Restriction map for pFN21AB7110
 (Linear) (Six-base) MAPSORT of: KIBB7110.seq  Check: 2165  from: 1  to: 780



KIBB7110.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     429     780 
   Size:        429     351
 
AlwNI CAG_nnn'CTG

Cuts at:      0     273     780 
   Size:        273     507
 
AseI AT'TA_AT

Cuts at:      0     766     780 
   Size:        766      14
 
BanI G'GyrC_C

Cuts at:      0      88     387     630     780 
   Size:         88     299     243     150

  Fragments arranged by size: 

                 299     243     150      88
 
BanII G_rGCy'C

Cuts at:      0     308     780 
   Size:        308     472
 
BbeI G_GCGC'C

Cuts at:      0      92     780 
   Size:         92     688
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     420     657     780 
   Size:        420     237     123

  Fragments arranged by size: 

                 420     237     123
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     307     341     780 
   Size:        307      34     439

  Fragments arranged by size: 

                 439     307      34
 
BglII A'GATC_T

Cuts at:      0     395     780 
   Size:        395     385
 
Bme1580I G_kGCm'C

Cuts at:      0     635     780 
   Size:        635     145
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     570     780 
   Size:        570     210
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     451     598     780 
   Size:        451     147     182

  Fragments arranged by size: 

                 451     182     147
 
BsaHI Gr'CG_yC

Cuts at:      0      89     780 
   Size:         89     691
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     391     421     780 
   Size:        391      30     359

  Fragments arranged by size: 

                 391     359      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     399     504     604     607     780 
   Size:        399     105     100       3     173

  Fragments arranged by size: 

                 399     173     105     100       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     379     780 
   Size:        286      93     401

  Fragments arranged by size: 

                 401     286      93
 
BsiHKAI G_wGCw'C

Cuts at:      0     308     780 
   Size:        308     472
 
BsmI GAATG_Cn'

Cuts at:      0     330     780 
   Size:        330     450
 
Bsp1286I G_dGCh'C

Cuts at:      0     308     635     780 
   Size:        308     327     145

  Fragments arranged by size: 

                 327     308     145
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     215     780 
   Size:        215     565
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     455     780 
   Size:        455     325
 
BstYI r'GATC_y

Cuts at:      0     395     780 
   Size:        395     385
 
Bsu36I CC'TnA_GG

Cuts at:      0     212     780 
   Size:        212     568
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     713     780 
   Size:        713      67
 
ClaI AT'CG_AT

Cuts at:      0     713     780 
   Size:        713      67
 
EaeI y'GGCC_r

Cuts at:      0     566     780 
   Size:        566     214
 
EarI CTCTTCn'nnn_

Cuts at:      0     417     466     493     668     780 
   Size:        417      49      27     175     112

  Fragments arranged by size: 

                 417     175     112      49      27
 
EcoICRI GAG'CTC

Cuts at:      0     306     780 
   Size:        306     474
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     429     780 
   Size:        429     351
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     211     277     586     780 
   Size:        211      66     309     194

  Fragments arranged by size: 

                 309     211     194      66
 
EcoO109I rG'GnC_Cy

Cuts at:      0     282     537     579     780 
   Size:        282     255      42     201

  Fragments arranged by size: 

                 282     255     201      42
 
HaeII r_GCGC'y

Cuts at:      0      92     780 
   Size:         92     688
 
KasI G'GCGC_C

Cuts at:      0      88     780 
   Size:         88     692
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     780 
   Size:         29     751
 
MscI TGG'CCA

Cuts at:      0     568     780 
   Size:        568     212
 
MslI CAynn'nnrTG

Cuts at:      0     728     780 
   Size:        728      52
 
MspA1I CmG'CkG

Cuts at:      0     101     780 
   Size:        101     679
 
NarI GG'CG_CC

Cuts at:      0      89     780 
   Size:         89     691
 
NspI r_CATG'y

Cuts at:      0     733     780 
   Size:        733      47
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     228     780 
   Size:        228     552
 
PfoI T'CCnGG_A

Cuts at:      0     221     780 
   Size:        221     559
 
PpuMI rG'GwC_Cy

Cuts at:      0     537     780 
   Size:        537     243
 
PstI C_TGCA'G

Cuts at:      0     290     780 
   Size:        290     490
 
SacI G_AGCT'C

Cuts at:      0     308     780 
   Size:        308     472
 
SapI GCTCTTCn'nnn_

Cuts at:      0     493     780 
   Size:        493     287
 
SbfI CC_TGCA'GG

Cuts at:      0     290     780 
   Size:        290     490
 
SexAI A'CCwGG_T

Cuts at:      0     260     780 
   Size:        260     520
 
SfcI C'TryA_G

Cuts at:      0     286     399     780 
   Size:        286     113     381

  Fragments arranged by size: 

                 381     286     113
 
SfoI GGC'GCC

Cuts at:      0      90     780 
   Size:         90     690
 
SmlI C'TyrA_G

Cuts at:      0     466     613     780 
   Size:        466     147     167

  Fragments arranged by size: 

                 466     167     147
 
StyI C'CwwG_G

Cuts at:      0     311     474     780 
   Size:        311     163     306

  Fragments arranged by size: 

                 311     306     163
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     552     615     740     780 
   Size:        552      63     125      40

  Fragments arranged by size: 

                 552     125      63      40
 
TatI w'GTAC_w

Cuts at:      0     596     780 
   Size:        596     184
 

 Enzymes that do cut:

     AcuI    AlwNI     AseI     BanI    BanII     BbeI     BbsI     BcgI
    BglII Bme1580I     BmrI    BpuEI    BsaHI    BsaXI    BseRI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI   BstAPI    BstYI   Bsu36I    BtgZI
     ClaI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII
     KasI     MmeI     MscI     MslI   MspA1I     NarI     NspI    PflMI
     PfoI    PpuMI     PstI     SacI     SapI     SbfI    SexAI     SfcI
     SfoI     SmlI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI    BbvCI    BciVI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BseYI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I     BtgI     BtsI    CspCI
     DraI   DraIII     DrdI     EagI     EciI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     ScaI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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