Restriction map for
pFN21AB7135
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HapII
HgaI Hin1I HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MroI MslI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
TaqI Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcsI 31 GAATTC [GA]^AATT[CT]
AflIII 718 ACATGT A^C[GA][CT]GT
Alw44I 247 GTGCAC G^TGCAC
ApaLI 247 GTGCAC G^TGCAC
ApoI 31 GAATTC [GA]^AATT[CT]
Asp700I 569 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 247 GTGCAC G[AT]GC[AT]^C
BalI 734 TGGCCA TGG^CCA
BamHI 680 GGATCC G^GATCC
BfaI 187 CTAG C^TAG
BmyI 247 GTGCAC G[AGT]GC[ACT]^C
BseRI 253 CTCCTC GAGGAG(10/8)
BsiHKAI 247 GTGCAC G[AT]GC[AT]^C
BsmAI 335 GTCTC GTCTC(1/5)
BsmFI 232 GTCCC GGGAC(10/14)
Bsp1286I 247 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 830 TGTACA T^GTACA
BspLU11I 718 ACATGT A^CATGT
BspMI 161 ACCTGC ACCTGC(4/8)
BsrGI 830 TGTACA T^GTACA
BssKI 732 CCTGG ^CC[ACGT]GG
BstF5I 540 CATCC GGATG(2/0)
BstNI 732 CCTGG CC^[AT]GG
BstYI 680 GGATCC [GA]^GATC[CT]
Cac8I 494 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 761 GCGC GCG^C
Cfr13I 782 GGCCC G^G[ACGT]CC
EaeI 734 TGGCCA [CT]^GGCC[GA]
EcoRI 31 GAATTC G^AATTC
EcoRII 732 CCTGG ^CC[AT]GG
FokI 540 CATCC GGATG(9/13)
HaeII 760 AGCGCC [GA]GCGC^[CT]
HhaI 761 GCGC GCG^C
HinP1I 761 GCGC G^CGC
HincII 483 GTTGAC GT[CT]^[GA]AC
HindII 483 GTTGAC GT[CT]^[GA]AC
HphI 83 TCACC GGTGA(8/7)
MaeI 187 CTAG C^TAG
MflI 680 GGATCC [GA]^GATC[CT]
MluNI 734 TGGCCA TGG^CCA
MscI 734 TGGCCA TGG^CCA
MvaI 732 CCTGG CC^[AT]GG
MwoI 346 GCAGCAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 680 GGATCC GG[ACGT]^[ACGT]CC
NspI 718 ACATGT [GA]CATG^[CT]
PflMI 237 CCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 624 GACTC GAGTC(4/5)
Sau96I 782 GGCCC G^G[ACGT]CC
ScrFI 732 CCTGG CC^[ACGT]GG
SfcI 908 CTATAG C^T[GA][CT]AG
SmlI 675 CTTGAG C^T[CT][GA]AG
SspBI 830 TGTACA T^GTACA
Van91I 237 CCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 680 GGATCC [GA]^GATC[CT]
XmnI 569 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 9 enzymes) #
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AluI 21 AGCT AG^CT
897 AGCT
AseI 129 ATTAAT AT^TAAT
520 ATTAAT
AsnI 129 ATTAAT AT^TAAT
520 ATTAAT
BbsI 698 GAAGAC GAAGAC(2/6)
610 GTCTTC
BpuAI 698 GAAGAC GAAGAC(2/6)
610 GTCTTC
NdeI 356 CATATG CA^TATG
773 CATATG
PshBI 129 ATTAAT AT^TAAT
520 ATTAAT
TfiI 12 GAATC G^A[AT]TC
47 GATTC
Tsp45I 148 GTGAC ^GT[GC]AC
536 GTGAC
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AfaI AlwI BbvI BsiYI BslI
BsrDI BsrI DdeI DpnII Fnu4HI
HaeIII HinfI ItaI MaeIII MboI
MboII MnlI MseI NdeII NlaIII
RsaI Sau3AI Tru9I TseI Tsp509I
TspRI