Restriction map for pFN21AB7144
 (Circular) (Six-base) MAPSORT of: pFN21AB7144.seq  Check: 8289  from: 1  to: 5722



pFN21AB7144  5722 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:26  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3532     278 
   Size:         53      83     186     936    1996    2468

  Fragments arranged by size: 

                2468    1996     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3065    1937 
   Size:       1128    4594
 
Acc65I G'GTAC_C

Cuts at:   3043    3043 
   Size:       5722
 
AclI AA'CG_TT

Cuts at:   1563    3849    4222    1563 
   Size:       2286     373    3063

  Fragments arranged by size: 

                3063    2286     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2360    2404    2916    3785    5169    5437    1632 
   Size:        728      44     512     869    1384     268    1917

  Fragments arranged by size: 

                1917    1384     869     728     512     268      44
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5525
 
AflIII A'CryG_T

Cuts at:   1203    2352    4621    5598    1203 
   Size:       1149    2269     977    1327

  Fragments arranged by size: 

                2269    1327    1149     977
 
AgeI A'CCGG_T

Cuts at:   4553    4553 
   Size:       5722
 
AhdI GACnn_n'nnGTC

Cuts at:   4451    4451 
   Size:       5722
 
AleI CACnn'nnGTG

Cuts at:   1166    2090    1166 
   Size:        924    4798
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2592    2908    1184 
   Size:       1408     316    3998

  Fragments arranged by size: 

                3998    1408     316
 
AlwNI CAG_nnn'CTG

Cuts at:   2433    2609    3465    5037    2433 
   Size:        176     856    1572    3118

  Fragments arranged by size: 

                3118    1572     856     176
 
ApaLI G'TGCA_C

Cuts at:   3779    4935    3779 
   Size:       1156    4566
 
ApoI r'AATT_y

Cuts at:   1493    2096    3031    3257    3409    5518    1493 
   Size:        603     935     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     935     603     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4276     160 
   Size:        872    3244    1606

  Fragments arranged by size: 

                3244    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5722
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3047    1870 
   Size:         73    1104    4545

  Fragments arranged by size: 

                4545    1104      73
 
BamHI G'GATC_C

Cuts at:   2905    3052    3419    2905 
   Size:        147     367    5208

  Fragments arranged by size: 

                5208     367     147
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3043    4498     618 
   Size:        325     217     627    1256    1455    1842

  Fragments arranged by size: 

                1842    1455    1256     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2317    3041    1391 
   Size:        926     724    4072

  Fragments arranged by size: 

                4072     926     724
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2141    2381    2749     928 
   Size:        971     242     240     368    3901

  Fragments arranged by size: 

                3901     971     368     242     240
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3913    3947    1903 
   Size:         34    1976      34    3678

  Fragments arranged by size: 

                3678    1976      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2555    3614    4824    2555 
   Size:       1059    1210    3453

  Fragments arranged by size: 

                3453    1210    1059
 
BclI T'GATC_A

Cuts at:   1548    2223    2637    1548 
   Size:        675     414    4633

  Fragments arranged by size: 

                4633     675     414
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4333     136 
   Size:        107     122      71     631     605    2661    1525

  Fragments arranged by size: 

                2661    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5717    5717 
   Size:       5722
 
BlpI GC'TnA_GC

Cuts at:   2610    3139    2610 
   Size:        529    5193
 
Bme1580I G_kGCm'C

Cuts at:   1790    3783    4939    1790 
   Size:       1993    1156    2573

  Fragments arranged by size: 

                2573    1993    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2379    1318 
   Size:       1061    4661
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2627    3453    4411 
   Size:        416      53      83     399      24    1210     826     958

Cuts at:   4411     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1210     958     826     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5722
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4382    1239 
   Size:       3143    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2396    2698    3847    4712    5010    2396 
   Size:        302    1149     865     298    3108

  Fragments arranged by size: 

                3108    1149     865     302     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4385     882 
   Size:        617    2886    2219

  Fragments arranged by size: 

                2886    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3513     493 
   Size:        711    2309    2702

  Fragments arranged by size: 

                2702    2309     711
 
BsaBI GATnn'nnATC

Cuts at:   2642    5716    2642 
   Size:       3074    2648
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3529    3911    4546     275 
   Size:         53      83     186     936    1996     382     635    1451

  Fragments arranged by size: 

                1996    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2271    4155    4553    4827    4974    5385    2271 
   Size:       1884     398     274     147     411    2608

  Fragments arranged by size: 

                2608    1884     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5497

  Fragments arranged by size: 

                5497     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2399    1186 
   Size:       1213    4509
 
BseYI C'CCAG_C

Cuts at:   4925    5421    4925 
   Size:        496    5226
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2184    1163 
   Size:        573     448    4701

  Fragments arranged by size: 

                4701     573     448
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3172    3933    4961    1540 
   Size:        275     186    1171     761    1028    2301

  Fragments arranged by size: 

                2301    1171    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3783    3868    4939    3783 
   Size:         85    1071    4566

  Fragments arranged by size: 

                4566    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2581    3233    3326    2581 
   Size:        652      93    4977

  Fragments arranged by size: 

                4977     652      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2317    3041    3783    3868    4939    1391 
   Size:        399     527     724     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     724     527     399      85
 
BspEI T'CCGG_A

Cuts at:   2271    5385    2271 
   Size:       3114    2608
 
BspHI T'CATG_A

Cuts at:   2220    2988    3611    5341    2220 
   Size:        768     623    1730    2601

  Fragments arranged by size: 

                2601    1730     768     623
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3077     844 
   Size:       2233    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2979    3609    1394 
   Size:        429    1156     630    3507

  Fragments arranged by size: 

                3507    1156     630     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4217    4391      65 
   Size:       1183    2969     174    1396

  Fragments arranged by size: 

                2969    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4366    4553    1700 
   Size:        110    2556     187    2869

  Fragments arranged by size: 

                2869    2556     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5722
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5722
 
BssSI C'ACGA_G

Cuts at:   3782    4794    3782 
   Size:       1012    4710
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5722
 
BstBI TT'CG_AA

Cuts at:   4526    4526 
   Size:       5722
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5722
 
BstYI r'GATC_y

Cuts at:   1972    2905    3052    3419    3804    3821    5273    5359    5371 
   Size:        933     147     367     385      17    1452      86      12

Cuts at:   5371    5717    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     933     385     367     346     147      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2805    2959     513 
   Size:        552     939     801     154    3276

  Fragments arranged by size: 

                3276     939     801     552     154
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2629     487 
   Size:        683      56    1403    3580

  Fragments arranged by size: 

                3580    1403     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2061    2726    3238    4032    4052    2061 
   Size:        665     512     794      20    3731

  Fragments arranged by size: 

                3731     794     665     512      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5722
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2852    2887    2983    3018     529 
   Size:         35    2288      35      96      35    3233

  Fragments arranged by size: 

                3233    2288      96      35      35      35
 
DraI TTT'AAA

Cuts at:   3026    3026 
   Size:       5722
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4729     809 
   Size:        507    3413    1802

  Fragments arranged by size: 

                3413    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3169    4058    5687 
   Size:         54    1425      50     275      18    1339     889    1629

Cuts at:   5687       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1339     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3169    1537 
   Size:        275    1357    4090

  Fragments arranged by size: 

                4090    1357     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3652    1353 
   Size:       2299    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4309    4683    4829     387 
   Size:       1064     138    2720     374     146    1280

  Fragments arranged by size: 

                2720    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2360    2404    2916    3785    4382    5169    5437 
   Size:        393     728      44     512     869     597     787     268

Cuts at:   5437    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     869     787     728     597     512     393     268
                  44
 
EcoRI G'AATT_C

Cuts at:   3031    3409    3031 
   Size:        378    5344
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2563    2563 
   Size:       5722
 
FspI TGC'GCA

Cuts at:   4228    4228 
   Size:       5722
 
HaeII r_GCGC'y

Cuts at:   1678    4869    1678 
   Size:       3191    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2613    2645    2936    2968    4363 
   Size:         32     135      32    1041      32     291      32    1395

Cuts at:   4363    4395    4437    4469    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1395    1041     291     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3066    3312     669 
   Size:        988     281    1128     246    3079

  Fragments arranged by size: 

                3079    1128     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5722
 
HpaI GTT'AAC

Cuts at:   3312    3312 
   Size:       5722
 
KpnI G_GTAC'C

Cuts at:   3047    3047 
   Size:       5722
 
MfeI C'AATT_G

Cuts at:   3321    3321 
   Size:       5722
 
MluI A'CGCG_T

Cuts at:   5598    5598 
   Size:       5722
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4836    5020    1305 
   Size:       3531     184    2007

  Fragments arranged by size: 

                3531    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3900

  Fragments arranged by size: 

                3900    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2090    2219    3680    4039    4198     518 
   Size:        648     924     129    1461     359     159    2042

  Fragments arranged by size: 

                2042    1461     924     648     359     159     129
 
MspA1I CmG'CkG

Cuts at:   1606    3138    3815    4963    5208    1606 
   Size:       1532     677    1148     245    2120

  Fragments arranged by size: 

                2120    1532    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2805    2959     513 
   Size:        552     939     801     154    3276

  Fragments arranged by size: 

                3276     939     801     552     154
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5722
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5722
 
NotI GC'GGCC_GC

Cuts at:   3169    3169 
   Size:       5722
 
NspI r_CATG'y

Cuts at:   1132    1229    2356    3080    4625    1132 
   Size:         97    1127     724    1545    2229

  Fragments arranged by size: 

                2229    1545    1127     724      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5722
 
PciI A'CATG_T

Cuts at:   2352    4621    2352 
   Size:       2269    3453
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2433    1108 
   Size:       1325    4397
 
PmeI GTTT'AAAC

Cuts at:   3026    3026 
   Size:       5722
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3791    3823    5304    5336    3791 
   Size:         32    1481      32    4177

  Fragments arranged by size: 

                4177    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5722
 
PsiI TTA'TAA

Cuts at:   3292    3292 
   Size:       5722
 
PstI C_TGCA'G

Cuts at:    830    2755    3074     830 
   Size:       1925     319    3478

  Fragments arranged by size: 

                3478    1925     319
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5722
 
SalI G'TCGA_C

Cuts at:   1936    3064    1936 
   Size:       1128    4594
 
SbfI CC_TGCA'GG

Cuts at:   3074    3074 
   Size:       5722
 
ScaI AGT'ACT

Cuts at:   3970    5682    3970 
   Size:       1712    4010
 
SfcI C'TryA_G

Cuts at:    826    1045    2751    3070    4205    4886    5077     826 
   Size:        219    1706     319    1135     681     191    1471

  Fragments arranged by size: 

                1706    1471    1135     681     319     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5722
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5722
 
SmaI CCC'GGG

Cuts at:   1872    3049    1872 
   Size:       1177    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2411    2677    3826    4727    4989     820 
   Size:        197     926     468     266    1149     901     262    1553

  Fragments arranged by size: 

                1553    1149     926     901     468     266     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    3513     493 
   Size:       3020    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5722
 
SphI G_CATG'C

Cuts at:   1132    3080    1132 
   Size:       1948    3774
 
SspI AAT'ATT

Cuts at:      5      52    3646       5 
   Size:         47    3594    2081

  Fragments arranged by size: 

                3594    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5722
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2701    2805    2959    3161     513 
   Size:        552     939     697     104     154     202    3074

  Fragments arranged by size: 

                3074     939     697     552     202     154     104
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3749    3766    3919    4104    4549     325 
   Size:       1255    2169      17     153     185     445    1498

  Fragments arranged by size: 

                2169    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3968    5680      96 
   Size:        274      80      33      51    1443    1991    1712     138

  Fragments arranged by size: 

                1991    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2844    2876    5682    5714    2844 
   Size:         32    2806      32    2852

  Fragments arranged by size: 

                2852    2806      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5722
 
XbaI T'CTAG_A

Cuts at:   3058    3058 
   Size:       5722
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5722
 
XmaI C'CCGG_G

Cuts at:   1870    3047    1870 
   Size:       1177    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3851    3851 
   Size:       5722
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3530     276 
   Size:         53      83     186     936    1996    2468

  Fragments arranged by size: 

                2468    1996     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI     PstI     PvuI     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
     XcmI
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