Restriction map for
pFN21AB7114
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI ApoI AscI AseI
AsnI AspEI AspI AvaII AviII
AvrII BamHI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HindIII HpaI KspI MamI
MfeI MluI MroI MseI MunI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 75 enzymes) #
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Acc65I 697 GGTACC G^GTACC
AccII 94 CGCG CG^CG
AcyI 341 GGCGCC G[GA]^CG[CT]C
Alw44I 675 GTGCAC G^TGCAC
AlwI 499 GATCC GGATC(4/5)
ApaI 230 GGGCCC GGGCC^C
ApaLI 675 GTGCAC G^TGCAC
Asp700I 549 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 697 GGTACC G^GTACC
AvaI 791 CCCGAG C^[CT]CG[GA]G
BalI 572 TGGCCA TGG^CCA
BbeI 341 GGCGCC GGCGC^C
BbrPI 280 CACGTG CAC^GTG
BbsI 82 GTCTTC GAAGAC(2/6)
BfaI 403 CTAG C^TAG
BglI 167 GCCAGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 6 CTGGAG CTGGAG(16/14)
BpuAI 82 GTCTTC GAAGAC(2/6)
BsaAI 280 CACGTG [CT]AC^GT[GA]
BsaHI 341 GGCGCC G[GA]^CG[CT]C
BsaI 794 GAGACC GGTCTC(1/5)
BseRI 770 CTCCTC GAGGAG(10/8)
BsiEI 116 CGGCCG CG[GA][CT]^CG
BsoBI 791 CCCGAG C^[CT]CG[GA]G
BsrI 5 ACTGG ACTGG(1/-1)
BstAPI 629 GCAAGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 736 GGTGACC G^GT[ACGT]ACC
BstPI 736 GGTGACC G^GT[ACGT]ACC
BstUI 94 CGCG CG^CG
BstXI 509 CCAATATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 498 AGATCC [GA]^GATC[CT]
DpnII 499 GATC ^GATC
DraII 230 GGGCCCT [GA]G^G[ACGT]CC[CT]
EagI 116 CGGCCG C^GGCCG
EclXI 116 CGGCCG C^GGCCG
Eco52I 116 CGGCCG C^GGCCG
EcoO109I 230 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 736 GGTGACC G^GT[ACGT]ACC
EcoT14I 324 CCATGG C^C[AT][AT]GG
FauI 369 GCGGG CCCGC(4/6)
HaeII 341 GGCGCC [GA]GCGC^[CT]
Hin1I 341 GGCGCC G[GA]^CG[CT]C
HincII 37 GTCAAC GT[CT]^[GA]AC
HindII 37 GTCAAC GT[CT]^[GA]AC
HinfI 23 GACTC G^A[ACGT]TC
KasI 341 GGCGCC G^GCGCC
KpnI 697 GGTACC GGTAC^C
MaeI 403 CTAG C^TAG
MboI 499 GATC ^GATC
MflI 498 AGATCC [GA]^GATC[CT]
MluNI 572 TGGCCA TGG^CCA
MscI 572 TGGCCA TGG^CCA
MslI 276 CATGCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 578 CAGCTG C[AC]G^C[GT]G
MvnI 94 CGCG CG^CG
NarI 341 GGCGCC GG^CGCC
NcoI 324 CCATGG C^CATGG
NdeI 389 CATATG CA^TATG
NdeII 499 GATC ^GATC
NspI 253 ACATGC [GA]CATG^[CT]
PleI 23 GACTC GAGTC(4/5)
PmaCI 280 CACGTG CAC^GTG
PmlI 280 CACGTG CAC^GTG
PshAI 387 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 578 CAGCTG CAG^CTG
SacI 610 GAGCTC GAGCT^C
Sau3AI 499 GATC ^GATC
ScaI 44 AGTACT AGT^ACT
SfoI 341 GGCGCC GGC^GCC
SmlI 665 CTCAAG C^T[CT][GA]AG
StyI 324 CCATGG C^C[AT][AT]GG
TaqI 152 TCGA T^CGA
TthHB8I 152 TCGA T^CGA
XhoII 498 AGATCC [GA]^GATC[CT]
XmnI 549 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AspHI 610 GAGCTC G[AT]GC[AT]^C
675 GTGCAC
BsiHKAI 610 GAGCTC G[AT]GC[AT]^C
675 GTGCAC
BsmAI 794 GAGAC GTCTC(1/5)
75 GTCTC
BstF5I 591 CATCC GGATG(2/0)
766 CATCC
Cfr13I 230 GGGCC G^G[ACGT]CC
231 GGCCC
DsaI 324 CCATGG C^C[GA][CT]GG
693 CCACGG
EaeI 116 CGGCCG [CT]^GGCC[GA]
572 TGGCCA
FokI 591 CATCC GGATG(9/13)
766 CATCC
MaeII 185 ACGT A^CGT
281 ACGT
PstI 235 CTGCAG CTGCA^G
396 CTGCAG
Sau96I 230 GGGCC G^G[ACGT]CC
231 GGCCC
SexAI 107 ACCTGGT A^CC[AT]GGT
620 ACCTGGT
SfcI 235 CTGCAG C^T[GA][CT]AG
396 CTGCAG
TspRI 263 CACTG CA[GC]TG[ACGT][ACGT]^
336 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI AluI BanI BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BssSI
BstNI Cac8I CfoI DdeI EarI
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeIII MboII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV RsaI
ScrFI SfaNI TseI Tsp45I