(Circular) (Six-base) MAPSORT of: pFN21AB7144.seq Check: 8289 from: 1 to: 5722 pFN21AB7144 5722 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3532 278 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3065 1937 Size: 1128 4594 Acc65I G'GTAC_C Cuts at: 3043 3043 Size: 5722 AclI AA'CG_TT Cuts at: 1563 3849 4222 1563 Size: 2286 373 3063 Fragments arranged by size: 3063 2286 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2360 2404 2916 3785 5169 5437 1632 Size: 728 44 512 869 1384 268 1917 Fragments arranged by size: 1917 1384 869 728 512 268 44 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5525 AflIII A'CryG_T Cuts at: 1203 2352 4621 5598 1203 Size: 1149 2269 977 1327 Fragments arranged by size: 2269 1327 1149 977 AgeI A'CCGG_T Cuts at: 4553 4553 Size: 5722 AhdI GACnn_n'nnGTC Cuts at: 4451 4451 Size: 5722 AleI CACnn'nnGTG Cuts at: 1166 2090 1166 Size: 924 4798 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2592 2908 1184 Size: 1408 316 3998 Fragments arranged by size: 3998 1408 316 AlwNI CAG_nnn'CTG Cuts at: 2433 2609 3465 5037 2433 Size: 176 856 1572 3118 Fragments arranged by size: 3118 1572 856 176 ApaLI G'TGCA_C Cuts at: 3779 4935 3779 Size: 1156 4566 ApoI r'AATT_y Cuts at: 1493 2096 3031 3257 3409 5518 1493 Size: 603 935 226 152 2109 1697 Fragments arranged by size: 2109 1697 935 603 226 152 AseI AT'TA_AT Cuts at: 160 1032 4276 160 Size: 872 3244 1606 Fragments arranged by size: 3244 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 AvaI C'yCGr_G Cuts at: 1870 1943 3047 1870 Size: 73 1104 4545 Fragments arranged by size: 4545 1104 73 BamHI G'GATC_C Cuts at: 2905 3052 3419 2905 Size: 147 367 5208 Fragments arranged by size: 5208 367 147 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3043 4498 618 Size: 325 217 627 1256 1455 1842 Fragments arranged by size: 1842 1455 1256 627 325 217 BanII G_rGCy'C Cuts at: 1391 2317 3041 1391 Size: 926 724 4072 Fragments arranged by size: 4072 926 724 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2141 2381 2749 928 Size: 971 242 240 368 3901 Fragments arranged by size: 3901 971 368 242 240 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3913 3947 1903 Size: 34 1976 34 3678 Fragments arranged by size: 3678 1976 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2555 3614 4824 2555 Size: 1059 1210 3453 Fragments arranged by size: 3453 1210 1059 BclI T'GATC_A Cuts at: 1548 2223 2637 1548 Size: 675 414 4633 Fragments arranged by size: 4633 675 414 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4333 136 Size: 107 122 71 631 605 2661 1525 Fragments arranged by size: 2661 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5717 5717 Size: 5722 BlpI GC'TnA_GC Cuts at: 2610 3139 2610 Size: 529 5193 Bme1580I G_kGCm'C Cuts at: 1790 3783 4939 1790 Size: 1993 1156 2573 Fragments arranged by size: 2573 1993 1156 BmgBI CAC'GTC Cuts at: 1318 2379 1318 Size: 1061 4661 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2627 3453 4411 Size: 416 53 83 399 24 1210 826 958 Cuts at: 4411 442 Size: 1753 Fragments arranged by size: 1753 1210 958 826 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5722 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4382 1239 Size: 3143 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2396 2698 3847 4712 5010 2396 Size: 302 1149 865 298 3108 Fragments arranged by size: 3108 1149 865 302 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4385 882 Size: 617 2886 2219 Fragments arranged by size: 2886 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3513 493 Size: 711 2309 2702 Fragments arranged by size: 2702 2309 711 BsaBI GATnn'nnATC Cuts at: 2642 5716 2642 Size: 3074 2648 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3529 3911 4546 275 Size: 53 83 186 936 1996 382 635 1451 Fragments arranged by size: 1996 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2271 4155 4553 4827 4974 5385 2271 Size: 1884 398 274 147 411 2608 Fragments arranged by size: 2608 1884 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5497 Fragments arranged by size: 5497 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2399 1186 Size: 1213 4509 BseYI C'CCAG_C Cuts at: 4925 5421 4925 Size: 496 5226 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2184 1163 Size: 573 448 4701 Fragments arranged by size: 4701 573 448 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3172 3933 4961 1540 Size: 275 186 1171 761 1028 2301 Fragments arranged by size: 2301 1171 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3783 3868 4939 3783 Size: 85 1071 4566 Fragments arranged by size: 4566 1071 85 BsmI GAATG_Cn' Cuts at: 2581 3233 3326 2581 Size: 652 93 4977 Fragments arranged by size: 4977 652 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2317 3041 3783 3868 4939 1391 Size: 399 527 724 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 724 527 399 85 BspEI T'CCGG_A Cuts at: 2271 5385 2271 Size: 3114 2608 BspHI T'CATG_A Cuts at: 2220 2988 3611 5341 2220 Size: 768 623 1730 2601 Fragments arranged by size: 2601 1730 768 623 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3077 844 Size: 2233 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2979 3609 1394 Size: 429 1156 630 3507 Fragments arranged by size: 3507 1156 630 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4217 4391 65 Size: 1183 2969 174 1396 Fragments arranged by size: 2969 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4366 4553 1700 Size: 110 2556 187 2869 Fragments arranged by size: 2869 2556 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5722 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5722 BssSI C'ACGA_G Cuts at: 3782 4794 3782 Size: 1012 4710 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5722 BstBI TT'CG_AA Cuts at: 4526 4526 Size: 5722 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5722 BstYI r'GATC_y Cuts at: 1972 2905 3052 3419 3804 3821 5273 5359 5371 Size: 933 147 367 385 17 1452 86 12 Cuts at: 5371 5717 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 933 385 367 346 147 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2805 2959 513 Size: 552 939 801 154 3276 Fragments arranged by size: 3276 939 801 552 154 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2629 487 Size: 683 56 1403 3580 Fragments arranged by size: 3580 1403 683 56 BtsI GCAGTG_nn' Cuts at: 2061 2726 3238 4032 4052 2061 Size: 665 512 794 20 3731 Fragments arranged by size: 3731 794 665 512 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5722 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2852 2887 2983 3018 529 Size: 35 2288 35 96 35 3233 Fragments arranged by size: 3233 2288 96 35 35 35 DraI TTT'AAA Cuts at: 3026 3026 Size: 5722 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4729 809 Size: 507 3413 1802 Fragments arranged by size: 3413 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3169 4058 5687 Size: 54 1425 50 275 18 1339 889 1629 Cuts at: 5687 8 Size: 43 Fragments arranged by size: 1629 1425 1339 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3169 1537 Size: 275 1357 4090 Fragments arranged by size: 4090 1357 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3652 1353 Size: 2299 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4309 4683 4829 387 Size: 1064 138 2720 374 146 1280 Fragments arranged by size: 2720 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2360 2404 2916 3785 4382 5169 5437 Size: 393 728 44 512 869 597 787 268 Cuts at: 5437 1239 Size: 1524 Fragments arranged by size: 1524 869 787 728 597 512 393 268 44 EcoRI G'AATT_C Cuts at: 3031 3409 3031 Size: 378 5344 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2563 2563 Size: 5722 FspI TGC'GCA Cuts at: 4228 4228 Size: 5722 HaeII r_GCGC'y Cuts at: 1678 4869 1678 Size: 3191 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2613 2645 2936 2968 4363 Size: 32 135 32 1041 32 291 32 1395 Cuts at: 4363 4395 4437 4469 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1395 1041 291 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3066 3312 669 Size: 988 281 1128 246 3079 Fragments arranged by size: 3079 1128 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5722 HpaI GTT'AAC Cuts at: 3312 3312 Size: 5722 KpnI G_GTAC'C Cuts at: 3047 3047 Size: 5722 MfeI C'AATT_G Cuts at: 3321 3321 Size: 5722 MluI A'CGCG_T Cuts at: 5598 5598 Size: 5722 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4836 5020 1305 Size: 3531 184 2007 Fragments arranged by size: 3531 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3900 Fragments arranged by size: 3900 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2090 2219 3680 4039 4198 518 Size: 648 924 129 1461 359 159 2042 Fragments arranged by size: 2042 1461 924 648 359 159 129 MspA1I CmG'CkG Cuts at: 1606 3138 3815 4963 5208 1606 Size: 1532 677 1148 245 2120 Fragments arranged by size: 2120 1532 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2805 2959 513 Size: 552 939 801 154 3276 Fragments arranged by size: 3276 939 801 552 154 NdeI CA'TA_TG Cuts at: 387 387 Size: 5722 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5722 NotI GC'GGCC_GC Cuts at: 3169 3169 Size: 5722 NspI r_CATG'y Cuts at: 1132 1229 2356 3080 4625 1132 Size: 97 1127 724 1545 2229 Fragments arranged by size: 2229 1545 1127 724 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5722 PciI A'CATG_T Cuts at: 2352 4621 2352 Size: 2269 3453 PflMI CCAn_nnn'nTGG Cuts at: 1108 2433 1108 Size: 1325 4397 PmeI GTTT'AAAC Cuts at: 3026 3026 Size: 5722 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3791 3823 5304 5336 3791 Size: 32 1481 32 4177 Fragments arranged by size: 4177 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5722 PsiI TTA'TAA Cuts at: 3292 3292 Size: 5722 PstI C_TGCA'G Cuts at: 830 2755 3074 830 Size: 1925 319 3478 Fragments arranged by size: 3478 1925 319 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5722 SalI G'TCGA_C Cuts at: 1936 3064 1936 Size: 1128 4594 SbfI CC_TGCA'GG Cuts at: 3074 3074 Size: 5722 ScaI AGT'ACT Cuts at: 3970 5682 3970 Size: 1712 4010 SfcI C'TryA_G Cuts at: 826 1045 2751 3070 4205 4886 5077 826 Size: 219 1706 319 1135 681 191 1471 Fragments arranged by size: 1706 1471 1135 681 319 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5722 SmaI CCC'GGG Cuts at: 1872 3049 1872 Size: 1177 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2411 2677 3826 4727 4989 820 Size: 197 926 468 266 1149 901 262 1553 Fragments arranged by size: 1553 1149 926 901 468 266 262 197 SnaBI TAC'GTA Cuts at: 493 3513 493 Size: 3020 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5722 SphI G_CATG'C Cuts at: 1132 3080 1132 Size: 1948 3774 SspI AAT'ATT Cuts at: 5 52 3646 5 Size: 47 3594 2081 Fragments arranged by size: 3594 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5722 StyI C'CwwG_G Cuts at: 513 1065 2004 2701 2805 2959 3161 513 Size: 552 939 697 104 154 202 3074 Fragments arranged by size: 3074 939 697 552 202 154 104 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3749 3766 3919 4104 4549 325 Size: 1255 2169 17 153 185 445 1498 Fragments arranged by size: 2169 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3968 5680 96 Size: 274 80 33 51 1443 1991 1712 138 Fragments arranged by size: 1991 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2844 2876 5682 5714 2844 Size: 32 2806 32 2852 Fragments arranged by size: 2852 2806 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5722 XbaI T'CTAG_A Cuts at: 3058 3058 Size: 5722 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5722 XmaI C'CCGG_G Cuts at: 1870 3047 1870 Size: 1177 4545 XmnI GAAnn'nnTTC Cuts at: 3851 3851 Size: 5722 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3530 276 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI