Restriction map for
pFN21AB7262
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BbrPI BcgI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MseI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I Tth111I XbaI
XcmI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 42 CGCG CG^CG
AcyI 196 GGCGCC G[GA]^CG[CT]C
AflIII 89 ACATGT A^C[GA][CT]GT
AocI 589 CCTCAGG CC^T[ACGT]AGG
ApaI 314 GGGCCC GGGCC^C
Asp700I 1276 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 963 TGGCCA TGG^CCA
BamHI 594 GGATCC G^GATCC
BbeI 196 GGCGCC GGCGC^C
BbvCI 654 CCTCAGC CCTCAGC(-5/-2)
BciVI 1030 GTATCC GTATCC(6/5)
BfaI 1286 CTAG C^TAG
BpmI 1298 CTGGAG CTGGAG(16/14)
BsaHI 196 GGCGCC G[GA]^CG[CT]C
BseRI 24 CTCCTC GAGGAG(10/8)
BsmAI 582 GAGAC GTCTC(1/5)
BspLU11I 89 ACATGT A^CATGT
BsrDI 267 GCAATG GCAATG(2/0)
BssSI 1072 CACGAG CACGAG(-5/-1)
BstUI 42 CGCG CG^CG
BstXI 278 CCAAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 589 CCTCAGG CC^T[ACGT]AGG
Eco81I 589 CCTCAGG CC^T[ACGT]AGG
HgaI 1053 GCGTC GACGC(5/10)
Hin1I 196 GGCGCC G[GA]^CG[CT]C
KasI 196 GGCGCC G^GCGCC
MaeI 1286 CTAG C^TAG
MluNI 963 TGGCCA TGG^CCA
MscI 963 TGGCCA TGG^CCA
MslI 263 CACAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 42 CGCG CG^CG
NarI 196 GGCGCC GG^CGCC
NspI 89 ACATGT [GA]CATG^[CT]
PaeR7I 74 CTCGAG C^TCGAG
PflMI 1291 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 420 CTGCAG CTGCA^G
PvuII 423 CAGCTG CAG^CTG
SexAI 985 ACCAGGT A^CC[AT]GGT
SfcI 420 CTGCAG C^T[GA][CT]AG
SfoI 196 GGCGCC GGC^GCC
SmaI 630 CCCGGG CCC^GGG
SmlI 74 CTCGAG C^T[CT][GA]AG
TaqI 75 TCGA T^CGA
TfiI 1176 GAATC G^A[AT]TC
TthHB8I 75 TCGA T^CGA
Van91I 1291 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 74 CTCGAG C^TCGAG
XmaCI 630 CCCGGG C^CCGGG
XmaI 630 CCCGGG C^CCGGG
XmnI 1276 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AlwI 595 GATCC GGATC(4/5)
594 GGATC
AlwNI 956 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
1292 CAGACCCTG
BanII 77 GAGCCC G[GA]GC[CT]^C
314 GGGCCC
BbsI 768 GAAGAC GAAGAC(2/6)
1280 GTCTTC
BpuAI 768 GAAGAC GAAGAC(2/6)
1280 GTCTTC
BsgI 426 CTGCAC GTGCAG(16/14)
562 GTGCAG
BstAPI 830 GCATCCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
932 GCAAGATTTGC
EarI 111 GAAGAG CTCTTC(1/4)
550 GAAGAG
HaeII 196 GGCGCC [GA]GCGC^[CT]
658 AGCGCC
Ksp632I 111 GAAGAG CTCTTC(1/4)
550 GAAGAG
MaeII 772 ACGT A^CGT
1278 ACGT
MspA1I 423 CAGCTG C[AC]G^C[GT]G
493 CCGCTG
NcoI 105 CCATGG C^CATGG
573 CCATGG
PpuMI 17 GGGTCCT [GA]G^G[AT]CC[CT]
1010 GGGTCCT
SfaNI 610 GATGC GCATC(5/9)
830 GCATC
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BanI BbvI BcnI BglII BmyI
BsaJI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI StuI StyI TseI Tsp45I
TspRI XhoII