Restriction map for
pFN21AB7087
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr13I ClaI CpoI DdeI DraI
DraII DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MunI
NarI NciI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SalI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 142 GGTACC G^GTACC
AccII 216 CGCG CG^CG
AfaI 143 GTAC GT^AC
AlwI 24 GATCC GGATC(4/5)
Asp718I 142 GGTACC G^GTACC
AspHI 148 GAGCTC G[AT]GC[AT]^C
BanI 142 GGTACC G^G[CT][GA]CC
BbsI 190 GTCTTC GAAGAC(2/6)
BclI 89 TGATCA T^GATCA
BfaI 298 CTAG C^TAG
BglI 34 GCCCCAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 303 CTCCAG CTGGAG(16/14)
BpuAI 190 GTCTTC GAAGAC(2/6)
BsiHKAI 148 GAGCTC G[AT]GC[AT]^C
BsmBI 351 GAGACG CGTCTC(1/5)
BsmFI 100 GTCCC GGGAC(10/14)
BsrFI 199 GCCGGC [GA]^CCGG[CT]
BsrI 305 CCAGT ACTGG(1/-1)
BssSI 373 CTCGTG CACGAG(-5/-1)
BstEII 210 GGTCACC G^GT[ACGT]ACC
BstF5I 171 CATCC GGATG(2/0)
BstPI 210 GGTCACC G^GT[ACGT]ACC
BstUI 216 CGCG CG^CG
BstYI 23 AGATCC [GA]^GATC[CT]
Cac8I 199 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 199 GCCGGC [GA]^CCGG[CT]
DrdI 353 GACGGGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 210 GGTCACC G^GT[ACGT]ACC
EcoT14I 240 CCATGG C^C[AT][AT]GG
FbaI 89 TGATCA T^GATCA
FokI 171 CATCC GGATG(9/13)
HaeIII 265 GGCC GG^CC
HapII 200 CCGG C^CGG
HindIII 390 AAGCTT A^AGCTT
HpaII 200 CCGG C^CGG
KpnI 142 GGTACC GGTAC^C
KspI 215 CCGCGG CCGC^GG
MaeI 298 CTAG C^TAG
MaeIII 211 GTCAC ^GT[ACGT]AC
MflI 23 AGATCC [GA]^GATC[CT]
MslI 213 CACCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 200 CCGG C^CGG
MvnI 216 CGCG CG^CG
NaeI 199 GCCGGC GCC^GGC
NcoI 240 CCATGG C^CATGG
NgoMIV 199 GCCGGC G^CCGGC
PleI 360 GAGTC GAGTC(4/5)
RsaI 143 GTAC GT^AC
SacI 148 GAGCTC GAGCT^C
SacII 215 CCGCGG CCGC^GG
SfcI 134 CTACAG C^T[GA][CT]AG
StyI 240 CCATGG C^C[AT][AT]GG
TfiI 117 GATTC G^A[AT]TC
Tsp45I 211 GTCAC ^GT[GC]AC
Tsp509I 235 AATT ^AATT
XhoII 23 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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BsiYI 311 CCTTGTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
347 CCAAGAGACGG
BslI 311 CCTTGTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
347 CCAAGAGACGG
BsmAI 327 GAGAC GTCTC(1/5)
351 GAGAC
BssKI 27 CCTGG ^CC[ACGT]GG
267 CCTGG
BstNI 27 CCTGG CC^[AT]GG
267 CCTGG
DpnII 24 GATC ^GATC
90 GATC
DsaI 215 CCGCGG C^C[GA][CT]GG
240 CCATGG
EarI 7 GAAGAG CTCTTC(1/4)
66 GAAGAG
EcoRII 27 CCTGG ^CC[AT]GG
267 CCTGG
HinfI 117 GATTC G^A[ACGT]TC
360 GAGTC
HphI 92 TCACC GGTGA(8/7)
212 TCACC
Ksp632I 7 GAAGAG CTCTTC(1/4)
66 GAAGAG
MboI 24 GATC ^GATC
90 GATC
MnlI 103 CCTC CCTC(7/6)
302 CCTC
MspA1I 162 CCGCTG C[AC]G^C[GT]G
215 CCGCGG
MvaI 27 CCTGG CC^[AT]GG
267 CCTGG
NdeII 24 GATC ^GATC
90 GATC
NlaIII 48 CATG CATG^
241 CATG
NlaIV 31 GGAGCC GG[ACGT]^[ACGT]CC
142 GGTACC
SapI 7 GAAGAGC GCTCTTC(1/4)
66 GAAGAGC
Sau3AI 24 GATC ^GATC
90 GATC
ScrFI 27 CCTGG CC^[ACGT]GG
267 CCTGG
SfaNI 167 GCATC GCATC(5/9)
203 GCATC
TspRI 111 CAGTG CA[GC]TG[ACGT][ACGT]^
306 CAGTG
XcmI 95 CCACAGTCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
257 CCATCTTTGGCCTGG
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
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AciI AluI BanII BbvI BmyI
BsaJI Bsp1286I Fnu4HI ItaI MboII
MwoI TseI