Restriction map for
pFN21AB7282
(Linear) (Six-base) MAPSORT of: KIBB7282.seq Check: 7551 from: 1 to: 2115
KIBB7282.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AccI GT'mk_AC
Cuts at: 0 2014 2115
Size: 2014 101
AclI AA'CG_TT
Cuts at: 0 84 2115
Size: 84 2031
AflIII A'CryG_T
Cuts at: 0 1559 1955 2009 2115
Size: 1559 396 54 106
Fragments arranged by size:
1559 396 106 54
AleI CACnn'nnGTG
Cuts at: 0 652 1219 1471 1645 2115
Size: 652 567 252 174 470
Fragments arranged by size:
652 567 470 252 174
AlwNI CAG_nnn'CTG
Cuts at: 0 1414 2115
Size: 1414 701
ApoI r'AATT_y
Cuts at: 0 538 863 2037 2115
Size: 538 325 1174 78
Fragments arranged by size:
1174 538 325 78
AvaI C'yCGr_G
Cuts at: 0 355 362 2115
Size: 355 7 1753
Fragments arranged by size:
1753 355 7
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1106 1139 2115
Size: 1106 33 976
Fragments arranged by size:
1106 976 33
BanI G'GyrC_C
Cuts at: 0 1941 2115
Size: 1941 174
BanII G_rGCy'C
Cuts at: 0 643 1349 1837 2115
Size: 643 706 488 278
Fragments arranged by size:
706 643 488 278
BbsI GAAGACnn'nnnn_
Cuts at: 0 357 959 1016 2115
Size: 357 602 57 1099
Fragments arranged by size:
1099 602 357 57
BbvCI CC'TCA_GC
Cuts at: 0 1531 1947 2115
Size: 1531 416 168
Fragments arranged by size:
1531 416 168
BciVI GTATCCnnnnn_n'
Cuts at: 0 1107 1667 1699 1781 2115
Size: 1107 560 32 82 334
Fragments arranged by size:
1107 560 334 82 32
BfrBI ATG'CAT
Cuts at: 0 3 252 2115
Size: 3 249 1863
Fragments arranged by size:
1863 249 3
BglII A'GATC_T
Cuts at: 0 555 2115
Size: 555 1560
Bme1580I G_kGCm'C
Cuts at: 0 243 1946 2115
Size: 243 1703 169
Fragments arranged by size:
1703 243 169
BmrI ACTGGGnnnn_n'
Cuts at: 0 646 1912 2115
Size: 646 1266 203
Fragments arranged by size:
1266 646 203
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1658 1913 1962 2115
Size: 1658 255 49 153
Fragments arranged by size:
1658 255 153 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 214 978 1436 2115
Size: 37 177 764 458 679
Fragments arranged by size:
764 679 458 177 37
Bpu10I CC'TnA_GC
Cuts at: 0 1531 1947 2115
Size: 1531 416 168
Fragments arranged by size:
1531 416 168
BsaI GGTCTCn'nnnn_
Cuts at: 0 1571 2115
Size: 1571 544
BsaAI yAC'GTr
Cuts at: 0 1299 1562 1958 2115
Size: 1299 263 396 157
Fragments arranged by size:
1299 396 263 157
BsaBI GATnn'nnATC
Cuts at: 0 560 2115
Size: 560 1555
BsaWI w'CCGG_w
Cuts at: 0 1028 2115
Size: 1028 1087
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1066 1096 2115
Size: 1066 30 1019
Fragments arranged by size:
1066 1019 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 211 598 633 1090 2115
Size: 211 387 35 457 1025
Fragments arranged by size:
1025 457 387 211 35
BseYI C'CCAG_C
Cuts at: 0 1181 1252 1594 1602 2115
Size: 1181 71 342 8 513
Fragments arranged by size:
1181 513 342 71 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 947 1306 1823 2115
Size: 947 359 517 292
Fragments arranged by size:
947 517 359 292
BsiHKAI G_wGCw'C
Cuts at: 0 1028 1304 1349 1837 2054 2115
Size: 1028 276 45 488 217 61
Fragments arranged by size:
1028 488 276 217 61 45
BsmI GAATG_Cn'
Cuts at: 0 1980 2115
Size: 1980 135
BsmBI CGTCTCn'nnnn_
Cuts at: 0 457 864 2115
Size: 457 407 1251
Fragments arranged by size:
1251 457 407
Bsp1286I G_dGCh'C
Cuts at: 0 243 643 1028 1304 1349 1837 1946 2054
Size: 243 400 385 276 45 488 109 108
Cuts at: 2054 2115
Size: 61
Fragments arranged by size:
488 400 385 276 243 109 108 61
45
BspHI T'CATG_A
Cuts at: 0 1785 2115
Size: 1785 330
BsrBI CCG'CTC
Cuts at: 0 1335 2115
Size: 1335 780
BsrDI GCAATG_nn'
Cuts at: 0 193 392 1553 2115
Size: 193 199 1161 562
Fragments arranged by size:
1161 562 199 193
BsrGI T'GTAC_A
Cuts at: 0 611 2115
Size: 611 1504
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1462 2115
Size: 1462 653
BstBI TT'CG_AA
Cuts at: 0 759 839 2115
Size: 759 80 1276
Fragments arranged by size:
1276 759 80
BstXI CCAn_nnnn'nTGG
Cuts at: 0 388 777 1473 2115
Size: 388 389 696 642
Fragments arranged by size:
696 642 389 388
BstYI r'GATC_y
Cuts at: 0 555 2115
Size: 555 1560
BstZ17I GTA'TAC
Cuts at: 0 2015 2115
Size: 2015 100
Bsu36I CC'TnA_GG
Cuts at: 0 581 2115
Size: 581 1534
BtgI C'CryG_G
Cuts at: 0 647 1004 1118 2115
Size: 647 357 114 997
Fragments arranged by size:
997 647 357 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1034 2115
Size: 1034 1081
BtsI GCAGTG_nn'
Cuts at: 0 243 404 526 1376 2115
Size: 243 161 122 850 739
Fragments arranged by size:
850 739 243 161 122
EaeI y'GGCC_r
Cuts at: 0 1933 2115
Size: 1933 182
EarI CTCTTCn'nnn_
Cuts at: 0 1388 1497 2115
Size: 1388 109 618
Fragments arranged by size:
1388 618 109
EcoICRI GAG'CTC
Cuts at: 0 1347 1835 2115
Size: 1347 488 280
Fragments arranged by size:
1347 488 280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 214 978 1436 2115
Size: 37 177 764 458 679
Fragments arranged by size:
764 679 458 177 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 580 1632 2115
Size: 580 1052 483
Fragments arranged by size:
1052 580 483
EcoO109I rG'GnC_Cy
Cuts at: 0 359 1163 1598 2115
Size: 359 804 435 517
Fragments arranged by size:
804 517 435 359
EcoRI G'AATT_C
Cuts at: 0 2037 2115
Size: 2037 78
HaeII r_GCGC'y
Cuts at: 0 1250 2115
Size: 1250 865
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1066 1098 1382 1414 1755 1787 2115
Size: 1066 32 284 32 341 32 328
Fragments arranged by size:
1066 341 328 284 32 32 32
HincII GTy'rAC
Cuts at: 0 341 1125 2115
Size: 341 784 990
Fragments arranged by size:
990 784 341
HpaI GTT'AAC
Cuts at: 0 341 2115
Size: 341 1774
MscI TGG'CCA
Cuts at: 0 1935 2115
Size: 1935 180
MslI CAynn'nnrTG
Cuts at: 0 386 397 652 1219 1471 1645 2115
Size: 386 11 255 567 252 174 470
Fragments arranged by size:
567 470 386 255 252 174 11
MspA1I CmG'CkG
Cuts at: 0 1130 1606 1623 1931 2115
Size: 1130 476 17 308 184
Fragments arranged by size:
1130 476 308 184 17
NcoI C'CATG_G
Cuts at: 0 1004 1118 2115
Size: 1004 114 997
Fragments arranged by size:
1004 997 114
NruI TCG'CGA
Cuts at: 0 1019 2115
Size: 1019 1096
NsiI A_TGCA'T
Cuts at: 0 5 254 2115
Size: 5 249 1861
Fragments arranged by size:
1861 249 5
NspI r_CATG'y
Cuts at: 0 2013 2058 2115
Size: 2013 45 57
Fragments arranged by size:
2013 57 45
PciI A'CATG_T
Cuts at: 0 2009 2115
Size: 2009 106
PflMI CCAn_nnn'nTGG
Cuts at: 0 948 1071 2115
Size: 948 123 1044
Fragments arranged by size:
1044 948 123
PfoI T'CCnGG_A
Cuts at: 0 1226 1867 2115
Size: 1226 641 248
Fragments arranged by size:
1226 641 248
PmlI CAC'GTG
Cuts at: 0 1562 1958 2115
Size: 1562 396 157
Fragments arranged by size:
1562 396 157
PpuMI rG'GwC_Cy
Cuts at: 0 359 1163 1598 2115
Size: 359 804 435 517
Fragments arranged by size:
804 517 435 359
PshAI GACnn'nnGTC
Cuts at: 0 1161 2115
Size: 1161 954
PstI C_TGCA'G
Cuts at: 0 728 1383 2115
Size: 728 655 732
Fragments arranged by size:
732 728 655
PvuII CAG'CTG
Cuts at: 0 1606 1623 2115
Size: 1606 17 492
Fragments arranged by size:
1606 492 17
SacI G_AGCT'C
Cuts at: 0 1349 1837 2115
Size: 1349 488 278
Fragments arranged by size:
1349 488 278
SanDI GG'GwC_CC
Cuts at: 0 359 1598 2115
Size: 359 1239 517
Fragments arranged by size:
1239 517 359
ScaI AGT'ACT
Cuts at: 0 1896 2115
Size: 1896 219
SfcI C'TryA_G
Cuts at: 0 724 1379 1538 2099 2115
Size: 724 655 159 561 16
Fragments arranged by size:
724 655 561 159 16
SspI AAT'ATT
Cuts at: 0 418 2115
Size: 418 1697
StuI AGG'CCT
Cuts at: 0 2095 2115
Size: 2095 20
StyI C'CwwG_G
Cuts at: 0 1004 1118 2115
Size: 1004 114 997
Fragments arranged by size:
1004 997 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 444 1057 2115
Size: 444 613 1058
Fragments arranged by size:
1058 613 444
TatI w'GTAC_w
Cuts at: 0 611 1520 1894 2115
Size: 611 909 374 221
Fragments arranged by size:
909 611 374 221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1869 2115
Size: 1869 246
Enzymes that do cut:
AccI AclI AflIII AleI AlwNI ApoI AvaI BaeI
BanI BanII BbsI BbvCI BciVI BfrBI BglII Bme1580I
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII HpaI
MscI MslI MspA1I NcoI NruI NsiI NspI PciI
PflMI PfoI PmlI PpuMI PshAI PstI PvuII SacI
SanDI ScaI SfcI SspI StuI StyI TaqII TatI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AcuI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BbeI BcgI BclI BglI BlpI BmgBI BmtI
BpuEI BsaHI BsiEI BsiWI BspEI BspMI BsrFI BssHII
BssSI BstEII ClaI CspCI DraI DraIII DrdI EagI
EciI EcoRV FalI FseI FspI FspAI HindIII KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI PacI PasI PmeI PpiI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI