Restriction map for
pFN21AB7118
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
CpoI DpnII DraI DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI MaeI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SalI
SapI Sau3AI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 342 CGCG CG^CG
AcyI 70 GACGCC G[GA]^CG[CT]C
AhdI 662 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 662 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 705 GTGCTC G[AT]GC[AT]^C
BsaBI 324 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 70 GACGCC G[GA]^CG[CT]C
BsaI 764 GGTCTC GGTCTC(1/5)
BsgI 566 CTGCAC GTGCAG(16/14)
BsiHKAI 705 GTGCTC G[AT]GC[AT]^C
BspDI 195 ATCGAT AT^CGAT
BsrBI 75 CCGCTC CCGCTC(-3/-3)
BsrFI 736 GCCGGT [GA]^CCGG[CT]
BstUI 342 CGCG CG^CG
Cfr10I 736 GCCGGT [GA]^CCGG[CT]
ClaI 195 ATCGAT AT^CGAT
DsaI 341 CCGCGG C^C[GA][CT]GG
Eam1105I 662 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 619 GAAGAG CTCTTC(1/4)
EcoT14I 747 CCAAGG C^C[AT][AT]GG
HgaI 70 GACGC GACGC(5/10)
Hin1I 70 GACGCC G[GA]^CG[CT]C
HinfI 499 GATTC G^A[ACGT]TC
Ksp632I 619 GAAGAG CTCTTC(1/4)
KspI 341 CCGCGG CCGC^GG
MaeIII 422 GTGAC ^GT[ACGT]AC
MamI 324 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 341 CCGCGG C[AC]G^C[GT]G
MvnI 342 CGCG CG^CG
NlaIII 664 CATG CATG^
SacII 341 CCGCGG CCGC^GG
SfaNI 285 GATGC GCATC(5/9)
SmaI 440 CCCGGG CCC^GGG
StuI 368 AGGCCT AGG^CCT
StyI 747 CCAAGG C^C[AT][AT]GG
TaqI 196 TCGA T^CGA
TfiI 499 GATTC G^A[AT]TC
Tsp45I 422 GTGAC ^GT[GC]AC
TthHB8I 196 TCGA T^CGA
XmaCI 440 CCCGGG C^CCGGG
XmaI 440 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 279 GTAC GT^AC
408 GTAC
AluI 512 AGCT AG^CT
755 AGCT
BbvCI 183 CCTCAGC CCTCAGC(-5/-2)
371 CCTCAGC
BpmI 514 CTCCAG CTGGAG(16/14)
103 CTGGAG
BsaAI 280 TACGTG [CT]AC^GT[GA]
409 TACGTG
BsmAI 679 GAGAC GTCTC(1/5)
765 GTCTC
CfoI 611 GCGC GCG^C
691 GCGC
DrdI 646 GACCAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
794 GACCAGTGGGTC
EaeI 530 TGGCCG [CT]^GGCC[GA]
734 TGGCCG
HhaI 611 GCGC GCG^C
691 GCGC
HinP1I 611 GCGC G^CGC
691 GCGC
MslI 192 CACATCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
838 CACCCCAGTG
RsaI 279 GTAC GT^AC
408 GTAC
SmlI 547 CTCAAG C^T[CT][GA]AG
718 CTTGAG
XcmI 239 CCACCAACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
560 CCAGGGCTGCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI ApaI AvaI AvaII BanII
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MaeII
MboII MnlI MspI MvaI MwoI
NciI NlaIV PpuMI Sau96I ScrFI
TseI TspRI