Restriction map for
pFN21AB7240
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmAI BsmBI
BsoBI BspDI BspEI BspHI BsrBI
BsrFI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NlaIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 739 GCGG CCGC(-3/-1)
AcsI 508 AAATTT [GA]^AATT[CT]
AlwI 757 GGATC GGATC(4/5)
AlwNI 23 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 873 CCTGAGG CC^T[ACGT]AGG
ApoI 508 AAATTT [GA]^AATT[CT]
AspHI 64 GAGCTC G[AT]GC[AT]^C
BalI 371 TGGCCA TGG^CCA
BbvCI 186 CCTCAGC CCTCAGC(-5/-2)
BciVI 817 GTATCC GTATCC(6/5)
BfaI 569 CTAG C^TAG
BpmI 67 CTCCAG CTGGAG(16/14)
BsgI 163 CTGCAC GTGCAG(16/14)
BsiHKAI 64 GAGCTC G[AT]GC[AT]^C
BsmFI 625 GTCCC GGGAC(10/14)
Bsp1407I 392 TGTACA T^GTACA
BsrDI 349 GCAATG GCAATG(2/0)
BsrGI 392 TGTACA T^GTACA
BstYI 757 GGATCT [GA]^GATC[CT]
Bsu36I 873 CCTGAGG CC^T[ACGT]AGG
DsaI 800 CCATGG C^C[GA][CT]GG
EaeI 371 TGGCCA [CT]^GGCC[GA]
EarI 79 CTCTTC CTCTTC(1/4)
Eco81I 873 CCTGAGG CC^T[ACGT]AGG
EcoT14I 800 CCATGG C^C[AT][AT]GG
EcoT22I 288 ATGCAT ATGCA^T
HincII 580 GTCAAC GT[CT]^[GA]AC
HindII 580 GTCAAC GT[CT]^[GA]AC
Ksp632I 79 CTCTTC CTCTTC(1/4)
MaeI 569 CTAG C^TAG
MflI 757 GGATCT [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MspA1I 70 CAGCTG C[AC]G^C[GT]G
NcoI 800 CCATGG C^CATGG
NsiI 288 ATGCAT ATGCA^T
PflMI 729 CCACATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 294 GACTC GAGTC(4/5)
PstI 761 CTGCAG CTGCA^G
PvuII 70 CAGCTG CAG^CTG
SacI 64 GAGCTC GAGCT^C
SspBI 392 TGTACA T^GTACA
StuI 453 AGGCCT AGG^CCT
StyI 800 CCATGG C^C[AT][AT]GG
TfiI 98 GAATC G^A[AT]TC
Van91I 729 CCACATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 757 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AflIII 338 ACATGT A^C[GA][CT]GT
731 ACATGT
AvaII 701 GGTCC G^G[AT]CC
804 GGACC
BanII 46 GGGCTC G[GA]GC[CT]^C
64 GAGCTC
BsiYI 363 CCGTTACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
729 CCACATGTTGG
BslI 363 CCGTTACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
729 CCACATGTTGG
BsmI 286 GAATGC GAATGC(1/-1)
766 GCATTC
BspLU11I 338 ACATGT A^CATGT
731 ACATGT
BspMI 161 ACCTGC ACCTGC(4/8)
698 GCAGGT
Cac8I 418 GCTTGC GC[ACGT]^[ACGT]GC
667 GCCAGC
Cfr13I 701 GGTCC G^G[ACGT]CC
804 GGACC
DpnII 538 GATC ^GATC
758 GATC
HaeIII 372 GGCC GG^CC
454 GGCC
HinfI 98 GAATC G^A[ACGT]TC
294 GACTC
HphI 246 GGTGA GGTGA(8/7)
159 TCACC
MboI 538 GATC ^GATC
758 GATC
MseI 521 TTAA T^TAA
614 TTAA
MslI 111 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
168 CACCCCTATG
NdeII 538 GATC ^GATC
758 GATC
NspI 338 ACATGT [GA]CATG^[CT]
731 ACATGT
Sau3AI 538 GATC ^GATC
758 GATC
Sau96I 701 GGTCC G^G[ACGT]CC
804 GGACC
Tru9I 521 TTAA T^TAA
614 TTAA
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AfaI AluI BbvI BmyI BsaJI
Bsp1286I BssKI BstF5I BstNI DdeI
EcoRII Fnu4HI FokI ItaI MaeIII
MboII MnlI MvaI MwoI NlaIII
RsaI ScrFI SfaNI SfcI TseI
Tsp45I Tsp509I TspRI