(Circular) (Six-base) MAPSORT of: pFN21AB7216.seq Check: 7716 from: 1 to: 5152 pFN21AB7216 5152 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2962 278 Size: 53 83 186 936 1426 2468 Fragments arranged by size: 2468 1426 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2495 1937 Size: 558 4594 Acc65I G'GTAC_C Cuts at: 2473 2473 Size: 5152 AclI AA'CG_TT Cuts at: 1563 3279 3652 1563 Size: 1716 373 3063 Fragments arranged by size: 3063 1716 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3215 4599 4867 1632 Size: 1583 1384 268 1917 Fragments arranged by size: 1917 1583 1384 268 AfeI AGC'GCT Cuts at: 2016 2016 Size: 5152 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4955 AflIII A'CryG_T Cuts at: 1203 4051 5028 1203 Size: 2848 977 1327 Fragments arranged by size: 2848 1327 977 AgeI A'CCGG_T Cuts at: 3983 3983 Size: 5152 AhdI GACnn_n'nnGTC Cuts at: 2078 3881 2078 Size: 1803 3349 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5152 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2081 1184 Size: 897 4255 AlwNI CAG_nnn'CTG Cuts at: 2895 4467 2895 Size: 1572 3580 ApaI G_GGCC'C Cuts at: 2211 2211 Size: 5152 ApaLI G'TGCA_C Cuts at: 3209 4365 3209 Size: 1156 3996 ApoI r'AATT_y Cuts at: 1493 2461 2687 2839 4948 1493 Size: 968 226 152 2109 1697 Fragments arranged by size: 2109 1697 968 226 152 AseI AT'TA_AT Cuts at: 160 1032 3706 160 Size: 872 2674 1606 Fragments arranged by size: 2674 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5152 AvaI C'yCGr_G Cuts at: 1870 1943 2183 2477 1870 Size: 73 240 294 4545 Fragments arranged by size: 4545 294 240 73 AvrII C'CTAG_G Cuts at: 2116 2116 Size: 5152 BamHI G'GATC_C Cuts at: 2482 2849 2482 Size: 367 4785 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2120 2170 2473 3928 618 Size: 325 217 627 333 50 303 1455 1842 Fragments arranged by size: 1842 1455 627 333 325 303 217 50 BanII G_rGCy'C Cuts at: 1391 2211 2268 2399 2471 1391 Size: 820 57 131 72 4072 Fragments arranged by size: 4072 820 131 72 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4181 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3343 3377 1903 Size: 34 1406 34 3678 Fragments arranged by size: 3678 1406 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3044 4254 3044 Size: 1210 3942 BclI T'GATC_A Cuts at: 1548 1548 Size: 5152 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3763 136 Size: 107 122 71 631 605 2091 1525 Fragments arranged by size: 2091 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5147 5147 Size: 5152 BlpI GC'TnA_GC Cuts at: 2569 2569 Size: 5152 Bme1580I G_kGCm'C Cuts at: 1790 2211 2294 3213 4369 1790 Size: 421 83 919 1156 2573 Fragments arranged by size: 2573 1156 919 421 83 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5152 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2883 3841 442 Size: 416 53 83 399 24 1466 958 1753 Fragments arranged by size: 1753 1466 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5152 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2270 3812 1239 Size: 836 195 1542 2579 Fragments arranged by size: 2579 1542 836 195 Bpu10I CC'TnA_GC Cuts at: 2267 2267 Size: 5152 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3277 4142 4440 3277 Size: 865 298 3989 Fragments arranged by size: 3989 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2169 3815 882 Size: 617 670 1646 2219 Fragments arranged by size: 2219 1646 670 617 BsaAI yAC'GTr Cuts at: 493 1204 2943 493 Size: 711 1739 2702 Fragments arranged by size: 2702 1739 711 BsaBI GATnn'nnATC Cuts at: 5146 5146 Size: 5152 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2959 3341 3976 275 Size: 53 83 186 936 1426 382 635 1451 Fragments arranged by size: 1451 1426 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2363 3585 3983 4257 4404 4815 2363 Size: 1222 398 274 147 411 2700 Fragments arranged by size: 2700 1222 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4927 Fragments arranged by size: 4927 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5152 BseYI C'CCAG_C Cuts at: 4355 4851 4355 Size: 496 4656 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4579 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2602 3363 4391 1540 Size: 275 186 601 761 1028 2301 Fragments arranged by size: 2301 1028 761 601 275 186 BsiHKAI G_wGCw'C Cuts at: 2268 3213 3298 4369 2268 Size: 945 85 1071 3051 Fragments arranged by size: 3051 1071 945 85 BsmI GAATG_Cn' Cuts at: 2663 2756 2663 Size: 93 5059 BsmBI CGTCTCn'nnnn_ Cuts at: 2086 2086 Size: 5152 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2268 2294 2399 2471 3213 3298 Size: 399 421 57 26 105 72 742 85 Cuts at: 3298 4369 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 421 399 105 85 72 57 26 BspEI T'CCGG_A Cuts at: 4815 4815 Size: 5152 BspHI T'CATG_A Cuts at: 3041 4771 3041 Size: 1730 3422 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2507 844 Size: 1663 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3039 1394 Size: 429 1216 3507 Fragments arranged by size: 3507 1216 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3647 3821 65 Size: 1183 2399 174 1396 Fragments arranged by size: 2399 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3796 3983 1700 Size: 110 1986 187 2869 Fragments arranged by size: 2869 1986 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5152 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5152 BssSI C'ACGA_G Cuts at: 2059 3212 4224 2059 Size: 1153 1012 2987 Fragments arranged by size: 2987 1153 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5152 BstBI TT'CG_AA Cuts at: 3956 3956 Size: 5152 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5152 BstYI r'GATC_y Cuts at: 1972 2482 2849 3234 3251 4703 4789 4801 5147 Size: 510 367 385 17 1452 86 12 346 Cuts at: 5147 1972 Size: 1977 Fragments arranged by size: 1977 1452 510 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4600 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4413 Fragments arranged by size: 4413 683 56 BtsI GCAGTG_nn' Cuts at: 2047 2668 3462 3482 2047 Size: 621 794 20 3717 Fragments arranged by size: 3717 794 621 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5152 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5117 DraI TTT'AAA Cuts at: 2456 2456 Size: 5152 DraIII CAC_nnn'GTG Cuts at: 2300 2300 Size: 5152 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4159 809 Size: 507 2843 1802 Fragments arranged by size: 2843 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2599 3488 5117 Size: 54 1425 50 275 18 769 889 1629 Cuts at: 5117 8 Size: 43 Fragments arranged by size: 1629 1425 889 769 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2599 1537 Size: 275 787 4090 Fragments arranged by size: 4090 787 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3082 1353 Size: 1729 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3739 4113 4259 387 Size: 1064 138 2150 374 146 1280 Fragments arranged by size: 2150 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2266 2266 Size: 5152 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2270 3215 3812 4599 4867 1239 Size: 393 443 195 945 597 787 268 1524 Fragments arranged by size: 1524 945 787 597 443 393 268 195 EcoNI CCTnn'n_nnAGG Cuts at: 2129 2129 Size: 5152 EcoO109I rG'GnC_Cy Cuts at: 2096 2208 2096 Size: 112 5040 EcoRI G'AATT_C Cuts at: 2461 2839 2461 Size: 378 4774 FspI TGC'GCA Cuts at: 3658 3658 Size: 5152 HaeII r_GCGC'y Cuts at: 1678 2018 4299 1678 Size: 340 2281 2531 Fragments arranged by size: 2531 2281 340 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2064 2096 2268 2300 3793 Size: 32 135 32 492 32 172 32 1493 Cuts at: 3793 3825 3867 3899 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1493 492 172 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2496 2742 669 Size: 988 281 558 246 3079 Fragments arranged by size: 3079 988 558 281 246 HindIII A'AGCT_T Cuts at: 748 2144 748 Size: 1396 3756 HpaI GTT'AAC Cuts at: 2742 2742 Size: 5152 KpnI G_GTAC'C Cuts at: 2477 2477 Size: 5152 MfeI C'AATT_G Cuts at: 2751 2751 Size: 5152 MluI A'CGCG_T Cuts at: 5028 5028 Size: 5152 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2120 4266 4450 1305 Size: 815 2146 184 2007 Fragments arranged by size: 2146 2007 815 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3330 Fragments arranged by size: 3330 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3110 3469 3628 518 Size: 648 1944 359 159 2042 Fragments arranged by size: 2042 1944 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2037 2374 2568 3245 4393 4638 1606 Size: 431 337 194 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 431 337 245 194 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4600 NdeI CA'TA_TG Cuts at: 387 387 Size: 5152 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5152 NotI GC'GGCC_GC Cuts at: 2599 2599 Size: 5152 NspI r_CATG'y Cuts at: 1132 1229 2510 4055 1132 Size: 97 1281 1545 2229 Fragments arranged by size: 2229 1545 1281 97 PasI CC'CwG_GG Cuts at: 1348 2125 1348 Size: 777 4375 PciI A'CATG_T Cuts at: 4051 4051 Size: 5152 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5152 PmeI GTTT'AAAC Cuts at: 2456 2456 Size: 5152 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3221 3253 4734 4766 3221 Size: 32 1481 32 3607 Fragments arranged by size: 3607 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2096 2096 Size: 5152 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5152 PsiI TTA'TAA Cuts at: 2722 2722 Size: 5152 PspOMI G'GGCC_C Cuts at: 2207 2207 Size: 5152 PstI C_TGCA'G Cuts at: 830 2042 2504 830 Size: 1212 462 3478 Fragments arranged by size: 3478 1212 462 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5152 PvuII CAG'CTG Cuts at: 2374 2374 Size: 5152 SacI G_AGCT'C Cuts at: 2268 2268 Size: 5152 SalI G'TCGA_C Cuts at: 1936 2494 1936 Size: 558 4594 SanDI GG'GwC_CC Cuts at: 2096 2096 Size: 5152 SbfI CC_TGCA'GG Cuts at: 2504 2504 Size: 5152 ScaI AGT'ACT Cuts at: 3400 5112 3400 Size: 1712 3440 SfcI C'TryA_G Cuts at: 826 1045 2038 2500 3635 4316 4507 826 Size: 219 993 462 1135 681 191 1471 Fragments arranged by size: 1471 1135 993 681 462 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5152 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5152 SmaI CCC'GGG Cuts at: 1872 2479 1872 Size: 607 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3256 4157 4419 820 Size: 197 926 1313 901 262 1553 Fragments arranged by size: 1553 1313 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2943 493 Size: 2450 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5152 SphI G_CATG'C Cuts at: 1132 2510 1132 Size: 1378 3774 SspI AAT'ATT Cuts at: 5 52 3076 5 Size: 47 3024 2081 Fragments arranged by size: 3024 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5152 StyI C'CwwG_G Cuts at: 513 1065 2116 2591 513 Size: 552 1051 475 3074 Fragments arranged by size: 3074 1051 552 475 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2213 2235 3179 3196 3349 3534 3979 Size: 1255 633 22 944 17 153 185 445 Cuts at: 3979 325 Size: 1498 Fragments arranged by size: 1498 1255 944 633 445 185 153 22 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3398 5110 96 Size: 274 80 33 51 1443 1421 1712 138 Fragments arranged by size: 1712 1443 1421 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5112 5144 5112 Size: 32 5120 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5152 XbaI T'CTAG_A Cuts at: 2488 2488 Size: 5152 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5152 XmaI C'CCGG_G Cuts at: 1870 2477 1870 Size: 607 4545 XmnI GAAnn'nnTTC Cuts at: 3281 3281 Size: 5152 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2960 276 Size: 53 83 186 936 1426 2468 Fragments arranged by size: 2468 1426 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI