Restriction map for
pFN21AB7147
(Linear) (Six-base) MAPSORT of: KIBB7147.seq Check: 7838 from: 1 to: 1038
KIBB7147.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AccI GT'mk_AC
Cuts at: 0 449 812 824 1038
Size: 449 363 12 214
Fragments arranged by size:
449 363 214 12
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 946 1038
Size: 946 92
AflIII A'CryG_T
Cuts at: 0 725 1038
Size: 725 313
AlwNI CAG_nnn'CTG
Cuts at: 0 605 1038
Size: 605 433
ApaI G_GGCC'C
Cuts at: 0 719 1038
Size: 719 319
ApoI r'AATT_y
Cuts at: 0 460 1038
Size: 460 578
BamHI G'GATC_C
Cuts at: 0 626 1038
Size: 626 412
BanI G'GyrC_C
Cuts at: 0 490 1038
Size: 490 548
BanII G_rGCy'C
Cuts at: 0 86 719 890 1038
Size: 86 633 171 148
Fragments arranged by size:
633 171 148 86
BbsI GAAGACnn'nnnn_
Cuts at: 0 42 105 1038
Size: 42 63 933
Fragments arranged by size:
933 63 42
BbvCI CC'TCA_GC
Cuts at: 0 871 1038
Size: 871 167
BclI T'GATC_A
Cuts at: 0 590 1038
Size: 590 448
BglI GCCn_nnn'nGGC
Cuts at: 0 552 1038
Size: 552 486
Bme1580I G_kGCm'C
Cuts at: 0 493 719 1038
Size: 493 226 319
Fragments arranged by size:
493 319 226
BmgBI CAC'GTC
Cuts at: 0 153 1038
Size: 153 885
BmtI G_CTAG'C
Cuts at: 0 535 1038
Size: 535 503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 577 1038
Size: 532 45 461
Fragments arranged by size:
532 461 45
Bpu10I CC'TnA_GC
Cuts at: 0 871 1038
Size: 871 167
BsaAI yAC'GTr
Cuts at: 0 780 1038
Size: 780 258
BsaWI w'CCGG_w
Cuts at: 0 518 1038
Size: 518 520
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 817 820 847 850 867 897 1038
Size: 817 3 27 3 17 30 141
Fragments arranged by size:
817 141 30 27 17 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 675 825 828 888 1038
Size: 675 150 3 60 150
Fragments arranged by size:
675 150 150 60 3
BseYI C'CCAG_C
Cuts at: 0 384 1038
Size: 384 654
BsiEI CG_ry'CG
Cuts at: 0 379 1038
Size: 379 659
BsiHKAI G_wGCw'C
Cuts at: 0 158 1038
Size: 158 880
Bsp1286I G_dGCh'C
Cuts at: 0 86 158 493 719 890 1038
Size: 86 72 335 226 171 148
Fragments arranged by size:
335 226 171 148 86 72
BspEI T'CCGG_A
Cuts at: 0 518 1038
Size: 518 520
BspHI T'CATG_A
Cuts at: 0 861 1038
Size: 861 177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 104 191 385 511 1038
Size: 104 87 194 126 527
Fragments arranged by size:
527 194 126 104 87
BsrBI CCG'CTC
Cuts at: 0 958 1038
Size: 958 80
BsrFI r'CCGG_y
Cuts at: 0 653 1038
Size: 653 385
BstYI r'GATC_y
Cuts at: 0 626 773 1038
Size: 626 147 265
Fragments arranged by size:
626 265 147
BtgI C'CryG_G
Cuts at: 0 146 1038
Size: 146 892
BtsI GCAGTG_nn'
Cuts at: 0 435 1038
Size: 435 603
DrdI GACnn_nn'nnGTC
Cuts at: 0 820 1038
Size: 820 218
EaeI y'GGCC_r
Cuts at: 0 143 376 683 1014 1038
Size: 143 233 307 331 24
Fragments arranged by size:
331 307 233 143 24
EagI C'GGCC_G
Cuts at: 0 376 1038
Size: 376 662
EarI CTCTTCn'nnn_
Cuts at: 0 316 941 1038
Size: 316 625 97
Fragments arranged by size:
625 316 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 577 946 1038
Size: 532 45 369 92
Fragments arranged by size:
532 369 92 45
EcoO109I rG'GnC_Cy
Cuts at: 0 10 500 716 1038
Size: 10 490 216 322
Fragments arranged by size:
490 322 216 10
EcoRI G'AATT_C
Cuts at: 0 460 1038
Size: 460 578
HaeII r_GCGC'y
Cuts at: 0 135 1038
Size: 135 903
HincII GTy'rAC
Cuts at: 0 813 1038
Size: 813 225
MluI A'CGCG_T
Cuts at: 0 725 1038
Size: 725 313
MscI TGG'CCA
Cuts at: 0 1016 1038
Size: 1016 22
MspA1I CmG'CkG
Cuts at: 0 322 588 970 1038
Size: 322 266 382 68
Fragments arranged by size:
382 322 266 68
NaeI GCC'GGC
Cuts at: 0 655 1038
Size: 655 383
NgoMIV G'CCGG_C
Cuts at: 0 653 1038
Size: 653 385
NheI G'CTAG_C
Cuts at: 0 531 1038
Size: 531 507
NspI r_CATG'y
Cuts at: 0 237 1038
Size: 237 801
PflMI CCAn_nnn'nTGG
Cuts at: 0 271 738 1038
Size: 271 467 300
Fragments arranged by size:
467 300 271
PpuMI rG'GwC_Cy
Cuts at: 0 500 1038
Size: 500 538
PspOMI G'GGCC_C
Cuts at: 0 715 1038
Size: 715 323
PstI C_TGCA'G
Cuts at: 0 131 442 1038
Size: 131 311 596
Fragments arranged by size:
596 311 131
PvuII CAG'CTG
Cuts at: 0 322 970 1038
Size: 322 648 68
Fragments arranged by size:
648 322 68
RsrII CG'GwC_CG
Cuts at: 0 960 1038
Size: 960 78
SalI G'TCGA_C
Cuts at: 0 811 1038
Size: 811 227
SapI GCTCTTCn'nnn_
Cuts at: 0 941 1038
Size: 941 97
SfcI C'TryA_G
Cuts at: 0 127 438 609 1038
Size: 127 311 171 429
Fragments arranged by size:
429 311 171 127
SphI G_CATG'C
Cuts at: 0 237 1038
Size: 237 801
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 371 977 1038
Size: 371 606 61
Fragments arranged by size:
606 371 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 867 899 1038
Size: 867 32 139
Fragments arranged by size:
867 139 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 493 1038
Size: 493 545
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApoI BamHI BanI
BanII BbsI BbvCI BclI BglI Bme1580I BmgBI BmtI
BpmI Bpu10I BsaAI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BstYI
BtgI BtsI DrdI EaeI EagI EarI Eco57MI EcoO109I
EcoRI HaeII HincII MluI MscI MspA1I NaeI NgoMIV
NheI NspI PflMI PpuMI PspOMI PstI PvuII RsrII
SalI SapI SfcI SphI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BcgI BciVI BfrBI BglII BlpI
BmrI BplI BpuEI BsaI BsaBI BsaHI BsgI BsiWI
BsmI BsmBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EciI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI Hin4I HindIII HpaI KasI KpnI MfeI MmeI
MslI NarI NcoI NdeI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI SacI SacII SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI StuI StyI SwaI TatI Tth111I
XbaI XhoI XmaI XmnI ZraI