(Circular) (Six-base) MAPSORT of: pFN21AB7203.seq Check: 4388 from: 1 to: 6574 pFN21AB7203 6574 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4384 278 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3917 1937 Size: 1980 4594 Acc65I G'GTAC_C Cuts at: 3895 3895 Size: 6574 AclI AA'CG_TT Cuts at: 1563 4701 5074 1563 Size: 3138 373 3063 Fragments arranged by size: 3138 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2224 2716 3364 3644 4637 6021 6289 1632 Size: 592 492 648 280 993 1384 268 1917 Fragments arranged by size: 1917 1384 993 648 592 492 280 268 AfeI AGC'GCT Cuts at: 3668 3668 Size: 6574 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6377 AflIII A'CryG_T Cuts at: 1203 5473 6450 1203 Size: 4270 977 1327 Fragments arranged by size: 4270 1327 977 AgeI A'CCGG_T Cuts at: 2552 5405 2552 Size: 2853 3721 AhdI GACnn_n'nnGTC Cuts at: 5303 5303 Size: 6574 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6574 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2638 2834 1184 Size: 1454 196 4924 Fragments arranged by size: 4924 1454 196 AlwNI CAG_nnn'CTG Cuts at: 3511 4317 5889 3511 Size: 806 1572 4196 Fragments arranged by size: 4196 1572 806 ApaI G_GGCC'C Cuts at: 2665 2765 2853 2665 Size: 100 88 6386 Fragments arranged by size: 6386 100 88 ApaLI G'TGCA_C Cuts at: 2900 4631 5787 2900 Size: 1731 1156 3687 Fragments arranged by size: 3687 1731 1156 ApoI r'AATT_y Cuts at: 1493 3883 4109 4261 6370 1493 Size: 2390 226 152 2109 1697 Fragments arranged by size: 2390 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5128 160 Size: 872 4096 1606 Fragments arranged by size: 4096 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 AvaI C'yCGr_G Cuts at: 1870 1943 2061 2348 2852 3899 1870 Size: 73 118 287 504 1047 4545 Fragments arranged by size: 4545 1047 504 287 118 73 AvrII C'CTAG_G Cuts at: 2590 2590 Size: 6574 BamHI G'GATC_C Cuts at: 3904 4271 3904 Size: 367 6207 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3352 3514 3895 5350 618 Size: 325 217 627 1565 162 381 1455 1842 Fragments arranged by size: 1842 1565 1455 627 381 325 217 162 BanII G_rGCy'C Cuts at: 1391 2298 2526 2542 2665 2765 2772 2853 3077 Size: 907 228 16 123 100 7 81 224 Cuts at: 3077 3258 3893 1391 Size: 181 635 4072 Fragments arranged by size: 4072 907 635 228 224 181 123 100 81 16 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3449 928 Size: 971 1550 4053 Fragments arranged by size: 4053 1550 971 BbvCI CC'TCA_GC Cuts at: 2288 2448 2288 Size: 160 6414 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4765 4799 1903 Size: 34 2828 34 3678 Fragments arranged by size: 3678 2828 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4466 5676 4466 Size: 1210 5364 BclI T'GATC_A Cuts at: 1548 3600 1548 Size: 2052 4522 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2726 5185 136 Size: 107 122 71 631 605 1054 2459 1525 Fragments arranged by size: 2459 1525 1054 631 605 122 107 71 BglII A'GATC_T Cuts at: 6569 6569 Size: 6574 BlpI GC'TnA_GC Cuts at: 3991 3991 Size: 6574 Bme1580I G_kGCm'C Cuts at: 1790 2445 2665 2765 2853 2904 3201 3519 4635 Size: 655 220 100 88 51 297 318 1116 Cuts at: 4635 5791 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1116 655 318 297 220 100 88 51 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6574 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3471 3743 4305 Size: 416 53 83 399 24 2054 272 562 Cuts at: 4305 5263 442 Size: 958 1753 Fragments arranged by size: 2054 1753 958 562 416 399 272 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6574 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2952 2952 Size: 6574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2173 2222 2279 2476 2486 2567 2859 2884 Size: 934 49 57 197 10 81 292 25 Cuts at: 2884 2899 5234 1239 Size: 15 2335 2579 Fragments arranged by size: 2579 2335 934 292 197 81 57 49 25 15 10 Bpu10I CC'TnA_GC Cuts at: 2288 2384 2448 2288 Size: 96 64 6414 Fragments arranged by size: 6414 96 64 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3245 4699 5564 5862 3245 Size: 1454 865 298 3957 Fragments arranged by size: 3957 1454 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2543 5237 882 Size: 617 1044 2694 2219 Fragments arranged by size: 2694 2219 1044 617 BsaAI yAC'GTr Cuts at: 493 1204 2711 4365 493 Size: 711 1507 1654 2702 Fragments arranged by size: 2702 1654 1507 711 BsaBI GATnn'nnATC Cuts at: 6568 6568 Size: 6574 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2168 2326 2391 4381 Size: 53 83 186 936 635 158 65 1990 Cuts at: 4381 4763 5398 275 Size: 382 635 1451 Fragments arranged by size: 1990 1451 936 635 635 382 186 158 83 65 53 BsaWI w'CCGG_w Cuts at: 2552 2972 5007 5405 5679 5826 6237 2552 Size: 420 2035 398 274 147 411 2889 Fragments arranged by size: 2889 2035 420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2252 2282 2954 2984 3677 Size: 30 165 30 849 30 672 30 693 Cuts at: 3677 3707 3749 3779 1178 Size: 30 42 30 3973 Fragments arranged by size: 3973 849 693 672 165 42 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2881 3227 3773 1186 Size: 1695 346 546 3987 Fragments arranged by size: 3987 1695 546 346 BseYI C'CCAG_C Cuts at: 2658 2941 3200 5777 6273 2658 Size: 283 259 2577 496 2959 Fragments arranged by size: 2959 2577 496 283 259 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3452 3524 1163 Size: 573 1716 72 4213 Fragments arranged by size: 4213 1716 573 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4024 4785 5813 1540 Size: 275 186 2023 761 1028 2301 Fragments arranged by size: 2301 2023 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2298 2526 2772 2904 3085 3231 3258 3658 4635 Size: 228 246 132 181 146 27 400 977 Cuts at: 4635 4720 5791 2298 Size: 85 1071 3081 Fragments arranged by size: 3081 1071 977 400 246 228 181 146 132 85 27 BsmI GAATG_Cn' Cuts at: 4085 4178 4085 Size: 93 6481 BsmBI CGTCTCn'nnnn_ Cuts at: 2536 2536 Size: 6574 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2298 2445 2526 2542 2665 2765 2772 Size: 399 508 147 81 16 123 100 7 Cuts at: 2772 2853 2904 3077 3085 3201 3231 3258 3519 Size: 81 51 173 8 116 30 27 261 Cuts at: 3519 3658 3893 4635 4720 5791 1391 Size: 139 235 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 508 399 261 235 173 147 139 123 116 100 85 81 81 51 30 27 16 8 7 BspEI T'CCGG_A Cuts at: 2972 6237 2972 Size: 3265 3309 BspHI T'CATG_A Cuts at: 4463 6193 4463 Size: 1730 4844 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2424 3929 844 Size: 1580 1505 3489 Fragments arranged by size: 3489 1580 1505 BsrBI CCG'CTC Cuts at: 1394 1823 2206 4461 1394 Size: 429 383 2255 3507 Fragments arranged by size: 3507 2255 429 383 BsrDI GCAATG_nn' Cuts at: 65 1248 3618 5069 5243 65 Size: 1183 2370 1451 174 1396 Fragments arranged by size: 2370 1451 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2552 5218 5405 1700 Size: 110 742 2666 187 2869 Fragments arranged by size: 2869 2666 742 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6574 BssHII G'CGCG_C Cuts at: 1925 2456 1925 Size: 531 6043 BssSI C'ACGA_G Cuts at: 2926 4634 5646 2926 Size: 1708 1012 3854 Fragments arranged by size: 3854 1708 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3415 794 Size: 2621 3953 BstBI TT'CG_AA Cuts at: 5378 5378 Size: 6574 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6574 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3487 3556 2011 Size: 1476 69 5029 Fragments arranged by size: 5029 1476 69 BstYI r'GATC_y Cuts at: 1972 3904 4271 4656 4673 6125 6211 6223 6569 Size: 1932 367 385 17 1452 86 12 346 Cuts at: 6569 1972 Size: 1977 Fragments arranged by size: 1977 1932 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6022 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5835 Fragments arranged by size: 5835 683 56 BtsI GCAGTG_nn' Cuts at: 2816 2961 3536 3658 4090 4884 4904 2816 Size: 145 575 122 432 794 20 4486 Fragments arranged by size: 4486 794 575 432 145 122 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6574 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6539 DraI TTT'AAA Cuts at: 3878 3878 Size: 6574 DraIII CAC_nnn'GTG Cuts at: 3334 3334 Size: 6574 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5581 809 Size: 507 4265 1802 Fragments arranged by size: 4265 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 2361 2569 Size: 54 1425 50 275 18 381 150 208 Cuts at: 2569 2718 2784 3141 3180 3609 4021 4910 6539 Size: 149 66 357 39 429 412 889 1629 Cuts at: 6539 8 Size: 43 Fragments arranged by size: 1629 1425 889 429 412 381 357 275 208 150 149 66 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 4021 1537 Size: 275 2209 4090 Fragments arranged by size: 4090 2209 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3663 4504 1353 Size: 2310 841 3423 Fragments arranged by size: 3423 2310 841 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3120 5161 5535 5681 387 Size: 1064 138 1531 2041 374 146 1280 Fragments arranged by size: 2041 1531 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2296 2524 2770 3256 2296 Size: 228 246 486 5614 Fragments arranged by size: 5614 486 246 228 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2173 2222 2224 2279 2476 2486 2567 Size: 393 541 49 2 55 197 10 81 Cuts at: 2567 2716 2859 2884 2899 3364 3644 4637 5234 Size: 149 143 25 15 465 280 993 597 Cuts at: 5234 6021 6289 1239 Size: 787 268 1524 Fragments arranged by size: 1524 993 787 597 541 465 393 280 268 197 149 143 81 55 49 25 15 10 2 EcoO109I rG'GnC_Cy Cuts at: 2244 2317 2587 2662 2761 2796 2883 3275 3508 Size: 73 270 75 99 35 87 392 233 Cuts at: 3508 2244 Size: 5310 Fragments arranged by size: 5310 392 270 233 99 87 75 73 35 EcoRI G'AATT_C Cuts at: 3883 4261 3883 Size: 378 6196 FspI TGC'GCA Cuts at: 5080 5080 Size: 6574 HaeII r_GCGC'y Cuts at: 1678 2384 3670 5721 1678 Size: 706 1286 2051 2531 Fragments arranged by size: 2531 2051 1286 706 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2967 2999 3441 3473 3749 Size: 32 135 32 1395 32 442 32 276 Cuts at: 3749 3781 5215 5247 5289 5321 1373 Size: 32 1434 32 42 32 2626 Fragments arranged by size: 2626 1434 1395 442 276 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3721 3918 4164 669 Size: 988 281 1783 197 246 3079 Fragments arranged by size: 3079 1783 988 281 246 197 HindIII A'AGCT_T Cuts at: 748 748 Size: 6574 HpaI GTT'AAC Cuts at: 4164 4164 Size: 6574 KpnI G_GTAC'C Cuts at: 3899 3899 Size: 6574 MfeI C'AATT_G Cuts at: 4173 4173 Size: 6574 MluI A'CGCG_T Cuts at: 6450 6450 Size: 6574 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5688 5872 1305 Size: 4383 184 2007 Fragments arranged by size: 4383 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2786 3143 3182 3611 10 Size: 54 1425 343 954 357 39 429 2973 Fragments arranged by size: 2973 1425 954 429 357 343 54 39 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4532 4891 5050 518 Size: 648 843 2523 359 159 2042 Fragments arranged by size: 2523 2042 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2088 2266 2447 2503 3173 3990 4667 5815 Size: 482 178 181 56 670 817 677 1148 Cuts at: 5815 6060 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 817 677 670 482 245 181 178 56 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6022 NdeI CA'TA_TG Cuts at: 387 387 Size: 6574 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6574 NotI GC'GGCC_GC Cuts at: 4021 4021 Size: 6574 NspI r_CATG'y Cuts at: 1132 1229 2026 2619 3152 3418 3932 5477 1132 Size: 97 797 593 533 266 514 1545 2229 Fragments arranged by size: 2229 1545 797 593 533 514 266 97 PasI CC'CwG_GG Cuts at: 1348 2248 2373 2610 2846 3247 1348 Size: 900 125 237 236 401 4675 Fragments arranged by size: 4675 900 401 237 236 125 PciI A'CATG_T Cuts at: 5473 5473 Size: 6574 PflMI CCAn_nnn'nTGG Cuts at: 1108 2104 1108 Size: 996 5578 PfoI T'CCnGG_A Cuts at: 2463 2776 2463 Size: 313 6261 PmeI GTTT'AAAC Cuts at: 3878 3878 Size: 6574 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4643 4675 6156 6188 4643 Size: 32 1481 32 5029 Fragments arranged by size: 5029 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2587 2796 3275 2587 Size: 209 479 5886 Fragments arranged by size: 5886 479 209 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6574 PsiI TTA'TAA Cuts at: 4144 4144 Size: 6574 PspOMI G'GGCC_C Cuts at: 2661 2761 2849 2661 Size: 100 88 6386 Fragments arranged by size: 6386 100 88 PstI C_TGCA'G Cuts at: 830 2093 2421 2956 3653 3926 830 Size: 1263 328 535 697 273 3478 Fragments arranged by size: 3478 1263 697 535 328 273 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6574 PvuII CAG'CTG Cuts at: 2088 2266 2503 2088 Size: 178 237 6159 Fragments arranged by size: 6159 237 178 SacI G_AGCT'C Cuts at: 2298 2526 2772 3258 2298 Size: 228 246 486 5614 Fragments arranged by size: 5614 486 246 228 SalI G'TCGA_C Cuts at: 1936 3916 1936 Size: 1980 4594 SapI GCTCTTCn'nnn_ Cuts at: 3663 3663 Size: 6574 SbfI CC_TGCA'GG Cuts at: 2421 3926 2421 Size: 1505 5069 ScaI AGT'ACT Cuts at: 3866 4822 6534 3866 Size: 956 1712 3906 Fragments arranged by size: 3906 1712 956 SexAI A'CCwGG_T Cuts at: 2637 2637 Size: 6574 SfcI C'TryA_G Cuts at: 826 1045 2089 2417 2597 2952 3649 3739 3922 Size: 219 1044 328 180 355 697 90 183 Cuts at: 3922 5057 5738 5929 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1044 697 681 355 328 219 191 183 180 90 SfiI GGCCn_nnn'nGGCC Cuts at: 2726 2726 Size: 6574 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6574 SmaI CCC'GGG Cuts at: 1872 2063 2350 3901 1872 Size: 191 287 1551 4545 Fragments arranged by size: 4545 1551 287 191 SmlI C'TyrA_G Cuts at: 820 1017 1943 3224 4678 5579 5841 820 Size: 197 926 1281 1454 901 262 1553 Fragments arranged by size: 1553 1454 1281 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4365 493 Size: 3872 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6574 SphI G_CATG'C Cuts at: 1132 2026 3418 3932 1132 Size: 894 1392 514 3774 Fragments arranged by size: 3774 1392 894 514 SspI AAT'ATT Cuts at: 5 52 4498 5 Size: 47 4446 2081 Fragments arranged by size: 4446 2081 47 StuI AGG'CCT Cuts at: 1517 2237 3830 1517 Size: 720 1593 4261 Fragments arranged by size: 4261 1593 720 StyI C'CwwG_G Cuts at: 513 1065 2590 3339 4013 513 Size: 552 1525 749 674 3074 Fragments arranged by size: 3074 1525 749 674 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2096 2795 4601 4618 4771 4956 5401 Size: 1255 516 699 1806 17 153 185 445 Cuts at: 5401 325 Size: 1498 Fragments arranged by size: 1806 1498 1255 699 516 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3864 4820 6532 Size: 274 80 33 51 1443 1887 956 1712 Cuts at: 6532 96 Size: 138 Fragments arranged by size: 1887 1712 1443 956 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2252 2284 2954 2986 3180 3212 3320 3352 6534 Size: 32 670 32 194 32 108 32 3182 Cuts at: 6534 6566 2252 Size: 32 2260 Fragments arranged by size: 3182 2260 670 194 108 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6574 XbaI T'CTAG_A Cuts at: 3910 3910 Size: 6574 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2197 3001 3084 3553 3556 2197 Size: 804 83 469 3 5215 Fragments arranged by size: 5215 804 469 83 3 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6574 XmaI C'CCGG_G Cuts at: 1870 2061 2348 3899 1870 Size: 191 287 1551 4545 Fragments arranged by size: 4545 1551 287 191 XmnI GAAnn'nnTTC Cuts at: 4703 4703 Size: 6574 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4382 276 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfoI SrfI SwaI