Restriction map for
pFN21AB7247
(Linear) (Six-base) MAPSORT of: KIBB7247.seq Check: 9436 from: 1 to: 1788
KIBB7247.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1007 1039 1788
Size: 1007 32 749
Fragments arranged by size:
1007 749 32
AccI GT'mk_AC
Cuts at: 0 1775 1788
Size: 1775 13
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 403 870 1788
Size: 140 263 467 918
Fragments arranged by size:
918 467 263 140
AflIII A'CryG_T
Cuts at: 0 359 1788
Size: 359 1429
AleI CACnn'nnGTG
Cuts at: 0 968 1788
Size: 968 820
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1071 1788
Size: 1071 717
AlwNI CAG_nnn'CTG
Cuts at: 0 1515 1788
Size: 1515 273
ApaI G_GGCC'C
Cuts at: 0 476 1788
Size: 476 1312
AvaI C'yCGr_G
Cuts at: 0 14 270 1444 1788
Size: 14 256 1174 344
Fragments arranged by size:
1174 344 256 14
BamHI G'GATC_C
Cuts at: 0 365 1788
Size: 365 1423
BanI G'GyrC_C
Cuts at: 0 307 957 970 1421 1788
Size: 307 650 13 451 367
Fragments arranged by size:
650 451 367 307 13
BanII G_rGCy'C
Cuts at: 0 15 278 476 1788
Size: 15 263 198 1312
Fragments arranged by size:
1312 263 198 15
BbeI G_GCGC'C
Cuts at: 0 311 1425 1788
Size: 311 1114 363
Fragments arranged by size:
1114 363 311
BbsI GAAGACnn'nnnn_
Cuts at: 0 582 603 1728 1788
Size: 582 21 1125 60
Fragments arranged by size:
1125 582 60 21
BglI GCCn_nnn'nGGC
Cuts at: 0 19 253 1175 1280 1312 1531 1788
Size: 19 234 922 105 32 219 257
Fragments arranged by size:
922 257 234 219 105 32 19
BglII A'GATC_T
Cuts at: 0 1586 1788
Size: 1586 202
BlpI GC'TnA_GC
Cuts at: 0 87 773 1472 1788
Size: 87 686 699 316
Fragments arranged by size:
699 686 316 87
Bme1580I G_kGCm'C
Cuts at: 0 476 975 1180 1578 1788
Size: 476 499 205 398 210
Fragments arranged by size:
499 476 398 210 205
BmgBI CAC'GTC
Cuts at: 0 360 1788
Size: 360 1428
BmrI ACTGGGnnnn_n'
Cuts at: 0 1279 1788
Size: 1279 509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1216 1519 1788
Size: 1216 303 269
Fragments arranged by size:
1216 303 269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1564 1788
Size: 1564 224
BsaI GGTCTCn'nnnn_
Cuts at: 0 69 805 1788
Size: 69 736 983
Fragments arranged by size:
983 736 69
BsaAI yAC'GTr
Cuts at: 0 954 1788
Size: 954 834
BsaBI GATnn'nnATC
Cuts at: 0 1282 1788
Size: 1282 506
BsaHI Gr'CG_yC
Cuts at: 0 49 308 620 1422 1788
Size: 49 259 312 802 366
Fragments arranged by size:
802 366 312 259 49
BsaWI w'CCGG_w
Cuts at: 0 857 1788
Size: 857 931
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1528 1558 1751 1781 1788
Size: 1528 30 193 30 7
Fragments arranged by size:
1528 193 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 1213 1788
Size: 178 1035 575
Fragments arranged by size:
1035 575 178
BseYI C'CCAG_C
Cuts at: 0 38 1026 1503 1788
Size: 38 988 477 285
Fragments arranged by size:
988 477 285 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 498 985 1250 1399 1788
Size: 498 487 265 149 389
Fragments arranged by size:
498 487 389 265 149
BsiEI CG_ry'CG
Cuts at: 0 176 1788
Size: 176 1612
BsiHKAI G_wGCw'C
Cuts at: 0 452 503 638 1070 1788
Size: 452 51 135 432 718
Fragments arranged by size:
718 452 432 135 51
Bsp1286I G_dGCh'C
Cuts at: 0 15 278 452 476 503 638 975 1070
Size: 15 263 174 24 27 135 337 95
Cuts at: 1070 1180 1578 1788
Size: 110 398 210
Fragments arranged by size:
398 337 263 210 174 135 110 95
27 24 15
BspEI T'CCGG_A
Cuts at: 0 857 1788
Size: 857 931
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 725 1007 1039 1788
Size: 725 282 32 749
Fragments arranged by size:
749 725 282 32
BsrBI CCG'CTC
Cuts at: 0 19 86 172 1788
Size: 19 67 86 1616
Fragments arranged by size:
1616 86 67 19
BsrDI GCAATG_nn'
Cuts at: 0 813 1097 1788
Size: 813 284 691
Fragments arranged by size:
813 691 284
BsrFI r'CCGG_y
Cuts at: 0 239 1418 1788
Size: 239 1179 370
Fragments arranged by size:
1179 370 239
BstAPI GCAn_nnn'nTGC
Cuts at: 0 521 1332 1788
Size: 521 811 456
Fragments arranged by size:
811 521 456
BstXI CCAn_nnnn'nTGG
Cuts at: 0 256 1161 1788
Size: 256 905 627
Fragments arranged by size:
905 627 256
BstYI r'GATC_y
Cuts at: 0 365 459 1586 1788
Size: 365 94 1127 202
Fragments arranged by size:
1127 365 202 94
BtgI C'CryG_G
Cuts at: 0 6 1788
Size: 6 1782
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1269 1368 1788
Size: 1269 99 420
Fragments arranged by size:
1269 420 99
BtsI GCAGTG_nn'
Cuts at: 0 645 1788
Size: 645 1143
DraIII CAC_nnn'GTG
Cuts at: 0 447 686 845 1622 1755 1788
Size: 447 239 159 777 133 33
Fragments arranged by size:
777 447 239 159 133 33
EaeI y'GGCC_r
Cuts at: 0 173 341 374 707 734 1304 1313 1788
Size: 173 168 33 333 27 570 9 475
Fragments arranged by size:
570 475 333 173 168 33 27 9
EagI C'GGCC_G
Cuts at: 0 173 1788
Size: 173 1615
EarI CTCTTCn'nnn_
Cuts at: 0 271 508 1075 1402 1600 1788
Size: 271 237 567 327 198 188
Fragments arranged by size:
567 327 271 237 198 188
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 403 870 1216 1519 1788
Size: 140 263 467 346 303 269
Fragments arranged by size:
467 346 303 269 263 140
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1768 1788
Size: 1768 20
EcoO109I rG'GnC_Cy
Cuts at: 0 387 472 473 1410 1449 1512 1788
Size: 387 85 1 937 39 63 276
Fragments arranged by size:
937 387 276 85 63 39 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 793 1788
Size: 793 995
FspI TGC'GCA
Cuts at: 0 29 1788
Size: 29 1759
HaeII r_GCGC'y
Cuts at: 0 94 311 1323 1425 1788
Size: 94 217 1012 102 363
Fragments arranged by size:
1012 363 217 102 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 65 97 1788
Size: 65 32 1691
Fragments arranged by size:
1691 65 32
HincII GTy'rAC
Cuts at: 0 1713 1788
Size: 1713 75
HindIII A'AGCT_T
Cuts at: 0 851 1788
Size: 851 937
KasI G'GCGC_C
Cuts at: 0 307 1421 1788
Size: 307 1114 367
Fragments arranged by size:
1114 367 307
MscI TGG'CCA
Cuts at: 0 376 709 1306 1315 1788
Size: 376 333 597 9 473
Fragments arranged by size:
597 473 376 333 9
MslI CAynn'nnrTG
Cuts at: 0 604 787 968 1788
Size: 604 183 181 820
Fragments arranged by size:
820 604 183 181
MspA1I CmG'CkG
Cuts at: 0 8 38 512 566 579 1023 1521 1788
Size: 8 30 474 54 13 444 498 267
Fragments arranged by size:
498 474 444 267 54 30 13 8
NarI GG'CG_CC
Cuts at: 0 308 1422 1788
Size: 308 1114 366
Fragments arranged by size:
1114 366 308
NotI GC'GGCC_GC
Cuts at: 0 173 1788
Size: 173 1615
NspI r_CATG'y
Cuts at: 0 1749 1788
Size: 1749 39
PmlI CAC'GTG
Cuts at: 0 954 1788
Size: 954 834
PpuMI rG'GwC_Cy
Cuts at: 0 387 1788
Size: 387 1401
PspOMI G'GGCC_C
Cuts at: 0 472 1788
Size: 472 1316
PstI C_TGCA'G
Cuts at: 0 322 1379 1520 1788
Size: 322 1057 141 268
Fragments arranged by size:
1057 322 268 141
PvuII CAG'CTG
Cuts at: 0 512 579 1788
Size: 512 67 1209
Fragments arranged by size:
1209 512 67
SacII CC_GC'GG
Cuts at: 0 9 1788
Size: 9 1779
SapI GCTCTTCn'nnn_
Cuts at: 0 508 1075 1788
Size: 508 567 713
Fragments arranged by size:
713 567 508
ScaI AGT'ACT
Cuts at: 0 898 1111 1788
Size: 898 213 677
Fragments arranged by size:
898 677 213
SfcI C'TryA_G
Cuts at: 0 318 1375 1516 1548 1788
Size: 318 1057 141 32 240
Fragments arranged by size:
1057 318 240 141 32
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1312 1531 1788
Size: 1312 219 257
Fragments arranged by size:
1312 257 219
SfoI GGC'GCC
Cuts at: 0 309 1423 1788
Size: 309 1114 365
Fragments arranged by size:
1114 365 309
SgrAI Cr'CCGG_yG
Cuts at: 0 1418 1788
Size: 1418 370
SmlI C'TyrA_G
Cuts at: 0 1579 1788
Size: 1579 209
SphI G_CATG'C
Cuts at: 0 1749 1788
Size: 1749 39
StuI AGG'CCT
Cuts at: 0 1763 1788
Size: 1763 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 51 292 343 471 621 958 1249 1788
Size: 51 241 51 128 150 337 291 539
Fragments arranged by size:
539 337 291 241 150 128 51 51
TatI w'GTAC_w
Cuts at: 0 896 1109 1788
Size: 896 213 679
Fragments arranged by size:
896 679 213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1751 1783 1788
Size: 1751 32 5
Fragments arranged by size:
1751 32 5
Tth111I GACn'n_nGTC
Cuts at: 0 586 1216 1788
Size: 586 630 572
Fragments arranged by size:
630 586 572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1158 1191 1788
Size: 1158 33 597
Fragments arranged by size:
1158 597 33
XmnI GAAnn'nnTTC
Cuts at: 0 1396 1788
Size: 1396 392
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI
AvaI BamHI BanI BanII BbeI BbsI BglI BglII
BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI
BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI
EarI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HincII HindIII KasI MscI MslI MspA1I NarI NotI
NspI PmlI PpuMI PspOMI PstI PvuII SacII SapI
ScaI SfcI SfiI SfoI SgrAI SmlI SphI StuI
TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbvCI
BcgI BciVI BclI BfrBI BmtI BplI Bpu10I BsiWI
BsmI BsmBI BspHI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DrdI EciI EcoICRI
EcoRI EcoRV FseI FspAI HpaI KpnI MfeI MluI
MmeI NaeI NcoI NdeI NgoMIV NheI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SbfI SexAI SgfI SmaI SnaBI SpeI SrfI SspI
StyI SwaI XbaI XhoI XmaI ZraI