(Circular) (Six-base) MAPSORT of: pFN21AB7157.seq Check: 2942 from: 1 to: 5785 pFN21AB7157 5785 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2683 2683 Size: 5785 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3595 278 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3128 1937 Size: 1191 4594 Acc65I G'GTAC_C Cuts at: 3106 3106 Size: 5785 AclI AA'CG_TT Cuts at: 1563 3912 4285 1563 Size: 2349 373 3063 Fragments arranged by size: 3063 2349 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2348 3848 5232 5500 1632 Size: 716 1500 1384 268 1917 Fragments arranged by size: 1917 1500 1384 716 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5588 AflIII A'CryG_T Cuts at: 1203 4684 5661 1203 Size: 3481 977 1327 Fragments arranged by size: 3481 1327 977 AgeI A'CCGG_T Cuts at: 4616 4616 Size: 5785 AhdI GACnn_n'nnGTC Cuts at: 2526 4514 2526 Size: 1988 3797 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5785 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5785 AlwNI CAG_nnn'CTG Cuts at: 2363 2538 3029 3528 5100 2363 Size: 175 491 499 1572 3048 Fragments arranged by size: 3048 1572 499 491 175 ApaLI G'TGCA_C Cuts at: 2122 3842 4998 2122 Size: 1720 1156 2909 Fragments arranged by size: 2909 1720 1156 ApoI r'AATT_y Cuts at: 1493 2281 3094 3320 3472 5581 1493 Size: 788 813 226 152 2109 1697 Fragments arranged by size: 2109 1697 813 788 226 152 AseI AT'TA_AT Cuts at: 160 1032 4339 160 Size: 872 3307 1606 Fragments arranged by size: 3307 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 AvaI C'yCGr_G Cuts at: 1870 1943 3001 3110 1870 Size: 73 1058 109 4545 Fragments arranged by size: 4545 1058 109 73 AvrII C'CTAG_G Cuts at: 2743 2743 Size: 5785 BamHI G'GATC_C Cuts at: 3115 3482 3115 Size: 367 5418 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2695 3106 4561 618 Size: 325 217 627 908 411 1455 1842 Fragments arranged by size: 1842 1455 908 627 411 325 217 BanII G_rGCy'C Cuts at: 1391 3104 1391 Size: 1713 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2108 2311 928 Size: 971 209 203 4402 Fragments arranged by size: 4402 971 209 203 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2385 2419 3976 4010 1903 Size: 34 448 34 1557 34 3678 Fragments arranged by size: 3678 1557 448 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3677 4887 3677 Size: 1210 4575 BclI T'GATC_A Cuts at: 1548 1548 Size: 5785 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2798 4396 136 Size: 107 122 71 631 605 1126 1598 1525 Fragments arranged by size: 1598 1525 1126 631 605 122 107 71 BglII A'GATC_T Cuts at: 3064 5780 3064 Size: 2716 3069 BlpI GC'TnA_GC Cuts at: 2579 3202 2579 Size: 623 5162 Bme1580I G_kGCm'C Cuts at: 1790 2126 2149 2455 3846 5002 1790 Size: 336 23 306 1391 1156 2573 Fragments arranged by size: 2573 1391 1156 336 306 23 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5785 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2777 3516 4474 Size: 416 53 83 399 24 1360 739 958 Cuts at: 4474 442 Size: 1753 Fragments arranged by size: 1753 1360 958 739 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5785 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2941 2941 Size: 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 4445 1239 Size: 932 2274 2579 Fragments arranged by size: 2579 2274 932 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3910 4775 5073 3910 Size: 865 298 4622 Fragments arranged by size: 4622 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4448 882 Size: 617 2949 2219 Fragments arranged by size: 2949 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3576 493 Size: 711 2372 2702 Fragments arranged by size: 2702 2372 711 BsaBI GATnn'nnATC Cuts at: 5779 5779 Size: 5785 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3592 3974 4609 275 Size: 53 83 186 936 2059 382 635 1451 Fragments arranged by size: 2059 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2612 4218 4616 4890 5037 5448 2612 Size: 1606 398 274 147 411 2949 Fragments arranged by size: 2949 1606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5560 Fragments arranged by size: 5560 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2756 2917 1186 Size: 1570 161 4054 Fragments arranged by size: 4054 1570 161 BseYI C'CCAG_C Cuts at: 2016 2234 4988 5484 2016 Size: 218 2754 496 2317 Fragments arranged by size: 2754 2317 496 218 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3235 3996 5024 1540 Size: 275 186 1234 761 1028 2301 Fragments arranged by size: 2301 1234 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2126 2169 2766 3846 3931 5002 2126 Size: 43 597 1080 85 1071 2909 Fragments arranged by size: 2909 1080 1071 597 85 43 BsmI GAATG_Cn' Cuts at: 2018 3296 3389 2018 Size: 1278 93 4414 Fragments arranged by size: 4414 1278 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2126 2149 2169 2455 2766 3104 3846 Size: 399 336 23 20 286 311 338 742 Cuts at: 3846 3931 5002 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 338 336 311 286 85 23 20 BspEI T'CCGG_A Cuts at: 2612 5448 2612 Size: 2836 2949 BspHI T'CATG_A Cuts at: 3674 5404 3674 Size: 1730 4055 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2511 2583 2683 3140 844 Size: 1667 72 100 457 3489 Fragments arranged by size: 3489 1667 457 100 72 BsrBI CCG'CTC Cuts at: 1394 1823 2925 3672 1394 Size: 429 1102 747 3507 Fragments arranged by size: 3507 1102 747 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4280 4454 65 Size: 1183 3032 174 1396 Fragments arranged by size: 3032 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4429 4616 1700 Size: 110 2619 187 2869 Fragments arranged by size: 2869 2619 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5785 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5785 BssSI C'ACGA_G Cuts at: 3845 4857 3845 Size: 1012 4773 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5785 BstBI TT'CG_AA Cuts at: 4589 4589 Size: 5785 BstEII G'GTnAC_C Cuts at: 1184 2458 1184 Size: 1274 4511 BstYI r'GATC_y Cuts at: 1972 2919 3005 3064 3115 3482 3867 3884 5336 Size: 947 86 59 51 367 385 17 1452 Cuts at: 5336 5422 5434 5780 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 947 385 367 346 86 86 59 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2296 2443 2880 3047 513 Size: 552 939 292 147 437 167 3251 Fragments arranged by size: 3251 939 552 437 292 167 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2581 487 Size: 683 56 1355 3691 Fragments arranged by size: 3691 1355 683 56 BtsI GCAGTG_nn' Cuts at: 2938 3301 4095 4115 2938 Size: 363 794 20 4608 Fragments arranged by size: 4608 794 363 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5785 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5750 DraI TTT'AAA Cuts at: 3089 3089 Size: 5785 DraIII CAC_nnn'GTG Cuts at: 3048 3048 Size: 5785 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4792 809 Size: 507 3476 1802 Fragments arranged by size: 3476 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2664 2677 Size: 54 1425 50 275 18 420 414 13 Cuts at: 2677 2733 2811 3050 3232 4121 5750 8 Size: 56 78 239 182 889 1629 43 Fragments arranged by size: 1629 1425 889 420 414 275 239 182 78 56 54 50 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 3232 1537 Size: 275 1420 4090 Fragments arranged by size: 4090 1420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2133 3715 1353 Size: 780 1582 3423 Fragments arranged by size: 3423 1582 780 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2186 4372 4746 4892 387 Size: 1064 138 597 2186 374 146 1280 Fragments arranged by size: 2186 1280 1064 597 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2348 3848 4445 5232 5500 1239 Size: 393 539 177 1500 597 787 268 1524 Fragments arranged by size: 1524 1500 787 597 539 393 268 177 EcoO109I rG'GnC_Cy Cuts at: 2479 2535 2971 2479 Size: 56 436 5293 Fragments arranged by size: 5293 436 56 EcoRI G'AATT_C Cuts at: 3094 3472 3094 Size: 378 5407 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2068 2335 2860 2068 Size: 267 525 4993 Fragments arranged by size: 4993 525 267 FspI TGC'GCA Cuts at: 4291 4291 Size: 5785 HaeII r_GCGC'y Cuts at: 1678 4932 1678 Size: 3254 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2512 2544 2997 3029 4426 Size: 32 135 32 940 32 453 32 1397 Cuts at: 4426 4458 4500 4532 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1397 940 453 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2887 3129 3375 669 Size: 988 281 949 242 246 3079 Fragments arranged by size: 3079 988 949 281 246 242 HindIII A'AGCT_T Cuts at: 748 748 Size: 5785 HpaI GTT'AAC Cuts at: 3375 3375 Size: 5785 KpnI G_GTAC'C Cuts at: 3110 3110 Size: 5785 MfeI C'AATT_G Cuts at: 3384 3384 Size: 5785 MluI A'CGCG_T Cuts at: 5661 5661 Size: 5785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2085 4899 5083 1305 Size: 780 2814 184 2007 Fragments arranged by size: 2814 2007 780 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2252 2666 2679 2735 2813 Size: 54 1425 343 420 414 13 56 78 Cuts at: 2813 3052 10 Size: 239 2743 Fragments arranged by size: 2743 1425 420 414 343 239 78 56 54 13 MslI CAynn'nnrTG Cuts at: 518 1166 2658 3743 4102 4261 518 Size: 648 1492 1085 359 159 2042 Fragments arranged by size: 2042 1492 1085 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2108 3201 3878 5026 5271 1606 Size: 502 1093 677 1148 245 2120 Fragments arranged by size: 2120 1148 1093 677 502 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2296 2880 513 Size: 552 939 292 584 3418 Fragments arranged by size: 3418 939 584 552 292 NdeI CA'TA_TG Cuts at: 387 387 Size: 5785 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5785 NotI GC'GGCC_GC Cuts at: 3232 3232 Size: 5785 NspI r_CATG'y Cuts at: 1132 1229 2271 2639 2663 3143 4688 1132 Size: 97 1042 368 24 480 1545 2229 Fragments arranged by size: 2229 1545 1042 480 368 97 24 PasI CC'CwG_GG Cuts at: 1348 2464 1348 Size: 1116 4669 PciI A'CATG_T Cuts at: 4684 4684 Size: 5785 PflMI CCAn_nnn'nTGG Cuts at: 1108 2302 2809 1108 Size: 1194 507 4084 Fragments arranged by size: 4084 1194 507 PmeI GTTT'AAAC Cuts at: 3089 3089 Size: 5785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3854 3886 5367 5399 3854 Size: 32 1481 32 4240 Fragments arranged by size: 4240 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2479 2479 Size: 5785 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5785 PsiI TTA'TAA Cuts at: 3355 3355 Size: 5785 PstI C_TGCA'G Cuts at: 830 3137 830 Size: 2307 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5785 PvuII CAG'CTG Cuts at: 2108 2108 Size: 5785 SalI G'TCGA_C Cuts at: 1936 3127 1936 Size: 1191 4594 SbfI CC_TGCA'GG Cuts at: 3137 3137 Size: 5785 ScaI AGT'ACT Cuts at: 4033 5745 4033 Size: 1712 4073 SexAI A'CCwGG_T Cuts at: 2829 2829 Size: 5785 SfcI C'TryA_G Cuts at: 826 1045 3133 4268 4949 5140 826 Size: 219 2088 1135 681 191 1471 Fragments arranged by size: 2088 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2798 2798 Size: 5785 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5785 SmaI CCC'GGG Cuts at: 1872 3112 1872 Size: 1240 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3889 4790 5052 820 Size: 197 926 1946 901 262 1553 Fragments arranged by size: 1946 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3576 493 Size: 3083 2702 SpeI A'CTAG_T Cuts at: 152 2491 152 Size: 2339 3446 SphI G_CATG'C Cuts at: 1132 2271 3143 1132 Size: 1139 872 3774 Fragments arranged by size: 3774 1139 872 SspI AAT'ATT Cuts at: 5 52 3709 5 Size: 47 3657 2081 Fragments arranged by size: 3657 2081 47 StuI AGG'CCT Cuts at: 1517 2843 1517 Size: 1326 4459 StyI C'CwwG_G Cuts at: 513 1065 2004 2043 2296 2310 2743 2880 2974 Size: 552 939 39 253 14 433 137 94 Cuts at: 2974 3224 513 Size: 250 3074 Fragments arranged by size: 3074 939 552 433 253 250 137 94 39 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2008 2409 3812 3829 3982 4167 4612 Size: 1255 428 401 1403 17 153 185 445 Cuts at: 4612 325 Size: 1498 Fragments arranged by size: 1498 1403 1255 445 428 401 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4031 5743 96 Size: 274 80 33 51 1443 2054 1712 138 Fragments arranged by size: 2054 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2947 2979 5745 5777 2947 Size: 32 2766 32 2955 Fragments arranged by size: 2955 2766 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2831 1316 Size: 1515 4270 XbaI T'CTAG_A Cuts at: 3067 3121 3067 Size: 54 5731 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2050 2294 2050 Size: 244 5541 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5785 XmaI C'CCGG_G Cuts at: 1870 3110 1870 Size: 1240 4545 XmnI GAAnn'nnTTC Cuts at: 3914 3914 Size: 5785 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3593 276 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfoI SrfI SwaI