(Circular) (Six-base) MAPSORT of: pFN21AB7177.seq Check: 6482 from: 1 to: 6151 pFN21AB7177 6151 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3961 278 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3494 1937 Size: 1557 4594 Acc65I G'GTAC_C Cuts at: 3472 3472 Size: 6151 AclI AA'CG_TT Cuts at: 1563 4278 4651 1563 Size: 2715 373 3063 Fragments arranged by size: 3063 2715 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4214 5598 5866 1632 Size: 2582 1384 268 1917 Fragments arranged by size: 2582 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5954 AflIII A'CryG_T Cuts at: 1203 5050 6027 1203 Size: 3847 977 1327 Fragments arranged by size: 3847 1327 977 AgeI A'CCGG_T Cuts at: 4982 4982 Size: 6151 AhdI GACnn_n'nnGTC Cuts at: 4880 4880 Size: 6151 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6151 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6151 AlwNI CAG_nnn'CTG Cuts at: 3894 5466 3894 Size: 1572 4579 ApaI G_GGCC'C Cuts at: 2021 3351 2021 Size: 1330 4821 ApaLI G'TGCA_C Cuts at: 4208 5364 4208 Size: 1156 4995 ApoI r'AATT_y Cuts at: 1493 2687 3460 3686 3838 5947 1493 Size: 1194 773 226 152 2109 1697 Fragments arranged by size: 2109 1697 1194 773 226 152 AseI AT'TA_AT Cuts at: 160 1032 4705 160 Size: 872 3673 1606 Fragments arranged by size: 3673 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 AvaI C'yCGr_G Cuts at: 1870 1943 2021 2134 2269 3476 1870 Size: 73 78 113 135 1207 4545 Fragments arranged by size: 4545 1207 135 113 78 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2309 2342 2309 Size: 33 6118 BamHI G'GATC_C Cuts at: 3481 3848 3481 Size: 367 5784 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2138 2224 2338 3472 4927 Size: 325 217 627 351 86 114 1134 1455 Cuts at: 4927 618 Size: 1842 Fragments arranged by size: 1842 1455 1134 627 351 325 217 114 86 BanII G_rGCy'C Cuts at: 1391 2021 2276 3351 3470 1391 Size: 630 255 1075 119 4072 Fragments arranged by size: 4072 1075 630 255 119 BbeI G_GCGC'C Cuts at: 2142 2228 2142 Size: 86 6065 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2714 3414 928 Size: 971 815 700 3665 Fragments arranged by size: 3665 971 815 700 BbvCI CC'TCA_GC Cuts at: 2152 2173 2152 Size: 21 6130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2296 2330 4342 4376 1903 Size: 34 359 34 2012 34 3678 Fragments arranged by size: 3678 2012 359 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4043 5253 4043 Size: 1210 4941 BclI T'GATC_A Cuts at: 1548 2827 1548 Size: 1279 4872 BfrBI ATG'CAT Cuts at: 3440 3440 Size: 6151 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2099 2363 4762 Size: 107 122 71 631 605 427 264 2399 Cuts at: 4762 136 Size: 1525 Fragments arranged by size: 2399 1525 631 605 427 264 122 107 71 BglII A'GATC_T Cuts at: 6146 6146 Size: 6151 BlpI GC'TnA_GC Cuts at: 3568 3568 Size: 6151 Bme1580I G_kGCm'C Cuts at: 1790 2021 2343 3351 4212 5368 1790 Size: 231 322 1008 861 1156 2573 Fragments arranged by size: 2573 1156 1008 861 322 231 BmgBI CAC'GTC Cuts at: 1318 3226 1318 Size: 1908 4243 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2317 3345 3882 Size: 416 53 83 399 24 900 1028 537 Cuts at: 3882 4840 442 Size: 958 1753 Fragments arranged by size: 1753 1028 958 900 537 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6151 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4811 1239 Size: 3572 2579 Bpu10I CC'TnA_GC Cuts at: 2152 2173 2152 Size: 21 6130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2940 4276 5141 5439 2940 Size: 1336 865 298 3652 Fragments arranged by size: 3652 1336 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2032 3332 4814 882 Size: 617 533 1300 1482 2219 Fragments arranged by size: 2219 1482 1300 617 533 BsaAI yAC'GTr Cuts at: 493 1204 3942 493 Size: 711 2738 2702 Fragments arranged by size: 2738 2702 711 BsaBI GATnn'nnATC Cuts at: 3439 6145 3439 Size: 2706 3445 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2139 2225 3280 3958 Size: 53 83 186 936 606 86 1055 678 Cuts at: 3958 4340 4975 275 Size: 382 635 1451 Fragments arranged by size: 1451 1055 936 678 635 606 382 186 86 83 53 BsaWI w'CCGG_w Cuts at: 4584 4982 5256 5403 5814 4584 Size: 398 274 147 411 4921 Fragments arranged by size: 4921 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5926 Fragments arranged by size: 5926 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2140 2303 2783 1186 Size: 954 163 480 4554 Fragments arranged by size: 4554 954 480 163 BseYI C'CCAG_C Cuts at: 5354 5850 5354 Size: 496 5655 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5578 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 2103 2163 3601 4362 5390 Size: 275 186 60 42 60 1438 761 1028 Cuts at: 5390 1540 Size: 2301 Fragments arranged by size: 2301 1438 1028 761 275 186 60 60 42 BsiHKAI G_wGCw'C Cuts at: 2295 3079 4212 4297 5368 2295 Size: 784 1133 85 1071 3078 Fragments arranged by size: 3078 1133 1071 784 85 BsmI GAATG_Cn' Cuts at: 3662 3755 3662 Size: 93 6058 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2276 2295 2343 3079 3351 3470 Size: 399 231 255 19 48 736 272 119 Cuts at: 3470 4212 4297 5368 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 736 399 272 255 231 119 85 48 19 BspEI T'CCGG_A Cuts at: 5814 5814 Size: 6151 BspHI T'CATG_A Cuts at: 4040 5770 4040 Size: 1730 4421 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3506 844 Size: 2662 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2764 4038 1394 Size: 429 941 1274 3507 Fragments arranged by size: 3507 1274 941 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2451 2457 4646 4820 65 Size: 1183 1203 6 2189 174 1396 Fragments arranged by size: 2189 1396 1203 1183 174 6 BsrFI r'CCGG_y Cuts at: 1700 1810 4795 4982 1700 Size: 110 2985 187 2869 Fragments arranged by size: 2985 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6151 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6151 BssSI C'ACGA_G Cuts at: 4211 5223 4211 Size: 1012 5139 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6151 BstBI TT'CG_AA Cuts at: 4955 4955 Size: 6151 BstEII G'GTnAC_C Cuts at: 1184 2409 1184 Size: 1225 4926 BstXI CCAn_nnnn'nTGG Cuts at: 2577 2577 Size: 6151 BstYI r'GATC_y Cuts at: 1972 3048 3481 3848 4233 4250 5702 5788 5800 Size: 1076 433 367 385 17 1452 86 12 Cuts at: 5800 6146 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1076 433 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2877 2877 Size: 6151 BtgI C'CryG_G Cuts at: 513 1065 2004 3000 513 Size: 552 939 996 3664 Fragments arranged by size: 3664 996 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5412 Fragments arranged by size: 5412 683 56 BtsI GCAGTG_nn' Cuts at: 2124 2988 3667 4461 4481 2124 Size: 864 679 794 20 3794 Fragments arranged by size: 3794 864 794 679 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6151 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6116 DraI TTT'AAA Cuts at: 3455 3455 Size: 6151 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5158 809 Size: 507 3842 1802 Fragments arranged by size: 3842 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2100 2160 Size: 54 1425 50 275 18 228 42 60 Cuts at: 2160 2197 3199 3598 4487 6116 8 Size: 37 1002 399 889 1629 43 Fragments arranged by size: 1629 1425 1002 889 399 275 228 60 54 50 43 42 37 18 EagI C'GGCC_G Cuts at: 1537 1812 2058 2100 2160 3598 1537 Size: 275 246 42 60 1438 4090 Fragments arranged by size: 4090 1438 275 246 60 42 EarI CTCTTCn'nnn_ Cuts at: 1353 2513 2784 3156 3330 3396 4081 1353 Size: 1160 271 372 174 66 685 3423 Fragments arranged by size: 3423 1160 685 372 271 174 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2056 2062 2077 4738 5112 5258 Size: 1064 138 467 6 15 2661 374 146 Cuts at: 5258 387 Size: 1280 Fragments arranged by size: 2661 1280 1064 467 374 146 138 15 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4214 4811 5598 5866 1239 Size: 393 2582 597 787 268 1524 Fragments arranged by size: 2582 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2937 2937 Size: 6151 EcoO109I rG'GnC_Cy Cuts at: 2017 2018 2017 Size: 1 6150 EcoRI G'AATT_C Cuts at: 3460 3838 3460 Size: 378 5773 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3107 3185 3107 Size: 78 6073 FspI TGC'GCA Cuts at: 4657 4657 Size: 6151 HaeII r_GCGC'y Cuts at: 1678 2142 2228 5298 1678 Size: 464 86 3070 2531 Fragments arranged by size: 3070 2531 464 86 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2860 2892 4792 4824 4866 Size: 32 135 32 1288 32 1900 32 42 Cuts at: 4866 4898 1373 Size: 32 2626 Fragments arranged by size: 2626 1900 1288 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3495 3741 669 Size: 988 281 1557 246 3079 Fragments arranged by size: 3079 1557 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6151 HpaI GTT'AAC Cuts at: 3741 3741 Size: 6151 KasI G'GCGC_C Cuts at: 2138 2224 2138 Size: 86 6065 KpnI G_GTAC'C Cuts at: 3476 3476 Size: 6151 MfeI C'AATT_G Cuts at: 3750 3750 Size: 6151 MluI A'CGCG_T Cuts at: 6027 6027 Size: 6151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2296 2421 2671 3131 5265 5449 1305 Size: 991 125 250 460 2134 184 2007 Fragments arranged by size: 2134 2007 991 460 250 184 125 MscI TGG'CCA Cuts at: 10 64 1489 1832 2199 10 Size: 54 1425 343 367 3962 Fragments arranged by size: 3962 1425 367 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3392 4109 4468 4627 518 Size: 648 2226 717 359 159 2042 Fragments arranged by size: 2226 2042 717 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3567 4244 5392 5637 1606 Size: 1961 677 1148 245 2120 Fragments arranged by size: 2120 1961 1148 677 245 NarI GG'CG_CC Cuts at: 2139 2225 2139 Size: 86 6065 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4660 Fragments arranged by size: 4660 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6151 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6151 NotI GC'GGCC_GC Cuts at: 2058 3598 2058 Size: 1540 4611 NsiI A_TGCA'T Cuts at: 3442 3442 Size: 6151 NspI r_CATG'y Cuts at: 1132 1229 2401 3509 5054 1132 Size: 97 1172 1108 1545 2229 Fragments arranged by size: 2229 1545 1172 1108 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6151 PciI A'CATG_T Cuts at: 5050 5050 Size: 6151 PflMI CCAn_nnn'nTGG Cuts at: 1108 2335 1108 Size: 1227 4924 PmeI GTTT'AAAC Cuts at: 3455 3455 Size: 6151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4220 4252 5733 5765 4220 Size: 32 1481 32 4606 Fragments arranged by size: 4606 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6151 PsiI TTA'TAA Cuts at: 3721 3721 Size: 6151 PspOMI G'GGCC_C Cuts at: 2017 3347 2017 Size: 1330 4821 PstI C_TGCA'G Cuts at: 830 3503 830 Size: 2673 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6151 SalI G'TCGA_C Cuts at: 1936 3493 1936 Size: 1557 4594 SapI GCTCTTCn'nnn_ Cuts at: 2513 3156 2513 Size: 643 5508 SbfI CC_TGCA'GG Cuts at: 3503 3503 Size: 6151 ScaI AGT'ACT Cuts at: 4399 6111 4399 Size: 1712 4439 SfcI C'TryA_G Cuts at: 826 1045 2670 3156 3499 4634 5315 5506 826 Size: 219 1625 486 343 1135 681 191 1471 Fragments arranged by size: 1625 1471 1135 681 486 343 219 191 SfoI GGC'GCC Cuts at: 2140 2226 2140 Size: 86 6065 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6151 SmaI CCC'GGG Cuts at: 1872 3478 1872 Size: 1606 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2955 4255 5156 5418 820 Size: 197 926 1012 1300 901 262 1553 Fragments arranged by size: 1553 1300 1012 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3942 493 Size: 3449 2702 SpeI A'CTAG_T Cuts at: 152 2723 152 Size: 2571 3580 SphI G_CATG'C Cuts at: 1132 3509 1132 Size: 2377 3774 SspI AAT'ATT Cuts at: 5 52 3058 4075 5 Size: 47 3006 1017 2081 Fragments arranged by size: 3006 2081 1017 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6151 StyI C'CwwG_G Cuts at: 513 1065 2004 3590 513 Size: 552 939 1586 3074 Fragments arranged by size: 3074 1586 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2428 4178 4195 4348 4533 4978 325 Size: 1255 848 1750 17 153 185 445 1498 Fragments arranged by size: 1750 1498 1255 848 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4397 6109 96 Size: 274 80 33 51 1443 2420 1712 138 Fragments arranged by size: 2420 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6111 6143 6111 Size: 32 6119 Tth111I GACn'n_nGTC Cuts at: 1316 2373 1316 Size: 1057 5094 XbaI T'CTAG_A Cuts at: 3487 3487 Size: 6151 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6151 XmaI C'CCGG_G Cuts at: 1870 3476 1870 Size: 1606 4545 XmnI GAAnn'nnTTC Cuts at: 4280 4280 Size: 6151 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3959 276 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI