Restriction map for
pFN21AB7137
(Linear) (Six-base) MAPSORT of: KIBB7137.seq Check: 7763 from: 1 to: 984
KIBB7137.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 781 984
Size: 781 203
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 960 984
Size: 751 209 24
Fragments arranged by size:
751 209 24
AgeI A'CCGG_T
Cuts at: 0 416 984
Size: 416 568
AleI CACnn'nnGTG
Cuts at: 0 493 793 984
Size: 493 300 191
Fragments arranged by size:
493 300 191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 447 984
Size: 447 537
ApaI G_GGCC'C
Cuts at: 0 168 984
Size: 168 816
BanII G_rGCy'C
Cuts at: 0 168 218 325 984
Size: 168 50 107 659
Fragments arranged by size:
659 168 107 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 745 810 984
Size: 745 65 174
Fragments arranged by size:
745 174 65
Bme1580I G_kGCm'C
Cuts at: 0 168 984
Size: 168 816
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 920 984
Size: 920 64
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 984
Size: 705 279
BsaI GGTCTCn'nnnn_
Cuts at: 0 846 984
Size: 846 138
BsaAI yAC'GTr
Cuts at: 0 368 984
Size: 368 616
BsaBI GATnn'nnATC
Cuts at: 0 694 984
Size: 694 290
BsaWI w'CCGG_w
Cuts at: 0 416 984
Size: 416 568
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 984
Size: 385 599
BseYI C'CCAG_C
Cuts at: 0 46 350 984
Size: 46 304 634
Fragments arranged by size:
634 304 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 984
Size: 255 729
BsiHKAI G_wGCw'C
Cuts at: 0 113 325 984
Size: 113 212 659
Fragments arranged by size:
659 212 113
BsmBI CGTCTCn'nnnn_
Cuts at: 0 723 984
Size: 723 261
Bsp1286I G_dGCh'C
Cuts at: 0 113 168 218 325 984
Size: 113 55 50 107 659
Fragments arranged by size:
659 113 107 55 50
BspHI T'CATG_A
Cuts at: 0 644 984
Size: 644 340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 781 984
Size: 781 203
BsrFI r'CCGG_y
Cuts at: 0 416 984
Size: 416 568
BsrGI T'GTAC_A
Cuts at: 0 884 984
Size: 884 100
BstEII G'GTnAC_C
Cuts at: 0 78 984
Size: 78 906
BstXI CCAn_nnnn'nTGG
Cuts at: 0 297 984
Size: 297 687
Bsu36I CC'TnA_GG
Cuts at: 0 955 984
Size: 955 29
BtgI C'CryG_G
Cuts at: 0 791 984
Size: 791 193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 681 984
Size: 681 303
DrdI GACnn_nn'nnGTC
Cuts at: 0 699 984
Size: 699 285
EaeI y'GGCC_r
Cuts at: 0 194 299 485 521 984
Size: 194 105 186 36 463
Fragments arranged by size:
463 194 186 105 36
EarI CTCTTCn'nnn_
Cuts at: 0 154 282 311 783 856 984
Size: 154 128 29 472 73 128
Fragments arranged by size:
472 154 128 128 73 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 673 984
Size: 673 311
EcoICRI GAG'CTC
Cuts at: 0 323 984
Size: 323 661
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 751 960 984
Size: 705 46 209 24
Fragments arranged by size:
705 209 46 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 750 984
Size: 750 234
EcoO109I rG'GnC_Cy
Cuts at: 0 164 165 594 984
Size: 164 1 429 390
Fragments arranged by size:
429 390 164 1
HaeII r_GCGC'y
Cuts at: 0 298 984
Size: 298 686
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 984
Size: 3 32 949
Fragments arranged by size:
949 32 3
HincII GTy'rAC
Cuts at: 0 516 636 984
Size: 516 120 348
Fragments arranged by size:
516 348 120
HindIII A'AGCT_T
Cuts at: 0 961 984
Size: 961 23
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 984
Size: 293 691
MscI TGG'CCA
Cuts at: 0 196 301 487 984
Size: 196 105 186 497
Fragments arranged by size:
497 196 186 105
MslI CAynn'nnrTG
Cuts at: 0 403 493 793 984
Size: 403 90 300 191
Fragments arranged by size:
403 300 191 90
MspA1I CmG'CkG
Cuts at: 0 140 441 479 814 984
Size: 140 301 38 335 170
Fragments arranged by size:
335 301 170 140 38
NspI r_CATG'y
Cuts at: 0 804 984
Size: 804 180
PasI CC'CwG_GG
Cuts at: 0 211 984
Size: 211 773
PflMI CCAn_nnn'nTGG
Cuts at: 0 75 984
Size: 75 909
PmlI CAC'GTG
Cuts at: 0 368 984
Size: 368 616
PpuMI rG'GwC_Cy
Cuts at: 0 594 984
Size: 594 390
PspOMI G'GGCC_C
Cuts at: 0 164 984
Size: 164 820
PstI C_TGCA'G
Cuts at: 0 98 478 622 984
Size: 98 380 144 362
Fragments arranged by size:
380 362 144 98
PvuII CAG'CTG
Cuts at: 0 140 984
Size: 140 844
SacI G_AGCT'C
Cuts at: 0 325 984
Size: 325 659
SapI GCTCTTCn'nnn_
Cuts at: 0 783 856 984
Size: 783 73 128
Fragments arranged by size:
783 128 73
SbfI CC_TGCA'GG
Cuts at: 0 98 984
Size: 98 886
SfcI C'TryA_G
Cuts at: 0 94 474 618 849 984
Size: 94 380 144 231 135
Fragments arranged by size:
380 231 144 135 94
StyI C'CwwG_G
Cuts at: 0 52 191 290 984
Size: 52 139 99 694
Fragments arranged by size:
694 139 99 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 128 984
Size: 128 856
TatI w'GTAC_w
Cuts at: 0 230 884 984
Size: 230 654 100
Fragments arranged by size:
654 230 100
XmnI GAAnn'nnTTC
Cuts at: 0 763 964 984
Size: 763 201 20
Fragments arranged by size:
763 201 20
Enzymes that do cut:
AarI AcuI AgeI AleI AlfI ApaI BanII BbsI
Bme1580I BplI BpmI BsaI BsaAI BsaBI BsaWI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrFI
BsrGI BstEII BstXI Bsu36I BtgI BtgZI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII HindIII MmeI MscI MslI MspA1I NspI PasI
PflMI PmlI PpuMI PspOMI PstI PvuII SacI SapI
SbfI SfcI StyI TaqII TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
Bpu10I BpuEI BsaHI BsaXI BsiEI BsiWI BsmI BspEI
BsrBI BsrDI BssHII BssSI BstAPI BstBI BstYI BstZ17I
BtsI ClaI CspCI DraI DraIII EagI EcoRI EcoRV
FalI FseI FspI FspAI HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI