(Circular) (Six-base) MAPSORT of: pFN21AB7245.seq Check: 8926 from: 1 to: 5626 pFN21AB7245 5626 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2729 2729 Size: 5626 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3436 278 Size: 53 83 186 936 1900 2468 Fragments arranged by size: 2468 1900 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2106 2969 1937 Size: 169 863 4594 Fragments arranged by size: 4594 863 169 Acc65I G'GTAC_C Cuts at: 2947 2947 Size: 5626 AclI AA'CG_TT Cuts at: 1563 3753 4126 1563 Size: 2190 373 3063 Fragments arranged by size: 3063 2190 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2211 3689 5073 5341 1632 Size: 579 1478 1384 268 1917 Fragments arranged by size: 1917 1478 1384 579 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5429 AflIII A'CryG_T Cuts at: 1203 4525 5502 1203 Size: 3322 977 1327 Fragments arranged by size: 3322 1327 977 AgeI A'CCGG_T Cuts at: 4457 4457 Size: 5626 AhdI GACnn_n'nnGTC Cuts at: 4355 4355 Size: 5626 AleI CACnn'nnGTG Cuts at: 1166 2450 1166 Size: 1284 4342 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5626 AlwNI CAG_nnn'CTG Cuts at: 2234 3369 4941 2234 Size: 1135 1572 2919 Fragments arranged by size: 2919 1572 1135 ApaLI G'TGCA_C Cuts at: 3683 4839 3683 Size: 1156 4470 ApoI r'AATT_y Cuts at: 1493 2935 3161 3313 5422 1493 Size: 1442 226 152 2109 1697 Fragments arranged by size: 2109 1697 1442 226 152 AseI AT'TA_AT Cuts at: 160 1032 4180 160 Size: 872 3148 1606 Fragments arranged by size: 3148 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 AvaI C'yCGr_G Cuts at: 1870 1943 2064 2951 1870 Size: 73 121 887 4545 Fragments arranged by size: 4545 887 121 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2013 2046 2013 Size: 33 5593 BamHI G'GATC_C Cuts at: 2956 3323 2956 Size: 367 5259 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2330 2947 4402 618 Size: 325 217 627 543 617 1455 1842 Fragments arranged by size: 1842 1455 627 617 543 325 217 BanII G_rGCy'C Cuts at: 1391 2055 2073 2945 1391 Size: 664 18 872 4072 Fragments arranged by size: 4072 872 664 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2035 2332 928 Size: 971 136 297 4222 Fragments arranged by size: 4222 971 297 136 BbvCI CC'TCA_GC Cuts at: 2678 2678 Size: 5626 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3817 3851 1903 Size: 34 1880 34 3678 Fragments arranged by size: 3678 1880 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3518 4728 3518 Size: 1210 4416 BclI T'GATC_A Cuts at: 1548 1548 Size: 5626 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4237 136 Size: 107 122 71 631 605 2565 1525 Fragments arranged by size: 2565 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2304 2601 5621 2304 Size: 297 3020 2309 Fragments arranged by size: 3020 2309 297 BlpI GC'TnA_GC Cuts at: 3043 3043 Size: 5626 Bme1580I G_kGCm'C Cuts at: 1790 3687 4843 1790 Size: 1897 1156 2573 Fragments arranged by size: 2573 1897 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5626 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3357 4315 442 Size: 416 53 83 399 24 1940 958 1753 Fragments arranged by size: 1940 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5626 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2609 4286 1239 Size: 818 552 1677 2579 Fragments arranged by size: 2579 1677 818 552 Bpu10I CC'TnA_GC Cuts at: 2678 2678 Size: 5626 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2582 3751 4616 4914 2582 Size: 1169 865 298 3294 Fragments arranged by size: 3294 1169 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4289 882 Size: 617 2790 2219 Fragments arranged by size: 2790 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2025 3417 493 Size: 711 821 1392 2702 Fragments arranged by size: 2702 1392 821 711 BsaBI GATnn'nnATC Cuts at: 5620 5620 Size: 5626 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3433 3815 4450 275 Size: 53 83 186 936 1900 382 635 1451 Fragments arranged by size: 1900 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4059 4457 4731 4878 5289 4059 Size: 398 274 147 411 4396 Fragments arranged by size: 4396 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2448 2478 1178 Size: 30 165 30 1045 30 4326 Fragments arranged by size: 4326 1045 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2249 2633 1186 Size: 1063 384 4179 Fragments arranged by size: 4179 1063 384 BseYI C'CCAG_C Cuts at: 2163 4829 5325 2163 Size: 2666 496 2464 Fragments arranged by size: 2666 2464 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2554 2707 1163 Size: 573 818 153 4082 Fragments arranged by size: 4082 818 573 153 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3076 3837 4865 1540 Size: 275 186 1075 761 1028 2301 Fragments arranged by size: 2301 1075 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2073 3687 3772 4843 2073 Size: 1614 85 1071 2856 Fragments arranged by size: 2856 1614 1071 85 BsmI GAATG_Cn' Cuts at: 3137 3230 3137 Size: 93 5533 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2073 2945 3687 3772 4843 1391 Size: 399 265 18 872 742 85 1071 2174 Fragments arranged by size: 2174 1071 872 742 399 265 85 18 BspEI T'CCGG_A Cuts at: 5289 5289 Size: 5626 BspHI T'CATG_A Cuts at: 2409 2835 3515 5245 2409 Size: 426 680 1730 2790 Fragments arranged by size: 2790 1730 680 426 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2729 2981 844 Size: 1885 252 3489 Fragments arranged by size: 3489 1885 252 BsrBI CCG'CTC Cuts at: 1394 1823 3513 1394 Size: 429 1690 3507 Fragments arranged by size: 3507 1690 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2373 4121 4295 65 Size: 1183 1125 1748 174 1396 Fragments arranged by size: 1748 1396 1183 1125 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2916 4270 4457 1700 Size: 110 1106 1354 187 2869 Fragments arranged by size: 2869 1354 1106 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5626 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5626 BssSI C'ACGA_G Cuts at: 3686 4698 3686 Size: 1012 4614 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5626 BstBI TT'CG_AA Cuts at: 4430 4430 Size: 5626 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5626 BstXI CCAn_nnnn'nTGG Cuts at: 2122 2452 2524 2122 Size: 330 72 5224 Fragments arranged by size: 5224 330 72 BstYI r'GATC_y Cuts at: 1972 2304 2601 2956 3323 3708 3725 5177 5263 Size: 332 297 355 367 385 17 1452 86 Cuts at: 5263 5275 5621 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 355 346 332 297 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2870 2870 Size: 5626 BtgI C'CryG_G Cuts at: 513 1065 2004 2334 513 Size: 552 939 330 3805 Fragments arranged by size: 3805 939 552 330 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4887 Fragments arranged by size: 4887 683 56 BtsI GCAGTG_nn' Cuts at: 2232 2498 2582 2901 3142 3936 3956 2232 Size: 266 84 319 241 794 20 3902 Fragments arranged by size: 3902 794 319 266 241 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5626 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5591 DraI TTT'AAA Cuts at: 2930 2930 Size: 5626 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4633 809 Size: 507 3317 1802 Fragments arranged by size: 3317 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2824 3073 3962 Size: 54 1425 50 275 18 994 249 889 Cuts at: 3962 5591 8 Size: 1629 43 Fragments arranged by size: 1629 1425 994 889 275 249 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3073 1537 Size: 275 1261 4090 Fragments arranged by size: 4090 1261 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2230 3556 1353 Size: 877 1326 3423 Fragments arranged by size: 3423 1326 877 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4213 4587 4733 387 Size: 1064 138 2624 374 146 1280 Fragments arranged by size: 2624 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2071 2071 Size: 5626 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2211 2609 3689 4286 5073 5341 Size: 393 425 154 398 1080 597 787 268 Cuts at: 5341 1239 Size: 1524 Fragments arranged by size: 1524 1080 787 597 425 398 393 268 154 EcoRI G'AATT_C Cuts at: 2935 3313 2935 Size: 378 5248 FspI TGC'GCA Cuts at: 4132 4132 Size: 5626 HaeII r_GCGC'y Cuts at: 1678 4773 1678 Size: 3095 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2246 2278 2448 2480 2671 Size: 32 135 32 674 32 170 32 191 Cuts at: 2671 2703 4267 4299 4341 4373 1373 Size: 32 1564 32 42 32 2626 Fragments arranged by size: 2626 1564 674 191 170 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2970 3216 669 Size: 988 281 1032 246 3079 Fragments arranged by size: 3079 1032 988 281 246 HindIII A'AGCT_T Cuts at: 748 2810 748 Size: 2062 3564 HpaI GTT'AAC Cuts at: 3216 3216 Size: 5626 KpnI G_GTAC'C Cuts at: 2951 2951 Size: 5626 MfeI C'AATT_G Cuts at: 3225 3225 Size: 5626 MluI A'CGCG_T Cuts at: 5502 5502 Size: 5626 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2631 4740 4924 1305 Size: 1326 2109 184 2007 Fragments arranged by size: 2109 2007 1326 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3804 Fragments arranged by size: 3804 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 2147 2177 2408 2450 2669 2738 Size: 648 954 27 30 231 42 219 69 Cuts at: 2738 2834 3584 3943 4102 518 Size: 96 750 359 159 2042 Fragments arranged by size: 2042 954 750 648 359 231 219 159 96 69 42 30 27 MspA1I CmG'CkG Cuts at: 1606 2482 3042 3719 4867 5112 1606 Size: 876 560 677 1148 245 2120 Fragments arranged by size: 2120 1148 876 677 560 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2334 513 Size: 552 939 330 3805 Fragments arranged by size: 3805 939 552 330 NdeI CA'TA_TG Cuts at: 387 387 Size: 5626 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5626 NotI GC'GGCC_GC Cuts at: 3073 3073 Size: 5626 NspI r_CATG'y Cuts at: 1132 1229 2887 2984 4529 1132 Size: 97 1658 97 1545 2229 Fragments arranged by size: 2229 1658 1545 97 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5626 PciI A'CATG_T Cuts at: 4525 4525 Size: 5626 PflMI CCAn_nnn'nTGG Cuts at: 1108 2323 1108 Size: 1215 4411 PmeI GTTT'AAAC Cuts at: 2930 2930 Size: 5626 PmlI CAC'GTG Cuts at: 2025 2025 Size: 5626 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3695 3727 5208 5240 3695 Size: 32 1481 32 4081 Fragments arranged by size: 4081 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5626 PsiI TTA'TAA Cuts at: 3196 3196 Size: 5626 PstI C_TGCA'G Cuts at: 830 2978 830 Size: 2148 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5626 PvuII CAG'CTG Cuts at: 2482 2482 Size: 5626 SacI G_AGCT'C Cuts at: 2073 2073 Size: 5626 SalI G'TCGA_C Cuts at: 1936 2968 1936 Size: 1032 4594 SbfI CC_TGCA'GG Cuts at: 2978 2978 Size: 5626 ScaI AGT'ACT Cuts at: 2556 3874 5586 2556 Size: 1318 1712 2596 Fragments arranged by size: 2596 1712 1318 SfcI C'TryA_G Cuts at: 826 1045 2974 4109 4790 4981 826 Size: 219 1929 1135 681 191 1471 Fragments arranged by size: 1929 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5626 SmaI CCC'GGG Cuts at: 1872 2953 1872 Size: 1081 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2597 3730 4631 4893 820 Size: 197 926 654 1133 901 262 1553 Fragments arranged by size: 1553 1133 926 901 654 262 197 SnaBI TAC'GTA Cuts at: 493 3417 493 Size: 2924 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5626 SphI G_CATG'C Cuts at: 1132 2984 1132 Size: 1852 3774 SspI AAT'ATT Cuts at: 5 52 3550 5 Size: 47 3498 2081 Fragments arranged by size: 3498 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5626 StyI C'CwwG_G Cuts at: 513 1065 2004 2334 3065 513 Size: 552 939 330 731 3074 Fragments arranged by size: 3074 939 731 552 330 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2191 2416 2440 2744 3653 3670 3823 Size: 1255 611 225 24 304 909 17 153 Cuts at: 3823 4008 4453 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 909 611 445 304 225 185 153 24 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2554 3872 Size: 274 80 33 51 1443 204 373 1318 Cuts at: 3872 5584 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1318 373 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5586 5618 5586 Size: 32 5594 Tth111I GACn'n_nGTC Cuts at: 1316 2039 1316 Size: 723 4903 XbaI T'CTAG_A Cuts at: 2962 2962 Size: 5626 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2248 2248 Size: 5626 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5626 XmaI C'CCGG_G Cuts at: 1870 2951 1870 Size: 1081 4545 XmnI GAAnn'nnTTC Cuts at: 3755 3755 Size: 5626 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3434 276 Size: 53 83 186 936 1900 2468 Fragments arranged by size: 2468 1900 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI