 155 579 1503 1335 23
Fragments arranged by size:
1503 1335 579 158 155 23
BanII G_rGCy'C
Cuts at: 0 8 286 797 1035 1325 1421 1822 1873
Size: 8 278 511 238 290 96 401 51
Cuts at: 1873 3720 3753
Size: 1847 33
Fragments arranged by size:
1847 511 401 290 278 238 96 51
33 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 315 2502 3077 3167 3558 3753
Size: 315 2187 575 90 391 195
Fragments arranged by size:
2187 575 391 315 195 90
BbvCI CC'TCA_GC
Cuts at: 0 2466 2827 3753
Size: 2466 361 926
Fragments arranged by size:
2466 926 361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1309 1343 3753
Size: 1309 34 2410
Fragments arranged by size:
2410 1309 34
BclI T'GATC_A
Cuts at: 0 1157 1466 2588 3753
Size: 1157 309 1122 1165
Fragments arranged by size:
1165 1157 1122 309
BglI GCCn_nnn'nGGC
Cuts at: 0 29 102 880 3753
Size: 29 73 778 2873
Fragments arranged by size:
2873 778 73 29
BglII A'GATC_T
Cuts at: 0 3455 3753
Size: 3455 298
Bme1580I G_kGCm'C
Cuts at: 0 161 286 405 895 1035 3753
Size: 161 125 119 490 140 2718
Fragments arranged by size:
2718 490 161 140 125 119
BmgBI CAC'GTC
Cuts at: 0 567 3753
Size: 567 3186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1072 1108 1646 1887 2076 2119 2776 3734
Size: 1072 36 538 241 189 43 657 958
Cuts at: 3734 3753
Size: 19
Fragments arranged by size:
1072 958 657 538 241 189 43 36
19
Bpu10I CC'TnA_GC
Cuts at: 0 1295 1415 2466 2827 3753
Size: 1295 120 1051 361 926
Fragments arranged by size:
1295 1051 926 361 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 621 3753
Size: 616 5 3132
Fragments arranged by size:
3132 616 5
BsaI GGTCTCn'nnnn_
Cuts at: 0 1462 2332 2614 3397 3753
Size: 1462 870 282 783 356
Fragments arranged by size:
1462 870 783 356 282
BsaAI yAC'GTr
Cuts at: 0 719 819 3753
Size: 719 100 2934
Fragments arranged by size:
2934 719 100
BsaBI GATnn'nnATC
Cuts at: 0 1216 1525 2599 3268 3753
Size: 1216 309 1074 669 485
Fragments arranged by size:
1216 1074 669 485 309
BsaHI Gr'CG_yC
Cuts at: 0 61 3753
Size: 61 3692
BsaWI w'CCGG_w
Cuts at: 0 1829 2312 2852 3260 3753
Size: 1829 483 540 408 493
Fragments arranged by size:
1829 540 493 483 408
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 333 363 694 724 1824 1854 2092 2122
Size: 333 30 331 30 1100 30 238 30
Cuts at: 2122 3753
Size: 1631
Fragments arranged by size:
1631 1100 333 331 238 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1812 2818 2926 3415 3753
Size: 1812 1006 108 489 338
Fragments arranged by size:
1812 1006 489 338 108
BseYI C'CCAG_C
Cuts at: 0 279 645 1034 2866 3697 3753
Size: 279 366 389 1832 831 56
Fragments arranged by size:
1832 831 389 366 279 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 2236 2561 3753
Size: 373 1863 325 1192
Fragments arranged by size:
1863 1192 373 325
BsiEI CG_ry'CG
Cuts at: 0 71 97 3753
Size: 71 26 3656
Fragments arranged by size:
3656 71 26
BsiHKAI G_wGCw'C
Cuts at: 0 8 824 1133 1190 1325 1822 2105 3186
Size: 8 816 309 57 135 497 283 1081
Cuts at: 3186 3753
Size: 567
Fragments arranged by size:
1081 816 567 497 309 283 135 57
8
BsmI GAATG_Cn'
Cuts at: 0 3022 3237 3753
Size: 3022 215 516
Fragments arranged by size:
3022 516 215
Bsp1286I G_dGCh'C
Cuts at: 0 8 161 286 405 797 824 895 1035
Size: 8 153 125 119 392 27 71 140
Cuts at: 1035 1133 1190 1325 1421 1822 1873 2105 3186
Size: 98 57 135 96 401 51 232 1081
Cuts at: 3186 3720 3753
Size: 534 33
Fragments arranged by size:
1081 534 401 392 232 153 140 135
125 119 98 96 71 57 51 33
27 8
BspEI T'CCGG_A
Cuts at: 0 2312 2852 3260 3753
Size: 2312 540 408 493
Fragments arranged by size:
2312 540 493 408
BspHI T'CATG_A
Cuts at: 0 2591 3753
Size: 2591 1162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 316 731 897 1199 3753
Size: 316 415 166 302 2554
Fragments arranged by size:
2554 415 316 302 166
BsrBI CCG'CTC
Cuts at: 0 344 630 864 1113 3753
Size: 344 286 234 249 2640
Fragments arranged by size:
2640 344 286 249 234
BsrDI GCAATG_nn'
Cuts at: 0 3041 3160 3753
Size: 3041 119 593
Fragments arranged by size:
3041 593 119
BsrFI r'CCGG_y
Cuts at: 0 70 658 883 3677 3753
Size: 70 588 225 2794 76
Fragments arranged by size:
2794 588 225 76 70
BssSI C'ACGA_G
Cuts at: 0 1117 3753
Size: 1117 2636
BstAPI GCAn_nnn'nTGC
Cuts at: 0 105 3753
Size: 105 3648
BstEII G'GTnAC_C
Cuts at: 0 741 2532 3753
Size: 741 1791 1221
Fragments arranged by size:
1791 1221 741
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2874 3753
Size: 2874 879
BstYI r'GATC_y
Cuts at: 0 767 1211 1861 3386 3455 3753
Size: 767 444 650 1525 69 298
Fragments arranged by size:
1525 767 650 444 298 69
Bsu36I CC'TnA_GG
Cuts at: 0 1729 1769 1991 3753
Size: 1729 40 222 1762
Fragments arranged by size:
1762 1729 222 40
BtgI C'CryG_G
Cuts at: 0 620 1704 2528 2742 3276 3578 3753
Size: 620 1084 824 214 534 302 175
Fragments arranged by size:
1084 824 620 534 302 214 175
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1207 3753
Size: 1207 2546
BtsI GCAGTG_nn'
Cuts at: 0 354 980 1359 1437 2585 2701 3753
Size: 354 626 379 78 1148 116 1052
Fragments arranged by size:
1148 1052 626 379 354 116 78
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2649 2684 3753
Size: 2649 35 1069
Fragments arranged by size:
2649 1069 35
DraI TTT'AAA
Cuts at: 0 2085 3753
Size: 2085 1668
DrdI GACnn_nn'nnGTC
Cuts at: 0 3690 3753
Size: 3690 63
EaeI y'GGCC_r
Cuts at: 0 68 94 752 881 2525 2714 3122 3381
Size: 68 26 658 129 1644 189 408 259
Cuts at: 3381 3575 3753
Size: 194 178
Fragments arranged by size:
1644 658 408 259 194 189 178 129
68 26
EagI C'GGCC_G
Cuts at: 0 68 94 3753
Size: 68 26 3659
Fragments arranged by size:
3659 68 26
EarI CTCTTCn'nnn_
Cuts at: 0 829 838 1691 2046 3510 3753
Size: 829 9 853 355 1464 243
Fragments arranged by size:
1464 853 829 355 243 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3696 3753
Size: 3696 57
EcoICRI GAG'CTC
Cuts at: 0 6 1323 1820 3753
Size: 6 1317 497 1933
Fragments arranged by size:
1933 1317 497 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1072 1108 1646 1814 1877 1887 2076 2119
Size: 1072 36 538 168 63 10 189 43
Cuts at: 2119 2590 2776 3439 3734 3746 3753
Size: 471 186 663 295 12 7
Fragments arranged by size:
1072 663 538 471 295 189 186 168
63 43 36 12 10 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2893 3753
Size: 2893 860
EcoRI G'AATT_C
Cuts at: 0 1723 3753
Size: 1723 2030
EcoRV GAT'ATC
Cuts at: 0 1800 2484 3753
Size: 1800 684 1269
Fragments arranged by size:
1800 1269 684
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2160 3753
Size: 2160 1593
FseI GG_CCGG'CC
Cuts at: 0 887 3753
Size: 887 2866
FspI TGC'GCA
Cuts at: 0 472 3753
Size: 472 3281
HaeII r_GCGC'y
Cuts at: 0 89 147 265 516 1041 3308 3753
Size: 89 58 118 251 525 2267 445
Fragments arranged by size:
2267 525 445 251 118 89 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 694 726 1573 1605 2045 2077 2473 2505
Size: 694 32 847 32 440 32 396 32
Cuts at: 2505 2968 3000 3440 3472 3753
Size: 463 32 440 32 281
Fragments arranged by size:
847 694 463 440 440 396 281 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1512 3753
Size: 1512 2241
HindIII A'AGCT_T
Cuts at: 0 1043 1967 3753
Size: 1043 924 1786
Fragments arranged by size:
1786 1043 924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2308 3546 3753
Size: 2308 1238 207
Fragments arranged by size:
2308 1238 207
MscI TGG'CCA
Cuts at: 0 754 2527 2716 3753
Size: 754 1773 189 1037
Fragments arranged by size:
1773 1037 754 189
MslI CAynn'nnrTG
Cuts at: 0 748 988 1226 1442 2518 3118 3124 3179
Size: 748 240 238 216 1076 600 6 55
Cuts at: 3179 3753
Size: 574
Fragments arranged by size:
1076 748 600 574 240 238 216 55
6
MspA1I CmG'CkG
Cuts at: 0 102 173 179 246 338 356 362 734
Size: 102 71 6 67 92 18 6 372
Cuts at: 734 858 1000 2470 2754 3580 3701 3753
Size: 124 142 1470 284 826 121 52
Fragments arranged by size:
1470 826 372 284 142 124 121 102
92 71 67 52 18 6 6
NaeI GCC'GGC
Cuts at: 0 72 885 3753
Size: 72 813 2868
Fragments arranged by size:
2868 813 72
NcoI C'CATG_G
Cuts at: 0 620 1704 2528 2742 3753
Size: 620 1084 824 214 1011
Fragments arranged by size:
1084 1011 824 620 214
NgoMIV G'CCGG_C
Cuts at: 0 70 883 3753
Size: 70 813 2870
Fragments arranged by size:
2870 813 70
NspI r_CATG'y
Cuts at: 0 1978 2850 3753
Size: 1978 872 903
Fragments arranged by size:
1978 903 872
PacI TTA_AT'TAA
Cuts at: 0 1760 3753
Size: 1760 1993
PasI CC'CwG_GG
Cuts at: 0 1636 3753
Size: 1636 2117
PflMI CCAn_nnn'nTGG
Cuts at: 0 771 963 3012 3753
Size: 771 192 2049 741
Fragments arranged by size:
2049 771 741 192
PfoI T'CCnGG_A
Cuts at: 0 326 1049 1148 1864 3753
Size: 326 723 99 716 1889
Fragments arranged by size:
1889 723 716 326 99
PsiI TTA'TAA
Cuts at: 0 2291 3753
Size: 2291 1462
PspOMI G'GGCC_C
Cuts at: 0 282 1031 3753
Size: 282 749 2722
Fragments arranged by size:
2722 749 282
PstI C_TGCA'G
Cuts at: 0 728 908 1196 3753
Size: 728 180 288 2557
Fragments arranged by size:
2557 728 288 180
PvuII CAG'CTG
Cuts at: 0 246 1000 2470 2754 3753
Size: 246 754 1470 284 999
Fragments arranged by size:
1470 999 754 284 246
SacI G_AGCT'C
Cuts at: 0 8 1325 1822 3753
Size: 8 1317 497 1931
Fragments arranged by size:
1931 1317 497 8
SacII CC_GC'GG
Cuts at: 0 3581 3753
Size: 3581 172
SapI GCTCTTCn'nnn_
Cuts at: 0 829 838 3753
Size: 829 9 2915
Fragments arranged by size:
2915 829 9
ScaI AGT'ACT
Cuts at: 0 2503 3568 3753
Size: 2503 1065 185
Fragments arranged by size:
2503 1065 185
SexAI A'CCwGG_T
Cuts at: 0 956 3753
Size: 956 2797
SfcI C'TryA_G
Cuts at: 0 438 724 843 904 1192 3753
Size: 438 286 119 61 288 2561
Fragments arranged by size:
2561 438 288 286 119 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 880 3753
Size: 880 2873
SmaI CCC'GGG
Cuts at: 0 287 3753
Size: 287 3466
SmlI C'TyrA_G
Cuts at: 0 600 631 3753
Size: 600 31 3122
Fragments arranged by size:
3122 600 31
StuI AGG'CCT
Cuts at: 0 782 2636 3134 3753
Size: 782 1854 498 619
Fragments arranged by size:
1854 782 619 498
StyI C'CwwG_G
Cuts at: 0 620 1704 2528 2690 2742 3753
Size: 620 1084 824 162 52 1011
Fragments arranged by size:
1084 1011 824 620 162 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 738 778 2383 2915 3089 3711 3753
Size: 738 40 1605 532 174 622 42
Fragments arranged by size:
1605 738 622 532 174 42 40
TatI w'GTAC_w
Cuts at: 0 728 2501 2759 2790 3566 3753
Size: 728 1773 258 31 776 187
Fragments arranged by size:
1773 776 728 258 187 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 333 365 1824 1856 3753
Size: 333 32 1459 32 1897
Fragments arranged by size:
1897 1459 333 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1287 3753
Size: 1287 2466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 768 1083 1662 2724 3753
Size: 768 315 579 1062 1029
Fragments arranged by size:
1062 1029 768 579 315
XmaI C'CCGG_G
Cuts at: 0 285 3753
Size: 285 3468
XmnI GAAnn'nnTTC
Cuts at: 0 1723 3753
Size: 1723 2030
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaI ApoI AseI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BclI BglI BglII Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII HindIII MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PacI PasI PflMI PfoI PsiI PspOMI
PstI PvuII SacI SacII SapI ScaI SexAI SfcI
SfiI SmaI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI ApaLI AscI
AsiSI AvrII BaeI BbeI BciVI BfrBI BlpI BmrI
BmtI BplI BsiWI BsmBI BsrGI BssHII BstBI BstZ17I
ClaI DraIII EcoO109I FspAI HpaI KasI KpnI MfeI
MluI NarI NdeI NheI NotI NruI NsiI PciI
PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PvuI
RsrII SalI SanDI SbfI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI ZraI