Restriction map for
pFN21AB7147
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BcgI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsgI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
DraI DraIII Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRV EcoT14I EcoT22I
FseI FspI Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MseI MslI MunI NarI NcoI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI SacI SacII SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI Tru9I Tth111I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AccII 726 CGCG CG^CG
AccIII 518 TCCGGA T^CCGGA
AcsI 460 GAATTC [GA]^AATT[CT]
AflIII 725 ACGCGT A^C[GA][CT]GT
AlwNI 600 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 715 GGGCCC GGGCC^C
ApoI 460 GAATTC [GA]^AATT[CT]
AspHI 154 GTGCTC G[AT]GC[AT]^C
BalI 1014 TGGCCA TGG^CCA
BamHI 626 GGATCC G^GATCC
BanI 490 GGCACC G^G[CT][GA]CC
BbvCI 870 GCTGAGG CCTCAGC(-5/-2)
BciVI 3 GGATAC GTATCC(6/5)
BclI 590 TGATCA T^GATCA
BfaI 532 CTAG C^TAG
BglI 546 GCCCGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 778 TACGTG [CT]AC^GT[GA]
BsaWI 518 TCCGGA [AT]^CCGG[AT]
BseAI 518 TCCGGA T^CCGGA
BsiEI 376 CGGCCG CG[GA][CT]^CG
BsiHKAI 154 GTGCTC G[AT]GC[AT]^C
BspEI 518 TCCGGA T^CCGGA
BspHI 861 TCATGA T^CATGA
BsrBI 956 GAGCGG CCGCTC(-3/-3)
BsrFI 653 GCCGGC [GA]^CCGG[CT]
BstUI 726 CGCG CG^CG
CfoI 132 GCGC GCG^C
Cfr10I 653 GCCGGC [GA]^CCGG[CT]
CpoI 959 CGGACCG CG^G[AT]CCG
DrdI 814 GACCCCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 146 CCGTGG C^C[GA][CT]GG
EagI 376 CGGCCG C^GGCCG
EclXI 376 CGGCCG C^GGCCG
Eco52I 376 CGGCCG C^GGCCG
EcoRI 460 GAATTC G^AATTC
FbaI 590 TGATCA T^GATCA
HaeII 131 AGCGCC [GA]GCGC^[CT]
HhaI 132 GCGC GCG^C
HinP1I 132 GCGC G^CGC
HincII 811 GTCGAC GT[CT]^[GA]AC
HindII 811 GTCGAC GT[CT]^[GA]AC
HinfI 275 GATTC G^A[ACGT]TC
MaeI 532 CTAG C^TAG
MaeIII 781 GTGAC ^GT[ACGT]AC
MluI 725 ACGCGT A^CGCGT
MluNI 1014 TGGCCA TGG^CCA
MroI 518 TCCGGA T^CCGGA
MscI 1014 TGGCCA TGG^CCA
MvnI 726 CGCG CG^CG
NaeI 653 GCCGGC GCC^GGC
NgoMIV 653 GCCGGC G^CCGGC
NheI 531 GCTAGC G^CTAGC
NspI 233 GCATGC [GA]CATG^[CT]
PpuMI 499 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 861 TCATGA T^CATGA
RsrII 959 CGGACCG CG^G[AT]CCG
SalI 811 GTCGAC G^TCGAC
SapI 946 GAAGAGC GCTCTTC(1/4)
SphI 233 GCATGC GCATG^C
TaqI 812 TCGA T^CGA
TfiI 275 GATTC G^A[AT]TC
Tsp45I 781 GTGAC ^GT[GC]AC
Tsp509I 461 AATT ^AATT
TthHB8I 812 TCGA T^CGA
XcmI 486 CCAGGGCACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 60 GTAC GT^AC
366 GTAC
BbsI 49 GTCTTC GAAGAC(2/6)
112 GTCTTC
BpmI 511 CTGGAG CTGGAG(16/14)
556 CTGGAG
BpuAI 49 GTCTTC GAAGAC(2/6)
112 GTCTTC
BstF5I 255 CATCC GGATG(2/0)
537 CATCC
BstYI 626 GGATCC [GA]^GATC[CT]
773 GGATCT
EarI 310 CTCTTC CTCTTC(1/4)
946 GAAGAG
FauI 585 CCCGC CCCGC(4/6)
670 GCGGG
FokI 255 CATCC GGATG(9/13)
537 CATCC
HgaI 783 GACGC GACGC(5/10)
727 GCGTC
Ksp632I 310 CTCTTC CTCTTC(1/4)
946 GAAGAG
MflI 626 GGATCC [GA]^GATC[CT]
773 GGATCT
PflMI 265 CCACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
732 CCATGTCGTGG
PstI 127 CTGCAG CTGCA^G
438 CTGCAG
PvuII 320 CAGCTG CAG^CTG
968 CAGCTG
RsaI 60 GTAC GT^AC
366 GTAC
Van91I 265 CCACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
732 CCATGTCGTGG
XhoII 626 GGATCC [GA]^GATC[CT]
773 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccI AciI AluI AlwI AvaII
BanII BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BspMI BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII Fnu4HI HaeIII HapII
HpaII HphI ItaI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfcI TseI
TspRI