(Circular) (Six-base) MAPSORT of: pFN21AB7062.seq Check: 1399 from: 1 to: 6697 pFN21AB7062 6697 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2023 2023 Size: 6697 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4507 278 Size: 53 83 186 936 2971 2468 Fragments arranged by size: 2971 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3166 3387 3771 4040 1937 Size: 1229 221 384 269 4594 Fragments arranged by size: 4594 1229 384 269 221 Acc65I G'GTAC_C Cuts at: 4018 4018 Size: 6697 AclI AA'CG_TT Cuts at: 1563 4824 5197 1563 Size: 3261 373 3063 Fragments arranged by size: 3261 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2262 2618 2996 3993 4760 6144 6412 1632 Size: 630 356 378 997 767 1384 268 1917 Fragments arranged by size: 1917 1384 997 767 630 378 356 268 AflII C'TTAA_G Cuts at: 820 1017 2773 3058 820 Size: 197 1756 285 4459 Fragments arranged by size: 4459 1756 285 197 AflIII A'CryG_T Cuts at: 1203 2244 2334 2646 3069 5596 6573 1203 Size: 1041 90 312 423 2527 977 1327 Fragments arranged by size: 2527 1327 1041 977 423 312 90 AgeI A'CCGG_T Cuts at: 5528 5528 Size: 6697 AhdI GACnn_n'nnGTC Cuts at: 5426 5426 Size: 6697 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6697 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3261 1184 Size: 2077 4620 AlwNI CAG_nnn'CTG Cuts at: 3122 3971 4440 6012 3122 Size: 849 469 1572 3807 Fragments arranged by size: 3807 1572 849 469 ApaI G_GGCC'C Cuts at: 2022 2022 Size: 6697 ApaLI G'TGCA_C Cuts at: 4754 5910 4754 Size: 1156 5541 ApoI r'AATT_y Cuts at: 1493 2655 3419 3486 3977 4006 4232 4384 6493 Size: 1162 764 67 491 29 226 152 2109 Cuts at: 6493 1493 Size: 1697 Fragments arranged by size: 2109 1697 1162 764 491 226 152 67 29 AseI AT'TA_AT Cuts at: 160 1032 2465 3128 3591 5251 160 Size: 872 1433 663 463 1660 1606 Fragments arranged by size: 1660 1606 1433 872 663 463 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6697 AvaI C'yCGr_G Cuts at: 1870 1943 4022 1870 Size: 73 2079 4545 Fragments arranged by size: 4545 2079 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3981 4014 3981 Size: 33 6664 BamHI G'GATC_C Cuts at: 4027 4394 4027 Size: 367 6330 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2086 2324 4018 5473 618 Size: 325 217 627 299 238 1694 1455 1842 Fragments arranged by size: 1842 1694 1455 627 325 299 238 217 BanII G_rGCy'C Cuts at: 1391 2022 4016 1391 Size: 631 1994 4072 Fragments arranged by size: 4072 1994 631 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3560 928 Size: 971 1661 4065 Fragments arranged by size: 4065 1661 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2633 2667 4888 4922 1903 Size: 34 696 34 2221 34 3678 Fragments arranged by size: 3678 2221 696 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2417 4589 5799 2417 Size: 2172 1210 3315 Fragments arranged by size: 3315 2172 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6697 BfrBI ATG'CAT Cuts at: 3515 3515 Size: 6697 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5308 136 Size: 107 122 71 631 605 337 3299 1525 Fragments arranged by size: 3299 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2166 6692 2166 Size: 4526 2171 BlpI GC'TnA_GC Cuts at: 2254 4114 2254 Size: 1860 4837 Bme1580I G_kGCm'C Cuts at: 1790 2022 2091 4758 5914 1790 Size: 232 69 2667 1156 2573 Fragments arranged by size: 2667 2573 1156 232 69 BmgBI CAC'GTC Cuts at: 1318 2337 1318 Size: 1019 5678 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2888 3027 4428 Size: 416 53 83 399 24 1471 139 1401 Cuts at: 4428 5386 442 Size: 958 1753 Fragments arranged by size: 1753 1471 1401 958 416 399 139 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6697 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2423 3828 5357 1239 Size: 908 276 1405 1529 2579 Fragments arranged by size: 2579 1529 1405 908 276 Bpu10I CC'TnA_GC Cuts at: 2748 2748 Size: 6697 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4822 5687 5985 4822 Size: 865 298 5534 Fragments arranged by size: 5534 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5360 882 Size: 617 3861 2219 Fragments arranged by size: 3861 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2247 4488 493 Size: 711 1043 2241 2702 Fragments arranged by size: 2702 2241 1043 711 BsaBI GATnn'nnATC Cuts at: 6691 6691 Size: 6697 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2109 2968 4504 4886 Size: 53 83 186 936 576 859 1536 382 Cuts at: 4886 5521 275 Size: 635 1451 Fragments arranged by size: 1536 1451 936 859 635 576 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5130 5528 5802 5949 6360 5130 Size: 398 274 147 411 5467 Fragments arranged by size: 5467 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6472 Fragments arranged by size: 6472 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6697 BseYI C'CCAG_C Cuts at: 5900 6396 5900 Size: 496 6201 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6124 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 4147 4908 5936 1540 Size: 275 186 12 2134 761 1028 2301 Fragments arranged by size: 2301 2134 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2163 4758 4843 5914 2163 Size: 2595 85 1071 2946 Fragments arranged by size: 2946 2595 1071 85 BsmI GAATG_Cn' Cuts at: 4208 4301 4208 Size: 93 6604 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2091 2163 4016 4758 4843 5914 Size: 399 232 69 72 1853 742 85 1071 Cuts at: 5914 1391 Size: 2174 Fragments arranged by size: 2174 1853 1071 742 399 232 85 72 69 BspEI T'CCGG_A Cuts at: 6360 6360 Size: 6697 BspHI T'CATG_A Cuts at: 3336 3658 4586 6316 3336 Size: 322 928 1730 3717 Fragments arranged by size: 3717 1730 928 322 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2023 2044 4052 844 Size: 1179 21 2008 3489 Fragments arranged by size: 3489 2008 1179 21 BsrBI CCG'CTC Cuts at: 1394 1823 4584 1394 Size: 429 2761 3507 Fragments arranged by size: 3507 2761 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5192 5366 65 Size: 1183 3944 174 1396 Fragments arranged by size: 3944 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2145 5341 5528 1700 Size: 110 335 3196 187 2869 Fragments arranged by size: 3196 2869 335 187 110 BsrGI T'GTAC_A Cuts at: 96 2766 3732 96 Size: 2670 966 3061 Fragments arranged by size: 3061 2670 966 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6697 BssSI C'ACGA_G Cuts at: 4757 5769 4757 Size: 1012 5685 BstAPI GCAn_nnn'nTGC Cuts at: 794 2035 794 Size: 1241 5456 BstBI TT'CG_AA Cuts at: 5501 5501 Size: 6697 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6697 BstYI r'GATC_y Cuts at: 1972 2166 2476 4027 4394 4779 4796 6248 6334 Size: 194 310 1551 367 385 17 1452 86 Cuts at: 6334 6346 6692 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1551 1452 385 367 346 310 194 86 17 12 BstZ17I GTA'TAC Cuts at: 3772 3772 Size: 6697 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 3106 513 Size: 552 939 9 1093 4104 Fragments arranged by size: 4104 1093 939 552 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5958 Fragments arranged by size: 5958 683 56 BtsI GCAGTG_nn' Cuts at: 2595 4213 5007 5027 2595 Size: 1618 794 20 4265 Fragments arranged by size: 4265 1618 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6697 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6662 DraI TTT'AAA Cuts at: 4001 4001 Size: 6697 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3383 5704 809 Size: 507 2067 2321 1802 Fragments arranged by size: 2321 2067 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 4144 5033 Size: 54 1425 50 275 18 180 2134 889 Cuts at: 5033 6662 8 Size: 1629 43 Fragments arranged by size: 2134 1629 1425 889 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2010 4144 1537 Size: 275 198 2134 4090 Fragments arranged by size: 4090 2134 275 198 EarI CTCTTCn'nnn_ Cuts at: 1353 2687 3015 3036 4627 1353 Size: 1334 328 21 1591 3423 Fragments arranged by size: 3423 1591 1334 328 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5284 5658 5804 387 Size: 1064 138 3695 374 146 1280 Fragments arranged by size: 3695 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2262 2423 2618 2996 3828 3993 Size: 393 515 115 161 195 378 832 165 Cuts at: 3993 4760 5357 6144 6412 1239 Size: 767 597 787 268 1524 Fragments arranged by size: 1524 832 787 767 597 515 393 378 268 195 165 161 115 EcoNI CCTnn'n_nnAGG Cuts at: 2367 2493 2367 Size: 126 6571 EcoO109I rG'GnC_Cy Cuts at: 2018 2019 2372 2827 2018 Size: 1 353 455 5888 Fragments arranged by size: 5888 455 353 1 EcoRI G'AATT_C Cuts at: 4006 4384 4006 Size: 378 6319 EcoRV GAT'ATC Cuts at: 3787 3787 Size: 6697 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3065 3065 Size: 6697 FspI TGC'GCA Cuts at: 5203 5203 Size: 6697 HaeII r_GCGC'y Cuts at: 1678 2069 2081 5844 1678 Size: 391 12 3763 2531 Fragments arranged by size: 3763 2531 391 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2032 2064 5338 5370 5412 Size: 32 135 32 460 32 3274 32 42 Cuts at: 5412 5444 1373 Size: 32 2626 Fragments arranged by size: 3274 2626 460 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4041 4287 669 Size: 988 281 2103 246 3079 Fragments arranged by size: 3079 2103 988 281 246 HindIII A'AGCT_T Cuts at: 748 3371 748 Size: 2623 4074 HpaI GTT'AAC Cuts at: 4287 4287 Size: 6697 KpnI G_GTAC'C Cuts at: 4022 4022 Size: 6697 MfeI C'AATT_G Cuts at: 4296 4296 Size: 6697 MluI A'CGCG_T Cuts at: 6573 6573 Size: 6697 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5811 5995 1305 Size: 4506 184 2007 Fragments arranged by size: 4506 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4875 Fragments arranged by size: 4875 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3243 3464 4655 5014 5173 518 Size: 648 2077 221 1191 359 159 2042 Fragments arranged by size: 2077 2042 1191 648 359 221 159 MspA1I CmG'CkG Cuts at: 1606 3971 4113 4790 5938 6183 1606 Size: 2365 142 677 1148 245 2120 Fragments arranged by size: 2365 2120 1148 677 245 142 NcoI C'CATG_G Cuts at: 513 1065 2004 3106 513 Size: 552 939 1102 4104 Fragments arranged by size: 4104 1102 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6697 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6697 NotI GC'GGCC_GC Cuts at: 4144 4144 Size: 6697 NsiI A_TGCA'T Cuts at: 3517 3517 Size: 6697 NspI r_CATG'y Cuts at: 1132 1229 2650 3073 3515 4055 5600 1132 Size: 97 1421 423 442 540 1545 2229 Fragments arranged by size: 2229 1545 1421 540 442 423 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6697 PciI A'CATG_T Cuts at: 2646 3069 5596 2646 Size: 423 2527 3747 Fragments arranged by size: 3747 2527 423 PflMI CCAn_nnn'nTGG Cuts at: 1108 2917 1108 Size: 1809 4888 PmeI GTTT'AAAC Cuts at: 4001 4001 Size: 6697 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4766 4798 6279 6311 4766 Size: 32 1481 32 5152 Fragments arranged by size: 5152 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2372 2827 2372 Size: 455 6242 PshAI GACnn'nnGTC Cuts at: 1613 2967 1613 Size: 1354 5343 PsiI TTA'TAA Cuts at: 2846 4267 2846 Size: 1421 5276 PspOMI G'GGCC_C Cuts at: 2018 2018 Size: 6697 PstI C_TGCA'G Cuts at: 830 3091 4049 830 Size: 2261 958 3478 Fragments arranged by size: 3478 2261 958 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6697 PvuII CAG'CTG Cuts at: 3971 3971 Size: 6697 SalI G'TCGA_C Cuts at: 1936 4039 1936 Size: 2103 4594 SapI GCTCTTCn'nnn_ Cuts at: 3015 3015 Size: 6697 SbfI CC_TGCA'GG Cuts at: 4049 4049 Size: 6697 ScaI AGT'ACT Cuts at: 4945 6657 4945 Size: 1712 4985 SfcI C'TryA_G Cuts at: 826 1045 2402 2754 3087 4045 5180 5861 6052 Size: 219 1357 352 333 958 1135 681 191 Cuts at: 6052 826 Size: 1471 Fragments arranged by size: 1471 1357 1135 958 681 352 333 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6697 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6697 SmaI CCC'GGG Cuts at: 1872 4024 1872 Size: 2152 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2773 3058 4801 5702 5964 820 Size: 197 926 830 285 1743 901 262 1553 Fragments arranged by size: 1743 1553 926 901 830 285 262 197 SnaBI TAC'GTA Cuts at: 493 4488 493 Size: 3995 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6697 SphI G_CATG'C Cuts at: 1132 4055 1132 Size: 2923 3774 SspI AAT'ATT Cuts at: 5 52 2931 4621 5 Size: 47 2879 1690 2081 Fragments arranged by size: 2879 2081 1690 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6697 StyI C'CwwG_G Cuts at: 513 1065 2004 3106 4136 513 Size: 552 939 1102 1030 3074 Fragments arranged by size: 3074 1102 1030 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2844 3847 4724 4741 4894 5079 5524 Size: 1255 1264 1003 877 17 153 185 445 Cuts at: 5524 325 Size: 1498 Fragments arranged by size: 1498 1264 1255 1003 877 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2766 2875 3732 Size: 274 80 33 51 1443 789 109 857 Cuts at: 3732 3905 4943 6655 96 Size: 173 1038 1712 138 Fragments arranged by size: 1712 1443 1038 857 789 274 173 138 109 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6657 6689 6657 Size: 32 6665 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6697 XbaI T'CTAG_A Cuts at: 4033 4033 Size: 6697 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6697 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6697 XmaI C'CCGG_G Cuts at: 1870 4022 1870 Size: 2152 4545 XmnI GAAnn'nnTTC Cuts at: 4826 4826 Size: 6697 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4505 276 Size: 53 83 186 936 2971 2468 Fragments arranged by size: 2971 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI