(Circular) (Six-base) MAPSORT of: pFN21AB7058.seq Check: 4576 from: 1 to: 6544 pFN21AB7058 6544 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3392 3392 Size: 6544 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4354 278 Size: 53 83 186 936 2818 2468 Fragments arranged by size: 2818 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3887 1937 Size: 1950 4594 Acc65I G'GTAC_C Cuts at: 3865 3865 Size: 6544 AclI AA'CG_TT Cuts at: 1563 4671 5044 1563 Size: 3108 373 3063 Fragments arranged by size: 3108 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2426 2765 3655 4607 5991 6259 1632 Size: 794 339 890 952 1384 268 1917 Fragments arranged by size: 1917 1384 952 890 794 339 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6347 AflIII A'CryG_T Cuts at: 1203 5443 6420 1203 Size: 4240 977 1327 Fragments arranged by size: 4240 1327 977 AgeI A'CCGG_T Cuts at: 5375 5375 Size: 6544 AhdI GACnn_n'nnGTC Cuts at: 5273 5273 Size: 6544 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6544 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3029 3274 1184 Size: 1845 245 4454 Fragments arranged by size: 4454 1845 245 AlwNI CAG_nnn'CTG Cuts at: 4287 5859 4287 Size: 1572 4972 ApaLI G'TGCA_C Cuts at: 2136 4601 5757 2136 Size: 2465 1156 2923 Fragments arranged by size: 2923 2465 1156 ApoI r'AATT_y Cuts at: 1493 2185 3853 4079 4231 6340 1493 Size: 692 1668 226 152 2109 1697 Fragments arranged by size: 2109 1697 1668 692 226 152 AseI AT'TA_AT Cuts at: 160 1032 5098 160 Size: 872 4066 1606 Fragments arranged by size: 4066 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6544 AvaI C'yCGr_G Cuts at: 1870 1943 2014 2087 2211 2278 2332 2379 2647 Size: 73 71 73 124 67 54 47 268 Cuts at: 2647 3435 3869 1870 Size: 788 434 4545 Fragments arranged by size: 4545 788 434 268 124 73 73 71 67 54 47 BamHI G'GATC_C Cuts at: 2672 3874 4241 2672 Size: 1202 367 4975 Fragments arranged by size: 4975 1202 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3865 5320 618 Size: 325 217 627 2078 1455 1842 Fragments arranged by size: 2078 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2014 2212 3175 3247 3863 1391 Size: 623 198 963 72 616 4072 Fragments arranged by size: 4072 963 623 616 198 72 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2522 3720 928 Size: 971 623 1198 3752 Fragments arranged by size: 3752 1198 971 623 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4735 4769 1903 Size: 34 50 34 2714 34 3678 Fragments arranged by size: 3678 2714 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4436 5646 4436 Size: 1210 5334 BclI T'GATC_A Cuts at: 1548 1548 Size: 6544 BfrBI ATG'CAT Cuts at: 3717 3717 Size: 6544 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2912 3557 5155 Size: 107 122 71 631 605 1240 645 1598 Cuts at: 5155 136 Size: 1525 Fragments arranged by size: 1598 1525 1240 645 631 605 122 107 71 BglII A'GATC_T Cuts at: 2445 3270 6539 2445 Size: 825 3269 2450 Fragments arranged by size: 3269 2450 825 BlpI GC'TnA_GC Cuts at: 3212 3961 3212 Size: 749 5795 Bme1580I G_kGCm'C Cuts at: 1790 2140 2625 3018 4605 5761 1790 Size: 350 485 393 1587 1156 2573 Fragments arranged by size: 2573 1587 1156 485 393 350 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6544 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3635 4275 5233 Size: 416 53 83 399 24 2218 640 958 Cuts at: 5233 442 Size: 1753 Fragments arranged by size: 2218 1753 958 640 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6544 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3016 3016 Size: 6544 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2383 2414 2555 3011 5204 1239 Size: 1144 31 141 456 2193 2579 Fragments arranged by size: 2579 2193 1144 456 141 31 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2048 4669 5534 5832 2048 Size: 2621 865 298 2760 Fragments arranged by size: 2760 2621 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5207 882 Size: 617 3708 2219 Fragments arranged by size: 3708 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4335 493 Size: 711 3131 2702 Fragments arranged by size: 3131 2702 711 BsaBI GATnn'nnATC Cuts at: 2855 6538 2855 Size: 3683 2861 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2169 2315 4351 4733 Size: 53 83 186 936 636 146 2036 382 Cuts at: 4733 5368 275 Size: 635 1451 Fragments arranged by size: 2036 1451 936 636 635 382 186 146 83 53 BsaWI w'CCGG_w Cuts at: 4977 5375 5649 5796 6207 4977 Size: 398 274 147 411 5314 Fragments arranged by size: 5314 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6319 Fragments arranged by size: 6319 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2240 2468 1186 Size: 1054 228 5262 Fragments arranged by size: 5262 1054 228 BseYI C'CCAG_C Cuts at: 5747 6243 5747 Size: 496 6048 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5971 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3994 4755 5783 1540 Size: 275 186 1993 761 1028 2301 Fragments arranged by size: 2301 1993 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2140 2218 2380 3067 3175 3247 4605 4690 5761 Size: 78 162 687 108 72 1358 85 1071 Cuts at: 5761 2140 Size: 2923 Fragments arranged by size: 2923 1358 1071 687 162 108 85 78 72 BsmI GAATG_Cn' Cuts at: 3717 3719 4055 4148 3717 Size: 2 336 93 6113 Fragments arranged by size: 6113 336 93 2 BsmBI CGTCTCn'nnnn_ Cuts at: 2190 2190 Size: 6544 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2140 2212 2218 2380 2625 3018 Size: 399 224 126 72 6 162 245 393 Cuts at: 3018 3067 3175 3247 3863 4605 4690 5761 1391 Size: 49 108 72 616 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 616 399 393 245 224 162 126 108 85 72 72 49 6 BspEI T'CCGG_A Cuts at: 6207 6207 Size: 6544 BspHI T'CATG_A Cuts at: 4433 6163 4433 Size: 1730 4814 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2274 3392 3899 844 Size: 1430 1118 507 3489 Fragments arranged by size: 3489 1430 1118 507 BsrBI CCG'CTC Cuts at: 1394 1823 4431 1394 Size: 429 2608 3507 Fragments arranged by size: 3507 2608 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3838 5039 5213 65 Size: 1183 2590 1201 174 1396 Fragments arranged by size: 2590 1396 1201 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2269 2730 2825 5188 5375 1700 Size: 110 459 461 95 2363 187 2869 Fragments arranged by size: 2869 2363 461 459 187 110 95 BsrGI T'GTAC_A Cuts at: 96 3479 96 Size: 3383 3161 BssHII G'CGCG_C Cuts at: 1925 2019 1925 Size: 94 6450 BssSI C'ACGA_G Cuts at: 2966 4604 5616 2966 Size: 1638 1012 3894 Fragments arranged by size: 3894 1638 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2862 794 Size: 2068 4476 BstBI TT'CG_AA Cuts at: 5348 5348 Size: 6544 BstEII G'GTnAC_C Cuts at: 1184 3736 1184 Size: 2552 3992 BstXI CCAn_nnnn'nTGG Cuts at: 2614 2699 2614 Size: 85 6459 BstYI r'GATC_y Cuts at: 1972 2445 2672 3270 3874 4241 4626 4643 6095 Size: 473 227 598 604 367 385 17 1452 Cuts at: 6095 6181 6193 6539 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 604 598 473 385 367 346 227 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2158 2421 2808 3768 513 Size: 552 1093 263 387 960 3289 Fragments arranged by size: 3289 1093 960 552 387 263 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5805 Fragments arranged by size: 5805 683 56 BtsI GCAGTG_nn' Cuts at: 4060 4854 4874 4060 Size: 794 20 5730 Fragments arranged by size: 5730 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6544 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6509 DraI TTT'AAA Cuts at: 3848 3848 Size: 6544 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2773 5551 809 Size: 507 1457 2778 1802 Fragments arranged by size: 2778 1802 1457 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2549 2604 2904 Size: 54 1425 50 275 18 719 55 300 Cuts at: 2904 3238 3991 4880 6509 8 Size: 334 753 889 1629 43 Fragments arranged by size: 1629 1425 889 753 719 334 300 275 55 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3991 1537 Size: 275 2179 4090 Fragments arranged by size: 4090 2179 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2083 2441 2454 4474 1353 Size: 730 358 13 2020 3423 Fragments arranged by size: 3423 2020 730 358 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2324 3194 5131 5505 5651 387 Size: 1064 138 735 870 1937 374 146 1280 Fragments arranged by size: 1937 1280 1064 870 735 374 146 138 EcoICRI GAG'CTC Cuts at: 3173 3245 3173 Size: 72 6472 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2383 2414 2426 2555 2765 3011 3655 Size: 393 751 31 12 129 210 246 644 Cuts at: 3655 4607 5204 5991 6259 1239 Size: 952 597 787 268 1524 Fragments arranged by size: 1524 952 787 751 644 597 393 268 246 210 129 31 12 EcoO109I rG'GnC_Cy Cuts at: 2252 2263 2336 2468 2705 2252 Size: 11 73 132 237 6091 Fragments arranged by size: 6091 237 132 73 11 EcoRI G'AATT_C Cuts at: 3853 4231 3853 Size: 378 6166 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2770 2770 Size: 6544 FspI TGC'GCA Cuts at: 5050 5050 Size: 6544 HaeII r_GCGC'y Cuts at: 1678 2158 2481 2660 5691 1678 Size: 480 323 179 3031 2531 Fragments arranged by size: 3031 2531 480 323 179 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5185 5217 5259 5291 1373 Size: 32 135 32 3613 32 42 32 2626 Fragments arranged by size: 3613 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3888 4134 669 Size: 988 281 1950 246 3079 Fragments arranged by size: 3079 1950 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6544 HpaI GTT'AAC Cuts at: 4134 4134 Size: 6544 KpnI G_GTAC'C Cuts at: 3869 3869 Size: 6544 MfeI C'AATT_G Cuts at: 2637 4143 2637 Size: 1506 5038 MluI A'CGCG_T Cuts at: 6420 6420 Size: 6544 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2366 2916 3811 5658 5842 1305 Size: 1061 550 895 1847 184 2007 Fragments arranged by size: 2007 1847 1061 895 550 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2551 2906 10 Size: 54 1425 343 719 355 3648 Fragments arranged by size: 3648 1425 719 355 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4502 4861 5020 518 Size: 648 3336 359 159 2042 Fragments arranged by size: 3336 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2160 2764 2953 3211 3960 4637 5785 6030 Size: 554 604 189 258 749 677 1148 245 Cuts at: 6030 1606 Size: 2120 Fragments arranged by size: 2120 1148 749 677 604 554 258 245 189 NaeI GCC'GGC Cuts at: 2271 2271 Size: 6544 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5992 NdeI CA'TA_TG Cuts at: 387 387 Size: 6544 NgoMIV G'CCGG_C Cuts at: 2269 2269 Size: 6544 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6544 NotI GC'GGCC_GC Cuts at: 3991 3991 Size: 6544 NsiI A_TGCA'T Cuts at: 3719 3719 Size: 6544 NspI r_CATG'y Cuts at: 1132 1229 2038 2881 3902 5447 1132 Size: 97 809 843 1021 1545 2229 Fragments arranged by size: 2229 1545 1021 843 809 97 PasI CC'CwG_GG Cuts at: 1348 2220 1348 Size: 872 5672 PciI A'CATG_T Cuts at: 5443 5443 Size: 6544 PflMI CCAn_nnn'nTGG Cuts at: 1108 2982 1108 Size: 1874 4670 PmeI GTTT'AAAC Cuts at: 3848 3848 Size: 6544 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3765 3797 4613 4645 6126 6158 3765 Size: 32 816 32 1481 32 4151 Fragments arranged by size: 4151 1481 816 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2252 2263 2336 2468 2705 2252 Size: 11 73 132 237 6091 Fragments arranged by size: 6091 237 132 73 11 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6544 PsiI TTA'TAA Cuts at: 4114 4114 Size: 6544 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2741 2773 2741 Size: 32 6512 PstI C_TGCA'G Cuts at: 830 2299 3896 830 Size: 1469 1597 3478 Fragments arranged by size: 3478 1597 1469 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6544 PvuII CAG'CTG Cuts at: 3211 3211 Size: 6544 SacI G_AGCT'C Cuts at: 3175 3247 3175 Size: 72 6472 SacII CC_GC'GG Cuts at: 2161 2161 Size: 6544 SalI G'TCGA_C Cuts at: 1936 3886 1936 Size: 1950 4594 SanDI GG'GwC_CC Cuts at: 2252 2468 2705 2252 Size: 216 237 6091 Fragments arranged by size: 6091 237 216 SapI GCTCTTCn'nnn_ Cuts at: 2441 2441 Size: 6544 SbfI CC_TGCA'GG Cuts at: 3896 3896 Size: 6544 ScaI AGT'ACT Cuts at: 4792 6504 4792 Size: 1712 4832 SfcI C'TryA_G Cuts at: 826 1045 2142 2295 3561 3892 5027 5708 5899 Size: 219 1097 153 1266 331 1135 681 191 Cuts at: 5899 826 Size: 1471 Fragments arranged by size: 1471 1266 1135 1097 681 331 219 191 153 SfiI GGCCn_nnn'nGGCC Cuts at: 2912 2912 Size: 6544 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6544 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6544 SmaI CCC'GGG Cuts at: 1872 2016 2280 2334 2649 3871 1872 Size: 144 264 54 315 1222 4545 Fragments arranged by size: 4545 1222 315 264 144 54 SmlI C'TyrA_G Cuts at: 820 1017 1943 2063 4648 5549 5811 820 Size: 197 926 120 2585 901 262 1553 Fragments arranged by size: 2585 1553 926 901 262 197 120 SnaBI TAC'GTA Cuts at: 493 4335 493 Size: 3842 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6544 SphI G_CATG'C Cuts at: 1132 3902 1132 Size: 2770 3774 SspI AAT'ATT Cuts at: 5 52 4468 5 Size: 47 4416 2081 Fragments arranged by size: 4416 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6544 StyI C'CwwG_G Cuts at: 513 1065 2552 2676 3983 513 Size: 552 1487 124 1307 3074 Fragments arranged by size: 3074 1487 1307 552 124 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2233 2669 3154 4571 4588 4741 4926 Size: 1255 653 436 485 1417 17 153 185 Cuts at: 4926 5371 325 Size: 445 1498 Fragments arranged by size: 1498 1417 1255 653 485 445 436 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3108 3479 3762 Size: 274 80 33 51 1443 1131 371 283 Cuts at: 3762 4790 6502 96 Size: 1028 1712 138 Fragments arranged by size: 1712 1443 1131 1028 371 283 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6504 6536 6504 Size: 32 6512 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6544 XbaI T'CTAG_A Cuts at: 3880 3880 Size: 6544 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2358 2777 2899 2358 Size: 419 122 6003 Fragments arranged by size: 6003 419 122 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6544 XmaI C'CCGG_G Cuts at: 1870 2014 2278 2332 2647 3869 1870 Size: 144 264 54 315 1222 4545 Fragments arranged by size: 4545 1222 315 264 144 54 XmnI GAAnn'nnTTC Cuts at: 2753 3198 3717 4673 2753 Size: 445 519 956 4624 Fragments arranged by size: 4624 956 519 445 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4352 276 Size: 53 83 186 936 2818 2468 Fragments arranged by size: 2818 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI RsrII SexAI SfoI SrfI SwaI