(Circular) (Six-base) MAPSORT of: pFN21AB7262.seq Check: 13 from: 1 to: 6058 pFN21AB7262 6058 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3868 278 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3401 1937 Size: 1464 4594 Acc65I G'GTAC_C Cuts at: 3379 3379 Size: 6058 AclI AA'CG_TT Cuts at: 1563 4185 4558 1563 Size: 2622 373 3063 Fragments arranged by size: 3063 2622 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2359 2677 2756 3330 4121 5505 5773 1632 Size: 727 318 79 574 791 1384 268 1917 Fragments arranged by size: 1917 1384 791 727 574 318 268 79 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5861 AflIII A'CryG_T Cuts at: 1203 2094 4957 5934 1203 Size: 891 2863 977 1327 Fragments arranged by size: 2863 1327 977 891 AgeI A'CCGG_T Cuts at: 4889 4889 Size: 6058 AhdI GACnn_n'nnGTC Cuts at: 4787 4787 Size: 6058 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6058 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6058 AlwNI CAG_nnn'CTG Cuts at: 2966 3302 3801 5373 2966 Size: 336 499 1572 3651 Fragments arranged by size: 3651 1572 499 336 ApaI G_GGCC'C Cuts at: 2323 2323 Size: 6058 ApaLI G'TGCA_C Cuts at: 4115 5271 4115 Size: 1156 4902 ApoI r'AATT_y Cuts at: 1493 3367 3593 3745 5854 1493 Size: 1874 226 152 2109 1697 Fragments arranged by size: 2109 1874 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4612 160 Size: 872 3580 1606 Fragments arranged by size: 3580 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 AvaI C'yCGr_G Cuts at: 1870 1943 2012 2079 2635 2912 3383 1870 Size: 73 69 67 556 277 471 4545 Fragments arranged by size: 4545 556 471 277 73 69 67 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3018 3051 3018 Size: 33 6025 BamHI G'GATC_C Cuts at: 2599 3388 3755 2599 Size: 789 367 4902 Fragments arranged by size: 4902 789 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2142 2201 2639 2799 3004 Size: 325 217 627 355 59 438 160 205 Cuts at: 3004 3379 4834 618 Size: 375 1455 1842 Fragments arranged by size: 1842 1455 627 438 375 355 325 217 205 160 59 BanII G_rGCy'C Cuts at: 1391 2086 2323 3377 1391 Size: 695 237 1054 4072 Fragments arranged by size: 4072 1054 695 237 BbeI G_GCGC'C Cuts at: 2205 2205 Size: 6058 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2780 3278 928 Size: 971 881 498 3708 Fragments arranged by size: 3708 971 881 498 BbvCI CC'TCA_GC Cuts at: 2660 2660 Size: 6058 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4249 4283 1903 Size: 34 2312 34 3678 Fragments arranged by size: 3678 2312 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3046 3950 5160 3046 Size: 904 1210 3944 Fragments arranged by size: 3944 1210 904 BclI T'GATC_A Cuts at: 1548 1548 Size: 6058 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4669 136 Size: 107 122 71 631 605 2997 1525 Fragments arranged by size: 2997 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2370 2445 3099 6053 2370 Size: 75 654 2954 2375 Fragments arranged by size: 2954 2375 654 75 BlpI GC'TnA_GC Cuts at: 3475 3475 Size: 6058 Bme1580I G_kGCm'C Cuts at: 1790 2323 2383 2642 4119 5275 1790 Size: 533 60 259 1477 1156 2573 Fragments arranged by size: 2573 1477 1156 533 259 60 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6058 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3789 4747 442 Size: 416 53 83 399 24 2372 958 1753 Fragments arranged by size: 2372 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6058 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2610 2610 Size: 6058 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3324 4718 1239 Size: 2085 1394 2579 Fragments arranged by size: 2579 2085 1394 Bpu10I CC'TnA_GC Cuts at: 2660 2660 Size: 6058 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4183 5048 5346 4183 Size: 865 298 4895 Fragments arranged by size: 4895 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4721 882 Size: 617 3222 2219 Fragments arranged by size: 3222 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3849 493 Size: 711 2645 2702 Fragments arranged by size: 2702 2645 711 BsaBI GATnn'nnATC Cuts at: 6052 6052 Size: 6058 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2202 3865 4247 4882 Size: 53 83 186 936 669 1663 382 635 Cuts at: 4882 275 Size: 1451 Fragments arranged by size: 1663 1451 936 669 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4491 4889 5163 5310 5721 4491 Size: 398 274 147 411 4828 Fragments arranged by size: 4828 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5833 Fragments arranged by size: 5833 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2020 1186 Size: 834 5224 BseYI C'CCAG_C Cuts at: 2038 2250 2485 2618 2644 2924 5261 5757 2038 Size: 212 235 133 26 280 2337 496 2339 Fragments arranged by size: 2339 2337 496 280 235 212 133 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2416 2588 1163 Size: 573 680 172 4633 Fragments arranged by size: 4633 680 573 172 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3508 4269 5297 1540 Size: 275 186 1507 761 1028 2301 Fragments arranged by size: 2301 1507 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4119 4204 5275 4119 Size: 85 1071 4902 Fragments arranged by size: 4902 1071 85 BsmI GAATG_Cn' Cuts at: 3569 3662 3569 Size: 93 5965 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 2323 2383 2642 3377 4119 4204 Size: 399 296 237 60 259 735 742 85 Cuts at: 4204 5275 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 735 399 296 259 237 85 60 BspEI T'CCGG_A Cuts at: 5721 5721 Size: 6058 BspHI T'CATG_A Cuts at: 3947 5677 3947 Size: 1730 4328 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2198 2474 3063 3413 844 Size: 1354 276 589 350 3489 Fragments arranged by size: 3489 1354 589 350 276 BsrBI CCG'CTC Cuts at: 1394 1823 3945 1394 Size: 429 2122 3507 Fragments arranged by size: 3507 2122 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2279 4553 4727 65 Size: 1183 1031 2274 174 1396 Fragments arranged by size: 2274 1396 1183 1031 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4702 4889 1700 Size: 110 2892 187 2869 Fragments arranged by size: 2892 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6058 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6058 BssSI C'ACGA_G Cuts at: 3077 4118 5130 3077 Size: 1041 1012 4005 Fragments arranged by size: 4005 1041 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2841 2943 794 Size: 2047 102 3909 Fragments arranged by size: 3909 2047 102 BstBI TT'CG_AA Cuts at: 4862 4862 Size: 6058 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6058 BstXI CCAn_nnnn'nTGG Cuts at: 2290 2290 Size: 6058 BstYI r'GATC_y Cuts at: 1972 2370 2445 2599 3099 3388 3755 4140 4157 Size: 398 75 154 500 289 367 385 17 Cuts at: 4157 5609 5695 5707 6053 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 500 398 385 367 346 289 154 86 75 17 12 Bsu36I CC'TnA_GG Cuts at: 2595 2595 Size: 6058 BtgI C'CryG_G Cuts at: 513 1065 2004 2110 2578 2785 513 Size: 552 939 106 468 207 3786 Fragments arranged by size: 3786 939 552 468 207 106 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5319 Fragments arranged by size: 5319 683 56 BtsI GCAGTG_nn' Cuts at: 2242 3574 4368 4388 2242 Size: 1332 794 20 3912 Fragments arranged by size: 3912 1332 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6058 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6023 DraI TTT'AAA Cuts at: 3362 3362 Size: 6058 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5065 809 Size: 507 3749 1802 Fragments arranged by size: 3749 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2857 2968 3319 Size: 54 1425 50 275 18 1027 111 351 Cuts at: 3319 3505 4394 6023 8 Size: 186 889 1629 43 Fragments arranged by size: 1629 1425 1027 889 351 275 186 111 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3505 1537 Size: 275 1693 4090 Fragments arranged by size: 4090 1693 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2111 2550 3988 1353 Size: 758 439 1438 3423 Fragments arranged by size: 3423 1438 758 439 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2223 4645 5019 5165 387 Size: 1064 138 634 2422 374 146 1280 Fragments arranged by size: 2422 1280 1064 634 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2359 2677 2756 3324 3330 4121 4718 Size: 393 727 318 79 568 6 791 597 Cuts at: 4718 5505 5773 1239 Size: 787 268 1524 Fragments arranged by size: 1524 791 787 727 597 568 393 318 268 79 6 EcoO109I rG'GnC_Cy Cuts at: 2023 2320 3016 2023 Size: 297 696 5065 Fragments arranged by size: 5065 696 297 EcoRI G'AATT_C Cuts at: 3367 3745 3367 Size: 378 5680 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3239 3239 Size: 6058 FspI TGC'GCA Cuts at: 4564 4564 Size: 6058 HaeII r_GCGC'y Cuts at: 1678 2205 2667 5205 1678 Size: 527 462 2538 2531 Fragments arranged by size: 2538 2531 527 462 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4699 4731 4773 4805 1373 Size: 32 135 32 3127 32 42 32 2626 Fragments arranged by size: 3127 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3402 3648 669 Size: 988 281 1464 246 3079 Fragments arranged by size: 3079 1464 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6058 HpaI GTT'AAC Cuts at: 3648 3648 Size: 6058 KasI G'GCGC_C Cuts at: 2201 2201 Size: 6058 KpnI G_GTAC'C Cuts at: 3383 3383 Size: 6058 MfeI C'AATT_G Cuts at: 3657 3657 Size: 6058 MluI A'CGCG_T Cuts at: 5934 5934 Size: 6058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3272 5172 5356 1305 Size: 1967 1900 184 2007 Fragments arranged by size: 2007 1967 1900 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2970 10 Size: 54 1425 343 1138 3098 Fragments arranged by size: 3098 1425 1138 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2272 4016 4375 4534 518 Size: 648 1106 1744 359 159 2042 Fragments arranged by size: 2042 1744 1106 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2430 2500 3474 4151 5299 5544 1606 Size: 824 70 974 677 1148 245 2120 Fragments arranged by size: 2120 1148 974 824 677 245 70 NarI GG'CG_CC Cuts at: 2202 2202 Size: 6058 NcoI C'CATG_G Cuts at: 513 1065 2004 2110 2578 513 Size: 552 939 106 468 3993 Fragments arranged by size: 3993 939 552 468 106 NdeI CA'TA_TG Cuts at: 387 387 Size: 6058 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6058 NotI GC'GGCC_GC Cuts at: 3505 3505 Size: 6058 NspI r_CATG'y Cuts at: 1132 1229 2098 3416 4961 1132 Size: 97 869 1318 1545 2229 Fragments arranged by size: 2229 1545 1318 869 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6058 PciI A'CATG_T Cuts at: 2094 4957 2094 Size: 2863 3195 PflMI CCAn_nnn'nTGG Cuts at: 1108 3302 1108 Size: 2194 3864 PmeI GTTT'AAAC Cuts at: 3362 3362 Size: 6058 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4127 4159 5640 5672 4127 Size: 32 1481 32 4513 Fragments arranged by size: 4513 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2023 3016 2023 Size: 993 5065 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6058 PsiI TTA'TAA Cuts at: 3628 3628 Size: 6058 PspOMI G'GGCC_C Cuts at: 2319 2319 Size: 6058 PspXI vC'TCGA_Gb Cuts at: 2079 2079 Size: 6058 PstI C_TGCA'G Cuts at: 830 2429 3410 830 Size: 1599 981 3478 Fragments arranged by size: 3478 1599 981 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6058 PvuII CAG'CTG Cuts at: 2430 2430 Size: 6058 SalI G'TCGA_C Cuts at: 1936 3400 1936 Size: 1464 4594 SbfI CC_TGCA'GG Cuts at: 3410 3410 Size: 6058 ScaI AGT'ACT Cuts at: 4306 6018 4306 Size: 1712 4346 SexAI A'CCwGG_T Cuts at: 2990 2990 Size: 6058 SfcI C'TryA_G Cuts at: 826 1045 2425 3406 4541 5222 5413 826 Size: 219 1380 981 1135 681 191 1471 Fragments arranged by size: 1471 1380 1135 981 681 219 191 SfoI GGC'GCC Cuts at: 2203 2203 Size: 6058 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6058 SmaI CCC'GGG Cuts at: 1872 2637 3385 1872 Size: 765 748 4545 Fragments arranged by size: 4545 765 748 SmlI C'TyrA_G Cuts at: 820 1017 1943 2079 4162 5063 5325 820 Size: 197 926 136 2083 901 262 1553 Fragments arranged by size: 2083 1553 926 901 262 197 136 SnaBI TAC'GTA Cuts at: 493 3849 493 Size: 3356 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6058 SphI G_CATG'C Cuts at: 1132 3416 1132 Size: 2284 3774 SrfI GCCC'GGGC Cuts at: 2637 2637 Size: 6058 SspI AAT'ATT Cuts at: 5 52 3982 5 Size: 47 3930 2081 Fragments arranged by size: 3930 2081 47 StuI AGG'CCT Cuts at: 1517 2166 2522 3083 1517 Size: 649 356 561 4492 Fragments arranged by size: 4492 649 561 356 StyI C'CwwG_G Cuts at: 513 1065 2004 2110 2578 2700 3000 3164 3497 Size: 552 939 106 468 122 300 164 333 Cuts at: 3497 513 Size: 3074 Fragments arranged by size: 3074 939 552 468 333 300 164 122 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4085 4102 4255 4440 4885 325 Size: 1255 2505 17 153 185 445 1498 Fragments arranged by size: 2505 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2172 4304 6016 Size: 274 80 33 51 1443 195 2132 1712 Cuts at: 6016 96 Size: 138 Fragments arranged by size: 2132 1712 1443 274 195 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3229 3261 3308 3340 6018 6050 3229 Size: 32 47 32 2678 32 3237 Fragments arranged by size: 3237 2678 47 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6058 XbaI T'CTAG_A Cuts at: 3394 3394 Size: 6058 XhoI C'TCGA_G Cuts at: 1943 2079 1943 Size: 136 5922 XmaI C'CCGG_G Cuts at: 1870 2635 3383 1870 Size: 765 748 4545 Fragments arranged by size: 4545 765 748 XmnI GAAnn'nnTTC Cuts at: 3285 4187 3285 Size: 902 5156 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3866 276 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacI SacII SanDI SapI SfiI SwaI XcmI