Restriction map for
pFN21AB7282
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BcgI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaHI
BseAI BsiEI BsiWI BspDI BspEI
BspMI BsrFI BssHII BssSI BstEII
BstPI CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FbaI FseI FspI HgaI Hin1I
HindIII KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI PacI
PaeR7I PflFI PinAI PmeI PshBI
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 2013 GTATAC GT^[AC][GT]AC
AclI 83 AACGTT AA^CGTT
AlwNI 1409 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 580 CCTGAGG CC^T[ACGT]AGG
BalI 1933 TGGCCA TGG^CCA
BanI 1941 GGTGCC G^G[CT][GA]CC
BfaI 2003 CTAG C^TAG
BglII 555 AGATCT A^GATCT
BsaBI 556 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1565 GGTCTC GGTCTC(1/5)
BsaWI 1028 ACCGGA [AT]^CCGG[AT]
BsmI 1980 GCATTC GAATGC(1/-1)
Bsp1407I 611 TGTACA T^GTACA
BspHI 1785 TCATGA T^CATGA
BspLU11I 2009 ACATGT A^CATGT
BsrBI 1333 GAGCGG CCGCTC(-3/-3)
BsrGI 611 TGTACA T^GTACA
Bst1107I 2013 GTATAC GTA^TAC
BstAPI 1456 GCAAAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 555 AGATCT [GA]^GATC[CT]
BstZ17I 2013 GTATAC GTA^TAC
Bsu36I 580 CCTGAGG CC^T[ACGT]AGG
EaeI 1933 TGGCCA [CT]^GGCC[GA]
Eco81I 580 CCTGAGG CC^T[ACGT]AGG
EcoRI 2037 GAATTC G^AATTC
HaeII 1246 AGCGCC [GA]GCGC^[CT]
HpaI 339 GTTAAC GTT^AAC
MaeI 2003 CTAG C^TAG
MamI 556 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 555 AGATCT [GA]^GATC[CT]
MluNI 1933 TGGCCA TGG^CCA
MscI 1933 TGGCCA TGG^CCA
NruI 1017 TCGCGA TCG^CGA
PshAI 1157 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 83 AACGTT AA^CGTT
RcaI 1785 TCATGA T^CATGA
ScaI 1894 AGTACT AGT^ACT
SspBI 611 TGTACA T^GTACA
SspI 416 AATATT AAT^ATT
StuI 2093 AGGCCT AGG^CCT
XcmI 1862 CCACTTCCCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 555 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 34 enzymes) #
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AccII 1018 CGCG CG^CG
2030 CGCG
AlwI 918 GATCC GGATC(4/5)
1224 GATCC
AvaI 355 CTCGGG C^[CT]CG[GA]G
362 CCCGAG
BbrPI 1560 CACGTG CAC^GTG
1956 CACGTG
BbvCI 1530 CCTCAGC CCTCAGC(-5/-2)
1946 CCTCAGC
BsmBI 463 GAGACG CGTCTC(1/5)
870 GAGACG
BsoBI 355 CTCGGG C^[CT]CG[GA]G
362 CCCGAG
BstBI 758 TTCGAA TT^CGAA
838 TTCGAA
BstUI 1018 CGCG CG^CG
2030 CGCG
CfoI 1247 GCGC GCG^C
2031 GCGC
EarI 1491 CTCTTC CTCTTC(1/4)
1393 GAAGAG
EcoNI 576 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1628 CCTGTATGAGG
EcoT14I 1004 CCATGG C^C[AT][AT]GG
1118 CCATGG
EcoT22I 1 ATGCAT ATGCA^T
250 ATGCAT
FauI 1928 CCCGC CCCGC(4/6)
1335 GCGGG
HhaI 1247 GCGC GCG^C
2031 GCGC
HinP1I 1247 GCGC G^CGC
2031 GCGC
HincII 339 GTTAAC GT[CT]^[GA]AC
1123 GTTGAC
HindII 339 GTTAAC GT[CT]^[GA]AC
1123 GTTGAC
Ksp632I 1491 CTCTTC CTCTTC(1/4)
1393 GAAGAG
MvnI 1018 CGCG CG^CG
2030 CGCG
NcoI 1004 CCATGG C^CATGG
1118 CCATGG
NsiI 1 ATGCAT ATGCA^T
250 ATGCAT
NspI 2009 ACATGT [GA]CATG^[CT]
2054 ACATGC
NspV 758 TTCGAA TT^CGAA
838 TTCGAA
PflMI 942 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1065 CCATCTCTTGG
PmaCI 1560 CACGTG CAC^GTG
1956 CACGTG
PmlI 1560 CACGTG CAC^GTG
1956 CACGTG
PstI 724 CTGCAG CTGCA^G
1379 CTGCAG
PvuII 1604 CAGCTG CAG^CTG
1621 CAGCTG
SacI 1345 GAGCTC GAGCT^C
1833 GAGCTC
SfuI 758 TTCGAA TT^CGAA
838 TTCGAA
StyI 1004 CCATGG C^C[AT][AT]GG
1118 CCATGG
Van91I 942 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1065 CCATCTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApoI AspHI AvaII BanII BbsI
BbvI BciVI BcnI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I