Restriction map for
pFN21AB7262
(Linear) (Six-base) MAPSORT of: KIBB7262.seq Check: 2697 from: 1 to: 1353
KIBB7262.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 672 751 1325 1353
Size: 354 318 79 574 28
Fragments arranged by size:
574 354 318 79 28
AflIII A'CryG_T
Cuts at: 0 89 1353
Size: 89 1264
AlwNI CAG_nnn'CTG
Cuts at: 0 961 1297 1353
Size: 961 336 56
Fragments arranged by size:
961 336 56
ApaI G_GGCC'C
Cuts at: 0 318 1353
Size: 318 1035
AvaI C'yCGr_G
Cuts at: 0 7 74 630 907 1353
Size: 7 67 556 277 446
Fragments arranged by size:
556 446 277 67 7
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1013 1046 1353
Size: 1013 33 307
Fragments arranged by size:
1013 307 33
BamHI G'GATC_C
Cuts at: 0 594 1353
Size: 594 759
BanI G'GyrC_C
Cuts at: 0 137 196 634 794 999 1353
Size: 137 59 438 160 205 354
Fragments arranged by size:
438 354 205 160 137 59
BanII G_rGCy'C
Cuts at: 0 81 318 1353
Size: 81 237 1035
Fragments arranged by size:
1035 237 81
BbeI G_GCGC'C
Cuts at: 0 200 1353
Size: 200 1153
BbsI GAAGACnn'nnnn_
Cuts at: 0 775 1273 1353
Size: 775 498 80
Fragments arranged by size:
775 498 80
BbvCI CC'TCA_GC
Cuts at: 0 655 1353
Size: 655 698
BciVI GTATCCnnnnn_n'
Cuts at: 0 1041 1353
Size: 1041 312
BglII A'GATC_T
Cuts at: 0 365 440 1094 1353
Size: 365 75 654 259
Fragments arranged by size:
654 365 259 75
Bme1580I G_kGCm'C
Cuts at: 0 318 378 637 1353
Size: 318 60 259 716
Fragments arranged by size:
716 318 259 60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 605 1353
Size: 605 748
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1319 1353
Size: 1319 34
Bpu10I CC'TnA_GC
Cuts at: 0 655 1353
Size: 655 698
BsaHI Gr'CG_yC
Cuts at: 0 197 1353
Size: 197 1156
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 15 1353
Size: 15 1338
BseYI C'CCAG_C
Cuts at: 0 33 245 480 613 639 919 1353
Size: 33 212 235 133 26 280 434
Fragments arranged by size:
434 280 235 212 133 33 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 583 1353
Size: 411 172 770
Fragments arranged by size:
770 411 172
Bsp1286I G_dGCh'C
Cuts at: 0 81 318 378 637 1353
Size: 81 237 60 259 716
Fragments arranged by size:
716 259 237 81 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 193 469 1058 1353
Size: 193 276 589 295
Fragments arranged by size:
589 295 276 193
BsrDI GCAATG_nn'
Cuts at: 0 274 1353
Size: 274 1079
BssSI C'ACGA_G
Cuts at: 0 1072 1353
Size: 1072 281
BstAPI GCAn_nnn'nTGC
Cuts at: 0 836 938 1353
Size: 836 102 415
Fragments arranged by size:
836 415 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 285 1353
Size: 285 1068
BstYI r'GATC_y
Cuts at: 0 365 440 594 1094 1353
Size: 365 75 154 500 259
Fragments arranged by size:
500 365 259 154 75
Bsu36I CC'TnA_GG
Cuts at: 0 590 1353
Size: 590 763
BtgI C'CryG_G
Cuts at: 0 105 573 780 1353
Size: 105 468 207 573
Fragments arranged by size:
573 468 207 105
BtsI GCAGTG_nn'
Cuts at: 0 237 1353
Size: 237 1116
EaeI y'GGCC_r
Cuts at: 0 852 963 1314 1353
Size: 852 111 351 39
Fragments arranged by size:
852 351 111 39
EarI CTCTTCn'nnn_
Cuts at: 0 106 545 1353
Size: 106 439 808
Fragments arranged by size:
808 439 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 218 1353
Size: 218 1135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 672 751 1319 1325 1353
Size: 354 318 79 568 6 28
Fragments arranged by size:
568 354 318 79 28 6
EcoO109I rG'GnC_Cy
Cuts at: 0 18 315 1011 1353
Size: 18 297 696 342
Fragments arranged by size:
696 342 297 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1234 1353
Size: 1234 119
HaeII r_GCGC'y
Cuts at: 0 200 662 1353
Size: 200 462 691
Fragments arranged by size:
691 462 200
KasI G'GCGC_C
Cuts at: 0 196 1353
Size: 196 1157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1267 1353
Size: 1267 86
MscI TGG'CCA
Cuts at: 0 965 1353
Size: 965 388
MslI CAynn'nnrTG
Cuts at: 0 267 1353
Size: 267 1086
MspA1I CmG'CkG
Cuts at: 0 425 495 1353
Size: 425 70 858
Fragments arranged by size:
858 425 70
NarI GG'CG_CC
Cuts at: 0 197 1353
Size: 197 1156
NcoI C'CATG_G
Cuts at: 0 105 573 1353
Size: 105 468 780
Fragments arranged by size:
780 468 105
NspI r_CATG'y
Cuts at: 0 93 1353
Size: 93 1260
PciI A'CATG_T
Cuts at: 0 89 1353
Size: 89 1264
PflMI CCAn_nnn'nTGG
Cuts at: 0 1297 1353
Size: 1297 56
PpuMI rG'GwC_Cy
Cuts at: 0 18 1011 1353
Size: 18 993 342
Fragments arranged by size:
993 342 18
PspOMI G'GGCC_C
Cuts at: 0 314 1353
Size: 314 1039
PspXI vC'TCGA_Gb
Cuts at: 0 74 1353
Size: 74 1279
PstI C_TGCA'G
Cuts at: 0 424 1353
Size: 424 929
PvuII CAG'CTG
Cuts at: 0 425 1353
Size: 425 928
SexAI A'CCwGG_T
Cuts at: 0 985 1353
Size: 985 368
SfcI C'TryA_G
Cuts at: 0 420 1353
Size: 420 933
SfoI GGC'GCC
Cuts at: 0 198 1353
Size: 198 1155
SmaI CCC'GGG
Cuts at: 0 632 1353
Size: 632 721
SmlI C'TyrA_G
Cuts at: 0 74 1353
Size: 74 1279
SrfI GCCC'GGGC
Cuts at: 0 632 1353
Size: 632 721
StuI AGG'CCT
Cuts at: 0 161 517 1078 1353
Size: 161 356 561 275
Fragments arranged by size:
561 356 275 161
StyI C'CwwG_G
Cuts at: 0 105 573 695 995 1159 1353
Size: 105 468 122 300 164 194
Fragments arranged by size:
468 300 194 164 122 105
TatI w'GTAC_w
Cuts at: 0 167 1353
Size: 167 1186
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1224 1256 1303 1335 1353
Size: 1224 32 47 32 18
Fragments arranged by size:
1224 47 32 32 18
XhoI C'TCGA_G
Cuts at: 0 74 1353
Size: 74 1279
XmaI C'CCGG_G
Cuts at: 0 630 1353
Size: 630 723
XmnI GAAnn'nnTTC
Cuts at: 0 1280 1353
Size: 1280 73
Enzymes that do cut:
AcuI AflIII AlwNI ApaI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BglII Bme1580I BplI
BpmI Bpu10I BsaHI BseRI BseYI BsgI Bsp1286I BspMI
BsrDI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI
EaeI EarI EciI Eco57MI EcoO109I FalI HaeII KasI
MmeI MscI MslI MspA1I NarI NcoI NspI PciI
PflMI PpuMI PspOMI PspXI PstI PvuII SexAI SfcI
SfoI SmaI SmlI SrfI StuI StyI TatI TstI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BcgI BclI BfrBI BglI BlpI BmgBI
BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PfoI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI
SpeI SphI SspI SwaI TaqII Tth111I XbaI XcmI
ZraI