(Circular) (Six-base) MAPSORT of: pFN21AB7205.seq Check: 7903 from: 1 to: 5983 pFN21AB7205 5983 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3042 3042 Size: 5983 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3793 278 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3326 1937 Size: 1389 4594 Acc65I G'GTAC_C Cuts at: 3304 3304 Size: 5983 AclI AA'CG_TT Cuts at: 1563 4110 4483 1563 Size: 2547 373 3063 Fragments arranged by size: 3063 2547 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4046 5430 5698 1632 Size: 2414 1384 268 1917 Fragments arranged by size: 2414 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5786 AflIII A'CryG_T Cuts at: 1203 4882 5859 1203 Size: 3679 977 1327 Fragments arranged by size: 3679 1327 977 AgeI A'CCGG_T Cuts at: 4814 4814 Size: 5983 AhdI GACnn_n'nnGTC Cuts at: 4712 4712 Size: 5983 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5983 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5983 AlwNI CAG_nnn'CTG Cuts at: 2239 2830 3726 5298 2239 Size: 591 896 1572 2924 Fragments arranged by size: 2924 1572 896 591 ApaI G_GGCC'C Cuts at: 2083 2140 2747 2899 3278 2083 Size: 57 607 152 379 4788 Fragments arranged by size: 4788 607 379 152 57 ApaLI G'TGCA_C Cuts at: 2426 4040 5196 2426 Size: 1614 1156 3213 Fragments arranged by size: 3213 1614 1156 ApoI r'AATT_y Cuts at: 1493 3292 3518 3670 5779 1493 Size: 1799 226 152 2109 1697 Fragments arranged by size: 2109 1799 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4537 160 Size: 872 3505 1606 Fragments arranged by size: 3505 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 AvaI C'yCGr_G Cuts at: 1870 1943 2076 2378 2399 2751 2817 3056 3308 Size: 73 133 302 21 352 66 239 252 Cuts at: 3308 1870 Size: 4545 Fragments arranged by size: 4545 352 302 252 239 133 73 66 21 BamHI G'GATC_C Cuts at: 2784 3313 3680 2784 Size: 529 367 5087 Fragments arranged by size: 5087 529 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2434 3060 3178 3255 3304 Size: 325 217 627 647 626 118 77 49 Cuts at: 3304 4759 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 647 627 626 325 217 118 77 49 BanII G_rGCy'C Cuts at: 1391 2083 2140 2399 2737 2747 2899 3278 3302 Size: 692 57 259 338 10 152 379 24 Cuts at: 3302 1391 Size: 4072 Fragments arranged by size: 4072 692 379 338 259 152 57 24 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2654 928 Size: 971 755 4257 Fragments arranged by size: 4257 971 755 BbvCI CC'TCA_GC Cuts at: 2445 2459 2445 Size: 14 5969 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2407 2441 4174 4208 1903 Size: 34 470 34 1733 34 3678 Fragments arranged by size: 3678 1733 470 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3875 5085 3875 Size: 1210 4773 BclI T'GATC_A Cuts at: 1548 1548 Size: 5983 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2144 2516 4594 Size: 107 122 71 631 605 472 372 2078 Cuts at: 4594 136 Size: 1525 Fragments arranged by size: 2078 1525 631 605 472 372 122 107 71 BglII A'GATC_T Cuts at: 2712 5978 2712 Size: 3266 2717 BlpI GC'TnA_GC Cuts at: 3400 3400 Size: 5983 Bme1580I G_kGCm'C Cuts at: 1790 2083 2140 2353 2430 2439 2747 2899 3065 Size: 293 57 213 77 9 308 152 166 Cuts at: 3065 3183 3278 4044 5200 1790 Size: 118 95 766 1156 2573 Fragments arranged by size: 2573 1156 766 308 293 213 166 152 118 95 77 57 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5983 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3714 4672 442 Size: 416 53 83 399 24 2297 958 1753 Fragments arranged by size: 2297 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5983 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2197 2197 Size: 5983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2261 2363 2373 2431 2561 2591 2873 3156 Size: 1022 102 10 58 130 30 282 283 Cuts at: 3156 3265 4643 1239 Size: 109 1378 2579 Fragments arranged by size: 2579 1378 1022 283 282 130 109 102 58 30 10 Bpu10I CC'TnA_GC Cuts at: 2445 2459 2445 Size: 14 5969 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2828 4108 4973 5271 2828 Size: 1280 865 298 3540 Fragments arranged by size: 3540 1280 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3136 3241 4646 882 Size: 617 1637 105 1405 2219 Fragments arranged by size: 2219 1637 1405 617 105 BsaAI yAC'GTr Cuts at: 493 1204 2740 3774 493 Size: 711 1536 1034 2702 Fragments arranged by size: 2702 1536 1034 711 BsaBI GATnn'nnATC Cuts at: 5977 5977 Size: 5983 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3790 4172 4807 275 Size: 53 83 186 936 2257 382 635 1451 Fragments arranged by size: 2257 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2287 2955 4416 4814 5088 5235 5646 2287 Size: 668 1461 398 274 147 411 2624 Fragments arranged by size: 2624 1461 668 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2294 2324 2724 2754 2979 Size: 30 165 30 891 30 400 30 225 Cuts at: 2979 3009 1178 Size: 30 4152 Fragments arranged by size: 4152 891 400 225 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2275 2318 1186 Size: 1089 43 4851 Fragments arranged by size: 4851 1089 43 BseYI C'CCAG_C Cuts at: 2245 2769 3166 3229 5186 5682 2245 Size: 524 397 63 1957 496 2546 Fragments arranged by size: 2546 1957 524 496 397 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5410 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2134 3433 4194 5222 1540 Size: 275 186 133 1299 761 1028 2301 Fragments arranged by size: 2301 1299 1028 761 275 186 133 BsiHKAI G_wGCw'C Cuts at: 2430 4044 4129 5200 2430 Size: 1614 85 1071 3213 Fragments arranged by size: 3213 1614 1071 85 BsmI GAATG_Cn' Cuts at: 2800 3494 3587 2800 Size: 694 93 5196 Fragments arranged by size: 5196 694 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3007 3007 Size: 5983 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2140 2353 2399 2430 2439 2737 Size: 399 293 57 213 46 31 9 298 Cuts at: 2737 2747 2899 3065 3183 3278 3302 4044 4129 Size: 10 152 166 118 95 24 742 85 Cuts at: 4129 5200 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 298 293 213 166 152 118 95 85 57 46 31 24 10 9 BspEI T'CCGG_A Cuts at: 2287 2955 5646 2287 Size: 668 2691 2624 Fragments arranged by size: 2691 2624 668 BspHI T'CATG_A Cuts at: 2866 3872 5602 2866 Size: 1006 1730 3247 Fragments arranged by size: 3247 1730 1006 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3042 3338 844 Size: 2198 296 3489 Fragments arranged by size: 3489 2198 296 BsrBI CCG'CTC Cuts at: 1394 1823 3870 1394 Size: 429 2047 3507 Fragments arranged by size: 3507 2047 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3025 4478 4652 65 Size: 1183 1777 1453 174 1396 Fragments arranged by size: 1777 1453 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 2295 4627 4814 1700 Size: 110 199 286 2332 187 2869 Fragments arranged by size: 2869 2332 286 199 187 110 BsrGI T'GTAC_A Cuts at: 96 2310 96 Size: 2214 3769 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5983 BssSI C'ACGA_G Cuts at: 2196 2492 4043 5055 2196 Size: 296 1551 1012 3124 Fragments arranged by size: 3124 1551 1012 296 BstAPI GCAn_nnn'nTGC Cuts at: 794 2434 794 Size: 1640 4343 BstBI TT'CG_AA Cuts at: 4787 4787 Size: 5983 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5983 BstXI CCAn_nnnn'nTGG Cuts at: 2036 2036 Size: 5983 BstYI r'GATC_y Cuts at: 1972 2712 2784 3313 3680 4065 4082 5534 5620 Size: 740 72 529 367 385 17 1452 86 Cuts at: 5620 5632 5978 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 740 529 385 367 346 86 72 17 12 Bsu36I CC'TnA_GG Cuts at: 2258 2258 Size: 5983 BtgI C'CryG_G Cuts at: 513 1065 2004 2125 2157 2217 2439 2656 3124 Size: 552 939 121 32 60 222 217 468 Cuts at: 3124 3139 3279 513 Size: 15 140 3217 Fragments arranged by size: 3217 939 552 468 222 217 140 121 60 32 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5244 Fragments arranged by size: 5244 683 56 BtsI GCAGTG_nn' Cuts at: 2606 3499 4293 4313 2606 Size: 893 794 20 4276 Fragments arranged by size: 4276 893 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5983 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5948 DraI TTT'AAA Cuts at: 3287 3287 Size: 5983 DraIII CAC_nnn'GTG Cuts at: 2190 2434 2190 Size: 244 5739 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4990 809 Size: 507 3674 1802 Fragments arranged by size: 3674 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2192 3189 Size: 54 1425 50 275 18 177 185 997 Cuts at: 3189 3430 4319 5948 8 Size: 241 889 1629 43 Fragments arranged by size: 1629 1425 997 889 275 241 185 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3430 1537 Size: 275 1618 4090 Fragments arranged by size: 4090 1618 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3014 3913 1353 Size: 1661 899 3423 Fragments arranged by size: 3423 1661 899 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2111 2219 3016 4570 4944 5090 Size: 1064 138 522 108 797 1554 374 146 Cuts at: 5090 387 Size: 1280 Fragments arranged by size: 1554 1280 1064 797 522 374 146 138 108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 2363 2373 2431 2561 2591 2873 Size: 393 629 102 10 58 130 30 282 Cuts at: 2873 3156 3265 4046 4643 5430 5698 1239 Size: 283 109 781 597 787 268 1524 Fragments arranged by size: 1524 787 781 629 597 393 283 282 268 130 109 102 58 30 10 EcoO109I rG'GnC_Cy Cuts at: 2080 2093 2137 2536 2895 2896 3274 3275 2080 Size: 13 44 399 359 1 378 1 4788 Fragments arranged by size: 4788 399 378 359 44 13 1 1 EcoRI G'AATT_C Cuts at: 3292 3670 3292 Size: 378 5605 FseI GG_CCGG'CC Cuts at: 2013 2013 Size: 5983 FspI TGC'GCA Cuts at: 2474 2612 4489 2474 Size: 138 1877 3968 Fragments arranged by size: 3968 1877 138 HaeII r_GCGC'y Cuts at: 1678 2830 5130 1678 Size: 1152 2300 2531 Fragments arranged by size: 2531 2300 1152 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2979 3011 4624 4656 4698 Size: 32 135 32 1407 32 1613 32 42 Cuts at: 4698 4730 1373 Size: 32 2626 Fragments arranged by size: 2626 1613 1407 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3327 3573 669 Size: 988 281 1389 246 3079 Fragments arranged by size: 3079 1389 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5983 HpaI GTT'AAC Cuts at: 3573 3573 Size: 5983 KpnI G_GTAC'C Cuts at: 3308 3308 Size: 5983 MfeI C'AATT_G Cuts at: 3582 3582 Size: 5983 MluI A'CGCG_T Cuts at: 5859 5859 Size: 5983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2578 5097 5281 1305 Size: 1273 2519 184 2007 Fragments arranged by size: 2519 2007 1273 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2194 10 Size: 54 1425 343 362 3799 Fragments arranged by size: 3799 1425 362 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2639 3941 4300 4459 518 Size: 648 1473 1302 359 159 2042 Fragments arranged by size: 2042 1473 1302 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2143 2212 2239 2409 2478 2773 3097 3399 Size: 537 69 27 170 69 295 324 302 Cuts at: 3399 4076 5224 5469 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 537 324 302 295 245 170 69 69 27 NaeI GCC'GGC Cuts at: 2011 2011 Size: 5983 NcoI C'CATG_G Cuts at: 513 1065 2004 2125 2157 3124 3139 513 Size: 552 939 121 32 967 15 3357 Fragments arranged by size: 3357 967 939 552 121 32 15 NdeI CA'TA_TG Cuts at: 387 2929 387 Size: 2542 3441 NgoMIV G'CCGG_C Cuts at: 2009 2009 Size: 5983 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5983 NotI GC'GGCC_GC Cuts at: 3430 3430 Size: 5983 NspI r_CATG'y Cuts at: 1132 1229 3341 4886 1132 Size: 97 2112 1545 2229 Fragments arranged by size: 2229 2112 1545 97 PasI CC'CwG_GG Cuts at: 1348 2899 1348 Size: 1551 4432 PciI A'CATG_T Cuts at: 4882 4882 Size: 5983 PflMI CCAn_nnn'nTGG Cuts at: 1108 2190 2239 2830 1108 Size: 1082 49 591 4261 Fragments arranged by size: 4261 1082 591 49 PmeI GTTT'AAAC Cuts at: 3287 3287 Size: 5983 PmlI CAC'GTG Cuts at: 2740 2740 Size: 5983 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4052 4084 5565 5597 4052 Size: 32 1481 32 4438 Fragments arranged by size: 4438 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2093 2093 Size: 5983 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5983 PsiI TTA'TAA Cuts at: 3553 3553 Size: 5983 PspOMI G'GGCC_C Cuts at: 2079 2136 2743 2895 3274 2079 Size: 57 607 152 379 4788 Fragments arranged by size: 4788 607 379 152 57 PstI C_TGCA'G Cuts at: 830 3335 830 Size: 2505 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5983 PvuII CAG'CTG Cuts at: 2212 2239 2478 2773 3097 2212 Size: 27 239 295 324 5098 Fragments arranged by size: 5098 324 295 239 27 SalI G'TCGA_C Cuts at: 1936 3325 1936 Size: 1389 4594 SbfI CC_TGCA'GG Cuts at: 3335 3335 Size: 5983 ScaI AGT'ACT Cuts at: 4231 5943 4231 Size: 1712 4271 SfcI C'TryA_G Cuts at: 826 1045 3198 3331 4466 5147 5338 826 Size: 219 2153 133 1135 681 191 1471 Fragments arranged by size: 2153 1471 1135 681 219 191 133 SfiI GGCCn_nnn'nGGCC Cuts at: 2144 2516 2144 Size: 372 5611 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5983 SmaI CCC'GGG Cuts at: 1872 2078 2753 2819 3058 3310 1872 Size: 206 675 66 239 252 4545 Fragments arranged by size: 4545 675 252 239 206 66 SmlI C'TyrA_G Cuts at: 820 1017 1943 2843 4087 4988 5250 820 Size: 197 926 900 1244 901 262 1553 Fragments arranged by size: 1553 1244 926 901 900 262 197 SnaBI TAC'GTA Cuts at: 493 3774 493 Size: 3281 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5983 SphI G_CATG'C Cuts at: 1132 3341 1132 Size: 2209 3774 SspI AAT'ATT Cuts at: 5 52 3907 5 Size: 47 3855 2081 Fragments arranged by size: 3855 2081 47 StuI AGG'CCT Cuts at: 1517 2202 2276 1517 Size: 685 74 5224 Fragments arranged by size: 5224 685 74 StyI C'CwwG_G Cuts at: 513 1065 2004 2125 2157 3124 3139 3267 3422 Size: 552 939 121 32 967 15 128 155 Cuts at: 3422 513 Size: 3074 Fragments arranged by size: 3074 967 939 552 155 128 121 32 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4010 4027 4180 4365 4810 325 Size: 1255 2430 17 153 185 445 1498 Fragments arranged by size: 2430 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2310 2856 4229 Size: 274 80 33 51 1443 333 546 1373 Cuts at: 4229 5941 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1373 546 333 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2085 2117 2724 2756 5943 5975 2085 Size: 32 607 32 3187 32 2093 Fragments arranged by size: 3187 2093 607 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5983 XbaI T'CTAG_A Cuts at: 3319 3319 Size: 5983 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2673 2011 Size: 662 5321 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5983 XmaI C'CCGG_G Cuts at: 1870 2076 2751 2817 3056 3308 1870 Size: 206 675 66 239 252 4545 Fragments arranged by size: 4545 675 252 239 206 66 XmnI GAAnn'nnTTC Cuts at: 4112 4112 Size: 5983 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3791 276 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI