Restriction map for
pFN21AB7204
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# NO CUT ENZYMES ( 195 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII HphI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TspRI Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcsI 319 AAATTC [GA]^AATT[CT]
AluI 43 AGCT AG^CT
AlwNI 340 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 319 AAATTC [GA]^AATT[CT]
AspHI 37 GTGCTC G[AT]GC[AT]^C
BbvI 292 GCTGC GCAGC(8/12)
BlpI 39 GCTCAGC GC^T[ACGT]AGC
BmyI 37 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC
BsaI 304 GAGACC GGTCTC(1/5)
BsiHKAI 37 GTGCTC G[AT]GC[AT]^C
BsiYI 183 CCCCCAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 183 CCCCCAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 304 GAGAC GTCTC(1/5)
Bsp1286I 37 GTGCTC G[AGT]GC[ACT]^C
BspHI 512 TCATGA T^CATGA
BsrI 207 CCAGT ACTGG(1/-1)
BstYI 517 AGATCC [GA]^GATC[CT]
CelII 39 GCTCAGC GC^T[ACGT]AGC
DraII 106 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 26 TGGCCG [CT]^GGCC[GA]
EcoO109I 106 AGGGCCT [GA]G^G[ACGT]CC[CT]
Fnu4HI 292 GCTGC GC^[ACGT]GC
ItaI 292 GCTGC GC^[ACGT]GC
MaeIII 367 GTGAC ^GT[ACGT]AC
MflI 517 AGATCC [GA]^GATC[CT]
MseI 553 TTAA T^TAA
MwoI 226 GCCATCTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
RcaI 512 TCATGA T^CATGA
SmlI 221 CTCAAG C^T[CT][GA]AG
Tru9I 553 TTAA T^TAA
TseI 292 GCTGC G^C[AT]GC
Tsp45I 367 GTGAC ^GT[GC]AC
Tsp509I 320 AATT ^AATT
XcmI 53 CCATCTGTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 517 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AlwI 249 GATCC GGATC(4/5)
518 GATCC
BfaI 313 CTAG C^TAG
355 CTAG
BstF5I 417 CATCC GGATG(2/0)
300 GGATG
Cfr13I 3 GGCCC G^G[ACGT]CC
107 GGGCC
DdeI 40 CTCAG C^T[ACGT]AG
80 CTCAG
EarI 268 CTCTTC CTCTTC(1/4)
388 GAAGAG
FokI 417 CATCC GGATG(9/13)
300 GGATG
Ksp632I 268 CTCTTC CTCTTC(1/4)
388 GAAGAG
MaeI 313 CTAG C^TAG
355 CTAG
NlaIII 241 CATG CATG^
513 CATG
PleI 84 GACTC GAGTC(4/5)
412 GACTC
Sau96I 3 GGCCC G^G[ACGT]CC
107 GGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
BsaJI BssKI BstNI DpnII EcoRII
HaeIII HinfI MboI MboII MnlI
MvaI NdeII Sau3AI ScrFI TfiI