(Circular) (Six-base) MAPSORT of: pFN21AB7229.seq Check: 6822 from: 1 to: 5824 pFN21AB7229 5824 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2702 2702 Size: 5824 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2574 3167 1937 Size: 637 593 4594 Fragments arranged by size: 4594 637 593 Acc65I G'GTAC_C Cuts at: 3145 3145 Size: 5824 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2314 2723 2740 3887 5271 5539 1632 Size: 682 409 17 1147 1384 268 1917 Fragments arranged by size: 1917 1384 1147 682 409 268 17 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 4723 5700 1203 Size: 3520 977 1327 Fragments arranged by size: 3520 1327 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 4553 4553 Size: 5824 AleI CACnn'nnGTG Cuts at: 1166 2145 2549 3008 1166 Size: 979 404 459 3982 Fragments arranged by size: 3982 979 459 404 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2174 2213 2489 1184 Size: 990 39 276 4519 Fragments arranged by size: 4519 990 276 39 AlwNI CAG_nnn'CTG Cuts at: 3567 5139 3567 Size: 1572 4252 ApaI G_GGCC'C Cuts at: 2075 2075 Size: 5824 ApaLI G'TGCA_C Cuts at: 3881 5037 3881 Size: 1156 4668 ApoI r'AATT_y Cuts at: 1493 3133 3359 3511 5620 1493 Size: 1640 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 2618 3149 1870 Size: 73 675 531 4545 Fragments arranged by size: 4545 675 531 73 BamHI G'GATC_C Cuts at: 3154 3521 3154 Size: 367 5457 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 3145 4600 618 Size: 325 217 627 246 1112 1455 1842 Fragments arranged by size: 1842 1455 1112 627 325 246 217 BanII G_rGCy'C Cuts at: 1391 2075 3143 1391 Size: 684 1068 4072 Fragments arranged by size: 4072 1068 684 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2644 928 Size: 971 745 4108 Fragments arranged by size: 4108 971 745 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4015 4049 1903 Size: 34 2078 34 3678 Fragments arranged by size: 3678 2078 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4435 136 Size: 107 122 71 631 605 2763 1525 Fragments arranged by size: 2763 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 2718 3241 2718 Size: 523 5301 Bme1580I G_kGCm'C Cuts at: 1790 2075 3885 5041 1790 Size: 285 1810 1156 2573 Fragments arranged by size: 2573 1810 1156 285 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5824 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2777 3555 4513 Size: 416 53 83 399 24 1360 778 958 Cuts at: 4513 442 Size: 1753 Fragments arranged by size: 1753 1360 958 778 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2877 2877 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2429 2618 2656 4484 1239 Size: 1190 189 38 1828 2579 Fragments arranged by size: 2579 1828 1190 189 38 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3949 4814 5112 3949 Size: 865 298 4661 Fragments arranged by size: 4661 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2101 2585 4487 882 Size: 617 602 484 1902 2219 Fragments arranged by size: 2219 1902 617 602 484 BsaAI yAC'GTr Cuts at: 493 1204 2473 3615 493 Size: 711 1269 1142 2702 Fragments arranged by size: 2702 1269 1142 711 BsaBI GATnn'nnATC Cuts at: 2261 2687 5818 2261 Size: 426 3131 2267 Fragments arranged by size: 3131 2267 426 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3631 4013 4648 275 Size: 53 83 186 936 2098 382 635 1451 Fragments arranged by size: 2098 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4257 4655 4929 5076 5487 4257 Size: 398 274 147 411 4594 Fragments arranged by size: 4594 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5599 Fragments arranged by size: 5599 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2366 2399 1186 Size: 1180 33 4611 Fragments arranged by size: 4611 1180 33 BseYI C'CCAG_C Cuts at: 3019 5027 5523 3019 Size: 2008 496 3320 Fragments arranged by size: 3320 2008 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5251 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 4035 5063 1540 Size: 275 186 1273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3885 3970 5041 3885 Size: 85 1071 4668 Fragments arranged by size: 4668 1071 85 BsmI GAATG_Cn' Cuts at: 2533 2600 3335 3428 2533 Size: 67 735 93 4929 Fragments arranged by size: 4929 735 93 67 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 3143 3885 3970 5041 1391 Size: 399 285 1068 742 85 1071 2174 Fragments arranged by size: 2174 1071 1068 742 399 285 85 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 2868 3713 5443 2868 Size: 845 1730 3249 Fragments arranged by size: 3249 1730 845 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2152 2702 3179 844 Size: 1308 550 477 3489 Fragments arranged by size: 3489 1308 550 477 BsrBI CCG'CTC Cuts at: 1394 1823 3711 1394 Size: 429 1888 3507 Fragments arranged by size: 3507 1888 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2296 4319 4493 65 Size: 1183 1048 2023 174 1396 Fragments arranged by size: 2023 1396 1183 1048 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4468 4655 1700 Size: 110 2658 187 2869 Fragments arranged by size: 2869 2658 187 110 BsrGI T'GTAC_A Cuts at: 96 2184 96 Size: 2088 3736 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 2141 3884 4896 2141 Size: 1743 1012 3069 Fragments arranged by size: 3069 1743 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5824 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 2707 2966 1184 Size: 1523 259 4042 Fragments arranged by size: 4042 1523 259 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2147 2011 Size: 136 5688 BstYI r'GATC_y Cuts at: 1972 3154 3521 3906 3923 5375 5461 5473 5819 Size: 1182 367 385 17 1452 86 12 346 Cuts at: 5819 1972 Size: 1977 Fragments arranged by size: 1977 1452 1182 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2100 2217 2337 2560 2934 513 Size: 552 939 96 117 120 223 374 3403 Fragments arranged by size: 3403 939 552 374 223 120 117 96 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5085 Fragments arranged by size: 5085 683 56 BtsI GCAGTG_nn' Cuts at: 2477 2497 2990 3093 3340 4134 4154 2477 Size: 20 493 103 247 794 20 4147 Fragments arranged by size: 4147 794 493 247 103 20 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2466 2501 529 Size: 35 1902 35 3852 Fragments arranged by size: 3852 1902 35 35 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DraIII CAC_nnn'GTG Cuts at: 2503 3109 2503 Size: 606 5218 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4831 809 Size: 507 3515 1802 Fragments arranged by size: 3515 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2505 3271 4160 Size: 54 1425 50 275 18 675 766 889 Cuts at: 4160 5789 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 766 675 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2399 2599 3754 1353 Size: 1046 200 1155 3423 Fragments arranged by size: 3423 1155 1046 200 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4411 4785 4931 387 Size: 1064 138 2822 374 146 1280 Fragments arranged by size: 2822 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2314 2429 2618 2656 2723 2740 3887 Size: 393 682 115 189 38 67 17 1147 Cuts at: 3887 4484 5271 5539 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1147 787 682 597 393 268 189 115 67 38 17 EcoO109I rG'GnC_Cy Cuts at: 2409 2725 2409 Size: 316 5508 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 4971 1678 Size: 3293 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2353 2385 2393 2425 4465 Size: 32 135 32 781 32 8 32 2040 Cuts at: 4465 4497 4539 4571 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2040 781 135 42 32 32 32 32 32 32 8 HincII GTy'rAC Cuts at: 669 1657 1938 2575 3168 3414 669 Size: 988 281 637 593 246 3079 Fragments arranged by size: 3079 988 637 593 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 3149 3149 Size: 5824 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2113 2793 4938 5122 1305 Size: 808 680 2145 184 2007 Fragments arranged by size: 2145 2007 808 680 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2507 10 Size: 54 1425 343 675 3327 Fragments arranged by size: 3327 1425 675 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2145 2549 3008 3782 4141 4300 Size: 648 843 136 404 459 774 359 159 Cuts at: 4300 518 Size: 2042 Fragments arranged by size: 2042 843 774 648 459 404 359 159 136 MspA1I CmG'CkG Cuts at: 1606 2775 3240 3917 5065 5310 1606 Size: 1169 465 677 1148 245 2120 Fragments arranged by size: 2120 1169 1148 677 465 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2100 2560 513 Size: 552 939 96 460 3777 Fragments arranged by size: 3777 939 552 460 96 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 2161 2912 3182 4727 1132 Size: 97 932 751 270 1545 2229 Fragments arranged by size: 2229 1545 932 751 270 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5824 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 2503 2775 1108 Size: 1395 272 4157 Fragments arranged by size: 4157 1395 272 PfoI T'CCnGG_A Cuts at: 2849 2952 2849 Size: 103 5721 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PmlI CAC'GTG Cuts at: 2473 2473 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3893 3925 5406 5438 3893 Size: 32 1481 32 4279 Fragments arranged by size: 4279 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2409 2725 2409 Size: 316 5508 PshAI GACnn'nnGTC Cuts at: 1613 2805 1613 Size: 1192 4632 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PspOMI G'GGCC_C Cuts at: 2071 2071 Size: 5824 PstI C_TGCA'G Cuts at: 830 3176 830 Size: 2346 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 PvuII CAG'CTG Cuts at: 2775 2775 Size: 5824 SalI G'TCGA_C Cuts at: 1936 2573 3166 1936 Size: 637 593 4594 Fragments arranged by size: 4594 637 593 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SexAI A'CCwGG_T Cuts at: 2318 2318 Size: 5824 SfcI C'TryA_G Cuts at: 826 1045 3172 4307 4988 5179 826 Size: 219 2127 1135 681 191 1471 Fragments arranged by size: 2127 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 2620 3151 1872 Size: 748 531 4545 Fragments arranged by size: 4545 748 531 SmlI C'TyrA_G Cuts at: 820 1017 1943 3928 4829 5091 820 Size: 197 926 1985 901 262 1553 Fragments arranged by size: 1985 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 2161 3182 1132 Size: 1029 1021 3774 Fragments arranged by size: 3774 1029 1021 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 2081 2747 3030 1517 Size: 564 666 283 4311 Fragments arranged by size: 4311 666 564 283 StyI C'CwwG_G Cuts at: 513 1065 2004 2067 2100 2560 3263 513 Size: 552 939 63 33 460 703 3074 Fragments arranged by size: 3074 939 703 552 460 63 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3851 3868 4021 4206 4651 325 Size: 1255 2271 17 153 185 445 1498 Fragments arranged by size: 2271 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 4070 5782 Size: 274 80 33 51 1443 207 1886 1712 Cuts at: 5782 96 Size: 138 Fragments arranged by size: 1886 1712 1443 274 207 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2934 2966 5784 5816 2934 Size: 32 2818 32 2942 Fragments arranged by size: 2942 2818 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2422 2422 Size: 5824 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 2618 3149 1870 Size: 748 531 4545 Fragments arranged by size: 4545 748 531 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI