Restriction map for
pFN21AB7157
(Linear) (Six-base) MAPSORT of: KIBB7157.seq Check: 9808 from: 1 to: 1080
KIBB7157.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 678 1080
Size: 678 402
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 1080
Size: 343 737
AhdI GACnn_n'nnGTC
Cuts at: 0 521 1080
Size: 521 559
AlwNI CAG_nnn'CTG
Cuts at: 0 358 533 1024 1080
Size: 358 175 491 56
Fragments arranged by size:
491 358 175 56
ApaLI G'TGCA_C
Cuts at: 0 117 1080
Size: 117 963
ApoI r'AATT_y
Cuts at: 0 276 1080
Size: 276 804
AvaI C'yCGr_G
Cuts at: 0 996 1080
Size: 996 84
AvrII C'CTAG_G
Cuts at: 0 738 1080
Size: 738 342
BanI G'GyrC_C
Cuts at: 0 690 1080
Size: 690 390
BbsI GAAGACnn'nnnn_
Cuts at: 0 103 306 1080
Size: 103 203 774
Fragments arranged by size:
774 203 103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 380 414 1080
Size: 380 34 666
Fragments arranged by size:
666 380 34
BglI GCCn_nnn'nGGC
Cuts at: 0 793 1080
Size: 793 287
BglII A'GATC_T
Cuts at: 0 1059 1080
Size: 1059 21
BlpI GC'TnA_GC
Cuts at: 0 574 1080
Size: 574 506
Bme1580I G_kGCm'C
Cuts at: 0 121 144 450 1080
Size: 121 23 306 630
Fragments arranged by size:
630 306 121 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 772 1080
Size: 772 308
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 936 1080
Size: 936 144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1080
Size: 166 914
BsaWI w'CCGG_w
Cuts at: 0 607 1080
Size: 607 473
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 751 912 1080
Size: 751 161 168
Fragments arranged by size:
751 168 161
BseYI C'CCAG_C
Cuts at: 0 11 229 1080
Size: 11 218 851
Fragments arranged by size:
851 218 11
BsiHKAI G_wGCw'C
Cuts at: 0 121 164 761 1080
Size: 121 43 597 319
Fragments arranged by size:
597 319 121 43
BsmI GAATG_Cn'
Cuts at: 0 13 1080
Size: 13 1067
Bsp1286I G_dGCh'C
Cuts at: 0 121 144 164 450 761 1080
Size: 121 23 20 286 311 319
Fragments arranged by size:
319 311 286 121 23 20
BspEI T'CCGG_A
Cuts at: 0 607 1080
Size: 607 473
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 506 578 678 1080
Size: 506 72 100 402
Fragments arranged by size:
506 402 100 72
BsrBI CCG'CTC
Cuts at: 0 920 1080
Size: 920 160
BstEII G'GTnAC_C
Cuts at: 0 453 1080
Size: 453 627
BstYI r'GATC_y
Cuts at: 0 914 1000 1059 1080
Size: 914 86 59 21
Fragments arranged by size:
914 86 59 21
BtgI C'CryG_G
Cuts at: 0 291 438 875 1042 1080
Size: 291 147 437 167 38
Fragments arranged by size:
437 291 167 147 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 576 1080
Size: 576 504
BtsI GCAGTG_nn'
Cuts at: 0 933 1080
Size: 933 147
DraIII CAC_nnn'GTG
Cuts at: 0 1043 1080
Size: 1043 37
EaeI y'GGCC_r
Cuts at: 0 245 659 672 728 806 1045 1080
Size: 245 414 13 56 78 239 35
Fragments arranged by size:
414 245 239 78 56 35 13
EarI CTCTTCn'nnn_
Cuts at: 0 128 1080
Size: 128 952
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 181 1080
Size: 181 899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 343 1080
Size: 166 177 737
Fragments arranged by size:
737 177 166
EcoO109I rG'GnC_Cy
Cuts at: 0 474 530 966 1080
Size: 474 56 436 114
Fragments arranged by size:
474 436 114 56
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 63 330 855 1080
Size: 63 267 525 225
Fragments arranged by size:
525 267 225 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 507 539 992 1024 1080
Size: 507 32 453 32 56
Fragments arranged by size:
507 453 56 32 32
HincII GTy'rAC
Cuts at: 0 882 1080
Size: 882 198
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 1080
Size: 80 1000
MscI TGG'CCA
Cuts at: 0 247 661 674 730 808 1047 1080
Size: 247 414 13 56 78 239 33
Fragments arranged by size:
414 247 239 78 56 33 13
MslI CAynn'nnrTG
Cuts at: 0 653 1080
Size: 653 427
MspA1I CmG'CkG
Cuts at: 0 103 1080
Size: 103 977
NcoI C'CATG_G
Cuts at: 0 291 875 1080
Size: 291 584 205
Fragments arranged by size:
584 291 205
NspI r_CATG'y
Cuts at: 0 266 634 658 1080
Size: 266 368 24 422
Fragments arranged by size:
422 368 266 24
PasI CC'CwG_GG
Cuts at: 0 459 1080
Size: 459 621
PflMI CCAn_nnn'nTGG
Cuts at: 0 297 804 1080
Size: 297 507 276
Fragments arranged by size:
507 297 276
PpuMI rG'GwC_Cy
Cuts at: 0 474 1080
Size: 474 606
PvuII CAG'CTG
Cuts at: 0 103 1080
Size: 103 977
SexAI A'CCwGG_T
Cuts at: 0 824 1080
Size: 824 256
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 793 1080
Size: 793 287
SpeI A'CTAG_T
Cuts at: 0 486 1080
Size: 486 594
SphI G_CATG'C
Cuts at: 0 266 1080
Size: 266 814
StuI AGG'CCT
Cuts at: 0 838 1080
Size: 838 242
StyI C'CwwG_G
Cuts at: 0 38 291 305 738 875 969 1080
Size: 38 253 14 433 137 94 111
Fragments arranged by size:
433 253 137 111 94 38 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3 404 1080
Size: 3 401 676
Fragments arranged by size:
676 401 3
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 942 974 1080
Size: 942 32 106
Fragments arranged by size:
942 106 32
Tth111I GACn'n_nGTC
Cuts at: 0 826 1080
Size: 826 254
XbaI T'CTAG_A
Cuts at: 0 1062 1080
Size: 1062 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 45 289 1080
Size: 45 244 791
Fragments arranged by size:
791 244 45
Enzymes that do cut:
AarI AcuI AhdI AlwNI ApaLI ApoI AvaI AvrII
BanI BbsI BcgI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BstEII BstYI BtgI BtgZI BtsI
DraIII EaeI EarI EciI Eco57MI EcoO109I FalI Hin4I
HincII MmeI MscI MslI MspA1I NcoI NspI PasI
PflMI PpuMI PvuII SexAI SfiI SpeI SphI StuI
StyI TaqII TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI AscI AseI AsiSI BaeI
BamHI BanII BbeI BbvCI BciVI BclI BfrBI BmgBI
BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BsgI BsiEI BsiWI BsmBI BspHI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SrfI SspI
SwaI TatI XhoI XmaI XmnI ZraI