Restriction map for pFN21AB7105
 (Circular) (Six-base) MAPSORT of: pFN21AB7105.seq  Check: 3373  from: 1  to: 5377



pFN21AB7105  5377 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2386    2386 
   Size:       5377
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3187     278 
   Size:         53      83     186     936    1651    2468

  Fragments arranged by size: 

                2468    1651     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2720    1937 
   Size:        783    4594
 
Acc65I G'GTAC_C

Cuts at:   2698    2698 
   Size:       5377
 
AclI AA'CG_TT

Cuts at:   1563    3504    3877    1563 
   Size:       1941     373    3063

  Fragments arranged by size: 

                3063    1941     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3440    4824    5092    1632 
   Size:       1808    1384     268    1917

  Fragments arranged by size: 

                1917    1808    1384     268
 
AfeI AGC'GCT

Cuts at:   2276    2276 
   Size:       5377
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5180
 
AflIII A'CryG_T

Cuts at:   1203    4276    5253    1203 
   Size:       3073     977    1327

  Fragments arranged by size: 

                3073    1327     977
 
AgeI A'CCGG_T

Cuts at:   4208    4208 
   Size:       5377
 
AhdI GACnn_n'nnGTC

Cuts at:   4106    4106 
   Size:       5377
 
AleI CACnn'nnGTG

Cuts at:   1166    2153    2219    2342    1166 
   Size:        987      66     123    4201

  Fragments arranged by size: 

                4201     987     123      66
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5377
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2157    2189    2157 
   Size:         32    5345
 
AlwNI CAG_nnn'CTG

Cuts at:   3120    4692    3120 
   Size:       1572    3805
 
ApaI G_GGCC'C

Cuts at:   2049    2094    2326    2420    2466    2049 
   Size:         45     232      94      46    4960

  Fragments arranged by size: 

                4960     232      94      46      45
 
ApaLI G'TGCA_C

Cuts at:   2208    3434    4590    2208 
   Size:       1226    1156    2995

  Fragments arranged by size: 

                2995    1226    1156
 
ApoI r'AATT_y

Cuts at:   1493    2395    2686    2912    3064    5173    1493 
   Size:        902     291     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     902     291     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3931     160 
   Size:        872    2899    1606

  Fragments arranged by size: 

                2899    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5377
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2268    2361    2519    2702    1870 
   Size:         73     325      93     158     183    4545

  Fragments arranged by size: 

                4545     325     183     158      93      73
 
BamHI G'GATC_C

Cuts at:   2590    2707    3074    2590 
   Size:        117     367    4893

  Fragments arranged by size: 

                4893     367     117
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2226    2261    2698    4153     618 
   Size:        325     217     627     439      35     437    1455    1842

  Fragments arranged by size: 

                1842    1455     627     439     437     325     217      35
            
 
BanII G_rGCy'C

Cuts at:   1391    2049    2094    2256    2326    2420    2466    2558    2588 
   Size:        658      45     162      70      94      46      92      30

Cuts at:   2588    2696    1391 
   Size:        108    4072

  Fragments arranged by size: 

                4072     658     162     108      94      92      70      46
                  45      30
 
BbeI G_GCGC'C

Cuts at:   2230    2230 
   Size:       5377
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4406
 
BbvCI CC'TCA_GC

Cuts at:   2386    2386 
   Size:       5377
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    3568    3602    1903 
   Size:         34      50      34    1547      34    3678

  Fragments arranged by size: 

                3678    1547      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3269    4479    3269 
   Size:       1210    4167
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5377
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2032    2089    2126 
   Size:        107     122      71     631     605     360      57      37

Cuts at:   2126    3988     136 
   Size:       1862    1525

  Fragments arranged by size: 

                1862    1525     631     605     360     122     107      71
                  57      37
 
BglII A'GATC_T

Cuts at:   2631    5372    2631 
   Size:       2741    2636
 
BlpI GC'TnA_GC

Cuts at:   2794    2794 
   Size:       5377
 
Bme1580I G_kGCm'C

Cuts at:   1790    2049    2094    2212    2264    2326    2351    2420    2466 
   Size:        259      45     118      52      62      25      69      46

Cuts at:   2466    3438    4594    1790 
   Size:        972    1156    2573

  Fragments arranged by size: 

                2573    1156     972     259     118      69      62      52
                  46      45      25
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5377
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3108    4066     442 
   Size:        416      53      83     399      24    1691     958    1753

  Fragments arranged by size: 

                1753    1691     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5377
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2044    2650    2044 
   Size:        606    4771
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2001    2453    2591    4037    1239 
   Size:        762     452     138    1446    2579

  Fragments arranged by size: 

                2579    1446     762     452     138
 
Bpu10I CC'TnA_GC

Cuts at:   2328    2386    2328 
   Size:         58    5319
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3502    4367    4665    3502 
   Size:        865     298    4214

  Fragments arranged by size: 

                4214     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4040     882 
   Size:        617    2541    2219

  Fragments arranged by size: 

                2541    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3168     493 
   Size:        711    1964    2702

  Fragments arranged by size: 

                2702    1964     711
 
BsaBI GATnn'nnATC

Cuts at:   5371    5371 
   Size:       5377
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2227    3184    3566    4201 
   Size:         53      83     186     936     694     957     382     635

Cuts at:   4201     275 
   Size:       1451

  Fragments arranged by size: 

                1451     957     936     694     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3810    4208    4482    4629    5040    3810 
   Size:        398     274     147     411    4147

  Fragments arranged by size: 

                4147     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5152

  Fragments arranged by size: 

                5152     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2250    1186 
   Size:       1064    4313
 
BseYI C'CCAG_C

Cuts at:   2198    2405    2440    2466    2508    4580    5076    2198 
   Size:        207      35      26      42    2072     496    2499

  Fragments arranged by size: 

                2499    2072     496     207      42      35      26
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4804
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2827    3588    4616    1540 
   Size:        275     186     826     761    1028    2301

  Fragments arranged by size: 

                2301    1028     826     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2185    2212    2453    3438    3523    4594    2185 
   Size:         27     241     985      85    1071    2968

  Fragments arranged by size: 

                2968    1071     985     241      85      27
 
BsmI GAATG_Cn'

Cuts at:   2888    2981    2888 
   Size:         93    5284
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2049    2094    2185    2212    2256    2264    2326 
   Size:        399     259      45      91      27      44       8      62

Cuts at:   2326    2351    2420    2453    2466    2558    2588    2696    3438 
   Size:         25      69      33      13      92      30     108     742

Cuts at:   3438    3523    4594    1391 
   Size:         85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     259     108      92      91
                  85      69      62      45      44      33      30      27
                  25      13       8
 
BspEI T'CCGG_A

Cuts at:   5040    5040 
   Size:       5377
 
BspHI T'CATG_A

Cuts at:   3266    4996    3266 
   Size:       1730    3647
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2386    2732     844 
   Size:       1542     346    3489

  Fragments arranged by size: 

                3489    1542     346
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3264    1394 
   Size:        429    1441    3507

  Fragments arranged by size: 

                3507    1441     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3872    4046      65 
   Size:       1183    2624     174    1396

  Fragments arranged by size: 

                2624    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4021    4208    1700 
   Size:        110    2211     187    2869

  Fragments arranged by size: 

                2869    2211     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5377
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5377
 
BssSI C'ACGA_G

Cuts at:   3437    4449    3437 
   Size:       1012    4365
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5377
 
BstBI TT'CG_AA

Cuts at:   4181    4181 
   Size:       5377
 
BstEII G'GTnAC_C

Cuts at:   1184    2617    1184 
   Size:       1433    3944
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2110    2344    2413    2500    2110 
   Size:        234      69      87    4987

  Fragments arranged by size: 

                4987     234      87      69
 
BstYI r'GATC_y

Cuts at:   1972    2590    2631    2707    3074    3459    3476    4928    5014 
   Size:        618      41      76     367     385      17    1452      86

Cuts at:   5014    5026    5372    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     618     385     367     346      86      76
                  41      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2069    2256    2612     513 
   Size:        552    1004     187     356    3278

  Fragments arranged by size: 

                3278    1004     552     356     187
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4638

  Fragments arranged by size: 

                4638     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2078    2893    3687    3707    2078 
   Size:        815     794      20    3748

  Fragments arranged by size: 

                3748     815     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5377
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5342
 
DraI TTT'AAA

Cuts at:   2681    2681 
   Size:       5377
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4384     809 
   Size:        507    3068    1802

  Fragments arranged by size: 

                3068    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2118    2576    2824 
   Size:         54    1425      50     275      18     288     458     248

Cuts at:   2824    3713    5342       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     458     288     275     248      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2824    1537 
   Size:        275    1012    4090

  Fragments arranged by size: 

                4090    1012     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2141    3307    1353 
   Size:        788    1166    3423

  Fragments arranged by size: 

                3423    1166     788
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2165    2177    3964    4338    4484     387 
   Size:       1064     138     576      12    1787     374     146    1280

  Fragments arranged by size: 

                1787    1280    1064     576     374     146     138      12
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2001    2453    2591    3440    4037    4824    5092 
   Size:        393     369     452     138     849     597     787     268

Cuts at:   5092    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     849     787     597     452     393     369     268
                 138
 
EcoO109I rG'GnC_Cy

Cuts at:   2045    2046    2091    2323    2417    2437    2462    2463    2484 
   Size:          1      45     232      94      20      25       1      21

Cuts at:   2484    2602    2045 
   Size:        118    4820

  Fragments arranged by size: 

                4820     232     118      94      45      25      21      20
                   1       1
 
EcoRI G'AATT_C

Cuts at:   2686    3064    2686 
   Size:        378    4999
 
FspI TGC'GCA

Cuts at:   3883    3883 
   Size:       5377
 
HaeII r_GCGC'y

Cuts at:   1678    2230    2278    4524    1678 
   Size:        552      48    2246    2531

  Fragments arranged by size: 

                2531    2246     552      48
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2575    2607    4018    4050    4092 
   Size:         32     135      32    1003      32    1411      32      42

Cuts at:   4092    4124    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1411    1003     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2013    2721    2967     669 
   Size:        988     281      75     708     246    3079

  Fragments arranged by size: 

                3079     988     708     281     246      75
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5377
 
HpaI GTT'AAC

Cuts at:   2967    2967 
   Size:       5377
 
KasI G'GCGC_C

Cuts at:   2226    2226 
   Size:       5377
 
KpnI G_GTAC'C

Cuts at:   2702    2702 
   Size:       5377
 
MfeI C'AATT_G

Cuts at:   2976    2976 
   Size:       5377
 
MluI A'CGCG_T

Cuts at:   5253    5253 
   Size:       5377
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4491    4675    1305 
   Size:       3186     184    2007

  Fragments arranged by size: 

                3186    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2120      10 
   Size:         54    1425     343     288    3267

  Fragments arranged by size: 

                3267    1425     343     288      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2153    2219    2342    3335    3694    3853     518 
   Size:        648     987      66     123     993     359     159    2042

  Fragments arranged by size: 

                2042     993     987     648     359     159     123      66
            
 
MspA1I CmG'CkG

Cuts at:   1606    2153    2575    2793    3470    4618    4863    1606 
   Size:        547     422     218     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     547     422     245     218
 
NarI GG'CG_CC

Cuts at:   2227    2227 
   Size:       5377
 
NcoI C'CATG_G

Cuts at:    513    1065    2069    2256     513 
   Size:        552    1004     187    3634

  Fragments arranged by size: 

                3634    1004     552     187
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5377
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5377
 
NotI GC'GGCC_GC

Cuts at:   2824    2824 
   Size:       5377
 
NspI r_CATG'y

Cuts at:   1132    1229    2735    4280    1132 
   Size:         97    1506    1545    2229

  Fragments arranged by size: 

                2229    1545    1506      97
 
PasI CC'CwG_GG

Cuts at:   1348    2472    1348 
   Size:       1124    4253
 
PciI A'CATG_T

Cuts at:   4276    4276 
   Size:       5377
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5377
 
PmeI GTTT'AAAC

Cuts at:   2681    2681 
   Size:       5377
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3446    3478    4959    4991    3446 
   Size:         32    1481      32    3832

  Fragments arranged by size: 

                3832    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2437    2437 
   Size:       5377
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5377
 
PsiI TTA'TAA

Cuts at:   2947    2947 
   Size:       5377
 
PspOMI G'GGCC_C

Cuts at:   2045    2090    2322    2416    2462    2045 
   Size:         45     232      94      46    4960

  Fragments arranged by size: 

                4960     232      94      46      45
 
PstI C_TGCA'G

Cuts at:    830    2729     830 
   Size:       1899    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5377
 
SalI G'TCGA_C

Cuts at:   1936    2719    1936 
   Size:        783    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2141    2141 
   Size:       5377
 
SbfI CC_TGCA'GG

Cuts at:   2729    2729 
   Size:       5377
 
ScaI AGT'ACT

Cuts at:   3625    5337    3625 
   Size:       1712    3665
 
SfcI C'TryA_G

Cuts at:    826    1045    2725    3860    4541    4732     826 
   Size:        219    1680    1135     681     191    1471

  Fragments arranged by size: 

                1680    1471    1135     681     219     191
 
SfoI GGC'GCC

Cuts at:   2228    2228 
   Size:       5377
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5377
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5377
 
SmaI CCC'GGG

Cuts at:   1872    2704    1872 
   Size:        832    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3481    4382    4644     820 
   Size:        197     926    1538     901     262    1553

  Fragments arranged by size: 

                1553    1538     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3168     493 
   Size:       2675    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5377
 
SphI G_CATG'C

Cuts at:   1132    2735    1132 
   Size:       1603    3774
 
SspI AAT'ATT

Cuts at:      5      52    3301       5 
   Size:         47    3249    2081

  Fragments arranged by size: 

                3249    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2499    1517 
   Size:        982    4395
 
StyI C'CwwG_G

Cuts at:    513    1065    2069    2256    2622    2816     513 
   Size:        552    1004     187     366     194    3074

  Fragments arranged by size: 

                3074    1004     552     366     194     187
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2227    2354    3404    3421    3574    3759    4204 
   Size:       1255     647     127    1050      17     153     185     445

Cuts at:   4204     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255    1050     647     445     185     153     127
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3623    5335      96 
   Size:        274      80      33      51    1443    1646    1712     138

  Fragments arranged by size: 

                1712    1646    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2582    2614    5337    5369    2582 
   Size:         32    2723      32    2590

  Fragments arranged by size: 

                2723    2590      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5377
 
XbaI T'CTAG_A

Cuts at:   2713    2713 
   Size:       5377
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2155    2155 
   Size:       5377
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5377
 
XmaI C'CCGG_G

Cuts at:   1870    2702    1870 
   Size:        832    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3506    3506 
   Size:       5377
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3185     276 
   Size:         53      83     186     936    1651    2468

  Fragments arranged by size: 

                2468    1651     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI
     PvuI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AscI    AvrII     BaeI    BfrBI    BsiWI    BsmBI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     NaeI
   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI    PspXI     PsrI
    PvuII    RsrII     SacI    SacII    SanDI    SexAI     SfiI     SrfI
     SwaI
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