Restriction map for
pFN21AB7118
(Linear) (Six-base) MAPSORT of: KIBB7118.seq Check: 6397 from: 1 to: 849
KIBB7118.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 432 849
Size: 109 323 417
Fragments arranged by size:
417 323 109
AhdI GACnn_n'nnGTC
Cuts at: 0 667 849
Size: 667 182
AleI CACnn'nnGTG
Cuts at: 0 842 849
Size: 842 7
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 535 567 849
Size: 535 32 282
Fragments arranged by size:
535 282 32
ApaI G_GGCC'C
Cuts at: 0 206 229 439 849
Size: 206 23 210 410
Fragments arranged by size:
410 210 206 23
AvaI C'yCGr_G
Cuts at: 0 153 440 484 849
Size: 153 287 44 365
Fragments arranged by size:
365 287 153 44
BanII G_rGCy'C
Cuts at: 0 139 206 229 307 439 471 849
Size: 139 67 23 78 132 32 378
Fragments arranged by size:
378 139 132 78 67 32 23
BbvCI CC'TCA_GC
Cuts at: 0 184 372 849
Size: 184 188 477
Fragments arranged by size:
477 188 184
Bme1580I G_kGCm'C
Cuts at: 0 86 206 229 439 849
Size: 86 120 23 210 410
Fragments arranged by size:
410 210 120 86 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 580 667 836 849
Size: 580 87 169 13
Fragments arranged by size:
580 169 87 13
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 723 849
Size: 723 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 499 849
Size: 124 375 350
Fragments arranged by size:
375 350 124
Bpu10I CC'TnA_GC
Cuts at: 0 184 372 708 849
Size: 184 188 336 141
Fragments arranged by size:
336 188 184 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 739 849
Size: 532 207 110
Fragments arranged by size:
532 207 110
BsaI GGTCTCn'nnnn_
Cuts at: 0 770 849
Size: 770 79
BsaAI yAC'GTr
Cuts at: 0 282 411 849
Size: 282 129 438
Fragments arranged by size:
438 282 129
BsaBI GATnn'nnATC
Cuts at: 0 328 849
Size: 328 521
BsaHI Gr'CG_yC
Cuts at: 0 71 849
Size: 71 778
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 688 718 849
Size: 688 30 131
Fragments arranged by size:
688 131 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 16 138 637 712 849
Size: 16 122 499 75 137
Fragments arranged by size:
499 137 122 75 16
BseYI C'CCAG_C
Cuts at: 0 19 206 849
Size: 19 187 643
Fragments arranged by size:
643 187 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 849
Size: 551 298
BsiHKAI G_wGCw'C
Cuts at: 0 709 849
Size: 709 140
Bsp1286I G_dGCh'C
Cuts at: 0 86 139 206 229 307 439 471 709
Size: 86 53 67 23 78 132 32 238
Cuts at: 709 849
Size: 140
Fragments arranged by size:
238 140 132 86 78 67 53 32
23
BsrBI CCG'CTC
Cuts at: 0 77 849
Size: 77 772
BsrFI r'CCGG_y
Cuts at: 0 736 849
Size: 736 113
BstXI CCAn_nnnn'nTGG
Cuts at: 0 102 198 219 249 597 664 849
Size: 102 96 21 30 348 67 185
Fragments arranged by size:
348 185 102 96 67 30 21
BtgI C'CryG_G
Cuts at: 0 341 849
Size: 341 508
BtsI GCAGTG_nn'
Cuts at: 0 837 849
Size: 837 12
ClaI AT'CG_AT
Cuts at: 0 196 849
Size: 196 653
DrdI GACnn_nn'nnGTC
Cuts at: 0 652 800 849
Size: 652 148 49
Fragments arranged by size:
652 148 49
EaeI y'GGCC_r
Cuts at: 0 530 734 849
Size: 530 204 115
Fragments arranged by size:
530 204 115
EarI CTCTTCn'nnn_
Cuts at: 0 614 849
Size: 614 235
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 262 849
Size: 262 587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 124 432 499 849
Size: 109 15 308 67 350
Fragments arranged by size:
350 308 109 67 15
EcoO109I rG'GnC_Cy
Cuts at: 0 202 203 225 435 436 456 669 714
Size: 202 1 22 210 1 20 213 45
Cuts at: 714 802 849
Size: 88 47
Fragments arranged by size:
213 210 202 88 47 45 22 20
1 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 124 156 653 685 849
Size: 61 32 31 32 497 32 164
Fragments arranged by size:
497 164 61 32 32 32 31
MslI CAynn'nnrTG
Cuts at: 0 196 842 849
Size: 196 646 7
Fragments arranged by size:
646 196 7
MspA1I CmG'CkG
Cuts at: 0 343 849
Size: 343 506
PasI CC'CwG_GG
Cuts at: 0 219 789 849
Size: 219 570 60
Fragments arranged by size:
570 219 60
PfoI T'CCnGG_A
Cuts at: 0 101 248 849
Size: 101 147 601
Fragments arranged by size:
601 147 101
PpuMI rG'GwC_Cy
Cuts at: 0 456 669 714 802 849
Size: 456 213 45 88 47
Fragments arranged by size:
456 213 88 47 45
PspOMI G'GGCC_C
Cuts at: 0 202 225 435 849
Size: 202 23 210 414
Fragments arranged by size:
414 210 202 23
SacII CC_GC'GG
Cuts at: 0 344 849
Size: 344 505
SanDI GG'GwC_CC
Cuts at: 0 669 802 849
Size: 669 133 47
Fragments arranged by size:
669 133 47
SmaI CCC'GGG
Cuts at: 0 442 849
Size: 442 407
SmlI C'TyrA_G
Cuts at: 0 547 718 849
Size: 547 171 131
Fragments arranged by size:
547 171 131
StuI AGG'CCT
Cuts at: 0 370 849
Size: 370 479
StyI C'CwwG_G
Cuts at: 0 747 849
Size: 747 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 553 849
Size: 553 296
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 688 720 849
Size: 688 32 129
Fragments arranged by size:
688 129 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 246 567 849
Size: 246 321 282
Fragments arranged by size:
321 282 246
XmaI C'CCGG_G
Cuts at: 0 440 849
Size: 440 409
Enzymes that do cut:
AcuI AhdI AleI AloI ApaI AvaI BanII BbvCI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BsrBI BsrFI BstXI BtgI BtsI ClaI DrdI EaeI
EarI EciI Eco57MI EcoO109I Hin4I MslI MspA1I PasI
PfoI PpuMI PspOMI SacII SanDI SmaI SmlI StuI
StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI CspCI DraI DraIII EagI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XhoI XmnI ZraI