(Circular) (Six-base) MAPSORT of: pFN21AB7137.seq Check: 2229 from: 1 to: 5689 pFN21AB7137 5689 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2786 2786 Size: 5689 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3032 1937 Size: 1095 4594 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2756 2965 3752 5136 5404 1632 Size: 1124 209 787 1384 268 1917 Fragments arranged by size: 1917 1384 1124 787 268 209 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 4588 5565 1203 Size: 3385 977 1327 Fragments arranged by size: 3385 1327 977 AgeI A'CCGG_T Cuts at: 2421 4520 2421 Size: 2099 3590 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 2498 2798 1166 Size: 1332 300 4057 Fragments arranged by size: 4057 1332 300 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2452 1184 Size: 1268 4421 AlwNI CAG_nnn'CTG Cuts at: 3432 5004 3432 Size: 1572 4117 ApaI G_GGCC'C Cuts at: 2173 2173 Size: 5689 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2998 3224 3376 5485 1493 Size: 1505 226 152 2109 1697 Fragments arranged by size: 2109 1697 1505 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 3014 1870 Size: 73 1071 4545 Fragments arranged by size: 4545 1071 73 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3010 4465 618 Size: 325 217 627 1223 1455 1842 Fragments arranged by size: 1842 1455 1223 627 325 217 BanII G_rGCy'C Cuts at: 1391 2173 2223 2330 3008 1391 Size: 782 50 107 678 4072 Fragments arranged by size: 4072 782 678 107 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2750 2815 928 Size: 971 851 65 3802 Fragments arranged by size: 3802 971 851 65 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3880 3914 1903 Size: 34 1943 34 3678 Fragments arranged by size: 3678 1943 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4300 136 Size: 107 122 71 631 605 2628 1525 Fragments arranged by size: 2628 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 3106 3106 Size: 5689 Bme1580I G_kGCm'C Cuts at: 1790 2173 3750 4906 1790 Size: 383 1577 1156 2573 Fragments arranged by size: 2573 1577 1156 383 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3420 4378 442 Size: 416 53 83 399 24 2003 958 1753 Fragments arranged by size: 2003 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2925 2925 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2710 4349 1239 Size: 1471 1639 2579 Fragments arranged by size: 2579 1639 1471 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3814 4679 4977 3814 Size: 865 298 4526 Fragments arranged by size: 4526 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2851 4352 882 Size: 617 1352 1501 2219 Fragments arranged by size: 2219 1501 1352 617 BsaAI yAC'GTr Cuts at: 493 1204 2373 3480 493 Size: 711 1169 1107 2702 Fragments arranged by size: 2702 1169 1107 711 BsaBI GATnn'nnATC Cuts at: 2699 5683 2699 Size: 2984 2705 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3496 3878 4513 275 Size: 53 83 186 936 1963 382 635 1451 Fragments arranged by size: 1963 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2421 4122 4520 4794 4941 5352 2421 Size: 1701 398 274 147 411 2758 Fragments arranged by size: 2758 1701 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5464 Fragments arranged by size: 5464 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2390 1186 Size: 1204 4485 BseYI C'CCAG_C Cuts at: 2051 2355 4892 5388 2051 Size: 304 2537 496 2352 Fragments arranged by size: 2537 2352 496 304 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2260 1163 Size: 573 524 4592 Fragments arranged by size: 4592 573 524 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 2330 3750 3835 4906 2118 Size: 212 1420 85 1071 2901 Fragments arranged by size: 2901 1420 1071 212 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 BsmBI CGTCTCn'nnnn_ Cuts at: 2728 2728 Size: 5689 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2173 2223 2330 3008 3750 3835 Size: 399 328 55 50 107 678 742 85 Cuts at: 3835 4906 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 678 399 328 107 85 55 50 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 2649 3578 5308 2649 Size: 929 1730 3030 Fragments arranged by size: 3030 1730 929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2786 3044 844 Size: 1942 258 3489 Fragments arranged by size: 3489 1942 258 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2421 4333 4520 1700 Size: 110 611 1912 187 2869 Fragments arranged by size: 2869 1912 611 187 110 BsrGI T'GTAC_A Cuts at: 96 2889 96 Size: 2793 2896 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 3749 4761 3749 Size: 1012 4677 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5689 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 2083 1184 Size: 899 4790 BstXI CCAn_nnnn'nTGG Cuts at: 2302 2302 Size: 5689 BstYI r'GATC_y Cuts at: 1972 3019 3386 3771 3788 5240 5326 5338 5684 Size: 1047 367 385 17 1452 86 12 346 Cuts at: 5684 1972 Size: 1977 Fragments arranged by size: 1977 1452 1047 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2960 2960 Size: 5689 BtgI C'CryG_G Cuts at: 513 1065 2796 513 Size: 552 1731 3406 Fragments arranged by size: 3406 1731 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2686 487 Size: 683 56 1460 3490 Fragments arranged by size: 3490 1460 683 56 BtsI GCAGTG_nn' Cuts at: 3205 3999 4019 3205 Size: 794 20 4875 Fragments arranged by size: 4875 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2704 4696 809 Size: 507 1388 1992 1802 Fragments arranged by size: 1992 1802 1388 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2304 2490 Size: 54 1425 50 275 18 369 105 186 Cuts at: 2490 2526 3136 4025 5654 8 Size: 36 610 889 1629 43 Fragments arranged by size: 1629 1425 889 610 369 275 186 105 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2159 2287 2316 2788 2861 3619 1353 Size: 806 128 29 472 73 758 3423 Fragments arranged by size: 3423 806 758 472 128 73 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2678 4276 4650 4796 387 Size: 1064 138 1089 1598 374 146 1280 Fragments arranged by size: 1598 1280 1089 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2328 2328 Size: 5689 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2710 2756 2965 3752 4349 5136 5404 Size: 393 1078 46 209 787 597 787 268 Cuts at: 5404 1239 Size: 1524 Fragments arranged by size: 1524 1078 787 787 597 393 268 209 46 EcoNI CCTnn'n_nnAGG Cuts at: 2755 2755 Size: 5689 EcoO109I rG'GnC_Cy Cuts at: 2169 2170 2599 2169 Size: 1 429 5259 Fragments arranged by size: 5259 429 1 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 2303 4836 1678 Size: 625 2533 2531 Fragments arranged by size: 2533 2531 625 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2008 2040 4330 4362 4404 Size: 32 135 32 436 32 2290 32 42 Cuts at: 4404 4436 1373 Size: 32 2626 Fragments arranged by size: 2626 2290 436 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2521 2641 3033 3279 669 Size: 988 281 583 120 392 246 3079 Fragments arranged by size: 3079 988 583 392 281 246 120 HindIII A'AGCT_T Cuts at: 748 2966 748 Size: 2218 3471 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2298 4803 4987 1305 Size: 993 2505 184 2007 Fragments arranged by size: 2505 2007 993 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2201 2306 2492 10 Size: 54 1425 343 369 105 186 3207 Fragments arranged by size: 3207 1425 369 343 186 105 54 MslI CAynn'nnrTG Cuts at: 518 1166 2408 2498 2798 3647 4006 4165 518 Size: 648 1242 90 300 849 359 159 2042 Fragments arranged by size: 2042 1242 849 648 359 300 159 90 MspA1I CmG'CkG Cuts at: 1606 2145 2446 2484 2819 3105 3782 4930 5175 Size: 539 301 38 335 286 677 1148 245 Cuts at: 5175 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 539 335 301 286 245 38 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5137 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 2809 3047 4592 1132 Size: 97 1580 238 1545 2229 Fragments arranged by size: 2229 1580 1545 238 97 PasI CC'CwG_GG Cuts at: 1348 2216 1348 Size: 868 4821 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 2080 1108 Size: 972 4717 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PmlI CAC'GTG Cuts at: 2373 2373 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2599 2599 Size: 5689 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PspOMI G'GGCC_C Cuts at: 2169 2169 Size: 5689 PstI C_TGCA'G Cuts at: 830 2103 2483 2627 3041 830 Size: 1273 380 144 414 3478 Fragments arranged by size: 3478 1273 414 380 144 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 PvuII CAG'CTG Cuts at: 2145 2145 Size: 5689 SacI G_AGCT'C Cuts at: 2330 2330 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SapI GCTCTTCn'nnn_ Cuts at: 2788 2861 2788 Size: 73 5616 SbfI CC_TGCA'GG Cuts at: 2103 3041 2103 Size: 938 4751 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SfcI C'TryA_G Cuts at: 826 1045 2099 2479 2623 2854 3037 4172 4853 Size: 219 1054 380 144 231 183 1135 681 Cuts at: 4853 5044 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1054 681 380 231 219 191 183 144 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 3016 1872 Size: 1144 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3793 4694 4956 820 Size: 197 926 1850 901 262 1553 Fragments arranged by size: 1850 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5689 StyI C'CwwG_G Cuts at: 513 1065 2057 2196 2295 3128 513 Size: 552 992 139 99 833 3074 Fragments arranged by size: 3074 992 833 552 139 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2133 3716 3733 3886 4071 4516 325 Size: 1255 553 1583 17 153 185 445 1498 Fragments arranged by size: 1583 1498 1255 553 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2235 2889 3935 Size: 274 80 33 51 1443 258 654 1046 Cuts at: 3935 5647 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1046 654 274 258 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5649 5681 5649 Size: 32 5657 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 3014 1870 Size: 1144 4545 XmnI GAAnn'nnTTC Cuts at: 2768 2969 3818 2768 Size: 201 849 4639 Fragments arranged by size: 4639 849 201 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI