(Circular) (Six-base) MAPSORT of: pFN21AB7105.seq Check: 3373 from: 1 to: 5377 pFN21AB7105 5377 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2386 2386 Size: 5377 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3187 278 Size: 53 83 186 936 1651 2468 Fragments arranged by size: 2468 1651 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2720 1937 Size: 783 4594 Acc65I G'GTAC_C Cuts at: 2698 2698 Size: 5377 AclI AA'CG_TT Cuts at: 1563 3504 3877 1563 Size: 1941 373 3063 Fragments arranged by size: 3063 1941 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3440 4824 5092 1632 Size: 1808 1384 268 1917 Fragments arranged by size: 1917 1808 1384 268 AfeI AGC'GCT Cuts at: 2276 2276 Size: 5377 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5180 AflIII A'CryG_T Cuts at: 1203 4276 5253 1203 Size: 3073 977 1327 Fragments arranged by size: 3073 1327 977 AgeI A'CCGG_T Cuts at: 4208 4208 Size: 5377 AhdI GACnn_n'nnGTC Cuts at: 4106 4106 Size: 5377 AleI CACnn'nnGTG Cuts at: 1166 2153 2219 2342 1166 Size: 987 66 123 4201 Fragments arranged by size: 4201 987 123 66 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5377 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2157 2189 2157 Size: 32 5345 AlwNI CAG_nnn'CTG Cuts at: 3120 4692 3120 Size: 1572 3805 ApaI G_GGCC'C Cuts at: 2049 2094 2326 2420 2466 2049 Size: 45 232 94 46 4960 Fragments arranged by size: 4960 232 94 46 45 ApaLI G'TGCA_C Cuts at: 2208 3434 4590 2208 Size: 1226 1156 2995 Fragments arranged by size: 2995 1226 1156 ApoI r'AATT_y Cuts at: 1493 2395 2686 2912 3064 5173 1493 Size: 902 291 226 152 2109 1697 Fragments arranged by size: 2109 1697 902 291 226 152 AseI AT'TA_AT Cuts at: 160 1032 3931 160 Size: 872 2899 1606 Fragments arranged by size: 2899 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5377 AvaI C'yCGr_G Cuts at: 1870 1943 2268 2361 2519 2702 1870 Size: 73 325 93 158 183 4545 Fragments arranged by size: 4545 325 183 158 93 73 BamHI G'GATC_C Cuts at: 2590 2707 3074 2590 Size: 117 367 4893 Fragments arranged by size: 4893 367 117 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2226 2261 2698 4153 618 Size: 325 217 627 439 35 437 1455 1842 Fragments arranged by size: 1842 1455 627 439 437 325 217 35 BanII G_rGCy'C Cuts at: 1391 2049 2094 2256 2326 2420 2466 2558 2588 Size: 658 45 162 70 94 46 92 30 Cuts at: 2588 2696 1391 Size: 108 4072 Fragments arranged by size: 4072 658 162 108 94 92 70 46 45 30 BbeI G_GCGC'C Cuts at: 2230 2230 Size: 5377 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4406 BbvCI CC'TCA_GC Cuts at: 2386 2386 Size: 5377 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3568 3602 1903 Size: 34 50 34 1547 34 3678 Fragments arranged by size: 3678 1547 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3269 4479 3269 Size: 1210 4167 BclI T'GATC_A Cuts at: 1548 1548 Size: 5377 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2032 2089 2126 Size: 107 122 71 631 605 360 57 37 Cuts at: 2126 3988 136 Size: 1862 1525 Fragments arranged by size: 1862 1525 631 605 360 122 107 71 57 37 BglII A'GATC_T Cuts at: 2631 5372 2631 Size: 2741 2636 BlpI GC'TnA_GC Cuts at: 2794 2794 Size: 5377 Bme1580I G_kGCm'C Cuts at: 1790 2049 2094 2212 2264 2326 2351 2420 2466 Size: 259 45 118 52 62 25 69 46 Cuts at: 2466 3438 4594 1790 Size: 972 1156 2573 Fragments arranged by size: 2573 1156 972 259 118 69 62 52 46 45 25 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5377 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3108 4066 442 Size: 416 53 83 399 24 1691 958 1753 Fragments arranged by size: 1753 1691 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5377 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2044 2650 2044 Size: 606 4771 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2001 2453 2591 4037 1239 Size: 762 452 138 1446 2579 Fragments arranged by size: 2579 1446 762 452 138 Bpu10I CC'TnA_GC Cuts at: 2328 2386 2328 Size: 58 5319 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3502 4367 4665 3502 Size: 865 298 4214 Fragments arranged by size: 4214 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4040 882 Size: 617 2541 2219 Fragments arranged by size: 2541 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3168 493 Size: 711 1964 2702 Fragments arranged by size: 2702 1964 711 BsaBI GATnn'nnATC Cuts at: 5371 5371 Size: 5377 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2227 3184 3566 4201 Size: 53 83 186 936 694 957 382 635 Cuts at: 4201 275 Size: 1451 Fragments arranged by size: 1451 957 936 694 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3810 4208 4482 4629 5040 3810 Size: 398 274 147 411 4147 Fragments arranged by size: 4147 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5152 Fragments arranged by size: 5152 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2250 1186 Size: 1064 4313 BseYI C'CCAG_C Cuts at: 2198 2405 2440 2466 2508 4580 5076 2198 Size: 207 35 26 42 2072 496 2499 Fragments arranged by size: 2499 2072 496 207 42 35 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4804 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2827 3588 4616 1540 Size: 275 186 826 761 1028 2301 Fragments arranged by size: 2301 1028 826 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2185 2212 2453 3438 3523 4594 2185 Size: 27 241 985 85 1071 2968 Fragments arranged by size: 2968 1071 985 241 85 27 BsmI GAATG_Cn' Cuts at: 2888 2981 2888 Size: 93 5284 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2094 2185 2212 2256 2264 2326 Size: 399 259 45 91 27 44 8 62 Cuts at: 2326 2351 2420 2453 2466 2558 2588 2696 3438 Size: 25 69 33 13 92 30 108 742 Cuts at: 3438 3523 4594 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 259 108 92 91 85 69 62 45 44 33 30 27 25 13 8 BspEI T'CCGG_A Cuts at: 5040 5040 Size: 5377 BspHI T'CATG_A Cuts at: 3266 4996 3266 Size: 1730 3647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2386 2732 844 Size: 1542 346 3489 Fragments arranged by size: 3489 1542 346 BsrBI CCG'CTC Cuts at: 1394 1823 3264 1394 Size: 429 1441 3507 Fragments arranged by size: 3507 1441 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3872 4046 65 Size: 1183 2624 174 1396 Fragments arranged by size: 2624 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4021 4208 1700 Size: 110 2211 187 2869 Fragments arranged by size: 2869 2211 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5377 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5377 BssSI C'ACGA_G Cuts at: 3437 4449 3437 Size: 1012 4365 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5377 BstBI TT'CG_AA Cuts at: 4181 4181 Size: 5377 BstEII G'GTnAC_C Cuts at: 1184 2617 1184 Size: 1433 3944 BstXI CCAn_nnnn'nTGG Cuts at: 2110 2344 2413 2500 2110 Size: 234 69 87 4987 Fragments arranged by size: 4987 234 87 69 BstYI r'GATC_y Cuts at: 1972 2590 2631 2707 3074 3459 3476 4928 5014 Size: 618 41 76 367 385 17 1452 86 Cuts at: 5014 5026 5372 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 618 385 367 346 86 76 41 17 12 BtgI C'CryG_G Cuts at: 513 1065 2069 2256 2612 513 Size: 552 1004 187 356 3278 Fragments arranged by size: 3278 1004 552 356 187 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4638 Fragments arranged by size: 4638 683 56 BtsI GCAGTG_nn' Cuts at: 2078 2893 3687 3707 2078 Size: 815 794 20 3748 Fragments arranged by size: 3748 815 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5377 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5342 DraI TTT'AAA Cuts at: 2681 2681 Size: 5377 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4384 809 Size: 507 3068 1802 Fragments arranged by size: 3068 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 2576 2824 Size: 54 1425 50 275 18 288 458 248 Cuts at: 2824 3713 5342 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 458 288 275 248 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2824 1537 Size: 275 1012 4090 Fragments arranged by size: 4090 1012 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2141 3307 1353 Size: 788 1166 3423 Fragments arranged by size: 3423 1166 788 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2165 2177 3964 4338 4484 387 Size: 1064 138 576 12 1787 374 146 1280 Fragments arranged by size: 1787 1280 1064 576 374 146 138 12 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2001 2453 2591 3440 4037 4824 5092 Size: 393 369 452 138 849 597 787 268 Cuts at: 5092 1239 Size: 1524 Fragments arranged by size: 1524 849 787 597 452 393 369 268 138 EcoO109I rG'GnC_Cy Cuts at: 2045 2046 2091 2323 2417 2437 2462 2463 2484 Size: 1 45 232 94 20 25 1 21 Cuts at: 2484 2602 2045 Size: 118 4820 Fragments arranged by size: 4820 232 118 94 45 25 21 20 1 1 EcoRI G'AATT_C Cuts at: 2686 3064 2686 Size: 378 4999 FspI TGC'GCA Cuts at: 3883 3883 Size: 5377 HaeII r_GCGC'y Cuts at: 1678 2230 2278 4524 1678 Size: 552 48 2246 2531 Fragments arranged by size: 2531 2246 552 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2575 2607 4018 4050 4092 Size: 32 135 32 1003 32 1411 32 42 Cuts at: 4092 4124 1373 Size: 32 2626 Fragments arranged by size: 2626 1411 1003 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2013 2721 2967 669 Size: 988 281 75 708 246 3079 Fragments arranged by size: 3079 988 708 281 246 75 HindIII A'AGCT_T Cuts at: 748 748 Size: 5377 HpaI GTT'AAC Cuts at: 2967 2967 Size: 5377 KasI G'GCGC_C Cuts at: 2226 2226 Size: 5377 KpnI G_GTAC'C Cuts at: 2702 2702 Size: 5377 MfeI C'AATT_G Cuts at: 2976 2976 Size: 5377 MluI A'CGCG_T Cuts at: 5253 5253 Size: 5377 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4491 4675 1305 Size: 3186 184 2007 Fragments arranged by size: 3186 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2120 10 Size: 54 1425 343 288 3267 Fragments arranged by size: 3267 1425 343 288 54 MslI CAynn'nnrTG Cuts at: 518 1166 2153 2219 2342 3335 3694 3853 518 Size: 648 987 66 123 993 359 159 2042 Fragments arranged by size: 2042 993 987 648 359 159 123 66 MspA1I CmG'CkG Cuts at: 1606 2153 2575 2793 3470 4618 4863 1606 Size: 547 422 218 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 547 422 245 218 NarI GG'CG_CC Cuts at: 2227 2227 Size: 5377 NcoI C'CATG_G Cuts at: 513 1065 2069 2256 513 Size: 552 1004 187 3634 Fragments arranged by size: 3634 1004 552 187 NdeI CA'TA_TG Cuts at: 387 387 Size: 5377 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5377 NotI GC'GGCC_GC Cuts at: 2824 2824 Size: 5377 NspI r_CATG'y Cuts at: 1132 1229 2735 4280 1132 Size: 97 1506 1545 2229 Fragments arranged by size: 2229 1545 1506 97 PasI CC'CwG_GG Cuts at: 1348 2472 1348 Size: 1124 4253 PciI A'CATG_T Cuts at: 4276 4276 Size: 5377 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5377 PmeI GTTT'AAAC Cuts at: 2681 2681 Size: 5377 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3446 3478 4959 4991 3446 Size: 32 1481 32 3832 Fragments arranged by size: 3832 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2437 2437 Size: 5377 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5377 PsiI TTA'TAA Cuts at: 2947 2947 Size: 5377 PspOMI G'GGCC_C Cuts at: 2045 2090 2322 2416 2462 2045 Size: 45 232 94 46 4960 Fragments arranged by size: 4960 232 94 46 45 PstI C_TGCA'G Cuts at: 830 2729 830 Size: 1899 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5377 SalI G'TCGA_C Cuts at: 1936 2719 1936 Size: 783 4594 SapI GCTCTTCn'nnn_ Cuts at: 2141 2141 Size: 5377 SbfI CC_TGCA'GG Cuts at: 2729 2729 Size: 5377 ScaI AGT'ACT Cuts at: 3625 5337 3625 Size: 1712 3665 SfcI C'TryA_G Cuts at: 826 1045 2725 3860 4541 4732 826 Size: 219 1680 1135 681 191 1471 Fragments arranged by size: 1680 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2228 2228 Size: 5377 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5377 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5377 SmaI CCC'GGG Cuts at: 1872 2704 1872 Size: 832 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3481 4382 4644 820 Size: 197 926 1538 901 262 1553 Fragments arranged by size: 1553 1538 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3168 493 Size: 2675 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5377 SphI G_CATG'C Cuts at: 1132 2735 1132 Size: 1603 3774 SspI AAT'ATT Cuts at: 5 52 3301 5 Size: 47 3249 2081 Fragments arranged by size: 3249 2081 47 StuI AGG'CCT Cuts at: 1517 2499 1517 Size: 982 4395 StyI C'CwwG_G Cuts at: 513 1065 2069 2256 2622 2816 513 Size: 552 1004 187 366 194 3074 Fragments arranged by size: 3074 1004 552 366 194 187 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2227 2354 3404 3421 3574 3759 4204 Size: 1255 647 127 1050 17 153 185 445 Cuts at: 4204 325 Size: 1498 Fragments arranged by size: 1498 1255 1050 647 445 185 153 127 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3623 5335 96 Size: 274 80 33 51 1443 1646 1712 138 Fragments arranged by size: 1712 1646 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2582 2614 5337 5369 2582 Size: 32 2723 32 2590 Fragments arranged by size: 2723 2590 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5377 XbaI T'CTAG_A Cuts at: 2713 2713 Size: 5377 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2155 2155 Size: 5377 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5377 XmaI C'CCGG_G Cuts at: 1870 2702 1870 Size: 832 4545 XmnI GAAnn'nnTTC Cuts at: 3506 3506 Size: 5377 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3185 276 Size: 53 83 186 936 1651 2468 Fragments arranged by size: 2468 1651 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI