Restriction map for
pFN21AB7137
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AhdI
Alw44I AlwI AlwNI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BseAI BsiEI BsiWI BsmI BsoBI
BspDI BspEI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstYI BstZ17I CelII ClaI CpoI
DpnII DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HpaI KasI KpnI KspI MaeI
MboI MfeI MflI MluI MroI
MseI MunI NaeI NarI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI Sau3AI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 699 CGCG CG^CG
AgeI 416 ACCGGT A^CCGGT
AocI 954 CCTCAGG CC^T[ACGT]AGG
ApaI 164 GGGCCC GGGCC^C
AvaII 594 GGTCC G^G[AT]CC
BbrPI 366 CACGTG CAC^GTG
BpmI 720 CTCCAG CTGGAG(16/14)
BsaAI 366 CACGTG [CT]AC^GT[GA]
BsaBI 690 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 840 GGTCTC GGTCTC(1/5)
BsaWI 416 ACCGGT [AT]^CCGG[AT]
BseRI 394 CTCCTC GAGGAG(10/8)
BsgI 270 CTGCAC GTGCAG(16/14)
BsmBI 717 CGTCTC CGTCTC(1/5)
Bsp1407I 884 TGTACA T^GTACA
BspHI 644 TCATGA T^CATGA
BspMI 772 ACCTGC ACCTGC(4/8)
BsrFI 416 ACCGGT [GA]^CCGG[CT]
BsrGI 884 TGTACA T^GTACA
BsrI 302 CCAGT ACTGG(1/-1)
BstEII 78 GGTAACC G^GT[ACGT]ACC
BstPI 78 GGTAACC G^GT[ACGT]ACC
BstUI 699 CGCG CG^CG
BstXI 290 CCAAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 954 CCTCAGG CC^T[ACGT]AGG
Cfr10I 416 ACCGGT [GA]^CCGG[CT]
DrdI 693 GACCATCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 791 CCGTGG C^C[GA][CT]GG
Eco81I 954 CCTCAGG CC^T[ACGT]AGG
EcoNI 746 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 78 GGTAACC G^GT[ACGT]ACC
HaeII 294 GGCGCT [GA]GCGC^[CT]
HgaI 700 GCGTC GACGC(5/10)
HindIII 961 AAGCTT A^AGCTT
MamI 690 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 699 CGCG CG^CG
NspI 800 GCATGT [GA]CATG^[CT]
PflMI 69 CCAGACGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 416 ACCGGT A^CCGGT
PmaCI 366 CACGTG CAC^GTG
PmlI 366 CACGTG CAC^GTG
PpuMI 593 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 138 CAGCTG CAG^CTG
RcaI 644 TCATGA T^CATGA
SacI 321 GAGCTC GAGCT^C
SbfI 93 CCTGCAGG CCTGCA^GG
Sse8387I 93 CCTGCAGG CCTGCA^GG
SspBI 884 TGTACA T^GTACA
Tsp45I 20 GTCAC ^GT[GC]AC
TspRI 888 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 69 CCAGACGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Asp700I 759 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
960 GAAGCTTTTC
AspHI 109 GAGCAC G[AT]GC[AT]^C
321 GAGCTC
BbsI 738 GAAGAC GAAGAC(2/6)
817 GTCTTC
BcnI 123 CCGGG CC^[GC]GG
837 CCCGG
BpuAI 738 GAAGAC GAAGAC(2/6)
817 GTCTTC
BsiHKAI 109 GAGCAC G[AT]GC[AT]^C
321 GAGCTC
BsmAI 718 GTCTC GTCTC(1/5)
841 GTCTC
FauI 438 CCCGC CCCGC(4/6)
479 GCGGG
HincII 514 GTCAAC GT[CT]^[GA]AC
634 GTCAAC
HindII 514 GTCAAC GT[CT]^[GA]AC
634 GTCAAC
HinfI 18 GAGTC G^A[ACGT]TC
897 GAGTC
NciI 123 CCGGG CC^[GC]GG
837 CCCGG
PleI 18 GAGTC GAGTC(4/5)
897 GAGTC
SapI 861 GAAGAGC GCTCTTC(1/4)
776 GCTCTTC
XmnI 759 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
960 GAAGCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI BalI BanII
BbvI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeII MaeIII MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NlaIII NlaIV PstI RsaI
Sau96I ScrFI SfaNI SfcI StyI
TseI