Restriction map for
pFN21AB7190
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BbeI BbrPI BbvCI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI Hin1I
HindIII HinfI HpaI KasI KpnI
Ksp632I MaeI MaeII MamI MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I TspRI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AlwI 153 GATCC GGATC(4/5)
AocI 364 CCTAAGG CC^T[ACGT]AGG
Aor51HI 85 AGCGCT AGC^GCT
AspHI 258 GAGCTC G[AT]GC[AT]^C
BalI 62 TGGCCA TGG^CCA
BanII 258 GAGCTC G[GA]GC[CT]^C
BbsI 316 GAAGAC GAAGAC(2/6)
BciVI 389 GTATCC GTATCC(6/5)
BcnI 5 CCCGG CC^[GC]GG
BglI 64 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 258 GAGCTC G[AGT]GC[ACT]^C
BpmI 89 CTCCAG CTGGAG(16/14)
BpuAI 316 GAAGAC GAAGAC(2/6)
BsaI 307 GGTCTC GGTCTC(1/5)
BsiHKAI 258 GAGCTC G[AT]GC[AT]^C
BsmAI 308 GTCTC GTCTC(1/5)
Bsp1286I 258 GAGCTC G[AGT]GC[ACT]^C
BsrFI 34 ACCGGC [GA]^CCGG[CT]
BsrI 97 ACTGG ACTGG(1/-1)
BssHII 76 GCGCGC G^CGCGC
BstYI 152 AGATCC [GA]^GATC[CT]
Bsu36I 364 CCTAAGG CC^T[ACGT]AGG
Cfr10I 34 ACCGGC [GA]^CCGG[CT]
Cfr13I 3 GGCCC G^G[ACGT]CC
DpnII 153 GATC ^GATC
EaeI 62 TGGCCA [CT]^GGCC[GA]
Eco47III 85 AGCGCT AGC^GCT
Eco81I 364 CCTAAGG CC^T[ACGT]AGG
EcoT14I 68 CCAAGG C^C[AT][AT]GG
HgaI 384 GCGTC GACGC(5/10)
HincII 171 GTCGAC GT[CT]^[GA]AC
HindII 171 GTCGAC GT[CT]^[GA]AC
HphI 267 GGTGA GGTGA(8/7)
KspI 49 CCGCGG CCGC^GG
MaeIII 352 GTCAC ^GT[ACGT]AC
MboI 153 GATC ^GATC
MflI 152 AGATCC [GA]^GATC[CT]
MluNI 62 TGGCCA TGG^CCA
MscI 62 TGGCCA TGG^CCA
MslI 354 CACTATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 5 CCCGG CC^[GC]GG
NdeII 153 GATC ^GATC
PflMI 91 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 58 CAGCTG CAG^CTG
SacI 258 GAGCTC GAGCT^C
SacII 49 CCGCGG CCGC^GG
SalI 171 GTCGAC G^TCGAC
Sau3AI 153 GATC ^GATC
Sau96I 3 GGCCC G^G[ACGT]CC
SfcI 123 CTACAG C^T[GA][CT]AG
StuI 293 AGGCCT AGG^CCT
StyI 68 CCAAGG C^C[AT][AT]GG
TaqI 172 TCGA T^CGA
Tsp45I 352 GTCAC ^GT[GC]AC
TthHB8I 172 TCGA T^CGA
Van91I 91 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 152 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 30 GTCTAC GT^[AC][GT]AC
171 GTCGAC
AlwNI 92 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
205 CAGCGCCTG
BanI 45 GGCACC G^G[CT][GA]CC
133 GGCACC
BcgI 173 CGACTGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
15 GCAGACAGCTCG
BsiYI 91 CCAGCGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
255 CCAGAGCTCGG
BslI 91 CCAGCGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
255 CCAGAGCTCGG
BstF5I 186 CATCC GGATG(2/0)
372 CATCC
BstNI 130 CCAGG CC^[AT]GG
210 CCTGG
DdeI 176 CTGAG C^T[ACGT]AG
365 CTAAG
EcoRII 130 CCAGG ^CC[AT]GG
210 CCTGG
FokI 186 CATCC GGATG(9/13)
372 CATCC
HapII 6 CCGG C^CGG
35 CCGG
HpaII 6 CCGG C^CGG
35 CCGG
MboII 108 GAAGA GAAGA(8/7)
316 GAAGA
MspA1I 49 CCGCGG C[AC]G^C[GT]G
58 CAGCTG
MspI 6 CCGG C^CGG
35 CCGG
MvaI 130 CCAGG CC^[AT]GG
210 CCTGG
NlaIV 45 GGCACC GG[ACGT]^[ACGT]CC
133 GGCACC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AccII AciI AluI BbvI BsaJI
BssKI BstUI Cac8I CfoI DsaI
Fnu4HI HaeII HaeIII HhaI HinP1I
ItaI MnlI MvnI MwoI ScrFI
TseI