(Circular) (Six-base) MAPSORT of: pFN21AB7247.seq Check: 3324 from: 1 to: 6493 pFN21AB7247 6493 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3012 3044 3012 Size: 32 6461 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4303 278 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3780 3836 1937 Size: 1843 56 4594 Fragments arranged by size: 4594 1843 56 Acc65I G'GTAC_C Cuts at: 3814 3814 Size: 6493 AclI AA'CG_TT Cuts at: 1563 4620 4993 1563 Size: 3057 373 3063 Fragments arranged by size: 3063 3057 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2145 2408 2875 4556 5940 6208 1632 Size: 513 263 467 1681 1384 268 1917 Fragments arranged by size: 1917 1681 1384 513 467 268 263 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6296 AflIII A'CryG_T Cuts at: 1203 2364 5392 6369 1203 Size: 1161 3028 977 1327 Fragments arranged by size: 3028 1327 1161 977 AgeI A'CCGG_T Cuts at: 5324 5324 Size: 6493 AhdI GACnn_n'nnGTC Cuts at: 5222 5222 Size: 6493 AleI CACnn'nnGTG Cuts at: 1166 2973 1166 Size: 1807 4686 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3076 1184 Size: 1892 4601 AlwNI CAG_nnn'CTG Cuts at: 3520 4236 5808 3520 Size: 716 1572 4205 Fragments arranged by size: 4205 1572 716 ApaI G_GGCC'C Cuts at: 2481 2481 Size: 6493 ApaLI G'TGCA_C Cuts at: 4550 5706 4550 Size: 1156 5337 ApoI r'AATT_y Cuts at: 1493 3802 4028 4180 6289 1493 Size: 2309 226 152 2109 1697 Fragments arranged by size: 2309 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5047 160 Size: 872 4015 1606 Fragments arranged by size: 4015 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 AvaI C'yCGr_G Cuts at: 1870 1943 2019 2275 3449 3818 1870 Size: 73 76 256 1174 369 4545 Fragments arranged by size: 4545 1174 369 256 76 73 BamHI G'GATC_C Cuts at: 2370 3823 4190 2370 Size: 1453 367 4673 Fragments arranged by size: 4673 1453 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2312 2962 2975 3426 3814 Size: 325 217 627 525 650 13 451 388 Cuts at: 3814 5269 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 650 627 525 451 388 325 217 13 BanII G_rGCy'C Cuts at: 1391 2020 2283 2481 3812 1391 Size: 629 263 198 1331 4072 Fragments arranged by size: 4072 1331 629 263 198 BbeI G_GCGC'C Cuts at: 2316 3430 2316 Size: 1114 5379 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2587 2608 3733 928 Size: 971 688 21 1125 3688 Fragments arranged by size: 3688 1125 971 688 21 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4684 4718 1903 Size: 34 2747 34 3678 Fragments arranged by size: 3678 2747 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4385 5595 4385 Size: 1210 5283 BclI T'GATC_A Cuts at: 1548 1548 Size: 6493 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2024 2258 3180 Size: 107 122 71 631 605 352 234 922 Cuts at: 3180 3285 3317 3536 5104 136 Size: 105 32 219 1568 1525 Fragments arranged by size: 1568 1525 922 631 605 352 234 219 122 107 105 71 32 BglII A'GATC_T Cuts at: 3591 6488 3591 Size: 2897 3596 BlpI GC'TnA_GC Cuts at: 2092 2778 3477 3910 2092 Size: 686 699 433 4675 Fragments arranged by size: 4675 699 686 433 Bme1580I G_kGCm'C Cuts at: 1790 2481 2980 3185 3583 4554 5710 1790 Size: 691 499 205 398 971 1156 2573 Fragments arranged by size: 2573 1156 971 691 499 398 205 BmgBI CAC'GTC Cuts at: 1318 2365 1318 Size: 1047 5446 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3284 4224 5182 Size: 416 53 83 399 24 1867 940 958 Cuts at: 5182 442 Size: 1753 Fragments arranged by size: 1867 1753 958 940 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6493 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3221 3524 5153 1239 Size: 1982 303 1629 2579 Fragments arranged by size: 2579 1982 1629 303 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3569 4618 5483 5781 3569 Size: 1049 865 298 4281 Fragments arranged by size: 4281 1049 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2074 2810 5156 882 Size: 617 575 736 2346 2219 Fragments arranged by size: 2346 2219 736 617 575 BsaAI yAC'GTr Cuts at: 493 1204 2959 4284 493 Size: 711 1755 1325 2702 Fragments arranged by size: 2702 1755 1325 711 BsaBI GATnn'nnATC Cuts at: 3287 6487 3287 Size: 3200 3293 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2054 2313 2625 3427 Size: 53 83 186 936 521 259 312 802 Cuts at: 3427 4300 4682 5317 275 Size: 873 382 635 1451 Fragments arranged by size: 1451 936 873 802 635 521 382 312 259 186 83 53 BsaWI w'CCGG_w Cuts at: 2862 4926 5324 5598 5745 6156 2862 Size: 2064 398 274 147 411 3199 Fragments arranged by size: 3199 2064 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3533 3563 3756 3786 1178 Size: 30 165 30 2130 30 193 30 3885 Fragments arranged by size: 3885 2130 193 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2183 3218 1186 Size: 997 1035 4461 Fragments arranged by size: 4461 1035 997 BseYI C'CCAG_C Cuts at: 2043 3031 3508 5696 6192 2043 Size: 988 477 2188 496 2344 Fragments arranged by size: 2344 2188 988 496 477 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2503 2990 3255 3404 1163 Size: 573 767 487 265 149 4252 Fragments arranged by size: 4252 767 573 487 265 149 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2181 3943 4704 5732 1540 Size: 275 186 180 1762 761 1028 2301 Fragments arranged by size: 2301 1762 1028 761 275 186 180 BsiHKAI G_wGCw'C Cuts at: 2457 2508 2643 3075 4554 4639 5710 2457 Size: 51 135 432 1479 85 1071 3240 Fragments arranged by size: 3240 1479 1071 432 135 85 51 BsmI GAATG_Cn' Cuts at: 4004 4097 4004 Size: 93 6400 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2283 2457 2481 2508 2643 2980 Size: 399 230 263 174 24 27 135 337 Cuts at: 2980 3075 3185 3583 3812 4554 4639 5710 1391 Size: 95 110 398 229 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 398 337 263 230 229 174 135 110 95 85 27 24 BspEI T'CCGG_A Cuts at: 2862 6156 2862 Size: 3294 3199 BspHI T'CATG_A Cuts at: 4382 6112 4382 Size: 1730 4763 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2730 3012 3044 3848 844 Size: 1886 282 32 804 3489 Fragments arranged by size: 3489 1886 804 282 32 BsrBI CCG'CTC Cuts at: 1394 1823 2024 2091 2177 4380 1394 Size: 429 201 67 86 2203 3507 Fragments arranged by size: 3507 2203 429 201 86 67 BsrDI GCAATG_nn' Cuts at: 65 1248 2818 3102 4988 5162 65 Size: 1183 1570 284 1886 174 1396 Fragments arranged by size: 1886 1570 1396 1183 284 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2244 3423 5137 5324 1700 Size: 110 434 1179 1714 187 2869 Fragments arranged by size: 2869 1714 1179 434 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6493 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6493 BssSI C'ACGA_G Cuts at: 4553 5565 4553 Size: 1012 5481 BstAPI GCAn_nnn'nTGC Cuts at: 794 2526 3337 794 Size: 1732 811 3950 Fragments arranged by size: 3950 1732 811 BstBI TT'CG_AA Cuts at: 5297 5297 Size: 6493 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6493 BstXI CCAn_nnnn'nTGG Cuts at: 2261 3166 2261 Size: 905 5588 BstYI r'GATC_y Cuts at: 1972 2370 2464 3591 3823 4190 4575 4592 6044 Size: 398 94 1127 232 367 385 17 1452 Cuts at: 6044 6130 6142 6488 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1127 398 385 367 346 232 94 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2011 513 Size: 552 939 7 4995 Fragments arranged by size: 4995 939 552 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3274 3373 487 Size: 683 56 2048 99 3607 Fragments arranged by size: 3607 2048 683 99 56 BtsI GCAGTG_nn' Cuts at: 2650 4009 4803 4823 2650 Size: 1359 794 20 4320 Fragments arranged by size: 4320 1359 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6493 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6458 DraI TTT'AAA Cuts at: 3797 3797 Size: 6493 DraIII CAC_nnn'GTG Cuts at: 2452 2691 2850 3627 3760 2452 Size: 239 159 777 133 5185 Fragments arranged by size: 5185 777 239 159 133 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5500 809 Size: 507 4184 1802 Fragments arranged by size: 4184 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 2346 2379 Size: 54 1425 50 275 18 348 168 33 Cuts at: 2379 2712 2739 3309 3318 3940 4829 6458 8 Size: 333 27 570 9 622 889 1629 43 Fragments arranged by size: 1629 1425 889 622 570 348 333 275 168 54 50 43 33 27 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2178 3940 1537 Size: 275 366 1762 4090 Fragments arranged by size: 4090 1762 366 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2276 2513 3080 3407 3605 4423 1353 Size: 923 237 567 327 198 818 3423 Fragments arranged by size: 3423 923 818 567 327 237 198 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5080 5454 5600 387 Size: 1064 138 3491 374 146 1280 Fragments arranged by size: 3491 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2145 2408 2875 3221 3524 4556 5153 Size: 393 513 263 467 346 303 1032 597 Cuts at: 5153 5940 6208 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1032 787 597 513 467 393 346 303 268 263 EcoNI CCTnn'n_nnAGG Cuts at: 3773 3773 Size: 6493 EcoO109I rG'GnC_Cy Cuts at: 2392 2477 2478 3415 3454 3517 2392 Size: 85 1 937 39 63 5368 Fragments arranged by size: 5368 937 85 63 39 1 EcoRI G'AATT_C Cuts at: 3802 4180 3802 Size: 378 6115 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2798 2798 Size: 6493 FspI TGC'GCA Cuts at: 2034 4999 2034 Size: 2965 3528 HaeII r_GCGC'y Cuts at: 1678 2099 2316 3328 3430 5640 1678 Size: 421 217 1012 102 2210 2531 Fragments arranged by size: 2531 2210 1012 421 217 102 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2070 2102 5134 5166 5208 Size: 32 135 32 498 32 3032 32 42 Cuts at: 5208 5240 1373 Size: 32 2626 Fragments arranged by size: 3032 2626 498 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3718 3837 4083 669 Size: 988 281 1780 119 246 3079 Fragments arranged by size: 3079 1780 988 281 246 119 HindIII A'AGCT_T Cuts at: 748 2856 748 Size: 2108 4385 HpaI GTT'AAC Cuts at: 4083 4083 Size: 6493 KasI G'GCGC_C Cuts at: 2312 3426 2312 Size: 1114 5379 KpnI G_GTAC'C Cuts at: 3818 3818 Size: 6493 MfeI C'AATT_G Cuts at: 4092 4092 Size: 6493 MluI A'CGCG_T Cuts at: 6369 6369 Size: 6493 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5607 5791 1305 Size: 4302 184 2007 Fragments arranged by size: 4302 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2381 2714 3311 3320 10 Size: 54 1425 343 549 333 597 9 3183 Fragments arranged by size: 3183 1425 597 549 343 333 54 9 MslI CAynn'nnrTG Cuts at: 518 1166 2609 2792 2973 4451 4810 4969 518 Size: 648 1443 183 181 1478 359 159 2042 Fragments arranged by size: 2042 1478 1443 648 359 183 181 159 MspA1I CmG'CkG Cuts at: 1606 2013 2043 2517 2571 2584 3028 3526 3909 Size: 407 30 474 54 13 444 498 383 Cuts at: 3909 4586 5734 5979 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 498 474 444 407 383 245 54 30 13 NarI GG'CG_CC Cuts at: 2313 3427 2313 Size: 1114 5379 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5002 Fragments arranged by size: 5002 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6493 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6493 NotI GC'GGCC_GC Cuts at: 2178 3940 2178 Size: 1762 4731 NspI r_CATG'y Cuts at: 1132 1229 3754 3851 5396 1132 Size: 97 2525 97 1545 2229 Fragments arranged by size: 2525 2229 1545 97 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6493 PciI A'CATG_T Cuts at: 5392 5392 Size: 6493 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6493 PmeI GTTT'AAAC Cuts at: 3797 3797 Size: 6493 PmlI CAC'GTG Cuts at: 2959 2959 Size: 6493 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4562 4594 6075 6107 4562 Size: 32 1481 32 4948 Fragments arranged by size: 4948 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2392 2392 Size: 6493 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6493 PsiI TTA'TAA Cuts at: 4063 4063 Size: 6493 PspOMI G'GGCC_C Cuts at: 2477 2477 Size: 6493 PstI C_TGCA'G Cuts at: 830 2327 3384 3525 3845 830 Size: 1497 1057 141 320 3478 Fragments arranged by size: 3478 1497 1057 320 141 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6493 PvuII CAG'CTG Cuts at: 2517 2584 2517 Size: 67 6426 SacII CC_GC'GG Cuts at: 2014 2014 Size: 6493 SalI G'TCGA_C Cuts at: 1936 3835 1936 Size: 1899 4594 SapI GCTCTTCn'nnn_ Cuts at: 2513 3080 2513 Size: 567 5926 SbfI CC_TGCA'GG Cuts at: 3845 3845 Size: 6493 ScaI AGT'ACT Cuts at: 2903 3116 4741 6453 2903 Size: 213 1625 1712 2943 Fragments arranged by size: 2943 1712 1625 213 SfcI C'TryA_G Cuts at: 826 1045 2323 3380 3521 3553 3841 4976 5657 Size: 219 1278 1057 141 32 288 1135 681 Cuts at: 5657 5848 826 Size: 191 1471 Fragments arranged by size: 1471 1278 1135 1057 681 288 219 191 141 32 SfiI GGCCn_nnn'nGGCC Cuts at: 3317 3536 3317 Size: 219 6274 SfoI GGC'GCC Cuts at: 2314 3428 2314 Size: 1114 5379 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 SgrAI Cr'CCGG_yG Cuts at: 1700 3423 1700 Size: 1723 4770 SmaI CCC'GGG Cuts at: 1872 3820 1872 Size: 1948 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3584 4597 5498 5760 820 Size: 197 926 1641 1013 901 262 1553 Fragments arranged by size: 1641 1553 1013 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4284 493 Size: 3791 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6493 SphI G_CATG'C Cuts at: 1132 3754 3851 1132 Size: 2622 97 3774 Fragments arranged by size: 3774 2622 97 SspI AAT'ATT Cuts at: 5 52 4417 5 Size: 47 4365 2081 Fragments arranged by size: 4365 2081 47 StuI AGG'CCT Cuts at: 1517 3768 1517 Size: 2251 4242 StyI C'CwwG_G Cuts at: 513 1065 2004 3932 513 Size: 552 939 1928 3074 Fragments arranged by size: 3074 1928 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2056 2297 2348 2476 2626 2963 3254 Size: 1255 476 241 51 128 150 337 291 Cuts at: 3254 4520 4537 4690 4875 5320 325 Size: 1266 17 153 185 445 1498 Fragments arranged by size: 1498 1266 1255 476 445 337 291 241 185 153 150 128 51 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2901 3114 4739 Size: 274 80 33 51 1443 924 213 1625 Cuts at: 4739 6451 96 Size: 1712 138 Fragments arranged by size: 1712 1625 1443 924 274 213 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3756 3788 6453 6485 3756 Size: 32 2665 32 3764 Fragments arranged by size: 3764 2665 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2591 3221 1316 Size: 1275 630 4588 Fragments arranged by size: 4588 1275 630 XbaI T'CTAG_A Cuts at: 3829 3829 Size: 6493 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3163 3196 3163 Size: 33 6460 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6493 XmaI C'CCGG_G Cuts at: 1870 3818 1870 Size: 1948 4545 XmnI GAAnn'nnTTC Cuts at: 3401 4622 3401 Size: 1221 5272 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4301 276 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SanDI SexAI SrfI SwaI