(Circular) (Six-base) MAPSORT of: pFN21AB7057.seq Check: 967 from: 1 to: 6520 pFN21AB7057 6520 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2391 3133 3357 2391 Size: 742 224 5554 Fragments arranged by size: 5554 742 224 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3471 4330 278 Size: 53 83 186 936 1935 859 2468 Fragments arranged by size: 2468 1935 936 859 186 83 53 AccI GT'mk_AC Cuts at: 1937 3863 1937 Size: 1926 4594 Acc65I G'GTAC_C Cuts at: 3841 3841 Size: 6520 AclI AA'CG_TT Cuts at: 1563 4647 5020 1563 Size: 3084 373 3063 Fragments arranged by size: 3084 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2629 2819 4583 5967 6235 1632 Size: 997 190 1764 1384 268 1917 Fragments arranged by size: 1917 1764 1384 997 268 190 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6323 AflIII A'CryG_T Cuts at: 1203 2712 2856 5419 6396 1203 Size: 1509 144 2563 977 1327 Fragments arranged by size: 2563 1509 1327 977 144 AgeI A'CCGG_T Cuts at: 5351 5351 Size: 6520 AhdI GACnn_n'nnGTC Cuts at: 5249 5249 Size: 6520 AleI CACnn'nnGTG Cuts at: 1166 3092 1166 Size: 1926 4594 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6520 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2843 2875 3131 3163 2843 Size: 32 256 32 6200 Fragments arranged by size: 6200 256 32 32 AlwNI CAG_nnn'CTG Cuts at: 2356 2520 3292 3373 4263 5835 2356 Size: 164 772 81 890 1572 3041 Fragments arranged by size: 3041 1572 890 772 164 81 ApaI G_GGCC'C Cuts at: 2069 2120 3443 2069 Size: 51 1323 5146 Fragments arranged by size: 5146 1323 51 ApaLI G'TGCA_C Cuts at: 4577 5733 4577 Size: 1156 5364 ApoI r'AATT_y Cuts at: 1493 3829 4055 4207 6316 1493 Size: 2336 226 152 2109 1697 Fragments arranged by size: 2336 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5074 160 Size: 872 4042 1606 Fragments arranged by size: 4042 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 AvaI C'yCGr_G Cuts at: 1870 1943 2221 2779 3232 3580 3764 3845 1870 Size: 73 278 558 453 348 184 81 4545 Fragments arranged by size: 4545 558 453 348 278 184 81 73 BamHI G'GATC_C Cuts at: 3850 4217 3850 Size: 367 6153 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2228 2783 3511 3841 5296 Size: 325 217 627 441 555 728 330 1455 Cuts at: 5296 618 Size: 1842 Fragments arranged by size: 1842 1455 728 627 555 441 330 325 217 BanII G_rGCy'C Cuts at: 1391 2069 2120 2172 2499 2664 3240 3321 3443 Size: 678 51 52 327 165 576 81 122 Cuts at: 3443 3780 3839 1391 Size: 337 59 4072 Fragments arranged by size: 4072 678 576 337 327 165 122 81 59 52 51 BbeI G_GCGC'C Cuts at: 2787 2787 Size: 6520 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2170 928 Size: 971 271 5278 Fragments arranged by size: 5278 971 271 BbvCI CC'TCA_GC Cuts at: 2462 3536 3794 2462 Size: 1074 258 5188 Fragments arranged by size: 5188 1074 258 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2746 2780 4711 4745 1903 Size: 34 809 34 1931 34 3678 Fragments arranged by size: 3678 1931 809 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4412 5622 4412 Size: 1210 5310 BclI T'GATC_A Cuts at: 1548 1548 Size: 6520 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2334 2705 3273 Size: 107 122 71 631 605 662 371 568 Cuts at: 3273 5131 136 Size: 1858 1525 Fragments arranged by size: 1858 1525 662 631 605 568 371 122 107 71 BglII A'GATC_T Cuts at: 6515 6515 Size: 6520 BlpI GC'TnA_GC Cuts at: 2171 2994 3937 2171 Size: 823 943 4754 Fragments arranged by size: 4754 943 823 Bme1580I G_kGCm'C Cuts at: 1790 2069 2120 2129 3443 3681 4581 5737 1790 Size: 279 51 9 1314 238 900 1156 2573 Fragments arranged by size: 2573 1314 1156 900 279 238 51 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6520 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2072 3527 3663 Size: 416 53 83 399 24 655 1455 136 Cuts at: 3663 4251 5209 442 Size: 588 958 1753 Fragments arranged by size: 1753 1455 958 655 588 416 399 136 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6520 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2173 2281 2363 2411 2507 2720 2870 Size: 860 74 108 82 48 96 213 150 Cuts at: 2870 2972 3038 3203 3335 3404 3506 3608 3623 Size: 102 66 165 132 69 102 102 15 Cuts at: 3623 5180 1239 Size: 1557 2579 Fragments arranged by size: 2579 1557 860 213 165 150 132 108 102 102 102 96 82 74 69 66 48 15 Bpu10I CC'TnA_GC Cuts at: 2462 2678 3536 3794 2462 Size: 216 858 258 5188 Fragments arranged by size: 5188 858 258 216 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3044 3239 3620 4645 5510 5808 3044 Size: 195 381 1025 865 298 3756 Fragments arranged by size: 3756 1025 865 381 298 195 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5183 882 Size: 617 3684 2219 Fragments arranged by size: 3684 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4311 493 Size: 711 3107 2702 Fragments arranged by size: 3107 2702 711 BsaBI GATnn'nnATC Cuts at: 6514 6514 Size: 6520 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2784 3432 3468 4327 Size: 53 83 186 936 1251 648 36 859 Cuts at: 4327 4709 5344 275 Size: 382 635 1451 Fragments arranged by size: 1451 1251 936 859 648 635 382 186 83 53 36 BsaWI w'CCGG_w Cuts at: 3126 3136 4953 5351 5625 5772 6183 3126 Size: 10 1817 398 274 147 411 3463 Fragments arranged by size: 3463 1817 411 398 274 147 10 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2843 2873 3131 3161 3314 Size: 30 165 30 1440 30 258 30 153 Cuts at: 3314 3344 3581 3611 1178 Size: 30 237 30 4087 Fragments arranged by size: 4087 1440 258 237 165 153 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2969 3296 1186 Size: 1783 327 4410 Fragments arranged by size: 4410 1783 327 BseYI C'CCAG_C Cuts at: 2119 2332 2359 2872 2965 5723 6219 2119 Size: 213 27 513 93 2758 496 2420 Fragments arranged by size: 2758 2420 513 496 213 93 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2369 2741 2927 3152 3161 1163 Size: 573 633 372 186 225 9 4522 Fragments arranged by size: 4522 633 573 372 225 186 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2133 3970 4731 5759 1540 Size: 275 186 132 1837 761 1028 2301 Fragments arranged by size: 2301 1837 1028 761 275 186 132 BsiHKAI G_wGCw'C Cuts at: 2172 2559 2664 3033 3321 4581 4666 5737 2172 Size: 387 105 369 288 1260 85 1071 2955 Fragments arranged by size: 2955 1260 1071 387 369 288 105 85 BsmI GAATG_Cn' Cuts at: 4031 4124 4031 Size: 93 6427 BsmBI CGTCTCn'nnnn_ Cuts at: 3475 3475 Size: 6520 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2120 2129 2172 2499 2559 2664 Size: 399 279 51 9 43 327 60 105 Cuts at: 2664 3033 3240 3321 3443 3681 3780 3839 4581 Size: 369 207 81 122 238 99 59 742 Cuts at: 4581 4666 5737 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 369 327 279 238 207 122 105 99 85 81 60 59 51 43 9 BspEI T'CCGG_A Cuts at: 3126 3136 6183 3126 Size: 10 3047 3463 Fragments arranged by size: 3463 3047 10 BspHI T'CATG_A Cuts at: 3102 4409 6139 3102 Size: 1307 1730 3483 Fragments arranged by size: 3483 1730 1307 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2325 2391 2637 2949 3133 3357 3399 3792 Size: 1481 66 246 312 184 224 42 393 Cuts at: 3792 3875 844 Size: 83 3489 Fragments arranged by size: 3489 1481 393 312 246 224 184 83 66 42 BsrBI CCG'CTC Cuts at: 1394 1823 2395 4407 1394 Size: 429 572 2012 3507 Fragments arranged by size: 3507 2012 572 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3491 5015 5189 65 Size: 1183 2243 1524 174 1396 Fragments arranged by size: 2243 1524 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3224 3424 5164 5351 1700 Size: 110 1414 200 1740 187 2869 Fragments arranged by size: 2869 1740 1414 200 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6520 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6520 BssSI C'ACGA_G Cuts at: 3269 4580 5592 3269 Size: 1311 1012 4197 Fragments arranged by size: 4197 1311 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3175 794 Size: 2381 4139 BstBI TT'CG_AA Cuts at: 5324 5324 Size: 6520 BstEII G'GTnAC_C Cuts at: 1184 3608 1184 Size: 2424 4096 BstYI r'GATC_y Cuts at: 1972 3850 4217 4602 4619 6071 6157 6169 6515 Size: 1878 367 385 17 1452 86 12 346 Cuts at: 6515 1972 Size: 1977 Fragments arranged by size: 1977 1878 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2012 3542 2012 Size: 1530 4990 BtgI C'CryG_G Cuts at: 513 1065 2004 2575 2772 513 Size: 552 939 571 197 4261 Fragments arranged by size: 4261 939 571 552 197 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2749 2824 2893 487 Size: 683 56 1523 75 69 4114 Fragments arranged by size: 4114 1523 683 75 69 56 BtsI GCAGTG_nn' Cuts at: 3554 4036 4830 4850 3554 Size: 482 794 20 5224 Fragments arranged by size: 5224 794 482 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6520 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2222 2257 529 Size: 35 1658 35 4792 Fragments arranged by size: 4792 1658 35 35 DraI TTT'AAA Cuts at: 3824 3824 Size: 6520 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5527 809 Size: 507 4211 1802 Fragments arranged by size: 4211 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 2326 2655 Size: 54 1425 50 275 18 300 196 329 Cuts at: 2655 3967 4856 6485 8 Size: 1312 889 1629 43 Fragments arranged by size: 1629 1425 1312 889 329 300 275 196 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2130 3967 1537 Size: 275 318 1837 4090 Fragments arranged by size: 4090 1837 318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2504 2648 3254 3368 4450 1353 Size: 1151 144 606 114 1082 3423 Fragments arranged by size: 3423 1151 1082 606 144 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3197 3230 3320 3443 5107 5481 Size: 1064 138 1608 33 90 123 1664 374 Cuts at: 5481 5627 387 Size: 146 1280 Fragments arranged by size: 1664 1608 1280 1064 374 146 138 123 90 33 EcoICRI GAG'CTC Cuts at: 2170 2662 3319 2170 Size: 492 657 5371 Fragments arranged by size: 5371 657 492 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2173 2281 2363 2411 2507 2629 Size: 393 467 74 108 82 48 96 122 Cuts at: 2629 2720 2819 2870 2972 3038 3203 3335 3404 Size: 91 99 51 102 66 165 132 69 Cuts at: 3404 3506 3608 3623 4583 5180 5967 6235 1239 Size: 102 102 15 960 597 787 268 1524 Fragments arranged by size: 1524 960 787 597 467 393 268 165 132 122 108 102 102 102 99 96 91 82 74 69 66 51 48 15 EcoNI CCTnn'n_nnAGG Cuts at: 2849 3365 3797 2849 Size: 516 432 5572 Fragments arranged by size: 5572 516 432 EcoO109I rG'GnC_Cy Cuts at: 2066 2116 2506 2524 2938 3010 3154 3370 3440 Size: 50 390 18 414 72 144 216 70 Cuts at: 3440 3790 2066 Size: 350 4796 Fragments arranged by size: 4796 414 390 350 216 144 72 70 50 18 EcoRI G'AATT_C Cuts at: 3829 4207 3829 Size: 378 6142 FspI TGC'GCA Cuts at: 2408 5026 2408 Size: 2618 3902 HaeII r_GCGC'y Cuts at: 1678 2039 2357 2787 2807 3075 3595 3644 5667 Size: 361 318 430 20 268 520 49 2023 Cuts at: 5667 1678 Size: 2531 Fragments arranged by size: 2531 2023 520 430 361 318 268 49 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3237 3269 3314 3346 5161 Size: 32 135 32 1665 32 45 32 1815 Cuts at: 5161 5193 5235 5267 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1815 1665 135 45 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3864 4110 669 Size: 988 281 1926 246 3079 Fragments arranged by size: 3079 1926 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6520 HpaI GTT'AAC Cuts at: 4110 4110 Size: 6520 KasI G'GCGC_C Cuts at: 2783 2783 Size: 6520 KpnI G_GTAC'C Cuts at: 3845 3845 Size: 6520 MfeI C'AATT_G Cuts at: 4119 4119 Size: 6520 MluI A'CGCG_T Cuts at: 6396 6396 Size: 6520 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3357 5634 5818 1305 Size: 2052 2277 184 2007 Fragments arranged by size: 2277 2052 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4698 Fragments arranged by size: 4698 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3092 4478 4837 4996 518 Size: 648 1926 1386 359 159 2042 Fragments arranged by size: 2042 1926 1386 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2332 2404 2577 2649 2725 2965 2989 3192 Size: 726 72 173 72 76 240 24 203 Cuts at: 3192 3451 3936 4613 5761 6006 1606 Size: 259 485 677 1148 245 2120 Fragments arranged by size: 2120 1148 726 677 485 259 245 240 203 173 76 72 72 24 NaeI GCC'GGC Cuts at: 3426 3426 Size: 6520 NarI GG'CG_CC Cuts at: 2784 2784 Size: 6520 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5029 Fragments arranged by size: 5029 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6520 NgoMIV G'CCGG_C Cuts at: 3424 3424 Size: 6520 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6520 NotI GC'GGCC_GC Cuts at: 3967 3967 Size: 6520 NspI r_CATG'y Cuts at: 1132 1229 3878 5423 1132 Size: 97 2649 1545 2229 Fragments arranged by size: 2649 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3436 3615 1348 Size: 2088 179 4253 Fragments arranged by size: 4253 2088 179 PciI A'CATG_T Cuts at: 5419 5419 Size: 6520 PflMI CCAn_nnn'nTGG Cuts at: 1108 3373 1108 Size: 2265 4255 PfoI T'CCnGG_A Cuts at: 2189 2664 2847 2949 3054 2189 Size: 475 183 102 105 5655 Fragments arranged by size: 5655 475 183 105 102 PmeI GTTT'AAAC Cuts at: 3824 3824 Size: 6520 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2843 2875 3131 3163 4589 4621 6102 6134 2843 Size: 32 256 32 1426 32 1481 32 3229 Fragments arranged by size: 3229 1481 1426 256 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2524 2938 3790 2524 Size: 414 852 5254 Fragments arranged by size: 5254 852 414 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6520 PsiI TTA'TAA Cuts at: 4090 4090 Size: 6520 PspOMI G'GGCC_C Cuts at: 2065 2116 3439 2065 Size: 51 1323 5146 Fragments arranged by size: 5146 1323 51 PstI C_TGCA'G Cuts at: 830 2147 2186 2322 2634 2964 3450 3663 3872 Size: 1317 39 136 312 330 486 213 209 Cuts at: 3872 830 Size: 3478 Fragments arranged by size: 3478 1317 486 330 312 213 209 136 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6520 PvuII CAG'CTG Cuts at: 2332 2404 2725 2965 2989 3192 2332 Size: 72 321 240 24 203 5660 Fragments arranged by size: 5660 321 240 203 72 24 SacI G_AGCT'C Cuts at: 2172 2664 3321 2172 Size: 492 657 5371 Fragments arranged by size: 5371 657 492 SacII CC_GC'GG Cuts at: 2578 2578 Size: 6520 SalI G'TCGA_C Cuts at: 1936 3862 1936 Size: 1926 4594 SapI GCTCTTCn'nnn_ Cuts at: 2504 2648 2504 Size: 144 6376 SbfI CC_TGCA'GG Cuts at: 2322 3872 2322 Size: 1550 4970 ScaI AGT'ACT Cuts at: 4768 6480 4768 Size: 1712 4808 SfcI C'TryA_G Cuts at: 826 1045 2143 2182 2318 2630 2960 3446 3659 Size: 219 1098 39 136 312 330 486 213 Cuts at: 3659 3868 5003 5684 5875 826 Size: 209 1135 681 191 1471 Fragments arranged by size: 1471 1135 1098 681 486 330 312 219 213 209 191 136 39 SfoI GGC'GCC Cuts at: 2785 2785 Size: 6520 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6520 SmaI CCC'GGG Cuts at: 1872 2223 2781 3582 3847 1872 Size: 351 558 801 265 4545 Fragments arranged by size: 4545 801 558 351 265 SmlI C'TyrA_G Cuts at: 820 1017 1943 3023 3254 3635 4624 5525 5787 Size: 197 926 1080 231 381 989 901 262 Cuts at: 5787 820 Size: 1553 Fragments arranged by size: 1553 1080 989 926 901 381 262 231 197 SnaBI TAC'GTA Cuts at: 493 4311 493 Size: 3818 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6520 SphI G_CATG'C Cuts at: 1132 3878 1132 Size: 2746 3774 SspI AAT'ATT Cuts at: 5 52 4444 5 Size: 47 4392 2081 Fragments arranged by size: 4392 2081 47 StuI AGG'CCT Cuts at: 1517 2025 1517 Size: 508 6012 StyI C'CwwG_G Cuts at: 513 1065 2004 3959 513 Size: 552 939 1955 3074 Fragments arranged by size: 3074 1955 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2044 2246 4547 4564 4717 4902 5347 Size: 1255 464 202 2301 17 153 185 445 Cuts at: 5347 325 Size: 1498 Fragments arranged by size: 2301 1498 1255 464 445 202 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4766 6478 96 Size: 274 80 33 51 1443 2789 1712 138 Fragments arranged by size: 2789 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3581 3613 6480 6512 3581 Size: 32 2867 32 3589 Fragments arranged by size: 3589 2867 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2216 1316 Size: 900 5620 XbaI T'CTAG_A Cuts at: 3856 3856 Size: 6520 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3505 3505 Size: 6520 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6520 XmaI C'CCGG_G Cuts at: 1870 2221 2779 3580 3845 1870 Size: 351 558 801 265 4545 Fragments arranged by size: 4545 801 558 351 265 XmnI GAAnn'nnTTC Cuts at: 4649 4649 Size: 6520 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3469 4328 276 Size: 53 83 186 936 1935 859 2468 Fragments arranged by size: 2468 1935 936 859 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI