Restriction map for
pFN21AB7188
(Linear) (Six-base) MAPSORT of: KIBB7188.seq Check: 8251 from: 1 to: 1386
KIBB7188.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 818 1386
Size: 818 568
AlwNI CAG_nnn'CTG
Cuts at: 0 1258 1386
Size: 1258 128
ApaLI G'TGCA_C
Cuts at: 0 613 994 1386
Size: 613 381 392
Fragments arranged by size:
613 392 381
AvaI C'yCGr_G
Cuts at: 0 57 597 658 853 864 870 902 930
Size: 57 540 61 195 11 6 32 28
Cuts at: 930 1386
Size: 456
Fragments arranged by size:
540 456 195 61 57 32 28 11
6
AvrII C'CTAG_G
Cuts at: 0 165 1386
Size: 165 1221
BanI G'GyrC_C
Cuts at: 0 101 169 631 739 822 1027 1054 1170
Size: 101 68 462 108 83 205 27 116
Cuts at: 1170 1327 1386
Size: 157 59
Fragments arranged by size:
462 205 157 116 108 101 83 68
59 27
BanII G_rGCy'C
Cuts at: 0 604 658 854 871 1386
Size: 604 54 196 17 515
Fragments arranged by size:
604 515 196 54 17
BbeI G_GCGC'C
Cuts at: 0 173 743 826 1031 1174 1386
Size: 173 570 83 205 143 212
Fragments arranged by size:
570 212 205 173 143 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 166 744 1386
Size: 166 578 642
Fragments arranged by size:
642 578 166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3 37 69 103 548 582 1386
Size: 3 34 32 34 445 34 804
Fragments arranged by size:
804 445 34 34 34 32 3
BglI GCCn_nnn'nGGC
Cuts at: 0 490 1386
Size: 490 896
BglII A'GATC_T
Cuts at: 0 194 1386
Size: 194 1192
Bme1580I G_kGCm'C
Cuts at: 0 617 998 1057 1386
Size: 617 381 59 329
Fragments arranged by size:
617 381 329 59
BmgBI CAC'GTC
Cuts at: 0 449 1386
Size: 449 937
BmrI ACTGGGnnnn_n'
Cuts at: 0 522 904 1386
Size: 522 382 482
Fragments arranged by size:
522 482 382
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 879 1386
Size: 879 507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 640 1235 1386
Size: 183 457 595 151
Fragments arranged by size:
595 457 183 151
Bpu10I CC'TnA_GC
Cuts at: 0 652 1009 1386
Size: 652 357 377
Fragments arranged by size:
652 377 357
BsaAI yAC'GTr
Cuts at: 0 759 948 1386
Size: 759 189 438
Fragments arranged by size:
759 438 189
BsaHI Gr'CG_yC
Cuts at: 0 170 740 823 859 1028 1171 1386
Size: 170 570 83 36 169 143 215
Fragments arranged by size:
570 215 170 169 143 83 36
BsaWI w'CCGG_w
Cuts at: 0 274 1386
Size: 274 1112
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 30 111 421 1030 1117 1386
Size: 30 81 310 609 87 269
Fragments arranged by size:
609 310 269 87 81 30
BseYI C'CCAG_C
Cuts at: 0 380 1112 1362 1386
Size: 380 732 250 24
Fragments arranged by size:
732 380 250 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1148 1339 1386
Size: 400 748 191 47
Fragments arranged by size:
748 400 191 47
BsiEI CG_ry'CG
Cuts at: 0 462 1386
Size: 462 924
BsiHKAI G_wGCw'C
Cuts at: 0 617 998 1109 1386
Size: 617 381 111 277
Fragments arranged by size:
617 381 277 111
BsmBI CGTCTCn'nnnn_
Cuts at: 0 42 760 1386
Size: 42 718 626
Fragments arranged by size:
718 626 42
Bsp1286I G_dGCh'C
Cuts at: 0 604 617 658 854 871 998 1057 1109
Size: 604 13 41 196 17 127 59 52
Cuts at: 1109 1386
Size: 277
Fragments arranged by size:
604 277 196 127 59 52 41 17
13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 818 1386
Size: 818 568
BsrBI CCG'CTC
Cuts at: 0 280 1041 1249 1386
Size: 280 761 208 137
Fragments arranged by size:
761 280 208 137
BsrFI r'CCGG_y
Cuts at: 0 502 692 974 1386
Size: 502 190 282 412
Fragments arranged by size:
502 412 282 190
BssHII G'CGCG_C
Cuts at: 0 1177 1386
Size: 1177 209
BssSI C'ACGA_G
Cuts at: 0 1083 1386
Size: 1083 303
BstEII G'GTnAC_C
Cuts at: 0 369 1386
Size: 369 1017
BstYI r'GATC_y
Cuts at: 0 194 1386
Size: 194 1192
BtgI C'CryG_G
Cuts at: 0 608 1022 1188 1215 1286 1386
Size: 608 414 166 27 71 100
Fragments arranged by size:
608 414 166 100 71 27
BtsI GCAGTG_nn'
Cuts at: 0 440 543 1386
Size: 440 103 843
Fragments arranged by size:
843 440 103
DraIII CAC_nnn'GTG
Cuts at: 0 1002 1386
Size: 1002 384
EaeI y'GGCC_r
Cuts at: 0 152 326 349 393 575 605 839 953
Size: 152 174 23 44 182 30 234 114
Cuts at: 953 1386
Size: 433
Fragments arranged by size:
433 234 182 174 152 114 44 30
23
EarI CTCTTCn'nnn_
Cuts at: 0 88 1386
Size: 88 1298
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 694 1129 1386
Size: 694 435 257
Fragments arranged by size:
694 435 257
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 640 1235 1386
Size: 183 457 595 151
Fragments arranged by size:
595 457 183 151
EcoNI CCTnn'n_nnAGG
Cuts at: 0 622 1012 1386
Size: 622 390 374
Fragments arranged by size:
622 390 374
EcoO109I rG'GnC_Cy
Cuts at: 0 648 898 1350 1386
Size: 648 250 452 36
Fragments arranged by size:
648 452 250 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 768 1386
Size: 768 618
HaeII r_GCGC'y
Cuts at: 0 173 743 789 826 910 1031 1174 1386
Size: 173 570 46 37 84 121 143 212
Fragments arranged by size:
570 212 173 143 121 84 46 37
KasI G'GCGC_C
Cuts at: 0 169 739 822 1027 1170 1386
Size: 169 570 83 205 143 216
Fragments arranged by size:
570 216 205 169 143 83
MscI TGG'CCA
Cuts at: 0 328 351 395 1386
Size: 328 23 44 991
Fragments arranged by size:
991 328 44 23
MslI CAynn'nnrTG
Cuts at: 0 334 1386
Size: 334 1052
MspA1I CmG'CkG
Cuts at: 0 176 991 1024 1112 1190 1202 1217 1288
Size: 176 815 33 88 78 12 15 71
Cuts at: 1288 1311 1362 1386
Size: 23 51 24
Fragments arranged by size:
815 176 88 78 71 51 33 24
23 15 12
NaeI GCC'GGC
Cuts at: 0 504 694 976 1386
Size: 504 190 282 410
Fragments arranged by size:
504 410 282 190
NarI GG'CG_CC
Cuts at: 0 170 740 823 1028 1171 1386
Size: 170 570 83 205 143 215
Fragments arranged by size:
570 215 205 170 143 83
NgoMIV G'CCGG_C
Cuts at: 0 502 692 974 1386
Size: 502 190 282 412
Fragments arranged by size:
502 412 282 190
NspI r_CATG'y
Cuts at: 0 480 1386
Size: 480 906
PasI CC'CwG_GG
Cuts at: 0 1050 1386
Size: 1050 336
PfoI T'CCnGG_A
Cuts at: 0 722 1337 1386
Size: 722 615 49
Fragments arranged by size:
722 615 49
PpuMI rG'GwC_Cy
Cuts at: 0 648 1386
Size: 648 738
PvuII CAG'CTG
Cuts at: 0 1112 1311 1386
Size: 1112 199 75
Fragments arranged by size:
1112 199 75
RsrII CG'GwC_CG
Cuts at: 0 73 1185 1386
Size: 73 1112 201
Fragments arranged by size:
1112 201 73
SacII CC_GC'GG
Cuts at: 0 1025 1191 1218 1289 1386
Size: 1025 166 27 71 97
Fragments arranged by size:
1025 166 97 71 27
SfcI C'TryA_G
Cuts at: 0 702 1386
Size: 702 684
SfoI GGC'GCC
Cuts at: 0 171 741 824 1029 1172 1386
Size: 171 570 83 205 143 214
Fragments arranged by size:
570 214 205 171 143 83
SmaI CCC'GGG
Cuts at: 0 599 660 932 1386
Size: 599 61 272 454
Fragments arranged by size:
599 454 272 61
SphI G_CATG'C
Cuts at: 0 480 1386
Size: 480 906
SrfI GCCC'GGGC
Cuts at: 0 932 1386
Size: 932 454
StyI C'CwwG_G
Cuts at: 0 165 530 1386
Size: 165 365 856
Fragments arranged by size:
856 365 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 388 504 1386
Size: 388 116 882
Fragments arranged by size:
882 388 116
TatI w'GTAC_w
Cuts at: 0 518 1386
Size: 518 868
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 615 1386
Size: 615 771
XmaI C'CCGG_G
Cuts at: 0 597 658 930 1386
Size: 597 61 272 456
Fragments arranged by size:
597 456 272 61
XmnI GAAnn'nnTTC
Cuts at: 0 751 1386
Size: 751 635
Enzymes that do cut:
AarI AlwNI ApaLI AvaI AvrII BanI BanII BbeI
BbsI BcgI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII
BssSI BstEII BstYI BtgI BtsI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I FalI HaeII KasI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PfoI
PpuMI PvuII RsrII SacII SfcI SfoI SmaI SphI
SrfI StyI TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApoI AscI
AseI AsiSI BaeI BamHI BbvCI BciVI BclI BfrBI
BlpI BmtI BpuEI BsaI BsaBI BsaXI BsiWI BsmI
BspEI BspHI BsrDI BsrGI BstAPI BstBI BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
SacI SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SspI StuI SwaI
TstI Tth111I XbaI XhoI ZraI