(Circular) (Six-base) MAPSORT of: pFN21AB7282.seq Check: 5031 from: 1 to: 6820 pFN21AB7282 6820 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4630 278 Size: 53 83 186 936 3094 2468 Fragments arranged by size: 3094 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4019 4163 1937 Size: 2082 144 4594 Fragments arranged by size: 4594 2082 144 Acc65I G'GTAC_C Cuts at: 4141 4141 Size: 6820 AclI AA'CG_TT Cuts at: 1563 2089 4947 5320 1563 Size: 526 2858 373 3063 Fragments arranged by size: 3063 2858 526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4883 6267 6535 1632 Size: 3251 1384 268 1917 Fragments arranged by size: 3251 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6623 AflIII A'CryG_T Cuts at: 1203 3564 3960 4014 5719 6696 1203 Size: 2361 396 54 1705 977 1327 Fragments arranged by size: 2361 1705 1327 977 396 54 AgeI A'CCGG_T Cuts at: 5651 5651 Size: 6820 AhdI GACnn_n'nnGTC Cuts at: 5549 5549 Size: 6820 AleI CACnn'nnGTG Cuts at: 1166 2657 3224 3476 3650 1166 Size: 1491 567 252 174 4336 Fragments arranged by size: 4336 1491 567 252 174 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6820 AlwNI CAG_nnn'CTG Cuts at: 3419 4563 6135 3419 Size: 1144 1572 4104 Fragments arranged by size: 4104 1572 1144 ApaLI G'TGCA_C Cuts at: 4877 6033 4877 Size: 1156 5664 ApoI r'AATT_y Cuts at: 1493 2543 2868 4042 4129 4355 4507 6616 1493 Size: 1050 325 1174 87 226 152 2109 1697 Fragments arranged by size: 2109 1697 1174 1050 325 226 152 87 AseI AT'TA_AT Cuts at: 160 1032 5374 160 Size: 872 4342 1606 Fragments arranged by size: 4342 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6820 AvaI C'yCGr_G Cuts at: 1870 1943 2360 2367 4145 1870 Size: 73 417 7 1778 4545 Fragments arranged by size: 4545 1778 417 73 7 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3111 3144 3111 Size: 33 6787 BamHI G'GATC_C Cuts at: 4150 4517 4150 Size: 367 6453 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3946 4141 5596 618 Size: 325 217 627 2159 195 1455 1842 Fragments arranged by size: 2159 1842 1455 627 325 217 195 BanII G_rGCy'C Cuts at: 1391 2648 3354 3842 4139 1391 Size: 1257 706 488 297 4072 Fragments arranged by size: 4072 1257 706 488 297 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2362 2964 3021 928 Size: 971 463 602 57 4727 Fragments arranged by size: 4727 971 602 463 57 BbvCI CC'TCA_GC Cuts at: 3536 3952 3536 Size: 416 6404 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5011 5045 1903 Size: 34 50 34 2990 34 3678 Fragments arranged by size: 3678 2990 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3112 3672 3704 3786 4712 5922 3112 Size: 560 32 82 926 1210 4010 Fragments arranged by size: 4010 1210 926 560 82 32 BclI T'GATC_A Cuts at: 1548 1548 Size: 6820 BfrBI ATG'CAT Cuts at: 2008 2257 2008 Size: 249 6571 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5431 136 Size: 107 122 71 631 605 3759 1525 Fragments arranged by size: 3759 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2560 6815 2560 Size: 4255 2565 BlpI GC'TnA_GC Cuts at: 4237 4237 Size: 6820 Bme1580I G_kGCm'C Cuts at: 1790 2248 3951 4881 6037 1790 Size: 458 1703 930 1156 2573 Fragments arranged by size: 2573 1703 1156 930 458 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6820 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2651 3917 4551 Size: 416 53 83 399 24 1234 1266 634 Cuts at: 4551 5509 442 Size: 958 1753 Fragments arranged by size: 1753 1266 1234 958 634 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6820 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3663 3918 3967 3663 Size: 255 49 6516 Fragments arranged by size: 6516 255 49 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2219 2983 3441 5480 1239 Size: 803 177 764 458 2039 2579 Fragments arranged by size: 2579 2039 803 764 458 177 Bpu10I CC'TnA_GC Cuts at: 3536 3952 3536 Size: 416 6404 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4945 5810 6108 4945 Size: 865 298 5657 Fragments arranged by size: 5657 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3576 5483 882 Size: 617 2077 1907 2219 Fragments arranged by size: 2219 2077 1907 617 BsaAI yAC'GTr Cuts at: 493 1204 3304 3567 3963 4611 493 Size: 711 2100 263 396 648 2702 Fragments arranged by size: 2702 2100 711 648 396 263 BsaBI GATnn'nnATC Cuts at: 2565 6814 2565 Size: 4249 2571 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4627 5009 5644 275 Size: 53 83 186 936 3094 382 635 1451 Fragments arranged by size: 3094 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3033 5253 5651 5925 6072 6483 3033 Size: 2220 398 274 147 411 3370 Fragments arranged by size: 3370 2220 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3071 3101 1178 Size: 30 165 30 1668 30 4897 Fragments arranged by size: 4897 1668 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2216 2603 2638 3095 1186 Size: 1030 387 35 457 4911 Fragments arranged by size: 4911 1030 457 387 35 BseYI C'CCAG_C Cuts at: 3186 3257 3599 3607 6023 6519 3186 Size: 71 342 8 2416 496 3487 Fragments arranged by size: 3487 2416 496 342 71 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2952 3311 3828 1163 Size: 573 1216 359 517 4155 Fragments arranged by size: 4155 1216 573 517 359 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4270 5031 6059 1540 Size: 275 186 2269 761 1028 2301 Fragments arranged by size: 2301 2269 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3033 3309 3354 3842 4059 4881 4966 6037 3033 Size: 276 45 488 217 822 85 1071 3816 Fragments arranged by size: 3816 1071 822 488 276 217 85 45 BsmI GAATG_Cn' Cuts at: 3985 4331 4424 3985 Size: 346 93 6381 Fragments arranged by size: 6381 346 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2462 2869 2462 Size: 407 6413 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2248 2648 3033 3309 3354 3842 3951 Size: 399 458 400 385 276 45 488 109 Cuts at: 3951 4059 4139 4881 4966 6037 1391 Size: 108 80 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 488 458 400 399 385 276 109 108 85 80 45 BspEI T'CCGG_A Cuts at: 6483 6483 Size: 6820 BspHI T'CATG_A Cuts at: 3790 4709 6439 3790 Size: 919 1730 4171 Fragments arranged by size: 4171 1730 919 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4175 844 Size: 3331 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3340 4707 1394 Size: 429 1517 1367 3507 Fragments arranged by size: 3507 1517 1367 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2198 2397 3558 5315 5489 65 Size: 1183 950 199 1161 1757 174 1396 Fragments arranged by size: 1757 1396 1183 1161 950 199 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5464 5651 1700 Size: 110 3654 187 2869 Fragments arranged by size: 3654 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2616 96 Size: 2520 4300 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6820 BssSI C'ACGA_G Cuts at: 4880 5892 4880 Size: 1012 5808 BstAPI GCAn_nnn'nTGC Cuts at: 794 3467 794 Size: 2673 4147 BstBI TT'CG_AA Cuts at: 2764 2844 5624 2764 Size: 80 2780 3960 Fragments arranged by size: 3960 2780 80 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6820 BstXI CCAn_nnnn'nTGG Cuts at: 2393 2782 3478 2393 Size: 389 696 5735 Fragments arranged by size: 5735 696 389 BstYI r'GATC_y Cuts at: 1972 2560 4150 4517 4902 4919 6371 6457 6469 Size: 588 1590 367 385 17 1452 86 12 Cuts at: 6469 6815 1972 Size: 346 1977 Fragments arranged by size: 1977 1590 1452 588 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 4020 4020 Size: 6820 Bsu36I CC'TnA_GG Cuts at: 2586 2586 Size: 6820 BtgI C'CryG_G Cuts at: 513 1065 2652 3009 3123 513 Size: 552 1587 357 114 4210 Fragments arranged by size: 4210 1587 552 357 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3039 487 Size: 683 56 1813 4268 Fragments arranged by size: 4268 1813 683 56 BtsI GCAGTG_nn' Cuts at: 2248 2409 2531 3381 4336 5130 5150 2248 Size: 161 122 850 955 794 20 3918 Fragments arranged by size: 3918 955 850 794 161 122 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6820 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6785 DraI TTT'AAA Cuts at: 4124 4124 Size: 6820 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5827 809 Size: 507 4511 1802 Fragments arranged by size: 4511 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3938 4267 5156 Size: 54 1425 50 275 18 2108 329 889 Cuts at: 5156 6785 8 Size: 1629 43 Fragments arranged by size: 2108 1629 1425 889 329 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4267 1537 Size: 275 2455 4090 Fragments arranged by size: 4090 2455 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3393 3502 4750 1353 Size: 2040 109 1248 3423 Fragments arranged by size: 3423 2040 1248 109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5407 5781 5927 387 Size: 1064 138 3818 374 146 1280 Fragments arranged by size: 3818 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3352 3840 3352 Size: 488 6332 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2219 2983 3441 4883 5480 6267 Size: 393 410 177 764 458 1442 597 787 Cuts at: 6267 6535 1239 Size: 268 1524 Fragments arranged by size: 1524 1442 787 764 597 458 410 393 268 177 EcoNI CCTnn'n_nnAGG Cuts at: 2585 3637 2585 Size: 1052 5768 EcoO109I rG'GnC_Cy Cuts at: 2364 3168 3603 2364 Size: 804 435 5581 Fragments arranged by size: 5581 804 435 EcoRI G'AATT_C Cuts at: 4042 4129 4507 4042 Size: 87 378 6355 Fragments arranged by size: 6355 378 87 FspI TGC'GCA Cuts at: 5326 5326 Size: 6820 HaeII r_GCGC'y Cuts at: 1678 3255 5967 1678 Size: 1577 2712 2531 Fragments arranged by size: 2712 2531 1577 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3071 3103 3387 3419 3760 Size: 32 135 32 1499 32 284 32 341 Cuts at: 3760 3792 5461 5493 5535 5567 1373 Size: 32 1669 32 42 32 2626 Fragments arranged by size: 2626 1669 1499 341 284 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2346 3130 4164 4410 669 Size: 988 281 408 784 1034 246 3079 Fragments arranged by size: 3079 1034 988 784 408 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6820 HpaI GTT'AAC Cuts at: 2346 4410 2346 Size: 2064 4756 KpnI G_GTAC'C Cuts at: 4145 4145 Size: 6820 MfeI C'AATT_G Cuts at: 4419 4419 Size: 6820 MluI A'CGCG_T Cuts at: 6696 6696 Size: 6820 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5934 6118 1305 Size: 4629 184 2007 Fragments arranged by size: 4629 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3940 10 Size: 54 1425 343 2108 2890 Fragments arranged by size: 2890 2108 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2391 2402 2657 3224 3476 3650 4778 Size: 648 1225 11 255 567 252 174 1128 Cuts at: 4778 5137 5296 518 Size: 359 159 2042 Fragments arranged by size: 2042 1225 1128 648 567 359 255 252 174 159 11 MspA1I CmG'CkG Cuts at: 1606 3135 3611 3628 3936 4236 4913 6061 6306 Size: 1529 476 17 308 300 677 1148 245 Cuts at: 6306 1606 Size: 2120 Fragments arranged by size: 2120 1529 1148 677 476 308 300 245 17 NcoI C'CATG_G Cuts at: 513 1065 3009 3123 513 Size: 552 1944 114 4210 Fragments arranged by size: 4210 1944 552 114 NdeI CA'TA_TG Cuts at: 387 387 Size: 6820 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6820 NotI GC'GGCC_GC Cuts at: 4267 4267 Size: 6820 NruI TCG'CGA Cuts at: 3024 3024 Size: 6820 NsiI A_TGCA'T Cuts at: 2010 2259 2010 Size: 249 6571 NspI r_CATG'y Cuts at: 1132 1229 4018 4063 4178 5723 1132 Size: 97 2789 45 115 1545 2229 Fragments arranged by size: 2789 2229 1545 115 97 45 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6820 PciI A'CATG_T Cuts at: 4014 5719 4014 Size: 1705 5115 PflMI CCAn_nnn'nTGG Cuts at: 1108 2953 3076 1108 Size: 1845 123 4852 Fragments arranged by size: 4852 1845 123 PfoI T'CCnGG_A Cuts at: 3231 3872 3231 Size: 641 6179 PmeI GTTT'AAAC Cuts at: 4124 4124 Size: 6820 PmlI CAC'GTG Cuts at: 3567 3963 3567 Size: 396 6424 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4889 4921 6402 6434 4889 Size: 32 1481 32 5275 Fragments arranged by size: 5275 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2364 3168 3603 2364 Size: 804 435 5581 Fragments arranged by size: 5581 804 435 PshAI GACnn'nnGTC Cuts at: 1613 3166 1613 Size: 1553 5267 PsiI TTA'TAA Cuts at: 4390 4390 Size: 6820 PstI C_TGCA'G Cuts at: 830 2733 3388 4172 830 Size: 1903 655 784 3478 Fragments arranged by size: 3478 1903 784 655 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6820 PvuII CAG'CTG Cuts at: 3611 3628 3611 Size: 17 6803 SacI G_AGCT'C Cuts at: 3354 3842 3354 Size: 488 6332 SalI G'TCGA_C Cuts at: 1936 4162 1936 Size: 2226 4594 SanDI GG'GwC_CC Cuts at: 2364 3603 2364 Size: 1239 5581 SbfI CC_TGCA'GG Cuts at: 4172 4172 Size: 6820 ScaI AGT'ACT Cuts at: 3901 5068 6780 3901 Size: 1167 1712 3941 Fragments arranged by size: 3941 1712 1167 SfcI C'TryA_G Cuts at: 826 1045 2729 3384 3543 4104 4168 5303 5984 Size: 219 1684 655 159 561 64 1135 681 Cuts at: 5984 6175 826 Size: 191 1471 Fragments arranged by size: 1684 1471 1135 681 655 561 219 191 159 64 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6820 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6820 SmaI CCC'GGG Cuts at: 1872 4147 1872 Size: 2275 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4924 5825 6087 820 Size: 197 926 2981 901 262 1553 Fragments arranged by size: 2981 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4611 493 Size: 4118 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6820 SphI G_CATG'C Cuts at: 1132 4178 1132 Size: 3046 3774 SspI AAT'ATT Cuts at: 5 52 2423 4744 5 Size: 47 2371 2321 2081 Fragments arranged by size: 2371 2321 2081 47 StuI AGG'CCT Cuts at: 1517 4100 1517 Size: 2583 4237 StyI C'CwwG_G Cuts at: 513 1065 3009 3123 4259 513 Size: 552 1944 114 1136 3074 Fragments arranged by size: 3074 1944 1136 552 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2449 3062 4847 4864 5017 5202 5647 Size: 1255 869 613 1785 17 153 185 445 Cuts at: 5647 325 Size: 1498 Fragments arranged by size: 1785 1498 1255 869 613 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2616 3525 3899 Size: 274 80 33 51 1443 639 909 374 Cuts at: 3899 5066 6778 96 Size: 1167 1712 138 Fragments arranged by size: 1712 1443 1167 909 639 374 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6780 6812 6780 Size: 32 6788 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6820 XbaI T'CTAG_A Cuts at: 4156 4156 Size: 6820 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3874 3874 Size: 6820 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6820 XmaI C'CCGG_G Cuts at: 1870 4145 1870 Size: 2275 4545 XmnI GAAnn'nnTTC Cuts at: 4949 4949 Size: 6820 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4628 276 Size: 53 83 186 936 3094 2468 Fragments arranged by size: 3094 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV PacI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI