(Circular) (Six-base) MAPSORT of: pFN21AB7140.seq Check: 8494 from: 1 to: 5704 pFN21AB7140 5704 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3514 278 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2809 3047 1937 Size: 872 238 4594 Fragments arranged by size: 4594 872 238 Acc65I G'GTAC_C Cuts at: 3025 3025 Size: 5704 AclI AA'CG_TT Cuts at: 1563 3831 4204 1563 Size: 2268 373 3063 Fragments arranged by size: 3063 2268 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3767 5151 5419 1632 Size: 2135 1384 268 1917 Fragments arranged by size: 2135 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5507 AflIII A'CryG_T Cuts at: 1203 4603 5580 1203 Size: 3400 977 1327 Fragments arranged by size: 3400 1327 977 AgeI A'CCGG_T Cuts at: 4535 4535 Size: 5704 AhdI GACnn_n'nnGTC Cuts at: 4433 4433 Size: 5704 AleI CACnn'nnGTG Cuts at: 1166 2015 2198 1166 Size: 849 183 4672 Fragments arranged by size: 4672 849 183 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5704 AlwNI CAG_nnn'CTG Cuts at: 3447 5019 3447 Size: 1572 4132 ApaLI G'TGCA_C Cuts at: 3761 4917 3761 Size: 1156 4548 ApoI r'AATT_y Cuts at: 1493 2909 3013 3239 3391 5500 1493 Size: 1416 104 226 152 2109 1697 Fragments arranged by size: 2109 1697 1416 226 152 104 AseI AT'TA_AT Cuts at: 160 1032 4258 160 Size: 872 3226 1606 Fragments arranged by size: 3226 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 AvaI C'yCGr_G Cuts at: 1870 1943 3029 1870 Size: 73 1086 4545 Fragments arranged by size: 4545 1086 73 BamHI G'GATC_C Cuts at: 2878 3034 3401 2878 Size: 156 367 5181 Fragments arranged by size: 5181 367 156 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2046 2260 3025 4480 618 Size: 325 217 627 259 214 765 1455 1842 Fragments arranged by size: 1842 1455 765 627 325 259 217 214 BanII G_rGCy'C Cuts at: 1391 2349 3023 1391 Size: 958 674 4072 Fragments arranged by size: 4072 958 674 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2722 928 Size: 971 823 3910 Fragments arranged by size: 3910 971 823 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2487 2521 3895 3929 1903 Size: 34 550 34 1374 34 3678 Fragments arranged by size: 3678 1374 550 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3596 4806 3596 Size: 1210 4494 BclI T'GATC_A Cuts at: 1548 1548 Size: 5704 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2545 4315 136 Size: 107 122 71 631 605 873 1770 1525 Fragments arranged by size: 1770 1525 873 631 605 122 107 71 BglII A'GATC_T Cuts at: 2036 5699 2036 Size: 3663 2041 BlpI GC'TnA_GC Cuts at: 3121 3121 Size: 5704 Bme1580I G_kGCm'C Cuts at: 1790 2051 3765 4921 1790 Size: 261 1714 1156 2573 Fragments arranged by size: 2573 1714 1156 261 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5704 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3435 4393 442 Size: 416 53 83 399 24 2018 958 1753 Fragments arranged by size: 2018 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5704 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4364 1239 Size: 3125 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2354 2503 3829 4694 4992 2354 Size: 149 1326 865 298 3066 Fragments arranged by size: 3066 1326 865 298 149 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2309 2434 4367 882 Size: 617 810 125 1933 2219 Fragments arranged by size: 2219 1933 810 617 125 BsaAI yAC'GTr Cuts at: 493 1204 3495 493 Size: 711 2291 2702 Fragments arranged by size: 2702 2291 711 BsaBI GATnn'nnATC Cuts at: 5698 5698 Size: 5704 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3511 3893 4528 275 Size: 53 83 186 936 1978 382 635 1451 Fragments arranged by size: 1978 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4137 4535 4809 4956 5367 4137 Size: 398 274 147 411 4474 Fragments arranged by size: 4474 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5479 Fragments arranged by size: 5479 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2155 1186 Size: 969 4735 BseYI C'CCAG_C Cuts at: 2083 2159 4907 5403 2083 Size: 76 2748 496 2384 Fragments arranged by size: 2748 2384 496 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5131 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3154 3915 4943 1540 Size: 275 186 1153 761 1028 2301 Fragments arranged by size: 2301 1153 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3765 3850 4921 3765 Size: 85 1071 4548 Fragments arranged by size: 4548 1071 85 BsmI GAATG_Cn' Cuts at: 3215 3308 3215 Size: 93 5611 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2349 3023 3765 3850 4921 1391 Size: 399 261 298 674 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 674 399 298 261 85 BspEI T'CCGG_A Cuts at: 5367 5367 Size: 5704 BspHI T'CATG_A Cuts at: 3593 5323 3593 Size: 1730 3974 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3059 844 Size: 2215 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2044 3591 1394 Size: 429 221 1547 3507 Fragments arranged by size: 3507 1547 429 221 BsrDI GCAATG_nn' Cuts at: 65 1248 4199 4373 65 Size: 1183 2951 174 1396 Fragments arranged by size: 2951 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2111 4348 4535 1700 Size: 110 301 2237 187 2869 Fragments arranged by size: 2869 2237 301 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5704 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5704 BssSI C'ACGA_G Cuts at: 3764 4776 3764 Size: 1012 4692 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5704 BstBI TT'CG_AA Cuts at: 4508 4508 Size: 5704 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5704 BstXI CCAn_nnnn'nTGG Cuts at: 2326 2326 Size: 5704 BstYI r'GATC_y Cuts at: 1972 2036 2878 3034 3401 3786 3803 5255 5341 Size: 64 842 156 367 385 17 1452 86 Cuts at: 5341 5353 5699 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 842 385 367 346 156 86 64 17 12 Bsu36I CC'TnA_GG Cuts at: 2998 2998 Size: 5704 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5152 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4965 Fragments arranged by size: 4965 683 56 BtsI GCAGTG_nn' Cuts at: 3220 4014 4034 3220 Size: 794 20 4890 Fragments arranged by size: 4890 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5704 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5669 DraI TTT'AAA Cuts at: 3008 3008 Size: 5704 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4711 809 Size: 507 3395 1802 Fragments arranged by size: 3395 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2202 3151 4040 Size: 54 1425 50 275 18 372 949 889 Cuts at: 4040 5669 8 Size: 1629 43 Fragments arranged by size: 1629 1425 949 889 372 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3151 1537 Size: 275 1339 4090 Fragments arranged by size: 4090 1339 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3634 1353 Size: 2281 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4291 4665 4811 387 Size: 1064 138 2702 374 146 1280 Fragments arranged by size: 2702 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3767 4364 5151 5419 1239 Size: 393 2135 597 787 268 1524 Fragments arranged by size: 2135 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2537 2537 Size: 5704 EcoRI G'AATT_C Cuts at: 3013 3391 3013 Size: 378 5326 FspI TGC'GCA Cuts at: 4210 4210 Size: 5704 HaeII r_GCGC'y Cuts at: 1678 4851 1678 Size: 3173 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4345 4377 4419 4451 1373 Size: 32 135 32 2773 32 42 32 2626 Fragments arranged by size: 2773 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3048 3294 669 Size: 988 281 1110 246 3079 Fragments arranged by size: 3079 1110 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5704 HpaI GTT'AAC Cuts at: 3294 3294 Size: 5704 KpnI G_GTAC'C Cuts at: 3029 3029 Size: 5704 MfeI C'AATT_G Cuts at: 3303 3303 Size: 5704 MluI A'CGCG_T Cuts at: 5580 5580 Size: 5704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4818 5002 1305 Size: 3513 184 2007 Fragments arranged by size: 3513 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3882 Fragments arranged by size: 3882 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2015 2018 2198 2324 2873 3662 4021 Size: 648 849 3 180 126 549 789 359 Cuts at: 4021 4180 518 Size: 159 2042 Fragments arranged by size: 2042 849 789 648 549 359 180 159 126 3 MspA1I CmG'CkG Cuts at: 1606 2074 3120 3797 4945 5190 1606 Size: 468 1046 677 1148 245 2120 Fragments arranged by size: 2120 1148 1046 677 468 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5152 NdeI CA'TA_TG Cuts at: 387 2576 387 Size: 2189 3515 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5704 NotI GC'GGCC_GC Cuts at: 3151 3151 Size: 5704 NspI r_CATG'y Cuts at: 1132 1229 3062 4607 1132 Size: 97 1833 1545 2229 Fragments arranged by size: 2229 1833 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5704 PciI A'CATG_T Cuts at: 4603 4603 Size: 5704 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5704 PmeI GTTT'AAAC Cuts at: 3008 3008 Size: 5704 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3773 3805 5286 5318 3773 Size: 32 1481 32 4159 Fragments arranged by size: 4159 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5704 PsiI TTA'TAA Cuts at: 3274 3274 Size: 5704 PstI C_TGCA'G Cuts at: 830 3056 830 Size: 2226 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5704 PvuII CAG'CTG Cuts at: 2074 2074 Size: 5704 SalI G'TCGA_C Cuts at: 1936 3046 1936 Size: 1110 4594 SbfI CC_TGCA'GG Cuts at: 3056 3056 Size: 5704 ScaI AGT'ACT Cuts at: 3952 5664 3952 Size: 1712 3992 SfcI C'TryA_G Cuts at: 826 1045 2806 3052 4187 4868 5059 826 Size: 219 1761 246 1135 681 191 1471 Fragments arranged by size: 1761 1471 1135 681 246 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5704 SmaI CCC'GGG Cuts at: 1872 3031 1872 Size: 1159 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2369 2482 3808 4709 4971 820 Size: 197 926 426 113 1326 901 262 1553 Fragments arranged by size: 1553 1326 926 901 426 262 197 113 SnaBI TAC'GTA Cuts at: 493 3495 493 Size: 3002 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5704 SphI G_CATG'C Cuts at: 1132 3062 1132 Size: 1930 3774 SspI AAT'ATT Cuts at: 5 52 2395 3628 5 Size: 47 2343 1233 2081 Fragments arranged by size: 2343 2081 1233 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5704 StyI C'CwwG_G Cuts at: 513 1065 3143 513 Size: 552 2078 3074 Fragments arranged by size: 3074 2078 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2131 3731 3748 3901 4086 4531 325 Size: 1255 551 1600 17 153 185 445 1498 Fragments arranged by size: 1600 1498 1255 551 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2068 3950 5662 Size: 274 80 33 51 1443 91 1882 1712 Cuts at: 5662 96 Size: 138 Fragments arranged by size: 1882 1712 1443 274 138 91 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5664 5696 5664 Size: 32 5672 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5704 XbaI T'CTAG_A Cuts at: 3040 3040 Size: 5704 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5704 XmaI C'CCGG_G Cuts at: 1870 3029 1870 Size: 1159 4545 XmnI GAAnn'nnTTC Cuts at: 3833 3833 Size: 5704 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3512 276 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI