Restriction map for
pFN21AB7247
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII Acc65I AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AvrII BbvCI BcgI
BciVI BclI BfrI BlnI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HpaI
KpnI MfeI MluI MseI MunI
NaeI NcoI NdeI NgoMIV NheI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI SexAI SfuI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StyI SwaI TfiI Tru9I Tsp509I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 1774 GTCTAC GT^[AC][GT]AC
AccIII 857 TCCGGA T^CCGGA
AflIII 359 ACGTGT A^C[GA][CT]GT
AlwNI 1510 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 472 GGGCCC GGGCC^C
Asp700I 1392 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 27 TGCGCA TGC^GCA
BamHI 365 GGATCC G^GATCC
BbrPI 952 CACGTG CAC^GTG
BglII 1586 AGATCT A^GATCT
BsaAI 952 CACGTG [CT]AC^GT[GA]
BsaBI 1278 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 857 TCCGGA [AT]^CCGG[AT]
BseAI 857 TCCGGA T^CCGGA
BsiEI 173 CGGCCG CG[GA][CT]^CG
BspEI 857 TCCGGA T^CCGGA
DsaI 6 CCGCGG C^C[GA][CT]GG
EagI 173 CGGCCG C^GGCCG
EclXI 173 CGGCCG C^GGCCG
Eco52I 173 CGGCCG C^GGCCG
EcoNI 1764 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 27 TGCGCA TGC^GCA
HincII 1711 GTCAAC GT[CT]^[GA]AC
HindII 1711 GTCAAC GT[CT]^[GA]AC
HindIII 851 AAGCTT A^AGCTT
KspI 6 CCGCGG CCGC^GG
MamI 1278 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 857 TCCGGA T^CCGGA
NotI 172 GCGGCCGC GC^GGCCGC
NspI 1745 GCATGC [GA]CATG^[CT]
PmaCI 952 CACGTG CAC^GTG
PmlI 952 CACGTG CAC^GTG
PpuMI 386 AGGTCCT [GA]G^G[AT]CC[CT]
SacII 6 CCGCGG CCGC^GG
SgrAI 1417 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1579 CTCAAG C^T[CT][GA]AG
SphI 1745 GCATGC GCATG^C
StuI 1761 AGGCCT AGG^CCT
XmnI 1392 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 897 GTAC GT^AC
1110 GTAC
AspI 583 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1213 GACACGGTC
BbeI 307 GGCGCC GGCGC^C
1421 GGCGCC
BpmI 1195 CTGGAG CTGGAG(16/14)
1498 CTGGAG
BsaI 811 GAGACC GGTCTC(1/5)
63 GGTCTC
BseRI 163 GAGGAG GAGGAG(10/8)
1198 GAGGAG
BsmFI 236 GGGAC GGGAC(10/14)
33 GTCCC
BsrDI 1098 CATTGC GCAATG(2/0)
806 GCAATG
BsrFI 239 ACCGGC [GA]^CCGG[CT]
1418 ACCGGC
BstAPI 515 GCACCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1326 GCATGAGCTGC
BstXI 249 CCAGAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1154 CCATGAGTCTGG
Cfr10I 239 ACCGGC [GA]^CCGG[CT]
1418 ACCGGC
HinfI 748 GACTC G^A[ACGT]TC
1158 GAGTC
KasI 307 GGCGCC G^GCGCC
1421 GGCGCC
NarI 307 GGCGCC GG^CGCC
1421 GGCGCC
PflFI 583 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1213 GACACGGTC
PleI 748 GACTC GAGTC(4/5)
1158 GAGTC
PvuII 510 CAGCTG CAG^CTG
577 CAGCTG
RsaI 897 GTAC GT^AC
1110 GTAC
SapI 501 GCTCTTC GCTCTTC(1/4)
1068 GCTCTTC
ScaI 896 AGTACT AGT^ACT
1109 AGTACT
SfiI 1305 GGCCATTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1524 GGCCTCACTGGCC
SfoI 307 GGCGCC GGC^GCC
1421 GGCGCC
Tth111I 583 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1213 GACACGGTC
XcmI 1151 CCACCATGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1184 CCATACACGGGCTGG
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BlpI BmyI Bpu1102I BpuAI
BsaHI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I TspRI TthHB8I XhoII