(Circular) (Six-base) MAPSORT of: pFN21AB7161.seq Check: 8063 from: 1 to: 5824 pFN21AB7161 5824 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3167 1937 Size: 1230 4594 Acc65I G'GTAC_C Cuts at: 3145 3145 Size: 5824 AclI AA'CG_TT Cuts at: 1563 2037 3951 4324 1563 Size: 474 1914 373 3063 Fragments arranged by size: 3063 1914 474 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3887 5271 5539 1632 Size: 2255 1384 268 1917 Fragments arranged by size: 2255 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 4723 5700 1203 Size: 3520 977 1327 Fragments arranged by size: 3520 1327 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 2404 4553 2404 Size: 2149 3675 AleI CACnn'nnGTG Cuts at: 1166 2619 1166 Size: 1453 4371 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5824 AlwNI CAG_nnn'CTG Cuts at: 2392 3567 5139 2392 Size: 1175 1572 3077 Fragments arranged by size: 3077 1572 1175 ApaI G_GGCC'C Cuts at: 2609 2609 Size: 5824 ApaLI G'TGCA_C Cuts at: 3881 5037 3881 Size: 1156 4668 ApoI r'AATT_y Cuts at: 1493 2897 3133 3359 3511 5620 1493 Size: 1404 236 226 152 2109 1697 Fragments arranged by size: 2109 1697 1404 236 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 2678 3149 1870 Size: 73 735 471 4545 Fragments arranged by size: 4545 735 471 73 AvrII C'CTAG_G Cuts at: 2813 2813 Size: 5824 BamHI G'GATC_C Cuts at: 3154 3521 3154 Size: 367 5457 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2215 2378 2869 3145 4600 Size: 325 217 627 428 163 491 276 1455 Cuts at: 4600 618 Size: 1842 Fragments arranged by size: 1842 1455 627 491 428 325 276 217 163 BanII G_rGCy'C Cuts at: 1391 2309 2336 2560 2609 2823 2920 3143 1391 Size: 918 27 224 49 214 97 223 4072 Fragments arranged by size: 4072 918 224 223 214 97 49 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2079 928 Size: 971 180 4673 Fragments arranged by size: 4673 971 180 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4015 4049 1903 Size: 34 50 34 1994 34 3678 Fragments arranged by size: 3678 1994 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2365 3716 4926 2365 Size: 1351 1210 3263 Fragments arranged by size: 3263 1351 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4435 136 Size: 107 122 71 631 605 2763 1525 Fragments arranged by size: 2763 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 3241 3241 Size: 5824 Bme1580I G_kGCm'C Cuts at: 1790 2220 2609 2874 3885 5041 1790 Size: 430 389 265 1011 1156 2573 Fragments arranged by size: 2573 1156 1011 430 389 265 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5824 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2086 2250 3555 Size: 416 53 83 399 24 669 164 1305 Cuts at: 3555 4513 442 Size: 958 1753 Fragments arranged by size: 1753 1305 958 669 416 399 164 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2137 2236 2137 Size: 99 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2876 4484 1239 Size: 1637 1608 2579 Fragments arranged by size: 2579 1637 1608 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2534 3949 4814 5112 2534 Size: 1415 865 298 3246 Fragments arranged by size: 3246 1415 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4487 882 Size: 617 2988 2219 Fragments arranged by size: 2988 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3615 493 Size: 711 2411 2702 Fragments arranged by size: 2702 2411 711 BsaBI GATnn'nnATC Cuts at: 2516 5818 2516 Size: 3302 2522 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3631 4013 4648 275 Size: 53 83 186 936 2098 382 635 1451 Fragments arranged by size: 2098 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4257 4655 4929 5076 5487 4257 Size: 398 274 147 411 4594 Fragments arranged by size: 4594 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2033 2063 1178 Size: 30 165 30 630 30 4939 Fragments arranged by size: 4939 630 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2113 2126 2219 2222 2225 2308 2360 2453 Size: 927 13 93 3 3 83 52 93 Cuts at: 2453 2495 2756 2813 2874 1186 Size: 42 261 57 61 4136 Fragments arranged by size: 4136 927 261 93 93 83 61 57 52 42 13 3 3 BseYI C'CCAG_C Cuts at: 2783 5027 5523 2783 Size: 2244 496 3084 Fragments arranged by size: 3084 2244 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2861 1163 Size: 573 1125 4126 Fragments arranged by size: 4126 1125 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 4035 5063 1540 Size: 275 186 1273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2309 2336 2796 2823 3885 3970 5041 2309 Size: 27 460 27 1062 85 1071 3092 Fragments arranged by size: 3092 1071 1062 460 85 27 27 BsmI GAATG_Cn' Cuts at: 2250 2770 3335 3428 2250 Size: 520 565 93 4646 Fragments arranged by size: 4646 565 520 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3115 3115 Size: 5824 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2220 2309 2336 2560 2609 2796 2823 Size: 399 430 89 27 224 49 187 27 Cuts at: 2823 2874 2920 3143 3885 3970 5041 1391 Size: 51 46 223 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 430 399 224 223 187 89 85 51 49 46 27 27 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3179 844 Size: 2335 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3711 1394 Size: 429 1888 3507 Fragments arranged by size: 3507 1888 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4319 4493 65 Size: 1183 3071 174 1396 Fragments arranged by size: 3071 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4468 4655 1700 Size: 110 2658 187 2869 Fragments arranged by size: 2869 2658 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 3884 4896 3884 Size: 1012 4812 BstAPI GCAn_nnn'nTGC Cuts at: 794 3062 794 Size: 2268 3556 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5824 BstXI CCAn_nnnn'nTGG Cuts at: 3073 3073 Size: 5824 BstYI r'GATC_y Cuts at: 1972 3154 3521 3906 3923 5375 5461 5473 5819 Size: 1182 367 385 17 1452 86 12 346 Cuts at: 5819 1972 Size: 1977 Fragments arranged by size: 1977 1452 1182 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2202 2202 Size: 5824 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5272 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5085 Fragments arranged by size: 5085 683 56 BtsI GCAGTG_nn' Cuts at: 3340 4134 4154 3340 Size: 794 20 5010 Fragments arranged by size: 5010 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5789 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4831 809 Size: 507 3515 1802 Fragments arranged by size: 3515 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2439 3075 3271 Size: 54 1425 50 275 18 609 636 196 Cuts at: 3271 4160 5789 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 636 609 275 196 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2103 2137 2167 2185 3021 3754 1353 Size: 750 34 30 18 836 733 3423 Fragments arranged by size: 3423 836 750 733 34 30 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2116 4411 4785 4931 387 Size: 1064 138 527 2295 374 146 1280 Fragments arranged by size: 2295 1280 1064 527 374 146 138 EcoICRI GAG'CTC Cuts at: 2307 2334 2821 2307 Size: 27 487 5310 Fragments arranged by size: 5310 487 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2876 3887 4484 5271 5539 1239 Size: 393 1244 1011 597 787 268 1524 Fragments arranged by size: 1524 1244 1011 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2273 2285 2389 2606 2273 Size: 12 104 217 5491 Fragments arranged by size: 5491 217 104 12 EcoRI G'AATT_C Cuts at: 2897 3133 3511 2897 Size: 236 378 5210 Fragments arranged by size: 5210 378 236 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 4971 1678 Size: 3293 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 2221 2253 2390 Size: 32 135 32 461 32 156 32 137 Cuts at: 2390 2422 4465 4497 4539 4571 1373 Size: 32 2043 32 42 32 2626 Fragments arranged by size: 2626 2043 461 156 137 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2041 3168 3414 669 Size: 988 281 103 1127 246 3079 Fragments arranged by size: 3079 1127 988 281 246 103 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 3149 3149 Size: 5824 MfeI C'AATT_G Cuts at: 3045 3423 3045 Size: 378 5446 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4938 5122 1305 Size: 3633 184 2007 Fragments arranged by size: 3633 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3077 10 Size: 54 1425 343 1245 2757 Fragments arranged by size: 2757 1425 1245 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2619 3071 3782 4141 4300 518 Size: 648 1453 452 711 359 159 2042 Fragments arranged by size: 2042 1453 711 648 452 359 159 MspA1I CmG'CkG Cuts at: 1606 3240 3917 5065 5310 1606 Size: 1634 677 1148 245 2120 Fragments arranged by size: 2120 1634 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5272 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 2677 3182 4727 1132 Size: 97 1448 505 1545 2229 Fragments arranged by size: 2229 1545 1448 505 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5824 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5824 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3893 3925 5406 5438 3893 Size: 32 1481 32 4279 Fragments arranged by size: 4279 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2273 2285 2273 Size: 12 5812 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PspOMI G'GGCC_C Cuts at: 2605 2605 Size: 5824 PstI C_TGCA'G Cuts at: 830 3176 830 Size: 2346 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 SacI G_AGCT'C Cuts at: 2309 2336 2823 2309 Size: 27 487 5310 Fragments arranged by size: 5310 487 27 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 2946 4072 5784 2946 Size: 1126 1712 2986 Fragments arranged by size: 2986 1712 1126 SfcI C'TryA_G Cuts at: 826 1045 3172 4307 4988 5179 826 Size: 219 2127 1135 681 191 1471 Fragments arranged by size: 2127 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 3151 1872 Size: 1279 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2549 3928 4829 5091 820 Size: 197 926 606 1379 901 262 1553 Fragments arranged by size: 1553 1379 926 901 606 262 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 2677 3182 1132 Size: 1545 505 3774 Fragments arranged by size: 3774 1545 505 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 2643 1517 Size: 1126 4698 StyI C'CwwG_G Cuts at: 513 1065 2813 3263 513 Size: 552 1748 450 3074 Fragments arranged by size: 3074 1748 552 450 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2827 3851 3868 4021 4206 4651 325 Size: 1255 1247 1024 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1247 1024 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2142 2523 2944 Size: 274 80 33 51 1443 165 381 421 Cuts at: 2944 4070 5782 96 Size: 1126 1712 138 Fragments arranged by size: 1712 1443 1126 421 381 274 165 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5784 5816 5784 Size: 32 5792 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 3149 1870 Size: 1279 4545 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI