Restriction map for
pFN21AB7157
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AflIII AgeI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AviII BamHI
BanII BbeI BbrPI BbvCI BciVI
BclI BcnI BfrI BsaAI BsaBI
BsaHI BsaI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MseI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfoI SfuI SgrAI SmaI
SmlI SnaBI Sse8387I SspBI SspI
SwaI TaqI Tru9I TthHB8I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 607 TCCGGA T^CCGGA
AcsI 276 GAATTT [GA]^AATT[CT]
AhdI 516 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 117 GTGCAC G^TGCAC
ApaLI 117 GTGCAC G^TGCAC
ApoI 276 GAATTT [GA]^AATT[CT]
AspEI 516 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 823 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 996 CCCGAG C^[CT]CG[GA]G
AvrII 738 CCTAGG C^CTAGG
BanI 690 GGTGCC G^G[CT][GA]CC
BcgI 391 CGATACCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 787 GCCCTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1059 AGATCT A^GATCT
BlnI 738 CCTAGG C^CTAGG
BlpI 573 GCTGAGC GC^T[ACGT]AGC
BpmI 145 CTGGAG CTGGAG(16/14)
Bpu1102I 573 GCTGAGC GC^T[ACGT]AGC
BsaWI 607 TCCGGA [AT]^CCGG[AT]
BseAI 607 TCCGGA T^CCGGA
BsmI 7 GAATGC GAATGC(1/-1)
BsoBI 996 CCCGAG C^[CT]CG[GA]G
BspEI 607 TCCGGA T^CCGGA
BsrBI 918 CCGCTC CCGCTC(-3/-3)
BstEII 453 GGTGACC G^GT[ACGT]ACC
BstPI 453 GGTGACC G^GT[ACGT]ACC
CelII 573 GCTGAGC GC^T[ACGT]AGC
CfoI 405 GCGC GCG^C
DraIII 1038 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 516 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 122 CTCTTC CTCTTC(1/4)
EcoO65I 453 GGTGACC G^GT[ACGT]ACC
HhaI 405 GCGC GCG^C
HinP1I 405 GCGC G^CGC
HincII 880 GTCAAC GT[CT]^[GA]AC
HindII 880 GTCAAC GT[CT]^[GA]AC
Ksp632I 122 CTCTTC CTCTTC(1/4)
MaeII 152 ACGT A^CGT
MroI 607 TCCGGA T^CCGGA
MslI 649 CATCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 101 CAGCTG C[AC]G^C[GT]G
PflFI 823 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 473 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 101 CAGCTG CAG^CTG
SexAI 824 ACCAGGT A^CC[AT]GGT
SfaNI 464 GCATC GCATC(5/9)
SfiI 786 GGCCCTCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 486 ACTAGT A^CTAGT
SphI 262 GCATGC GCATG^C
StuI 836 AGGCCT AGG^CCT
Tsp509I 277 AATT ^AATT
Tth111I 823 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1062 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 13 enzymes) #
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AvaII 88 GGTCC G^G[AT]CC
474 GGTCC
BbsI 96 GAAGAC GAAGAC(2/6)
313 GTCTTC
BpuAI 96 GAAGAC GAAGAC(2/6)
313 GTCTTC
BseRI 760 CTCCTC GAGGAG(10/8)
921 CTCCTC
BsmAI 1015 GAGAC GTCTC(1/5)
490 GTCTC
BsmFI 89 GTCCC GGGAC(10/14)
524 GTCCC
HapII 195 CCGG C^CGG
608 CCGG
HpaII 195 CCGG C^CGG
608 CCGG
MspI 195 CCGG C^CGG
608 CCGG
NcoI 291 CCATGG C^CATGG
875 CCATGG
PflMI 291 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
798 CCACAGCCTGG
Van91I 291 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
798 CCACAGCCTGG
XcmI 38 CCAAGGATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
282 CCATGTTTTCCATGG
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
BalI BbvI BfaI BmyI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MvaI
MwoI NdeII NlaIII NlaIV NspI
Sau3AI Sau96I ScrFI StyI TfiI
TseI Tsp45I TspRI XhoII