(Circular) (Six-base) MAPSORT of: pFN21AB7237.seq Check: 3884 from: 1 to: 6148 pFN21AB7237 6148 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3958 278 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2210 2400 3491 1937 Size: 273 190 1091 4594 Fragments arranged by size: 4594 1091 273 190 Acc65I G'GTAC_C Cuts at: 3469 3469 Size: 6148 AclI AA'CG_TT Cuts at: 1563 4275 4648 1563 Size: 2712 373 3063 Fragments arranged by size: 3063 2712 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2149 2330 4211 5595 5863 1632 Size: 517 181 1881 1384 268 1917 Fragments arranged by size: 1917 1881 1384 517 268 181 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5951 AflIII A'CryG_T Cuts at: 1203 3428 5047 6024 1203 Size: 2225 1619 977 1327 Fragments arranged by size: 2225 1619 1327 977 AgeI A'CCGG_T Cuts at: 4979 4979 Size: 6148 AhdI GACnn_n'nnGTC Cuts at: 4877 4877 Size: 6148 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6148 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6148 AlwNI CAG_nnn'CTG Cuts at: 3891 5463 3891 Size: 1572 4576 ApaLI G'TGCA_C Cuts at: 2480 4205 5361 2480 Size: 1725 1156 3267 Fragments arranged by size: 3267 1725 1156 ApoI r'AATT_y Cuts at: 1493 2135 2802 3457 3683 3835 5944 1493 Size: 642 667 655 226 152 2109 1697 Fragments arranged by size: 2109 1697 667 655 642 226 152 AseI AT'TA_AT Cuts at: 160 1032 4702 160 Size: 872 3670 1606 Fragments arranged by size: 3670 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 AvaI C'yCGr_G Cuts at: 1870 1943 3473 1870 Size: 73 1530 4545 Fragments arranged by size: 4545 1530 73 BamHI G'GATC_C Cuts at: 3478 3845 3478 Size: 367 5781 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3469 4924 618 Size: 325 217 627 1682 1455 1842 Fragments arranged by size: 1842 1682 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2554 2730 3290 3467 1391 Size: 1163 176 560 177 4072 Fragments arranged by size: 4072 1163 560 177 176 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3025 928 Size: 971 1126 4051 Fragments arranged by size: 4051 1126 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4339 4373 1903 Size: 34 2402 34 3678 Fragments arranged by size: 3678 2402 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2306 3040 4040 5250 2306 Size: 734 1000 1210 3204 Fragments arranged by size: 3204 1210 1000 734 BclI T'GATC_A Cuts at: 1548 1548 Size: 6148 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4759 136 Size: 107 122 71 631 605 3087 1525 Fragments arranged by size: 3087 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6143 6143 Size: 6148 BlpI GC'TnA_GC Cuts at: 2235 3565 2235 Size: 1330 4818 Bme1580I G_kGCm'C Cuts at: 1790 2484 4209 5365 1790 Size: 694 1725 1156 2573 Fragments arranged by size: 2573 1725 1156 694 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6148 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3879 4837 442 Size: 416 53 83 399 24 2462 958 1753 Fragments arranged by size: 2462 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2425 2620 4808 1239 Size: 1186 195 2188 2579 Fragments arranged by size: 2579 2188 1186 195 Bpu10I CC'TnA_GC Cuts at: 2545 2545 Size: 6148 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4273 5138 5436 4273 Size: 865 298 4985 Fragments arranged by size: 4985 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2186 4811 882 Size: 617 687 2625 2219 Fragments arranged by size: 2625 2219 687 617 BsaAI yAC'GTr Cuts at: 493 1204 3939 493 Size: 711 2735 2702 Fragments arranged by size: 2735 2702 711 BsaBI GATnn'nnATC Cuts at: 2748 6142 2748 Size: 3394 2754 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3955 4337 4972 275 Size: 53 83 186 936 2422 382 635 1451 Fragments arranged by size: 2422 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4581 4979 5253 5400 5811 4581 Size: 398 274 147 411 4918 Fragments arranged by size: 4918 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2398 2428 1178 Size: 30 165 30 995 30 4898 Fragments arranged by size: 4898 995 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6148 BseYI C'CCAG_C Cuts at: 2621 2965 5351 5847 2621 Size: 344 2386 496 2922 Fragments arranged by size: 2922 2386 496 344 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5575 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3598 4359 5387 1540 Size: 275 186 1597 761 1028 2301 Fragments arranged by size: 2301 1597 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2053 2484 3290 4209 4294 5365 2053 Size: 431 806 919 85 1071 2836 Fragments arranged by size: 2836 1071 919 806 431 85 BsmI GAATG_Cn' Cuts at: 2718 3659 3752 2718 Size: 941 93 5114 Fragments arranged by size: 5114 941 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 2484 2554 2730 3290 3467 4209 Size: 399 263 431 70 176 560 177 742 Cuts at: 4209 4294 5365 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 560 431 399 263 177 176 85 70 BspEI T'CCGG_A Cuts at: 5811 5811 Size: 6148 BspHI T'CATG_A Cuts at: 4037 5767 4037 Size: 1730 4418 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3366 3503 844 Size: 2522 137 3489 Fragments arranged by size: 3489 2522 137 BsrBI CCG'CTC Cuts at: 1394 1823 4035 1394 Size: 429 2212 3507 Fragments arranged by size: 3507 2212 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4643 4817 65 Size: 1183 3395 174 1396 Fragments arranged by size: 3395 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4792 4979 1700 Size: 110 2982 187 2869 Fragments arranged by size: 2982 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3431 96 Size: 3335 2813 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6148 BssSI C'ACGA_G Cuts at: 4208 5220 4208 Size: 1012 5136 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6148 BstBI TT'CG_AA Cuts at: 4952 4952 Size: 6148 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6148 BstYI r'GATC_y Cuts at: 1972 3321 3478 3845 4230 4247 5699 5785 5797 Size: 1349 157 367 385 17 1452 86 12 Cuts at: 5797 6143 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1349 385 367 346 157 86 17 12 BstZ17I GTA'TAC Cuts at: 2211 2211 Size: 6148 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5596 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5409 Fragments arranged by size: 5409 683 56 BtsI GCAGTG_nn' Cuts at: 3305 3664 4458 4478 3305 Size: 359 794 20 4975 Fragments arranged by size: 4975 794 359 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6148 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2664 2699 529 Size: 35 2100 35 3978 Fragments arranged by size: 3978 2100 35 35 DraI TTT'AAA Cuts at: 2989 3000 3139 3452 2989 Size: 11 139 313 5685 Fragments arranged by size: 5685 313 139 11 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5155 809 Size: 507 3839 1802 Fragments arranged by size: 3839 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3595 4484 6113 Size: 54 1425 50 275 18 1765 889 1629 Cuts at: 6113 8 Size: 43 Fragments arranged by size: 1765 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3595 1537 Size: 275 1783 4090 Fragments arranged by size: 4090 1783 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3162 4078 1353 Size: 1809 916 3423 Fragments arranged by size: 3423 1809 916 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4735 5109 5255 387 Size: 1064 138 3146 374 146 1280 Fragments arranged by size: 3146 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3288 3288 Size: 6148 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2149 2330 2425 2620 4211 4808 5595 Size: 393 517 181 95 195 1591 597 787 Cuts at: 5595 5863 1239 Size: 268 1524 Fragments arranged by size: 1591 1524 787 597 517 393 268 195 181 95 EcoRI G'AATT_C Cuts at: 2135 2802 3457 3835 2135 Size: 667 655 378 4448 Fragments arranged by size: 4448 667 655 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2179 2935 3431 2179 Size: 756 496 4896 Fragments arranged by size: 4896 756 496 FspI TGC'GCA Cuts at: 4654 4654 Size: 6148 HaeII r_GCGC'y Cuts at: 1678 5295 1678 Size: 3617 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3214 3246 3277 3309 4789 Size: 32 135 32 1642 32 31 32 1480 Cuts at: 4789 4821 4863 4895 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1642 1480 135 42 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 2591 3492 3738 669 Size: 988 281 653 901 246 3079 Fragments arranged by size: 3079 988 901 653 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6148 HpaI GTT'AAC Cuts at: 3738 3738 Size: 6148 KpnI G_GTAC'C Cuts at: 3473 3473 Size: 6148 MfeI C'AATT_G Cuts at: 3747 3747 Size: 6148 MluI A'CGCG_T Cuts at: 6024 6024 Size: 6148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2551 5262 5446 1305 Size: 1246 2711 184 2007 Fragments arranged by size: 2711 2007 1246 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4326 Fragments arranged by size: 4326 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 2537 2559 4106 4465 4624 518 Size: 648 1035 336 22 1547 359 159 2042 Fragments arranged by size: 2042 1547 1035 648 359 336 159 22 MspA1I CmG'CkG Cuts at: 1606 3564 4241 5389 5634 1606 Size: 1958 677 1148 245 2120 Fragments arranged by size: 2120 1958 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5596 NdeI CA'TA_TG Cuts at: 387 387 Size: 6148 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6148 NotI GC'GGCC_GC Cuts at: 3595 3595 Size: 6148 NspI r_CATG'y Cuts at: 1132 1229 3312 3432 3506 5051 1132 Size: 97 2083 120 74 1545 2229 Fragments arranged by size: 2229 2083 1545 120 97 74 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6148 PciI A'CATG_T Cuts at: 3428 5047 3428 Size: 1619 4529 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6148 PmeI GTTT'AAAC Cuts at: 3452 3452 Size: 6148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4217 4249 5730 5762 4217 Size: 32 1481 32 4603 Fragments arranged by size: 4603 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6148 PsiI TTA'TAA Cuts at: 2433 3204 3240 3718 2433 Size: 771 36 478 4863 Fragments arranged by size: 4863 771 478 36 PstI C_TGCA'G Cuts at: 830 3300 3500 830 Size: 2470 200 3478 Fragments arranged by size: 3478 2470 200 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6148 SacI G_AGCT'C Cuts at: 3290 3290 Size: 6148 SalI G'TCGA_C Cuts at: 1936 3490 1936 Size: 1554 4594 SapI GCTCTTCn'nnn_ Cuts at: 3162 3162 Size: 6148 SbfI CC_TGCA'GG Cuts at: 3500 3500 Size: 6148 ScaI AGT'ACT Cuts at: 4396 6108 4396 Size: 1712 4436 SfcI C'TryA_G Cuts at: 826 1045 3296 3496 4631 5312 5503 826 Size: 219 2251 200 1135 681 191 1471 Fragments arranged by size: 2251 1471 1135 681 219 200 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6148 SmaI CCC'GGG Cuts at: 1872 3475 1872 Size: 1603 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4252 5153 5415 820 Size: 197 926 2309 901 262 1553 Fragments arranged by size: 2309 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3939 493 Size: 3446 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6148 SphI G_CATG'C Cuts at: 1132 3506 1132 Size: 2374 3774 SspI AAT'ATT Cuts at: 5 52 3256 3385 4072 5 Size: 47 3204 129 687 2081 Fragments arranged by size: 3204 2081 687 129 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6148 StyI C'CwwG_G Cuts at: 513 1065 2627 2730 3173 3587 513 Size: 552 1562 103 443 414 3074 Fragments arranged by size: 3074 1562 552 443 414 103 SwaI ATTT'AAAT Cuts at: 3139 3139 Size: 6148 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2086 4175 4192 4345 4530 4975 325 Size: 1255 506 2089 17 153 185 445 1498 Fragments arranged by size: 2089 1498 1255 506 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3388 3431 4394 Size: 274 80 33 51 1443 1411 43 963 Cuts at: 4394 6106 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1411 963 274 138 80 51 43 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2398 2430 6108 6140 2398 Size: 32 3678 32 2406 Fragments arranged by size: 3678 2406 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6148 XbaI T'CTAG_A Cuts at: 3484 3484 Size: 6148 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3040 3040 Size: 6148 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6148 XmaI C'CCGG_G Cuts at: 1870 3473 1870 Size: 1603 4545 XmnI GAAnn'nnTTC Cuts at: 3411 4277 3411 Size: 866 5282 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3956 276 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI