(Circular) (Six-base) MAPSORT of: pFN21AB7185.seq Check: 7574 from: 1 to: 6037 pFN21AB7185 6037 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2330 2330 Size: 6037 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3847 278 Size: 53 83 186 936 2311 2468 Fragments arranged by size: 2468 2311 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2761 3380 1937 Size: 824 619 4594 Fragments arranged by size: 4594 824 619 Acc65I G'GTAC_C Cuts at: 2127 3358 2127 Size: 1231 4806 AclI AA'CG_TT Cuts at: 1563 4164 4537 1563 Size: 2601 373 3063 Fragments arranged by size: 3063 2601 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3033 4100 5484 5752 1632 Size: 1401 1067 1384 268 1917 Fragments arranged by size: 1917 1401 1384 1067 268 AfeI AGC'GCT Cuts at: 2642 2866 2642 Size: 224 5813 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5840 AflIII A'CryG_T Cuts at: 1203 4936 5913 1203 Size: 3733 977 1327 Fragments arranged by size: 3733 1327 977 AgeI A'CCGG_T Cuts at: 4868 4868 Size: 6037 AhdI GACnn_n'nnGTC Cuts at: 4766 4766 Size: 6037 AleI CACnn'nnGTG Cuts at: 1166 2710 1166 Size: 1544 4493 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6037 AlwNI CAG_nnn'CTG Cuts at: 3780 5352 3780 Size: 1572 4465 ApaI G_GGCC'C Cuts at: 2512 2512 Size: 6037 ApaLI G'TGCA_C Cuts at: 4094 5250 4094 Size: 1156 4881 ApoI r'AATT_y Cuts at: 1493 2117 2655 3113 3125 3346 3572 3724 5833 Size: 624 538 458 12 221 226 152 2109 Cuts at: 5833 1493 Size: 1697 Fragments arranged by size: 2109 1697 624 538 458 226 221 152 12 AseI AT'TA_AT Cuts at: 160 1032 4591 160 Size: 872 3559 1606 Fragments arranged by size: 3559 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 AvaI C'yCGr_G Cuts at: 1870 1943 2829 3362 1870 Size: 73 886 533 4545 Fragments arranged by size: 4545 886 533 73 BamHI G'GATC_C Cuts at: 3367 3734 3367 Size: 367 5670 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2012 2127 2704 3358 4813 Size: 325 217 627 225 115 577 654 1455 Cuts at: 4813 618 Size: 1842 Fragments arranged by size: 1842 1455 654 627 577 325 225 217 115 BanII G_rGCy'C Cuts at: 1391 2085 2512 3356 1391 Size: 694 427 844 4072 Fragments arranged by size: 4072 844 694 427 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3021 928 Size: 971 1122 3944 Fragments arranged by size: 3944 1122 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2726 2760 4228 4262 1903 Size: 34 789 34 1468 34 3678 Fragments arranged by size: 3678 1468 789 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3929 5139 3929 Size: 1210 4827 BclI T'GATC_A Cuts at: 1548 1548 Size: 6037 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2833 4648 136 Size: 107 122 71 631 605 1161 1815 1525 Fragments arranged by size: 1815 1525 1161 631 605 122 107 71 BglII A'GATC_T Cuts at: 6032 6032 Size: 6037 BlpI GC'TnA_GC Cuts at: 2602 3454 2602 Size: 852 5185 Bme1580I G_kGCm'C Cuts at: 1790 2512 4098 5254 1790 Size: 722 1586 1156 2573 Fragments arranged by size: 2573 1586 1156 722 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6037 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3343 3768 4726 Size: 416 53 83 399 24 1926 425 958 Cuts at: 4726 442 Size: 1753 Fragments arranged by size: 1926 1753 958 425 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6037 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4697 1239 Size: 3458 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2061 4162 5027 5325 2061 Size: 2101 865 298 2773 Fragments arranged by size: 2773 2101 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2351 4700 882 Size: 617 852 2349 2219 Fragments arranged by size: 2349 2219 852 617 BsaAI yAC'GTr Cuts at: 493 1204 3828 493 Size: 711 2624 2702 Fragments arranged by size: 2702 2624 711 BsaBI GATnn'nnATC Cuts at: 6031 6031 Size: 6037 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3844 4226 4861 275 Size: 53 83 186 936 2311 382 635 1451 Fragments arranged by size: 2311 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4470 4868 5142 5289 5700 4470 Size: 398 274 147 411 4807 Fragments arranged by size: 4807 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5812 Fragments arranged by size: 5812 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6037 BseYI C'CCAG_C Cuts at: 2643 3301 5240 5736 2643 Size: 658 1939 496 2944 Fragments arranged by size: 2944 1939 658 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2132 2602 1163 Size: 573 396 470 4598 Fragments arranged by size: 4598 573 470 396 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3487 4248 5276 1540 Size: 275 186 1486 761 1028 2301 Fragments arranged by size: 2301 1486 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2310 2498 3282 4098 4183 5254 2310 Size: 188 784 816 85 1071 3093 Fragments arranged by size: 3093 1071 816 784 188 85 BsmI GAATG_Cn' Cuts at: 3548 3641 3548 Size: 93 5944 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2310 2498 2512 3282 3356 4098 Size: 399 295 225 188 14 770 74 742 Cuts at: 4098 4183 5254 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 770 742 399 295 225 188 85 74 14 BspEI T'CCGG_A Cuts at: 5700 5700 Size: 6037 BspHI T'CATG_A Cuts at: 3926 5656 3926 Size: 1730 4307 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2154 2330 3392 844 Size: 1310 176 1062 3489 Fragments arranged by size: 3489 1310 1062 176 BsrBI CCG'CTC Cuts at: 1394 1823 3924 1394 Size: 429 2101 3507 Fragments arranged by size: 3507 2101 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4532 4706 65 Size: 1183 3284 174 1396 Fragments arranged by size: 3284 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4681 4868 1700 Size: 110 2871 187 2869 Fragments arranged by size: 2871 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6037 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6037 BssSI C'ACGA_G Cuts at: 2309 4097 5109 2309 Size: 1788 1012 3237 Fragments arranged by size: 3237 1788 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3277 794 Size: 2483 3554 BstBI TT'CG_AA Cuts at: 4841 4841 Size: 6037 BstEII G'GTnAC_C Cuts at: 1184 2266 1184 Size: 1082 4955 BstXI CCAn_nnnn'nTGG Cuts at: 2575 2575 Size: 6037 BstYI r'GATC_y Cuts at: 1972 2766 2889 3367 3734 4119 4136 5588 5674 Size: 794 123 478 367 385 17 1452 86 Cuts at: 5674 5686 6032 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 794 478 385 367 346 123 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2562 2562 Size: 6037 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5485 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3152 487 Size: 683 56 1926 3372 Fragments arranged by size: 3372 1926 683 56 BtsI GCAGTG_nn' Cuts at: 2244 3553 4347 4367 2244 Size: 1309 794 20 3914 Fragments arranged by size: 3914 1309 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6037 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6002 DraI TTT'AAA Cuts at: 2091 3130 3341 2091 Size: 1039 211 4787 Fragments arranged by size: 4787 1039 211 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5044 809 Size: 507 3728 1802 Fragments arranged by size: 3728 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3484 4373 6002 Size: 54 1425 50 275 18 1654 889 1629 Cuts at: 6002 8 Size: 43 Fragments arranged by size: 1654 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3484 1537 Size: 275 1672 4090 Fragments arranged by size: 4090 1672 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3967 1353 Size: 2614 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4624 4998 5144 387 Size: 1064 138 3035 374 146 1280 Fragments arranged by size: 3035 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3033 4100 4697 5484 5752 1239 Size: 393 1401 1067 597 787 268 1524 Fragments arranged by size: 1524 1401 1067 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2170 2170 Size: 6037 EcoRI G'AATT_C Cuts at: 2117 3346 3724 2117 Size: 1229 378 4430 Fragments arranged by size: 4430 1229 378 EcoRV GAT'ATC Cuts at: 2350 2350 Size: 6037 FspI TGC'GCA Cuts at: 4543 4543 Size: 6037 HaeII r_GCGC'y Cuts at: 1678 2644 2868 5184 1678 Size: 966 224 2316 2531 Fragments arranged by size: 2531 2316 966 224 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2383 2415 3239 3271 4678 Size: 32 135 32 811 32 824 32 1407 Cuts at: 4678 4710 4752 4784 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1407 824 811 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2762 3381 3627 669 Size: 988 281 824 619 246 3079 Fragments arranged by size: 3079 988 824 619 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6037 HpaI GTT'AAC Cuts at: 3627 3627 Size: 6037 KpnI G_GTAC'C Cuts at: 2131 3362 2131 Size: 1231 4806 MfeI C'AATT_G Cuts at: 3636 3636 Size: 6037 MluI A'CGCG_T Cuts at: 5913 5913 Size: 6037 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5151 5335 1305 Size: 3846 184 2007 Fragments arranged by size: 3846 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4215 Fragments arranged by size: 4215 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2192 2552 2573 2710 3995 4354 4513 Size: 648 1026 360 21 137 1285 359 159 Cuts at: 4513 518 Size: 2042 Fragments arranged by size: 2042 1285 1026 648 360 359 159 137 21 MspA1I CmG'CkG Cuts at: 1606 3453 4130 5278 5523 1606 Size: 1847 677 1148 245 2120 Fragments arranged by size: 2120 1847 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5485 NdeI CA'TA_TG Cuts at: 387 387 Size: 6037 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6037 NotI GC'GGCC_GC Cuts at: 3484 3484 Size: 6037 NspI r_CATG'y Cuts at: 1132 1229 3395 4940 1132 Size: 97 2166 1545 2229 Fragments arranged by size: 2229 2166 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6037 PciI A'CATG_T Cuts at: 4936 4936 Size: 6037 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6037 PfoI T'CCnGG_A Cuts at: 2855 2855 Size: 6037 PmeI GTTT'AAAC Cuts at: 3341 3341 Size: 6037 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4106 4138 5619 5651 4106 Size: 32 1481 32 4492 Fragments arranged by size: 4492 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2170 2170 Size: 6037 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6037 PsiI TTA'TAA Cuts at: 2552 3096 3607 2552 Size: 544 511 4982 Fragments arranged by size: 4982 544 511 PspOMI G'GGCC_C Cuts at: 2508 2508 Size: 6037 PspXI vC'TCGA_Gb Cuts at: 2829 2829 Size: 6037 PstI C_TGCA'G Cuts at: 830 2814 3389 830 Size: 1984 575 3478 Fragments arranged by size: 3478 1984 575 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6037 SalI G'TCGA_C Cuts at: 1936 2760 3379 1936 Size: 824 619 4594 Fragments arranged by size: 4594 824 619 SbfI CC_TGCA'GG Cuts at: 3389 3389 Size: 6037 ScaI AGT'ACT Cuts at: 4285 5997 4285 Size: 1712 4325 SexAI A'CCwGG_T Cuts at: 2360 2360 Size: 6037 SfcI C'TryA_G Cuts at: 826 1045 2402 2784 2810 3385 4520 5201 5392 Size: 219 1357 382 26 575 1135 681 191 Cuts at: 5392 826 Size: 1471 Fragments arranged by size: 1471 1357 1135 681 575 382 219 191 26 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6037 SmaI CCC'GGG Cuts at: 1872 3364 1872 Size: 1492 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2076 2829 4141 5042 5304 820 Size: 197 926 133 753 1312 901 262 1553 Fragments arranged by size: 1553 1312 926 901 753 262 197 133 SnaBI TAC'GTA Cuts at: 493 3828 493 Size: 3335 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6037 SphI G_CATG'C Cuts at: 1132 3395 1132 Size: 2263 3774 SspI AAT'ATT Cuts at: 5 52 2742 3961 5 Size: 47 2690 1219 2081 Fragments arranged by size: 2690 2081 1219 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6037 StyI C'CwwG_G Cuts at: 513 1065 2451 3154 3476 513 Size: 552 1386 703 322 3074 Fragments arranged by size: 3074 1386 703 552 322 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4064 4081 4234 4419 4864 325 Size: 1255 2484 17 153 185 445 1498 Fragments arranged by size: 2484 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4283 5995 96 Size: 274 80 33 51 1443 2306 1712 138 Fragments arranged by size: 2306 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2311 2343 5997 6029 2311 Size: 32 3654 32 2319 Fragments arranged by size: 3654 2319 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2410 1316 Size: 1094 4943 XbaI T'CTAG_A Cuts at: 3373 3373 Size: 6037 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2341 3330 2341 Size: 989 5048 XhoI C'TCGA_G Cuts at: 1943 2829 1943 Size: 886 5151 XmaI C'CCGG_G Cuts at: 1870 3362 1870 Size: 1492 4545 XmnI GAAnn'nnTTC Cuts at: 4166 4166 Size: 6037 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3845 276 Size: 53 83 186 936 2311 2468 Fragments arranged by size: 2468 2311 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI