(Circular) (Six-base) MAPSORT of: pFN21AB7118.seq Check: 955 from: 1 to: 5554 pFN21AB7118 5554 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3364 278 Size: 53 83 186 936 1828 2468 Fragments arranged by size: 2468 1828 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2897 1937 Size: 960 4594 Acc65I G'GTAC_C Cuts at: 2875 2875 Size: 5554 AclI AA'CG_TT Cuts at: 1563 3681 4054 1563 Size: 2118 373 3063 Fragments arranged by size: 3063 2118 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2114 2437 3617 5001 5269 1632 Size: 482 323 1180 1384 268 1917 Fragments arranged by size: 1917 1384 1180 482 323 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5357 AflIII A'CryG_T Cuts at: 1203 4453 5430 1203 Size: 3250 977 1327 Fragments arranged by size: 3250 1327 977 AgeI A'CCGG_T Cuts at: 4385 4385 Size: 5554 AhdI GACnn_n'nnGTC Cuts at: 2672 4283 2672 Size: 1611 3943 AleI CACnn'nnGTG Cuts at: 1166 2847 1166 Size: 1681 3873 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5554 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2540 2572 2540 Size: 32 5522 AlwNI CAG_nnn'CTG Cuts at: 3297 4869 3297 Size: 1572 3982 ApaI G_GGCC'C Cuts at: 2211 2234 2444 2211 Size: 23 210 5321 Fragments arranged by size: 5321 210 23 ApaLI G'TGCA_C Cuts at: 3611 4767 3611 Size: 1156 4398 ApoI r'AATT_y Cuts at: 1493 2863 3089 3241 5350 1493 Size: 1370 226 152 2109 1697 Fragments arranged by size: 2109 1697 1370 226 152 AseI AT'TA_AT Cuts at: 160 1032 4108 160 Size: 872 3076 1606 Fragments arranged by size: 3076 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 AvaI C'yCGr_G Cuts at: 1870 1943 2158 2445 2489 2879 1870 Size: 73 215 287 44 390 4545 Fragments arranged by size: 4545 390 287 215 73 44 BamHI G'GATC_C Cuts at: 2884 3251 2884 Size: 367 5187 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2875 4330 618 Size: 325 217 627 1088 1455 1842 Fragments arranged by size: 1842 1455 1088 627 325 217 BanII G_rGCy'C Cuts at: 1391 2144 2211 2234 2312 2444 2476 2873 1391 Size: 753 67 23 78 132 32 397 4072 Fragments arranged by size: 4072 753 397 132 78 67 32 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4583 BbvCI CC'TCA_GC Cuts at: 2189 2377 2189 Size: 188 5366 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3745 3779 1903 Size: 34 1808 34 3678 Fragments arranged by size: 3678 1808 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3446 4656 3446 Size: 1210 4344 BclI T'GATC_A Cuts at: 1548 1548 Size: 5554 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4165 136 Size: 107 122 71 631 605 2493 1525 Fragments arranged by size: 2493 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5549 5549 Size: 5554 BlpI GC'TnA_GC Cuts at: 2971 2971 Size: 5554 Bme1580I G_kGCm'C Cuts at: 1790 2091 2211 2234 2444 3615 4771 1790 Size: 301 120 23 210 1171 1156 2573 Fragments arranged by size: 2573 1171 1156 301 210 120 23 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5554 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2585 2672 2841 Size: 416 53 83 399 24 1168 87 169 Cuts at: 2841 3285 4243 442 Size: 444 958 1753 Fragments arranged by size: 1753 1168 958 444 416 399 169 87 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5554 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2728 2728 Size: 5554 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2504 4214 1239 Size: 890 375 1710 2579 Fragments arranged by size: 2579 1710 890 375 Bpu10I CC'TnA_GC Cuts at: 2189 2377 2713 2189 Size: 188 336 5030 Fragments arranged by size: 5030 336 188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2537 2744 3679 4544 4842 2537 Size: 207 935 865 298 3249 Fragments arranged by size: 3249 935 865 298 207 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2775 4217 882 Size: 617 1276 1442 2219 Fragments arranged by size: 2219 1442 1276 617 BsaAI yAC'GTr Cuts at: 493 1204 2287 2416 3345 493 Size: 711 1083 129 929 2702 Fragments arranged by size: 2702 1083 929 711 129 BsaBI GATnn'nnATC Cuts at: 2333 5548 2333 Size: 3215 2339 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2076 3361 3743 4378 Size: 53 83 186 936 543 1285 382 635 Cuts at: 4378 275 Size: 1451 Fragments arranged by size: 1451 1285 936 635 543 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3987 4385 4659 4806 5217 3987 Size: 398 274 147 411 4324 Fragments arranged by size: 4324 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2693 2723 1178 Size: 30 165 30 1290 30 4009 Fragments arranged by size: 4009 1290 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2021 2143 2642 2717 1186 Size: 835 122 499 75 4023 Fragments arranged by size: 4023 835 499 122 75 BseYI C'CCAG_C Cuts at: 2024 2211 4757 5253 2024 Size: 187 2546 496 2325 Fragments arranged by size: 2546 2325 496 187 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2556 1163 Size: 573 820 4161 Fragments arranged by size: 4161 820 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3004 3765 4793 1540 Size: 275 186 1003 761 1028 2301 Fragments arranged by size: 2301 1028 1003 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2714 3615 3700 4771 2714 Size: 901 85 1071 3497 Fragments arranged by size: 3497 1071 901 85 BsmI GAATG_Cn' Cuts at: 3065 3158 3065 Size: 93 5461 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2144 2211 2234 2312 2444 2476 Size: 399 301 53 67 23 78 132 32 Cuts at: 2476 2714 2873 3615 3700 4771 1391 Size: 238 159 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 301 238 159 132 85 78 67 53 32 23 BspEI T'CCGG_A Cuts at: 5217 5217 Size: 5554 BspHI T'CATG_A Cuts at: 3443 5173 3443 Size: 1730 3824 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2909 844 Size: 2065 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2082 3441 1394 Size: 429 259 1359 3507 Fragments arranged by size: 3507 1359 429 259 BsrDI GCAATG_nn' Cuts at: 65 1248 4049 4223 65 Size: 1183 2801 174 1396 Fragments arranged by size: 2801 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2741 4198 4385 1700 Size: 110 931 1457 187 2869 Fragments arranged by size: 2869 1457 931 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5554 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5554 BssSI C'ACGA_G Cuts at: 3614 4626 3614 Size: 1012 4542 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5554 BstBI TT'CG_AA Cuts at: 4358 4358 Size: 5554 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5554 BstXI CCAn_nnnn'nTGG Cuts at: 2107 2203 2224 2254 2602 2669 2107 Size: 96 21 30 348 67 4992 Fragments arranged by size: 4992 348 96 67 30 21 BstYI r'GATC_y Cuts at: 1972 2884 3251 3636 3653 5105 5191 5203 5549 Size: 912 367 385 17 1452 86 12 346 Cuts at: 5549 1972 Size: 1977 Fragments arranged by size: 1977 1452 912 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2346 513 Size: 552 939 342 3721 Fragments arranged by size: 3721 939 552 342 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4815 Fragments arranged by size: 4815 683 56 BtsI GCAGTG_nn' Cuts at: 2842 3070 3864 3884 2842 Size: 228 794 20 4512 Fragments arranged by size: 4512 794 228 20 ClaI AT'CG_AT Cuts at: 1554 2201 1554 Size: 647 4907 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5519 DraI TTT'AAA Cuts at: 2858 2858 Size: 5554 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2657 2805 4561 809 Size: 507 1341 148 1756 1802 Fragments arranged by size: 1802 1756 1341 507 148 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2535 2739 3001 Size: 54 1425 50 275 18 705 204 262 Cuts at: 3001 3890 5519 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 705 275 262 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3001 1537 Size: 275 1189 4090 Fragments arranged by size: 4090 1189 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2619 3484 1353 Size: 1266 865 3423 Fragments arranged by size: 3423 1266 865 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2267 4141 4515 4661 387 Size: 1064 138 678 1874 374 146 1280 Fragments arranged by size: 1874 1280 1064 678 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2129 2437 2504 3617 4214 5001 Size: 393 482 15 308 67 1113 597 787 Cuts at: 5001 5269 1239 Size: 268 1524 Fragments arranged by size: 1524 1113 787 597 482 393 308 268 67 15 EcoO109I rG'GnC_Cy Cuts at: 2207 2208 2230 2440 2441 2461 2674 2719 2807 Size: 1 22 210 1 20 213 45 88 Cuts at: 2807 2207 Size: 4954 Fragments arranged by size: 4954 213 210 88 45 22 20 1 1 EcoRI G'AATT_C Cuts at: 2863 3241 2863 Size: 378 5176 FspI TGC'GCA Cuts at: 4060 4060 Size: 5554 HaeII r_GCGC'y Cuts at: 1678 4701 1678 Size: 3023 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2066 2098 2129 2161 2658 Size: 32 135 32 494 32 31 32 497 Cuts at: 2658 2690 4195 4227 4269 4301 1373 Size: 32 1505 32 42 32 2626 Fragments arranged by size: 2626 1505 497 494 135 42 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 2898 3144 669 Size: 988 281 960 246 3079 Fragments arranged by size: 3079 988 960 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5554 HpaI GTT'AAC Cuts at: 3144 3144 Size: 5554 KpnI G_GTAC'C Cuts at: 2879 2879 Size: 5554 MfeI C'AATT_G Cuts at: 3153 3153 Size: 5554 MluI A'CGCG_T Cuts at: 5430 5430 Size: 5554 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4668 4852 1305 Size: 3363 184 2007 Fragments arranged by size: 3363 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3732 Fragments arranged by size: 3732 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 2847 3512 3871 4030 518 Size: 648 1035 646 665 359 159 2042 Fragments arranged by size: 2042 1035 665 648 646 359 159 MspA1I CmG'CkG Cuts at: 1606 2348 2970 3647 4795 5040 1606 Size: 742 622 677 1148 245 2120 Fragments arranged by size: 2120 1148 742 677 622 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4063 Fragments arranged by size: 4063 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5554 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5554 NotI GC'GGCC_GC Cuts at: 3001 3001 Size: 5554 NspI r_CATG'y Cuts at: 1132 1229 2912 4457 1132 Size: 97 1683 1545 2229 Fragments arranged by size: 2229 1683 1545 97 PasI CC'CwG_GG Cuts at: 1348 2224 2794 1348 Size: 876 570 4108 Fragments arranged by size: 4108 876 570 PciI A'CATG_T Cuts at: 4453 4453 Size: 5554 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5554 PfoI T'CCnGG_A Cuts at: 2106 2253 2106 Size: 147 5407 PmeI GTTT'AAAC Cuts at: 2858 2858 Size: 5554 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3623 3655 5136 5168 3623 Size: 32 1481 32 4009 Fragments arranged by size: 4009 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2461 2674 2719 2807 2461 Size: 213 45 88 5208 Fragments arranged by size: 5208 213 88 45 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5554 PsiI TTA'TAA Cuts at: 3124 3124 Size: 5554 PspOMI G'GGCC_C Cuts at: 2207 2230 2440 2207 Size: 23 210 5321 Fragments arranged by size: 5321 210 23 PstI C_TGCA'G Cuts at: 830 2906 830 Size: 2076 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5554 SacII CC_GC'GG Cuts at: 2349 2349 Size: 5554 SalI G'TCGA_C Cuts at: 1936 2896 1936 Size: 960 4594 SanDI GG'GwC_CC Cuts at: 2674 2807 2674 Size: 133 5421 SbfI CC_TGCA'GG Cuts at: 2906 2906 Size: 5554 ScaI AGT'ACT Cuts at: 3802 5514 3802 Size: 1712 3842 SfcI C'TryA_G Cuts at: 826 1045 2902 4037 4718 4909 826 Size: 219 1857 1135 681 191 1471 Fragments arranged by size: 1857 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5554 SmaI CCC'GGG Cuts at: 1872 2447 2881 1872 Size: 575 434 4545 Fragments arranged by size: 4545 575 434 SmlI C'TyrA_G Cuts at: 820 1017 1943 2552 2723 3658 4559 4821 820 Size: 197 926 609 171 935 901 262 1553 Fragments arranged by size: 1553 935 926 901 609 262 197 171 SnaBI TAC'GTA Cuts at: 493 3345 493 Size: 2852 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5554 SphI G_CATG'C Cuts at: 1132 2912 1132 Size: 1780 3774 SspI AAT'ATT Cuts at: 5 52 3478 5 Size: 47 3426 2081 Fragments arranged by size: 3426 2081 47 StuI AGG'CCT Cuts at: 1517 2375 1517 Size: 858 4696 StyI C'CwwG_G Cuts at: 513 1065 2004 2752 2993 513 Size: 552 939 748 241 3074 Fragments arranged by size: 3074 939 748 552 241 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2558 3581 3598 3751 3936 4381 325 Size: 1255 978 1023 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1023 978 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3800 5512 96 Size: 274 80 33 51 1443 1823 1712 138 Fragments arranged by size: 1823 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2693 2725 5514 5546 2693 Size: 32 2789 32 2701 Fragments arranged by size: 2789 2701 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5554 XbaI T'CTAG_A Cuts at: 2890 2890 Size: 5554 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2251 2572 2251 Size: 321 5233 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5554 XmaI C'CCGG_G Cuts at: 1870 2445 2879 1870 Size: 575 434 4545 Fragments arranged by size: 4545 575 434 XmnI GAAnn'nnTTC Cuts at: 3683 3683 Size: 5554 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3362 276 Size: 53 83 186 936 1828 2468 Fragments arranged by size: 2468 1828 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI