Restriction map for
pFN21AB7091
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbsI BbvCI
BcgI BciVI BfrI BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MseI MspA1I MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 319 GTAGAC GT^[AC][GT]AC
AccII 172 CGCG CG^CG
AfaI 144 GTAC GT^AC
AflIII 434 ACGTGT A^C[GA][CT]GT
AlwNI 274 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 121 GAGCTC G[AT]GC[AT]^C
AvaII 183 GGACC G^G[AT]CC
BalI 197 TGGCCA TGG^CCA
BanI 289 GGTGCC G^G[CT][GA]CC
BanII 121 GAGCTC G[GA]GC[CT]^C
BbrPI 433 CACGTG CAC^GTG
BcnI 369 CCGGG CC^[GC]GG
BglI 292 GCCATGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 509 AGATCT A^GATCT
BmyI 121 GAGCTC G[AGT]GC[ACT]^C
BsaAI 433 CACGTG [CT]AC^GT[GA]
BsgI 217 CTGCAC GTGCAG(16/14)
BsiHKAI 121 GAGCTC G[AT]GC[AT]^C
BsmAI 97 GAGAC GTCTC(1/5)
Bsp1286I 121 GAGCTC G[AGT]GC[ACT]^C
BsrDI 224 GCAATG GCAATG(2/0)
BsrFI 155 GCCGGC [GA]^CCGG[CT]
BsrI 242 CCAGT ACTGG(1/-1)
BstUI 172 CGCG CG^CG
BstYI 509 AGATCT [GA]^GATC[CT]
CfoI 171 GCGC GCG^C
Cfr10I 155 GCCGGC [GA]^CCGG[CT]
DraII 182 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 85 CTCTTC CTCTTC(1/4)
EcoO109I 182 AGGACCT [GA]G^G[ACGT]CC[CT]
HhaI 171 GCGC GCG^C
HinP1I 171 GCGC G^CGC
Ksp632I 85 CTCTTC CTCTTC(1/4)
MaeII 434 ACGT A^CGT
MflI 509 AGATCT [GA]^GATC[CT]
MluNI 197 TGGCCA TGG^CCA
MscI 197 TGGCCA TGG^CCA
MvnI 172 CGCG CG^CG
NaeI 155 GCCGGC GCC^GGC
NciI 369 CCGGG CC^[GC]GG
NgoMIV 155 GCCGGC G^CCGGC
PmaCI 433 CACGTG CAC^GTG
PmlI 433 CACGTG CAC^GTG
PpuMI 182 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 454 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 144 GTAC GT^AC
SacI 121 GAGCTC GAGCT^C
SfaNI 425 GCATC GCATC(5/9)
TaqI 332 TCGA T^CGA
TthHB8I 332 TCGA T^CGA
XhoII 509 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BstF5I 426 CATCC GGATG(2/0)
372 GGATG
BstXI 200 CCATCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
242 CCAGTGAGCTGG
EaeI 197 TGGCCA [CT]^GGCC[GA]
420 TGGCCG
EcoT14I 137 CCAAGG C^C[AT][AT]GG
501 CCTTGG
FauI 263 CCCGC CCCGC(4/6)
64 GCGGG
FokI 426 CATCC GGATG(9/13)
372 GGATG
HapII 156 CCGG C^CGG
369 CCGG
HinfI 4 GAGTC G^A[ACGT]TC
521 GACTC
HpaII 156 CCGG C^CGG
369 CCGG
HphI 313 GGTGA GGTGA(8/7)
326 TCACC
MaeIII 414 GTCAC ^GT[ACGT]AC
515 GTGAC
MboII 42 GAAGA GAAGA(8/7)
86 TCTTC
MslI 201 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
220 CACCGCAATG
MspI 156 CCGG C^CGG
369 CCGG
NlaIII 294 CATG CATG^
418 CATG
PleI 521 GACTC GAGTC(4/5)
4 GAGTC
StyI 137 CCAAGG C^C[AT][AT]GG
501 CCTTGG
Tsp45I 414 GTCAC ^GT[GC]AC
515 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI BbvI BclI BfaI
BsaJI BsiYI BslI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
FbaI Fnu4HI HaeIII ItaI MaeI
MboI MnlI MvaI MwoI NdeII
NlaIV Sau3AI Sau96I ScrFI TseI
TspRI